Abstract

A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120 rugged roses from 6 different Chinese source populations based on CDDP marker, and a preliminary construction of the core collection was established using stepwise UPGMA clustering sampling method. The 26 core collection resources of R. rugosa collections in China have 20% germplasm samples of initial collection, the retention ratio of polymorphic loci, effective number of alleles (Ne), Nei’s genetic diversity (H) and Shannon information index (I) were respectively 97.52%, 104.16%, 108.38% and 106.18%. The results of t-test showed that no significant difference was found in genetic diversity indexes between the core collection and the original collection. These results also demonstrated that the core collection could stand for original collection excellently. The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses.

Highlights

  • Rosa rugosa, a deciduous shrub of the genus Rosa, has prickly stems, pinnately compound leaves, and variously colored, often fragrant flowers

  • The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses

  • This suggests that rugged roses are rich in genetic diversity

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Summary

Introduction

A deciduous shrub of the genus Rosa, has prickly stems, pinnately compound leaves, and variously colored, often fragrant flowers. The rugged rose is a deciduous shrub native to northeastern China and adjacent areas of North Korea, Japan and eastern Russia [1] [2]. As R. rugosa is harder than most cultivated roses, its germplasm is an important resource for rose cultivation. This species is important ecologically, as it can grow on sandy substrates and protect delicate coastal areas from erosion. In order to protect endemic populations of this ecologically important plant in China better, it is necessary to have a deep understanding of genetic variation and establish a core collection among various rugged rose populations. The aim of this study is to establish core germplasm resources of rugged rose with CDDP molecular marker technology

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