MP43-18 EARLY REGULAR TREATMENT WITH A CANNABINOID 2 RECEPTOR AGONIST PROTECTED ERECTILE FUNCTION IN A RAT MODEL OF CAVERNOUS NERVE CRUSH INJURY

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2014MP43-18 EARLY REGULAR TREATMENT WITH A CANNABINOID 2 RECEPTOR AGONIST PROTECTED ERECTILE FUNCTION IN A RAT MODEL OF CAVERNOUS NERVE CRUSH INJURY Ettore Di Trapani, Colicchia Michele, Alberto Briganti, Manuela Tutolo, Niccolò Maria Passoni, Francesco Montorsi, Fabio Benigni, and Petter Hedlund Ettore Di TrapaniEttore Di Trapani More articles by this author , Colicchia MicheleColicchia Michele More articles by this author , Alberto BrigantiAlberto Briganti More articles by this author , Manuela TutoloManuela Tutolo More articles by this author , Niccolò Maria PassoniNiccolò Maria Passoni More articles by this author , Francesco MontorsiFrancesco Montorsi More articles by this author , Fabio BenigniFabio Benigni More articles by this author , and Petter HedlundPetter Hedlund More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1175AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Despite the introduction of nerve-sparing radical prostatectomy (NSRP), erectile dysfunction (ED) is a common and challenging sequele. The ED in NSRP is caused by neurapraxia that causes penile hypoxia, loss of nerves, smooth muscle fibrosis and veno-occlusive dysfunction. Recent studies reported immunomodulatory and antifibrotic activity of cannabinoids. We aimed to test the effect of a cannabinoid-2 receptor agonist in a rat model for pelvic neurapraxia Methods After ethical approval, Sprague Dawley rats (n=30, 250-300 g) were A) sham-operated (n=10); or subjected to bilateral cavernous-nerve (CN) crush injury (CNI) and B) treated with JWH-133 (1mg/kg, intraperitoneal, N=12), or C) vehicle (intraperitoneal, N=12). Treatment was initiated 3 days before surgery until 7 days thereafter. Mean arterial blood pressure (MAP) was measured by catheterizing carotid artery and erectile function by direct measurements of intracavernous pressure (ICP) during electrical stimulation (2, 4 and 6V) of the CN. Statistical analysis was performed using an ANOVA (Newman Keuls). Values are mean±SEM. Results Basal ICP was similar in the 3 groups and amounted to 16±2.2, 22±3.2, and 18±1.8cmH2O for sham, CNI + vehicle, and CNI + JWH-133, respectively. MAP amounted to 133±8 (sham), 137±8 (CNI + vehicle), and 125±9cmH2O (CNI+JWH-133). Vehicle treated rats with CNI exhibited at any stimulation parameter significantly lower erectile responses recorded as ICP/MAP than both sham and CNI rats treated with JWH-133. The ICP/MAP values for 2, 4, and 6V were 34.4±4.2, 42.3±4.7, and 46.3±5.4 for vehicle treated CNI rats (p<0.05 vs sham and JWH-133). Corresponding data for sham were 62.5±5, 70±4.3, and 75.1±7.1. ICP/MAP in CNI JWH-133 treated rats were not different from sham and amounted to 51.4±5.4, 74.6±10, and 87.2±11.8cmH2O/second. Corresponding AUC values for CNI rats treated with vehicle were lower (p<0.01-0.05) and amounted to 10.5±1.9, 14.6±1.9, and 17.9±2.5cmH2O/second. CNI rats treated with JWH-133 also exhibited lower AUC than sham (p<0.05) with values amounting to 19±5.4, 31.4±6, and 34±4.8cmH2O/second. At 4 and 6V, the AUC values of JWH-133-treated CNI rats were higher (p<0.05) than those of vehicle-treated CNI rats. Conclusions Early initiated perioperative regular treatment for 10 days with the CB2 agonist JWH-133 restored maximal ICP during erections in rats with CNI but only partially improved the total erectile responses recorded as the AUC. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e484-e485 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Ettore Di Trapani More articles by this author Colicchia Michele More articles by this author Alberto Briganti More articles by this author Manuela Tutolo More articles by this author Niccolò Maria Passoni More articles by this author Francesco Montorsi More articles by this author Fabio Benigni More articles by this author Petter Hedlund More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...