Inhibition Zone Research Articles

Extraction and Activity Test of Antibacterial Compounds from Limberry Leaves (Triphasia Trifolia) Against Vibrio Sp.

Limberry (Triphasia trifolia) is one of traditional medicinal plants whereas its leaves are known for various uses, one of which is as an antibacterial agent. The most common bacteria found in marine waters is Vibrio sp. This work seeks to distinguish antibacterial compounds inside the ethanol extract of limberry leaves and assess its repressive effect on Vibrio sp. The research was conducted with three repetitions and five different concentration levels: D1 (5%), D2 (10%), D3 (15%), D4 (20%), and D5 (25%). The observational data were analyzed using a completely randomized design (CRD) analysis of variance, followed by the least significant difference (LSD) test at a 5% significance level. Phytochemical screening revealed that the ethanol extract of limberry leaves contained antibacterial compounds namely flavonoids, tannins, steroids, alkaloids, and phenolics. UV-Vis spectrophotometry analysis indicated that the ethanol extract of limberry leaves had absorption at wavelengths of 202, 211, 228, 253, 270, and 323 nm. FTIR analysis showed that the ethanol extract of limberry leaves contained some functional groups, i.e., O–H, C–H alkanes, C–H alkenes C≡N, C=O, C=C, C–O esters, and C–O ethers. The inhibitory test also demonstrated that the ethanol extract of limberry leaves could inhibit the growth of Vibrio sp., with inhibition zone diameters formed at each concentration of 5%, 10%, 15%, 20%, and 25% being 7.280, 7.640, 7.713, 8.027, and 8.070 mm, respectively.

Synthesis, characterization and biological activity of the Cu (II)-1,3-PDTA [(Cu (II) (PDTA)] complex for the control of Erwinia chrysanthemi (soft rot) in the parsley manioc (Arracacia xanthorrhiza Bancroft)

Synthesis, characterization and biological activity of the Cu(II)-1,3-PDTA complex was performed by two synthetic routes: sodium and carbonate pathways. The Cu (II)-1,3-PDTA complex can be easily produced by the 1,3-PDTA binder, commercially available as Trilon F (BASF) which is available as sodium or acid salt for the coordination of Cu (II). The biological activity of the Cu (II)-1,3-PDTA [Cu (II)-PDTA] complex was performed to analyze the activity against the bacterium Erwinia chrysanthemi Bancroft. The bacterium is the causative agent of soft rot in the parsley maniac (Arracacia xanthorrhiza Bancroft). The bacterium was isolated from parsley manioc infected with soft rot. The metal complex sensitivity tests were performed by diffusion antibiogram. The mean values of the halos and standard deviations of the zones of inhibition were obtained for the [Cu (II) (PDTA)] and Streptomycin complexes. The concentrations of the complexes evaluated were 10-3, 10-2 and 10-1 M (equivalent to 36.3 g L-1 (3.63%), 3.63 g L-1 (0.363%) and 0.363 g L-1 (0.0363%), respectively). The halos were 25.0 ± 1.2 mm for control. For [Cu (II) (PDTA)] complexes, the obtained values were 9.5 ± 0.6, 15.0 ± 0.8 and 24.0 ± 0.8 via sodium and 12.0 ± 1.6, 25.0 ± 0.8 and 31.0 ± 1.9 via carbonate, for 10-3, 10-2 and 10-1 M, respectively. The results showed that the bactericidal activity of the Cu (II)-1,3-PDTA complex obtained by the two synthetic routes are adequate for the control of Erwinia chrysanthemi Bancroft.

Unveiling the multifaceted bioactivity of copper(II)-Schiff base complexes: a comprehensive study of antioxidant, anti-bacterial, anti-inflammatory, enzyme inhibition and cytotoxic potentials with DFT insights.

