892 INTERMITTENT CALORIC RESTRICTION MODIFIES NEUROBIOLOGICAL RESPONSE TO BILATERAL CAVERNOUS NERVE CRUSH INJURY IN THE RAT AND FACILITATES RECOVERY OF ERECTILE FUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 2010892 INTERMITTENT CALORIC RESTRICTION MODIFIES NEUROBIOLOGICAL RESPONSE TO BILATERAL CAVERNOUS NERVE CRUSH INJURY IN THE RAT AND FACILITATES RECOVERY OF ERECTILE FUNCTION Robert Payne, Elaine Coderre, Ward T. Plunet, Jeffrey J.L. Carson, Leo P. Renaud, Wolfram Tetzlaff, Rany Shamloul, and Anthony J. Bella Robert PayneRobert Payne Ottawa, Canada More articles by this author , Elaine CoderreElaine Coderre Ottawa, Canada More articles by this author , Ward T. PlunetWard T. Plunet Vancouver, Canada More articles by this author , Jeffrey J.L. CarsonJeffrey J.L. Carson London, Canada More articles by this author , Leo P. RenaudLeo P. Renaud Ottawa, Canada More articles by this author , Wolfram TetzlaffWolfram Tetzlaff Vancouver, Canada More articles by this author , Rany ShamloulRany Shamloul Ottawa, Canada More articles by this author , and Anthony J. BellaAnthony J. Bella Ottawa, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1648AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Preclinical data for therapeutic dietary restriction has been shown to improve outcomes from cervical spinal cord injury. Candidate mechanisms include trkB, the receptor for brain-derived nerve growth factor (BDNF) which is a key cavernous nerve (CN) neuromodulator. The purpose of this study was to determine whether every-other-day-fasting (EODF), a form of intermittent caloric restriction, conferred an erectile recovery advantage following CN crush injury in the rat. METHODS 44 3-month old Sprague-Dawley male rats were used.Controls n=8and cohorts consisting of 9 animals divided into crush-injury only (no EODF), and treatment arms of EODF started 2 weeks prior to injury, EODF started at day of injury, and EODF initiated 2 weeks post-CN injury(EODF until intracavernous pressure measures). ICPchanges standardized to mean arterial pressure (MAP) at 5 months was measured and an extended treatment phase was chosen to wash out short term fasting effects. Tukey-Kramer test was used for post-hoc analysis. Proximal corpora were stained with primary antibodies against tyrosine hydroxylase, neuronal NO synthase (nNOS), and vesicular acetylcholine transporter (VaChT) as described by Nangle and Keast. RESULTS The mean maximal increase in ICP/MAP (with standard deviation) for control animals was 0.673 (.09) versus the crush-control cohort (no caloric modification) ICP/MAP change of 0.17 (0.06). Rats treated with EODF started two weeks prior to injury demonstrated significant (p<0.05) improvement in erectile function with ICP/MAP ratio of 0.37 (0.07) Fasting started day of injury demonstrated less robust recovery, with 0.28 (.05) compared to the “pre-treated” group. The final group, starting EODF two weeks post injury demonstrated results statistically similar to no caloric restriction, with a ICP/MAP ratio of .168 (0.06). The two primary neurobiological endpoints in this study, retrograde axonal transport of fluorogold to the major pelvic ganglion and cavernous body determination of nNOS and VaChT were significantly reduced in crush injury, and 2 week post-injury EODF, compared to EODF initiated 2 weeks prior to/and day of injury. CONCLUSIONS This is the first study to demonstrate endogenous stress-response neuromodulation in a model of radical prostatectomy induced CN injury. Intermittent caloric restriction in the form of EODF confers a recovery advantage for CN function post-injury as measured by ICP/MAP and intact parasympathic neurons. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e348-e349 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Robert Payne Ottawa, Canada More articles by this author Elaine Coderre Ottawa, Canada More articles by this author Ward T. Plunet Vancouver, Canada More articles by this author Jeffrey J.L. Carson London, Canada More articles by this author Leo P. Renaud Ottawa, Canada More articles by this author Wolfram Tetzlaff Vancouver, Canada More articles by this author Rany Shamloul Ottawa, Canada More articles by this author Anthony J. Bella Ottawa, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...