블루라이트 차단 효과 평가를 위한 동물대체시험 모델의 응용

Abstract

Blue light has a wavelength of 400 to 500 nm and is widely recognized as a cause of harm to skin health in modern society where the use of electronic devices emitting blue light is common. In this study, an alternative animal model is introduced as a way to characterize the blue light blocking effect. Based on a theoretical background, blue light was irradiated to zebrafish from the embryo stage, and then the degree of melanin formation was observed to investigate the potential of the new research method. As a result, the blue light irradiation group showed a high pigmentation rate at 12 and 24 hpf. In the experimental group irradiated with fluorescent light, the pigmentation rate was 54% lower than that of the blue light irradiated group. The fluorescent light and negative control group, the light-shielded experimental group, had a lower melanin pigmentation rate than the fluorescent light and blue light irradiation groups. Whitening research using zebrafish has the advantage of simultaneously testing toxicity and whitening, making it a highly efficient evaluation method and thus highly likely to be used in the cosmetics industry as an in vivo experimental model that can replace existing animal experiments.