The rise of various diseases demands the development of new agents with antioxidant, antimicrobial, anti-inflammatory, enzyme-inhibiting, and cytotoxic properties. In this study, heterocyclic Schiff base complexes of Cu(II) featuring a benzo[b]thiophene moiety were synthesized and their biological activities evaluated. The complexes were characterized using FT-IR, UV-Vis, and EPR spectroscopy, TG-DTG analysis, magnetic moment measurements, molar conductivity measurements, and elemental analyses. Density functional theory (DFT) calculations were used to optimize the theoretical molecular orbital energies of the copper complexes. The complexes exhibited square pyramidal and square planar geometries. Biological assays demonstrated that these complexes generally outperformed the Schiff base ligands for various activities. The antioxidant capacity, measured via the DPPH assay in methanol, was comparable to those of the BHT and ascorbic acid standards, with 4BNPC showing the lowest IC50 value, which was attributed to the free OH group rather than coordination to the metal center. The anti-bacterial activity was assessed using the agar disc diffusion method against E. coli, P. aeruginosa, B. subtilis, and S. aureus, with BAC showing the largest inhibition zone compared to the others and ciprofloxacin as the reference. The anti-inflammatory activity, evaluated by the HRBC membrane stabilization method, showed that the 4BNPC Cu(II) complex had moderate activity similar to that of diclofenac. Enzyme inhibition studies against α-amylase revealed that the BAC complexes had the highest inhibition values, surpassing those of the Schiff base ligands. Cytotoxicity was assessed using Trypan blue exclusion for DLA and HepG2 cancer cell lines, and the MTT assay for H9c2 human cells. BMPC demonstrated superior cytotoxicity at both high and low concentrations against the normal H9c2 cell line. Among the tested compounds, BNPC showed moderate inhibition against HepG2 cells, while BMPC exhibited the greatest cytotoxicity at higher concentrations, particularly reaching nearly 100% cell death at 200 μg mL-1 in DLA cell lines. This suggests that BMPC is a promising candidate for further pharmacological research, particularly against DLA cells.

SYNTHESIS OF VANADIUM GRAPHITIC-CARBON NITRIDE(V/g-C₃N₄) COMPOSITE FOR BIOLOGICAL APPLICATION

The synthesis of vanadium carbon nitride (V/g-C₃N₄) composites has been explored for their potential as antimicrobial and antioxidant agents. These materials were evaluated against Escherichia coli and Staphylococcus aureus using a combination of disc diffusion, well diffusion, and biofilm inhibition assays. Antioxidant properties were assessed through DPPH free radical scavenging analysis. V/g-C₃N₄ composites demonstrated notable antimicrobial activity, exhibiting inhibition zones comparable to ciprofloxacin, the standard antibiotic. In disc and well diffusion assays, the composites significantly inhibited the growth of both bacterial strains, with Staphylococcus aureus exhibiting marginally higher sensitivity. Biofilm inhibition assays revealed effective suppression of bacterial adhesion and biofilm formation, achieving biofilm inhibition percentages of approximately 17% for E. coli and 22.6% for S. aureus. These results underscore the potential of V/g-C₃N₄ in curbing biofilm-associated infections. Antioxidant activity was quantitatively measured using the DPPH assay, with radical scavenging efficiency consistently exceeding 52%. This performance highlights the dual functionality of V/g-C₃N₄ composites as both antimicrobial and antioxidant agents. Comparatively, ciprofloxacin demonstrated superior antibacterial activity but lacked antioxidant properties, emphasizing the multifaceted advantage of V/g-C₃N₄ composites in biomedical applications. The shake-flask method further validated the biocompatibility and antimicrobial effectiveness of V/g-C₃N₄. This study indicates that vanadium carbon nitride composites hold promise as versatile agents in combating oxidative stress and bacterial infections, particularly in environments where biofilm formation exacerbates antimicrobial resistance. Future studies will focus on optimizing the composite synthesis and exploring its efficacy against a broader range of pathogens.

Inhibitory effect of magnetic aqueous extract of Syzygium aromaticum L on two types of oral pathogenic bacteria

<p><strong>Aim</strong> Magnetic fields affect all living things, which is why they are the subject of extensive research around the world. The aim of this study was to investigate the effects of different levels of static magnetic field on chemical behaviours of aqueous extract of <em>Syzygium aromaticum</em> L and their effect on selective oral pathogenic bacteria. <br /><strong>Methods</strong> This study utilized locally manufactured dipolar static magnetic fields with strengths between 1200 and 3200 Gauss, measured by a Sylocimol Residence magnetizer. Gram positive G (+) <em>Streptococcus viridans</em> and G negative (G-) <em>Porphyromonas gingivalis</em> strains were used in studies on the antimicrobial properties of <em>S. aromaticum</em> extract. Tests were carried out using the clove essential component (eugenol) and its functionalized extract to evaluate various bacterial inhibitions and establish whether there is a synergistic effect between the extract and subjected to the magnetic field. <br /><strong>Results</strong> Polyphenol extraction increased with eugenol content. The analytical method's reliability was confirmed by chromatograms of eugenol and eugenol acetate standards, demonstrating good separation of compounds. The selectivity of the method was guaranteed by the purity index of the eugenol and eugenol acetate peaks. The aqueous extract of <em>S. aromaticum</em> showed magnetic properties. The extract showed antimicrobial activity against two bacterial strains. Cell viability changed at different magnetic field intensities, and the inhibition zone for <em>P. gingivalis</em> increased significantly due to prolonged exposure.  <br /><strong>Conclusion</strong> The magnetized extract inhibits both G (+) and G (-) bacteria when combined with clove extract.</p>

