Клинико-гистологические особенности заживления язв роговицы у кроликов

Abstract

Ulcerative lesions of the cornea account for 47 to 70 % of all corneal diseases. In the case of a favorable course of corneal ulcers, opacification of varying degrees of intensity is formed. The currently existing methods of treating this pathology are not always effective, so the search for adequate models for experimental studies of new drugs is relevant. Purpose. To study the clinical and histological features of the healing of a corneal ulcer in rabbits. Material and methods. Animals were randomly divided into three groups of 29 rabbits (58 eyes) each. Rabbits of the first group received traditional treatment. Rabbits of the second and third groups underwent intrastromal administration of an antibiotic. The third group, against the background of traditional treatment, received a combination of intrastromal administration of an antibiotic into the cornea with PRP instillation. Animals underwent euthanasia on the 3rd, 5th, 10th, 14th and 21st days after infection of the cornea, 1 and 6 months after treatment. The results of the study showed that the timing of the decrease in the inflammatory response and the timing of the disappearance of purulent discharge in all three groups coincided (7th day) and amounted to 25.9 ± 5.7 %, 43.1 ± 6.5 % and 70.7 ± 6.0 % of cases, respectively. The number of epithelized ulcers by the 14th day of treatment in rabbits of the third groups is 3.4 and 1.7 times more than in rabbits of the 1st and 2nd groups, respectively. Histomorphological studies have shown that the inclusion of PRP in the complex therapy of corneal ulcers in the early stages of the inflammatory process in the cornea (the phase of alteration and exudation) leads to a reduction in the time of relief of the inflammatory process, accelerates the time of corneal epithelization. Keywords: corneal ulcer, PRP, intrastromal administration