Abstract
The cellular proteins of Saccharomyces cerevisiae have been labelled continuously during vegetative growth using 35SO42-. During subsequent sporulation in sulphur-free sporulation medium the proteins were analysed using two-dimensional polyacrylamide gel electrophoresis. Previously it has been shown that about half of the changes found in the detectable polypeptides were specific to the sporulation process. The changes may be due to either de novo synthesis or modification of existing proteins. The present work reports that these changes occur at characteristic times throughout sporulation with some detectable very soon after transfer to sporulation medium. The bulk of the changes seem to occur within the first eight hours of sporulation.
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