Phytochemical Screening, Antioxidant Test and Antimicrobial Test of Streptococcus Mutans and Candida Albicans from Extracts of Ultrasound Assisted Extraction of Red Betel Leaves (Piper Ornatum N. E. Br)

Red betel (Piper ornatum) is an ornamental plant with potential antibacterial and antioxidant properties due to its bioactive compounds, including alkaloids, flavonoids, steroids, triterpenoids, saponins, and tannins. This study aimed to determine the relationship between flavonoid content, antioxidant activity, and antimicrobial activity in red betel leaves. Ultrasound-Assisted Extraction (UAE) was employed using three solvents of varying polarity: n-hexane (non-polar), ethyl acetate (semi-polar), and ethanol (polar). Total flavonoid content was determined spectrophotometrically based on flavonoids' ability to form complexes with AlCl3. Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Antibacterial and antifungal properties were evaluated through Minimum Inhibitory Concentration (MIC) and Zone of Inhibition (ZOI) tests. MIC was determined using the agar dilution method, while ZOI was measured using the disc diffusion method. Results showed that the 96% ethanol extract yielded the highest flavonoid content (4.748%) and the strongest antioxidant activity (IC50 = 47.185 ppm, classified as very strong). The n-hexane extract at 25% concentration exhibited the best antibacterial activity against Streptococcus mutans (ZOI = 17.43 mm), while the 96% ethanol extract at 25% concentration demonstrated the highest antifungal activity against Candida albicans (ZOI = 17.02 mm). In conclusion, red betel leaf extracts show promising potential as strong antioxidants and antimicrobial agents against C. albicans and S. mutans.

Evaluating the antibacterial efficacy of bee venom against multidrug-resistant pathogenic bacteria: Escherichia coli, Salmonella typhimurium, and Enterococcus faecalis

Bee venom (BV) represents a promising natural alternative to conventional antibiotics, particularly significant given its broad-spectrum antimicrobial activity and potential to address the growing challenge of antimicrobial resistance. The prevalence of antimicrobial-resistant microorganisms (AMR) is a global burden that affects human health and the economies of different countries. As a result, several scientific communities around the world are searching for safe alternatives to antibiotics. In this context, the present study represents a comprehensive investigation to evaluate the antibacterial effect of bee venom (BV) against Escherichia coli ATCC8739, Salmonella typhimurium ATCC14028, and Enterococcus faecalis ATCC25923. One mg of BV was extracted using 1 mL of DMSO to obtain a 1000 µg/mL solution. The chemical profile of the BV extract was determined using GC-MS, which revealed the presence of bioactive molecules with antimicrobial properties, such as astaxanthin, hycanthone, and fucoxanthin. The BV extract was tested against bacterial strains using different concentrations to obtain the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results obtained revealed a high antibacterial activity of BV against the three strains with the highest MIC/MBC values of 12.5/25 µgml− 1 against S. typhimurium. The antibacterial activity of the BV extract was compared to five conventional antibiotics using the disc diffusion method. The results showed a high antibacterial activity of the BV extract compared to different antibiotics with the largest inhibition zone obtained against E. faecalis at a value of 15 ± 0.22 mm compared to 9 ± 0.13 for azithromycin. The mode of action of BV, examined using scanning electron microscopy, proved a high effect of BV on the permeability of the bacterial plasma membrane. This study demonstrates bee venom’s promising potential as a natural and eco-friendly antimicrobial agent, with activity against multiple bacterial strains, suggesting it may serve as an alternative to conventional antibiotics. The findings highlight the potential applications of BV in medical, agricultural, and veterinary fields, offering a sustainable solution to combat antimicrobial resistance. However, further studies are needed to fully assess its broad-spectrum antibacterial potential.Clinical trial number Not applicable.

