Abstract

The various models of linear chromatograpliy are compared. Those include the discrete and continuous plate models, the stocchastic model, the equilibrium-dispersive model of chromatography, the lumped kinetic models, and the general rate model. We show that all these models are equivalent and can be correctly approximated by a Gaussian profile, even when the column efficiency is as low as 100 theoretical plates. The sources of band asymmetry and tailing in linear chromatography are also discussed. We show that the existence of active sites on the adsorbent surface is probably the main reason of band asymmetry, in most cases.

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