Abstract
Abstract The earlier theory of quasi-static linear gradient chromatography is still generalized. The present theory is valid whatever may be the initial location of the sample molecules on the column, and the condition imposed upon the quasistatic chromatography is less severe in this theory than earlier. The earlier theory represents a special case of the present theory. The universal continuity equation for any quasi-static chromatography is. derived, from which the continuity equation that causally describes the chromatographic process can be derived for both gradient and isocratic (or stepwise) chromatographies.
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