Zn Concentration In Rice Research Articles

Variation of grain zinc, phytate concentration and phytate : Zn molar ratio in unpolished and polished rice affected by foliar zinc application among Thai rice varieties

ABSTRACT This study evaluated the variation in grain yield and Zn concentration in rice after foliar application of Zn and the impact on its phytate : Zn molar ratio among 21 rice varieties. Rice varieties were grown under field conditions using a randomized complete block design with four replications over two cropping years in 2020 and 2021 during the rainy season. Foliar Zn application varied grain yield from −26.0 to 13.6% in 2020 and −14.7 to 30.4% in 2021 compared with non-foliar Zn. Grain Zn was varied from 0.5 to 31.1% in 2020 and from −5.9 to 47.8% in 2021 in unpolished rice and from 9.4 to 47.0% in 2020 and from 8.2 to 163.0% in 2021 in polished rice. The phytate : Zn molar ratio was decreased when foliar Zn was applied to the unpolished and polished rice, with a lower ratio in the polished rice. The phytate : Zn molar ratio decreased exponentially with increasing grain Zn concentration in the unpolished rice, while decreasing linearly with increasing grain Zn concentration in the polished rice. The results of this study suggest that foliar Zn application is a promising way to improve the grain Zn concentration and decrease the phytate : Zn molar ratio, thus being beneficial for human diets, but the effects of rice variety, cropping season, and the polishing process should be considered.

Soil micronutrients (Zn and Fe) fractions and response of rice (Oryza Sativa) in different soil of Haryana under rice-wheat cropping system

The significance of zinc and iron in crop production, particularly in rice-wheat cropping systems, has been increasingly recognized. The present study aimed to determine the effect of different soil samples collected from the rice-wheat cropping system of Haryana with different soil texture on rice grain yield and micronutrient content using a greenhouse trial with four levels of Fe (0, 25, 50 mg kg−1 as soil and 0.5% foliar spray of FeSO4 at 45 DAS) and four levels of Zn (0, 5, 10 mg kg−1 as soil and 0.5% foliar spray of ZnSO4 at 35 DAS). This study discovered that rice grain yield showed a positive correlation with soil Zn and Fe concentrations in soil before sowing and a negative correlation with soil Fe and Zn concentrations in soil after crop harvesting. In most of the soils studied, foliar spray alone Fe @ 0.5% foliar (45 DAS) and Zn @ 0.5% foliar (35 DAS) improved Fe and Zn concentrations in rice significantly more than soil application of 25 mg Fe kg−1 and 5 mg Zn kg−1, respectively. The rice grain yield in clay soil was 14-42% higher than in sandy soil with different doses of Zn and Fe application. The order of preponderance of different Fe and Zn fractions were CA-Fe < OM-Fe < EX-Fe < FeMnOX-Fe < Res-Fe and Res-Zn > FeMnOX-Zn > OM-Zn > CA-Zn > Ex-Zn in the soils, respectively. Conclusively, concomitant consideration of grain yield and grain Zn and Fe concentrations of rice are the sustainable approach toward food targets achievement.

Bioaccumulation of zinc in rice (oryza sativa l.) from River Swat, Panjkora, And Kabul Pakistan

Ecosystem contamination with heavy metals lead to the bioaccumulation of these elements in crops. Rice, a significant component of the human diet, can bioaccumulate heavy metals and is of public health concerns. Therefore, the current research aimed to investigate the bioaccumulation of the heavy metal zinc in several rice varieties from three rivers in Pakistan's Malakand Division. The Highest Zn concentration of 91.58± 6.25 mg kg−1 dry weight was observed in rice roots of China Basmati variety grown across River Swat, while the highest Zn concentration of 60.06 ± 4.41 mg kg−1 dry weight was observed in the stem of Mota Chawal on River Kabul. In a site-wise comparison of both River Swat and Kabul, the Zn concentrations in the stem of Sara Sela rice on River Panjkora were 55.5±4.37, showing significant differences among different sites on the rivers. Similarly, comparing metal concentrations in different rice varieties generally did not show significant differences between the varieties. Zn concentrations in rice were higher in the Swat River than in the rest in all cases. Bioaccumulation factor (BAF) values for Zn were in the order of soil &gt; roots &gt; stem &gt; leaves &gt; grains for River Swat and Kabul. In contrast, for River Panjkora Zn concentration, the order was soil &gt; stem &gt; root &gt; grains&gt; leaves, respectively. Zn absorption by rice in the form of ions or particles generally affects plant phenotypic, physiological, and molecular development; hence, it must be considered in present varieties for the future.

