Abstract
Identification of cis/trans-carbon–carbon double-bond (CC) isomers remain challenging. Herein, a simple and rapid method for the separation and analysis of cis/trans-maleic acid (MA) and aconitic acid (AA) using Trapped Ion Mobility Spectrometry (TIMS) was developed. α-, β-, γ-cyclodextrin (CD) were served as the separation reagent, slight difference in mobility separation was obtained by [CD-MA/AA-H]-. Specially, with the addition of divalent metal ion (G2+) as coordination metal ion, the separation effect was much increased by [CD-MA/AA + G-H]+, and α-CD has better mobility separation effect than β-/γ-CD. Moreover, chemical calculations revealed the binary and ternary complexes are in the inclusion forms, and microscopic interactions between cis/trans-MA/AA, CDs, and G2+ are somewhat different that making their mobility separation. Finally, quantifications of cis/trans-isomers were analyzed in food samples, with good linearity (R2 > 0.99) and recoveries obtained from 87.25 % to 100.73 %.
Published Version
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