Resistance Profile of Strains of Escherichia coli and Salmonella sp. Isolated from Raw Cow Milk Sold in the Department of Vina in Cameroon

Aims: Antimicrobial resistance, defined by the WHO as “resistance of a micro-organism to an antibiotic to which it was previously sensitive”, results from the ability of the bacterium to withstand the attack of antibiotics. This resistance is a major factor in the risk of therapeutic failure and the spread of multidrug-resistant strains from animals to humans. The present study was aimed to assess the antibiotic resistance of strains of Escherichia coli and Salmonella sp. Isolated from cattle farms in the Department of Vina, Cameroon. Place and Duration of Study: The study involved 30 strains of Salmonella (05) and Escherichia coli (25) isolated from 60 samples of raw cow milk, collected from July to August 2021 in Vina, Adamawa Province. The samples were each made up of 15 ml of milk, taken aseptically from cattle farms and sales depots. Methodology: Antibiotic susceptibility of 25 strains of Escherichia coli and 5 Salmonella sp. strains was wanted, according to the standard Mueller-Hinton agar diffusion method 16 antibiotic discs. Results: The diameters of the inhibition zones formed showed fairly high resistance rates, 87.5% for Salmonella sp. and 75% for E. coli. Bacterial strains are all resistant to the Penicillin, Macrolides and Diaminopyrimidines tested. Good sensitivity was observed with levofloxacin (100%), cefixime (82.14%) and gentamycin (78.60%). Conclusion: The importance of these resistances reflects the intensive previous use of antibiotics in livestock farming as growth-promoting additives.

ASSESSMENT OF BACTERICIDAL ROLE OF EPIDERMAL MUCUS OF MAJOR CARPS AGAINST PATHOGENIC MICROBIAL STRAINS

This research evaluated the antimicrobial potential of mucus from major carps, specifically Cirrhinus mrigala and Labeo rohita, against Klebsiella pneumoniae and Escherichia coli. Fish weighing 300-350 grams were stoked and acclimated for 15 days, fed with commercially available diet at 4% of their body weight. Fish were treated with KMnO4 solution to avoid any infection before mucus collection and collected mucus was stored at 4°C. Mucus extracts were screened for antibacterial potential using the agar well diffusion method, measuring antibacterial effects by the zone of inhibition (ZOI) in mm. L. rohita secreted more mucus than C. mrigala. The mucus appearance of L. rohita was highly viscous, while less viscous in C. mrigala. In L. rohita mucus, maximum antimicrobial efficiency was observed. Results showed greater efficacy in limiting the growth of E. coli with zone of inhibition (16mm). Mucus is a key defense against disease. Fish skin mucus can serve as an alternative to antibiotics for use in aquaculture and potentially for human application. As a natural product, it may help reduce problems associated with antibiotic resistance.

Additive Effects of Natural Plant Extracts/Essential Oils and Probiotics as an Antipathogenic Topical Skin Patch Solution for Acne and Eczema.

This work leverages the additive antipathogenic effects of natural extracts/essential oils (EOs) and probiotics for the treatment of acne vulgaris associated with Cutibacterium acnes (C. acnes) and eczema complicated by secondary infections with Staphylococcus aureus (S. aureus). Six probiotic strains and various extracts/EOs were evaluated in a large screening to evaluate their potential against both pathogens. Lacticaseibacillus paracasei PCB003 was able to inhibit the growth of both pathogens. For extracts/EOs, Oregano EO had the best antipathogenic effects on both pathogens and did not show any adverse impact on the growth of probiotics, making it suitable for simultaneous use. Using Lactiplantibacillus plantarum PCB011 as a probiotic model, five material formulations were assessed for their suitability to protect probiotic cells within freeze-dried topical patches. Alginate and trehalose (ALG+TRE) and thermoplastic starch (TPS) had the highest probiotic survivability, with ALG+TRE chosen as the final patch material as it was more robust. PCB003 and PCB011 were individually incorporated into the ALG+TRE freeze-dried matrix to form a 6 mm patch; both ALG+TRE (PCB003) and ALG+TRE (PCB011) patches, when used individually, successfully inhibited C. acnes growth by 4.7 and 6.0 mm, respectively, surpassing the performance of commercially available acne patches. The additive effect with 30% Oregano EO further improved pathogen inhibition. For S. aureus, the incorporation of 30% Oregano EO to ALG+TRE (PCB003) increased the size of the inhibition zone more than 10-fold. For C. acnes, the ALG+TRE (PCB003) patch with 30% Oregano EO demonstrated an inhibition zone of 16.3 mm, and the ALG+TRE (PCB011) patch with 30% Oregano EO achieved a 14.3 mm inhibition zone. Genomic analysis confirmed that PCB003 and PCB011 lack antimicrobial resistance determinants, ensuring safety. This study successfully combined probiotics and natural agents to create effective dermatological antipathogenic patches.

Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities

Nanotechnology has experienced significant advancements, attracting considerable attention in various biomedical applications. This innovative study synthesizes and characterizes Ge/PLA/AuNCs (gelatin/PLA/gold nanocomposites) using Syzygium cumini extract to evaluate their various biomedical applications. The UV–Visible spectroscopy results in an absorption peak at 534 nm were primarily confirmed by Ge/PLA/AuNCs synthesis. The FTIR spectrum showed various functional groups and the XRD patterns confirmed the crystalline shape and structure of nanocomposites. The FESEM and HRTEM results showed a oval shape of Ge/PLA/AuNCs with an average particle size of 21 nm. The Ge/PLA/AuNC’s remarkable antioxidant activity, as evidenced by DPPH (70.84 ± 1.64%), ABTS activity (86.17 ± 1.96%), and reducing power activity (78.42 ± 1.48%) at a concentration of 100 μg/mL was observed. The zone of inhibition against Staphylococcus aureus (19.45 ± 0.89 mm) and Echericia coli (20.83 ± 0.97 mm) revealed the excellent antibacterial activity of Ge/PLA/AuNCs. The anti-diabetic activity of Ge/PLA/AuNCs was supported by inhibition of α-amylase (82.56 ± 1.49%) and α-glucosidase (80.27 ± 1.57%). The anti-Alzheimer activity was confirmed by inhibition of the AChE (76.37 ± 1.18%) and BChE (85.94 ± 1.38%) enzymes. In vivo studies of zebrafish embryos showed that Ge/PLA/AuNCs have excellent biocompatibility and nontoxicity. The SH-SY5Y cell line study demonstrated improved cell viability (95.27 ± 1.62%) and enhanced neuronal cell growth following Ge/PLA/AuNCs treatment. In conclusion, the present study highlights the cost-effective and non-toxic properties of Ge/PLA/AuNCs. Furthermore, it presents an attractive and promising approach for various future biomedical applications.

Aminated Poly (1, 4‐Phenylene Ether Ether Sulfone) Membrane Grafted With AgTiO2–r(OXSWCNTs‐GO) Nanocomposites With Antimicrobial Properties

ABSTRACTIn this study, we created antimicrobial membranes by grafting aminated poly(1,4‐phenylene ether ether sulfone) (APEES) with silver‐doped titanium dioxide nanoparticles (AgTiO2 NPs) and reduced oxidized single‐wall carbon nanotube‐graphene oxide r(OXSWCNT‐GO) nanocomposites to combat membrane biofouling, which is a major challenge in membrane technology. APEES was prepared by nitrating PEES and then reducing it to increase its hydrophilicity. Scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier‐transform infrared spectroscopy, and X‐ray diffraction spectroscopy were used to characterize the membranes. The antimicrobial properties of the tested membranes were found to be effective against gram‐negative bacteria, specifically Pseudomonas aeruginosa and Escherichia coli. The APEES–AgTiO2 and APEES–AgTiO2–r(OXSWCNT‐GO) nanocomposites showed larger inhibition zones than the polymers lacking Ag/TiO2. The APEES–AgTiO2 nanocomposite showed a maximum inhibition zone of 13 mm compared to the other polymers. Docking investigations confirmed these findings, revealing a strong fit of the tested polymer nanocomposites into DNA gyrase B (PDB ID: 4uro). The APEES–AgTiO2 nanocomposite had the highest docking score of −5.2728 kcal/mol, which corresponded with the observed antibacterial activity during practical testing.