Factors Affecting Dietary Intake of Copper and Zinc via Rice Consumption by Residents of Major Rice-Producing Regions in China

Background: Copper (Cu) and zinc (Zn) are essential nutrients that must be maintained at adequate levels in the human body in order to make physiological functions normal and sustainable. Rice is a leading staple cereal crop which can be the main source of Cu and Zn in the diet. Results: Here, we aimed to investigate Cu and Zn concentrations in rice with corresponding soil influencing factors and to assess the dietary intake of Cu and Zn from rice consumption by residents of major rice producing regions. A total of 712 rice grain and 90 paired soil–rice samples were collected from September to November 2015 covering eleven provinces across China. Average Cu and Zn concentrations were 27.2 and 69.1 mg kg−1, respectively, in soils, and 1.98 and 12.3 mg kg−1 in rice. The concentrations and bioconcentration factors (BCFs) of Cu and Zn followed the sequence: roots &gt; shoots &gt; grains. Soil pH, cation exchange capacity (CEC), and soil organic carbon (SOC) play important roles in rice Cu and Zn uptake with negative effects. The average Cu and Zn intakes from rice consumption were 0.597 and 3.68 mg day−1, respectively. Conclusions: The status of Cu and Zn daily intake from rice consumption were fully adequate but there remained some sampling sites where Zn intake for males and Cu intakes were deficient at 1.40%, indicating that local residents need to maintain their dietary nutrient requirements.

The potential of irradiated inoculants consortium for zinc accumulation in rice mutant lines

Some of the microorganisms in soil play important roles in plant performance by improving mineral nutrition. Zinc (Zn) is an essential micronutrient that has various important functions and is only obtained by the intake of foods. Rice is a staple food for Indonesian people. Increasing Zn concentration in rice through fertilization and gamma irradiated inoculants consortium is an effective way to accumulate Zn in rice grains. The objective of this research was to utilize gamma-irradiated inoculants and enhancement of Zn content in rice mutant lines. This study used inoculants consortium from potential microorganisms consisting of Aspergillus niger, Azotobacter sp, Bacillus circulants, and Trichoderma harzianum. They can be used to solubilize metals or minerals that are accumulated in part of the plants. The irradiation of the inoculant was conducted by 250 Gy gamma rays. Seven Mira-1 rice mutant lines together with their wild type, Inpari Nutri Zinc rice variety, were used. Treatment combinations, i.e., Control, T1, T2, T3, T4, and T5 were applied to the rice materials randomized block experimental design with three replications. Agronomic characteristics at the vegetative phase were observed and Zn content in rice grain was measured by atomic absorption spectrophotometry. The result shows significant differences in agronomic characteristics from different treatments. Zn content in rice mutant lines was higher than their wild type, and the irradiated inoculants consortium was very effective in enhancing Zn content in rice grains. The best treatment for accumulation of Zn in rice grain was irradiated inoculants consortium combined with 10 kg/ha zinc sulfate (treatment T4), resulting in a grain Zn content of 72.19 ppm in the 82 rice mutant line.

Genetic diversity studies for grain iron and zinc content analysis for Elite rice (Oryza sativa L.) genotype by using SSR markers

Rice is an important staple food, yet the polished rice lacks essential micronutrients. Biofortification strategy enhance the micronutrients in food crop. The current study used fourteen polished rice genotypes to assess genetic diversity and to determine the Fe and Zn value by Di-Acid Wet Digestion technique. The results highlighted that Kissan Basmati had a high iron content 44.65 ppm, whereas Super Basmati 2019 had the highest zinc value of 34.51 ppm. The correlation between the marker and traits were determined using single marker analysis. For the analysis, ten SSR makers were utilized, with eight of them yielding polymorphic data. Monomorphic results were found for RM211 and RM331. Two markers, RM430 and RM437, were found to have a P-value of less than 0.05. RM430 had a P-value of 0.033 for zinc, whereas RM437 had a P-value of 0.046 for iron and 0.001 for zinc, with R2 values of 46.07, 23.46, and 45.07, respectively. This meant that RM430 had a statistically significant relationship with zinc content and RM437 had a statistically significant relationship with both micronutrients (Fe and Zn). These markers could be utilized as a technique to determine the Fe and Zn concentrations in rice. Consequently, the identified genotypes with improved Fe and Zn content could be utilized as donor in the future breeding program. In summary, our research shows that SSR technology is an effective method for assessing genomic diversity.

