A Fundamental Structure of the General Theory of Overload Quasi-Static Linear Gradient Chromatography

Abstract

Abstract The earlier theory of overload linear gradient chromatography developed over 15 years ago by using an ideal molecular model with infinite dimensions is rearranged and improved in order for it to be compatible with the new concepts that are involved in the general theory of gradient chromatography developed recently. By applying the ideal molecular model, the structure of the overload chromatographic theory is considerably reduced, emphasizing the fundamental structure. Chromatograms with a shape close to a right-angled triangle with tailing are often experienced under overload condition. On the basis of the present theory, this type of chromatogram can be explained in terms of the occurrence of a quasi-crystalline phase of the adsorbed molecules that are repulsively interacting with one another on the adsorbent surfaces in the column.