Efficacy of Ethanol Extract of Sambiloto (Andrographis paniculata) Against Pneumonia-Causing Klebsiella pneumoniae

Klebsiella pneumoniae is one of the most common pathogens in nosocomial respiratory infections. Antibiotics are often used for the treatment of K. pneumoniae infections. Increased bacterial resistance to antibiotics is closely related to the success rate of infectious disease therapy. The use of plants as phytopharmacology is currently an alternative treatment. Some medicinal plants that have antibacterial and immunomodulatory activity are Sambiloto (Andrographis paniculata) with an active compound called andrographol. The purpose of this study was to provide a solution to the problem above by developing a phytopharmaceutical using bitter extract to inhibit the growth of K. pneumoniae bacteria. Two sputum samples from the laboratory confirmed Covid-19 patients with pneumonia infection at the Hospital. The sambiloto was extracted by using 96% ethanol. The extract was further analyzed for its antimicrobial activity and phytochemical compounds. There were two K. pneumoniae isolates, namely KpRNG and KpTRI. The active compounds were saponin and steroids. The antimicrobial activity of the ginger extract was measured with different concentrations of 25%, 50%, 75%, and 100%. Its inhibitory zone against K. pneumoniae was ranging from 5.8 to 7 mm or had a moderate inhibition category.

Antimicrobial Activities of Citrus Seed and Leaf Extracts Against Selected Bacterial and Fungal Pathogens Linked to Human Diseases: In vitro Experimental Study in Musanze, Rwanda

Antimicrobial resistance has caused major challenges in both bacterial and fungal infections. Therefore, the current study was designed to evaluate the antimicrobial activity with different crude extracts of leaves and seeds of Citrus limon against Gram-positive (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa) and fungi (Cryptococcus neoformans and Candida albicans) obtained from different clinical settings. Filtrate extracts after maceration technique and the phytochemical substances detected in plants were flavonoid, saponin, alkaloid, glycoside, and resin under investigation. In vitro, a disc diffusion assay was applied to measure the inhibition zone of the plant effect in millimeters (mm). It was observed that Citrus limon activity showed the highest on ethanolic seed against Staphylococcus aureus and the lowest activity plant observed on methanolic leaf extract against Klebsiella oxytoca. The mean values of Citrus limon extract convinced there was no statistically significant correlation among extract solvents used in the study (ρ > 0.05). Oxacillin was used as the positive control and discs without antibiotic content as the negative control during testing. To conclude, ethanolic seed extract has shown the highest effect against Staphylococcus aureus and Salmonella typhi from isolate. Indeed, plant extracts that showed potential effectiveness, such as (Citrus leaves and seeds) can be used as natural alternative preventive measures to control pathogenic bacterial diseases and prevent the rise of antibiotic resistance. Furthermore, studies to evaluate the in vivo potential in an animal model are recommended. Keywords: Citrus limon, Crude extract, Bacteria, Fungi, Antimicrobial resistance.

Influence of Zinc Salt Types on Physicochemical and Antimicrobial Properties of Zinc Stannate Nanostructures

Zinc stannate (ZTO) NPs were prepared hydrothermal for 20 hours at 200 ᵒC. Structural, morphological, and optical properties of the ZTO NPs were studied for different zinc salt types (ZnSO4.7H2O, Zn(CH3COO)2.2H2O, and Zn (NO3)2•6H2O). The X-ray diffraction studies showed the crystalline nature with a spinel crystal structure of the ZTO nanostructure. FE-SEM image showed spherical morphology with the formation of a network of aggregates. The transmittance spectra were recorded at wavelengths ranging from 200 nm to 1200 nm. In particular, their optical band gap energies were 3.65, 3.85, and 3.8 eV for three zinc salts of ZTO (ZnSO4.7H2O, Zn(CH3COO)2.2H2O, Zn (NO3)2.6H2O), respectively. The MIC showed the ZTO NPs for zinc salt ZnSO4.7H2O in Volumetric dilution 3.125% and 12.5 was more effective against Escherichia coli and Staphylococcus aureus from another zinc salt of ZTO NPs. Inhibition Zone assessed ZTO NPs' antibacterial efficacy against Staphylococcus aureus and Escherichia coli. With three concentrations of ZTO, the test showed that ZTO NPs have a stronger antibacterial activity against gram-positive bacteria than gram-negative bacteria. The antibacterial activity of zinc salt ZnSO4.7H2O was found to be 32 against Escherichia coli and 19 nm against Staphylococcus aurous, it was more effective than other zinc salt of ZTO NPs.

DETERMINATION OF TANNIN CONTENT AND ANTIBACTERIAL ACTIVITY OF CHROMOLAENA ODORATA L.

