Abstract
A wide array of molecular subtyping approaches is currently being used to characterize foodborne pathogens in order to assist epidemiologists with cluster detection and trace back studies during outbreak investigations. The data generated by these methods are often used to populate reference databases or libraries. Once a new method has been developed or an old one adapted for this purpose, it must be subjected to the highest scrutiny possible to ensure that the data that will be generated meet the criteria and standard expected of a reference library. In this chapter, we will outline a typical development, validation and quality assurance process for a method to be used in the PulseNet subtyping network.
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