A multibiomarker approach for evaluating environmental contamination: Common carp (Cyprinus carpio) transplanted along a gradient of metal pollution

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Environmental monitoring and risk assessment approaches which include a more holistic view on the effects of pollutants on biota are increasingly sought by regulators and policy makers. Therefore, caged carp juveniles (Cyprinus carpio) were transplanted for 7 weeks along a known Cd and Zn pollution gradient. Metal (Cu, Cd and Zn) accumulation in gill and liver tissue and effect biomarkers (growth, condition factor (CF), hepatosomatic index (HSI), oxygen consumption, swimming capacity, Na+/K+-ATPase activity (NKA) and metallothionein (MT) levels) were compared.Up to 10-fold higher cadmium concentrations were measured in the gills of the fish at the most polluted locations compared to the laboratory control fish. Similarly, cadmium concentrations in liver tissues of field-exposed fish were significantly higher than those measured in laboratory control fish. Cu and Zn concentrations in the gills were not significantly different between field-exposed and control organisms, whereas higher levels in liver tissues were measured in carps deployed in some locations. Effects on liver MT levels were up to 10 times greater for organisms exposed to the field, whereas no clear effect of the metal exposure on NKA in the gill tissue was observed. A decrease in muscle glycogen stores was observed for all organisms deployed in the field, while liver glycogen levels decreased only in fish exposed to two of the 5 sites compared to the laboratory control fish. Additionally, significant drops in liver protein- and lipid stores were observed. No effect on oxygen consumption rates and swimming capacity was observed. The CF and HSI of caged fish reflected the pollution gradient in the river and considerable loss of weight was observed for fish transplanted in the most polluted site. Overall, this active biomonitoring study successfully revealed differences in metal accumulation, physiological and organismal endpoints as a direct consequence of field exposure.