Aims and objectives: To ascertain the antibacterial capability using TLC-bioautography and the tannin content using the UV-Vis spectrophotometric method in the ethanol extract of kopasanda (Chromolaena odorata L.) leaves. Methods: Tannins quantitatively utilizing the UV-Vis spectrophotometric technique using the folin ciocalteu reagent at a wavelength of up to 687 nm. Antibacterial potential against gastrointestinal infection-causing bacteria (Salmonella typhi, Vibrio cholerae, Escherichia coli, and Shigella dysentriae) is assessed using TLC-bioautography technique. Results: The tannin content of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract. Escherichia coli, Shigella dysentriae, Salmonella typhi, and Vibrio cholerae, respectively, create inhibitory zones in the antibacterial potential test that yields 7, 8, 8, and 7 stains. Tannins, flavonoids, alkaloids, and saponins are believed to be among the chemicals found in the TLC results obtained with stain spraying reagents 1, 3, 5, and 4, respectively. Conclusions: The average tannin concentration of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract, and it has antibacterial properties against germs that cause gastrointestinal illnesses. Peer Review History: Received 22 September 2024; Reviewed 3 November; Accepted 15 December; Available online 15 January 2025 Academic Editor: Dr. Amany Mohamed Alboghdadly, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10

Evaluation of Essential Oils as Natural Antibacterial Agents for Eggshell Sanitization and Quality Preservation

Eggs are considered a nutritionally complete food and an excellent source of protein. However, they are prone to bacterial contamination, which can affect their internal quality and safety. This study aimed to evaluate the effectiveness of essential oils (EOs) as natural sanitizers for eggshells to reduce the bacterial load and improve shelf life. Ten EOs—cinnamon, clove, lavender, peppermint, thyme, rosemary, green tea, mustard, basil, and marjoram—were tested for antibacterial activity against three Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis, and Listeria monocytogenes) and three Gram-negative bacteria (Salmonella Enteritidis, Pseudomonas aeruginosa, and Escherichia coli) using the well diffusion method. The most effective EOs were assessed in vapor form using a disc volatilization technique. A total of 288 eggs from a local farm in South Lebanon were treated with selected EOs and stored at room temperature for three weeks to evaluate microbial load and quality. The eggs were grouped into six categories: dry control, ultraviolet (UV) treated, and eggs sprayed with EOs, based on the recorded minimum inhibitory concentration (MIC) values. At each time point, 12 eggs from each group were analyzed for microbiological testing and external and internal quality. Results indicated that nine of the ten EOs showed antibacterial activity, with the highest inhibition zones recorded for cinnamon against S. Enteritidis (14 mm) and E. coli (9 mm), peppermint against P. aeruginosa (10 mm), and rosemary against L. monocytogenes (22 mm), S. aureus (19 mm), and E. faecalis (12 mm). Vapor phase testing showed that rosemary, thyme, clove, and cinnamon EOs had average inhibition zones ranging from 21 to 33 mm against the tested bacteria, and peppermint EO achieved complete inhibition of P. aeruginosa. Clove EO consistently reduced the bacterial load as a sanitizer on eggs, initially achieving complete inhibition. While egg quality parameters such as weight, shell thickness, Haugh unit, and yolk color declined over time, EOs helped maintain better quality compared to dry controls. GC-MS analysis of the tested EOs revealed that monoterpene alcohols are the major constituents of most oils. This study concludes that EOs are effective natural sanitizers for eggs, enhancing safety and preserving quality during storage.

Investigation of the antimicrobial properties of temperature-sensitive hydrogel containing silver sulfadiazine against various bacterial strains

In burn wound management, infection poses a significant challenge, accounting for 75% of deaths in burn patients. Silver sulfadiazine is broadly used as an effective antibacterial agent for treating burns. Numerous researchers have explored various dosage forms of silver sulfadiazine, such as cream, ointment, topical spray, and hydrogel, for antimicrobial topical applications. Hydrogels offer appealing advantages over conventional drug delivery systems due to their sensitivity and responsiveness to stimuli, particularly temperature. Nevertheless, the comprehensive investigation of the antimicrobial properties of temperature-sensitive hydrogel containing silver sulfadiazine against different bacterial strains remains lacking. Thus, the main objective of the current study is to explore the antimicrobial properties of the temperature-sensitive hydrogel, incorporating silver sulfadiazine, against various bacterial strains colonized in burn wounds. To assess the antimicrobial activity of the temperature-sensitive hydrogel, inhibition zone diameters were measured against different types of Gram-positive strains (Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Streptococcus pyogenes) and Gram-negative strains (Escherichia coli and Klebsiella pneumoniae). The synthesized silver sulfadiazine-loaded temperature-sensitive hydrogel exhibited remarkable antimicrobial efficacy against these bacteria. Notably, there was no significant difference in the inhibition zone diameter between the silver sulfadiazine-loaded temperature-sensitive hydrogel and the positive control (p>0.05). These findings affirm that the silver sulfadiazine-loaded temperature-sensitive hydrogel holds promise as a drug delivery medium, demonstrating excellent antimicrobial activity against various bacterial strains that colonized in burn wounds.

