ПЕРЕКИСНЕ ОКИСНЕННЯ ЛІПІДІВ У ХРЯЩОВІЙ ТКАНИНІ ЩУРІВ ЗА УМОВ ЕКСПЕРИМЕНТАЛЬНОГО ОСТЕОАРТРИТУ ТА ПРИ ВВЕДЕННІ МУЛЬТИПРОБІОТИКА

Abstract

The aim of the study was to investigate the effect of multiprobiotic on the content of lipid peroxidation products in rat cartilage during monoiodoacetate-induced osteoarthritis. The study was carried out on white non-linear, sexually mature male rats (weight 180-240g), according to general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – Control: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The second group – Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the protocol of the ethics committee with rapid blood sampling. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-dinitrophenylhydrazine. The content of diene conjugates was determined in the heptane-isopropanol extract by the spectrophotometric method, and of Schiff bases – by the fluorimetric method. The content of TBK-active compounds was determined by reaction with thiobarbituric acid. It has been established that MIA-induced OA the content of lipid peroxidation products (diene conjugates, TBK-active compounds, schiff bases) increases in the cartilage. It was shown that with the administration of multiprobiotic in animals with MIA-induced OA, the above indicators were restored.