ВОЗРАСТНЫЕ ОСОБЕННОСТИ МОРФОГЕНЕТИЧЕСКИХ СВОЙСТВ ЛИМФОИДНЫХ КЛЕТОК СЕЛЕЗЕНКИ

Abstract

Aim. The aim of the study is to determine the features of the morphogenetic properties of the spleen lymphoid cells in animals of different age groups. Materials and research methods. In the experiments, a model of adoptive transfer of lymphoid cells of the spleen of nephrectomized animals from different age groups was used. The studies were carried out on male Wistar rats of mature (8-10 months, weighing 200-250 g) and old (19-22 months, weighing 400-500 g) age. Study groups: control group — model of adoptive transfer of splenocytes from mature animals to mature ones; second — experimental group: model of adaptive transfer of lymphoid cells from old — old animals; the third group — transplantation of lymphoid cells from old animals — to mature ones; the fourth group — transplantation of lymphoid cells from mature animals — to old ones. The morphogenetic properties of the spleen cells of nephrectomized animals from different age groups were studied 19 hours after unilateral nephrectomy (donor interval). The resulting suspension was injected intravenously into recipients at 400×10³ cells per 0.2 kg of animal weight. 8 hours before slaughter, the animals received a single intraperitoneal dose of 2 mg/kg of vinblastine. Recipients were sacrificed 40, 48 and 56 hours after splenocyte transfer. Research results. Transplantation of splenocytes obtained from old donors to old recipients did not reveal any stimulation of proliferative processes in the epithelium of the kidney tubules of the latter. The transfer of the same splenocytes to mature donors is accompanied by the manifestation of their morphogenetic function. At the same time, the activity of proliferative processes in the kidneys of the recipients was not less pronounced than in the control group.