Nutrient evaluation, minerals quantification and antibacterial potential of <i>Mentha longifolia</i> (L.) flower, leaves and stem against foodborne bacterial pathogens

This study was conducted for the assessment of the nutrition, minerals and antibacterial activities of leaves, stem and flower of Mentha longifolia (wild mint). Proximate compositions were carried out using standards methods, mineral analysis were determined using Atomic Absorption Spectrophotometer and Flame Photometer, antibacterial activities were assessed using Zone of Inhibition Assay. Broth dilution method was used to determine the Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The proximate composition of Mentha longifolia observed that moisture was found in the range of 2.3-2.8%, ash was 2.5-7.5%, crude fat was 0.5-2.1%, crude fiber was 8.5-22.5%, protein was 2.1-4.5%, carbohydrate was 69.6-78.7% and energy was 291.3-347.7 Kcal/100 g dw. Sodium concentration was found in the range of 1250-1590 mg/kg, Potassium was 3240-4820 mg/kg, Calcium was 12400-24000 mg/kg, Magnesium was 4800-9600 mg/kg, Zinc was 32-64 mg/kg, Iron was 140-498 mg/kg, Manganese was 32-107 mg/kg and Chromium was 3-6 mg/kg in Mentha longifolia leaves, flower and stem. The highest zone of inhibition (23±0.2) of Mentha longifolia leaves extract (tincture) was calculated against Bacillus cereus. Similarly the highest zone of inhibition of Mentha longifolia flower and stem was noted against Bacillus cereus in tincture extract were 17±01 mm and 16±01 mm respectively. The Mentha longifolia leaves MIC range was 40–120 mg/mL and MBC 60–200 mg/mL. The flower extracts MIC range 70–220 mg/mL and MBC 110–300 mg/mL. Generally the antibacterial activities of wild mint leaves extracts were higher as compared to flower and stem extracts (p<0.05). The results commonly observed Gram-negative bacteria are typically high opposed to the Mentha longifolia extracts as compared to Gram-positive bacteria. The proximate and minerals analysis concludes that this herb is used to formulate nutraceutical products and their extracts can be used against multi drug resistance bacteria accomplished of causing foodborne diseases.

Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts.

Terminalia schimperianaHochst, belonging to theCombretaceaefamily, is known for its ethnomedicinal values, particularly in treating various diseases in Africa. This study aimed to investigate the antibacterial, antioxidant, and cytotoxic properties of T. schimperiana stem bark extracts, with a specific focus on assessing their bioactive potential and identifying active compounds via TLC bioautography. The invitro antimicrobial activity was assessed using the agar well diffusion method against selected clinical strains. Antioxidant activity was evaluated using several methods including free radical scavenging, ferrous ion chelation assays and total phenolic content analysis. The cytotoxicity of the extracts was assessed using MTT assay towards Raw 264.7 and Vero cell lines. All extracts demonstrated significant antibacterial activity against the bacteria tested, with inhibition zones (IZ) ranging from 6.50±0.71 to 15.50±0.71 mm and minimum inhibitory concentrations (MIC) ranging from 1.95 to 1,250 μg/ml. The hydroethanolic extract exhibited strongest antioxidant activities with EC50 values of 188.50; 245.30, and 281.50 μg/mL for DPPH; ABTS, ferrous ion chelation assays respectively, and a high content of phenolic compounds (101.67±2.97 µgEFA/mg DW). Importantly, no cytotoxic effects were observed on Raw 264.7 and Vero cell lines. HPTLC analysis identified alkaloids and phenolic compounds in both aqueous and hydroethanolic extracts. These findings indicate T.schimperiana provides a wealth of bio-compounds that can be utilised in the pharmaceutical industry as antibacterial and antioxidant agents to combat antibiotic resistance.