Agronomic Biofortification of Zinc in Rice for Diminishing Malnutrition in South Asia

Zinc (Zn) is increasingly recognized as an essential trace element in the human diet that mediates a plethora of health conditions, including immune responses to infectious diseases. Interestingly, the geographical distribution of human dietary Zn deficiency overlaps with soil Zn deficiency. In South Asia, Zn malnutrition is high due to excessive consumption of rice with low Zn content. Interventions such as dietary diversification, food fortification, supplementation, and biofortification are followed to address Zn malnutrition. Among these, Zn biofortification of rice is the most encouraging, cost-effective, and sustainable for South Asia. Biofortification through conventional breeding and transgenic approaches has been achieved in cereals; however, if the soil is deficient in Zn, then these approaches are not advantageous. Therefore, in this article, we review strategies for enhancing the Zn concentration of rice through agronomic biofortification such as timing, dose, and method of Zn fertilizer application, and how nitrogen and phosphorus application as well as crop establishment methods influence Zn concentration in rice. We also propose data-driven Zn recommendations to anticipate crop responses to Zn fertilization and targeted policies that support agronomic biofortification in regions where crop responses to Zn fertilizer are high.

Genomic prediction of zinc-biofortification potential in rice gene bank accessions

Key messageA genomic prediction model successfully predicted grain Zn concentrations in 3000 gene bank accessions and this was verified experimentally with selected potential donors having high on-farm grain-Zn in Madagascar.Increasing zinc (Zn) concentrations in edible parts of food crops, an approach termed Zn-biofortification, is a global breeding objective to alleviate micro-nutrient malnutrition. In particular, infants in countries like Madagascar are at risk of Zn deficiency because their dominant food source, rice, contains insufficient Zn. Biofortified rice varieties with increased grain Zn concentrations would offer a solution and our objective is to explore the genotypic variation present among rice gene bank accessions and to possibly identify underlying genetic factors through genomic prediction and genome-wide association studies (GWAS). A training set of 253 rice accessions was grown at two field sites in Madagascar to determine grain Zn concentrations and grain yield. A multi-locus GWAS analysis identified eight loci. Among these, QTN_11.3 had the largest effect and a rare allele increased grain Zn concentrations by 15%. A genomic prediction model was developed from the above training set to predict Zn concentrations of 3000 sequenced rice accessions. Predicted concentrations ranged from 17.1 to 40.2 ppm with a prediction accuracy of 0.51. An independent confirmation with 61 gene bank seed samples provided high correlations (r = 0.74) between measured and predicted values. Accessions from the aus sub-species had the highest predicted grain Zn concentrations and these were confirmed in additional field experiments, with one potential donor having more than twice the grain Zn compared to a local check variety. We conclude utilizing donors from the aus sub-species and employing genomic selection during the breeding process is the most promising approach to raise grain Zn concentrations in rice.

Improving Grain Zinc Concentration in Wetland and Upland Rice Varieties Grown under Waterlogged and Well-Drained Soils by Applying Zinc Fertilizer

The objective of this study was to evaluate the responses in grain yield and zinc concentration of wetland and upland rice varieties to Zn fertilizer application and different growing conditions. The wetland (Chainat 1; CNT1) and upland (Kum Hom CMU; KH CMU) rice varieties were grown under waterlogged and well-drained soil conditions with or without Zn fertilizer application. Zinc fertilizer (ZnSO4) was applied at 0 and 60 kg ha−1 in three stages at tillering, booting, and flowering. In the wetland variety, CNT1, grain yield decreased by 18.0% in the well-drained soil compared to the waterlogged conditions, but there was an 8.9% decrease in grain yield in the waterlogged soil compared to the well-drained soil in the upland variety, KH CMU. Applying Zn fertilizer affected yields differently between the varieties, decreasing grain yield by 11.9% in CNT1 while having no effect in KH CMU. For grain Zn concentrations in brown rice, applying Zn fertilizer increased Zn concentration by 16.5–23.1% in CNT1 and KH CMU under both growing conditions. In the well-drained soil, applying Zn fertilizer increased straw Zn concentration by 51.6% in CNT1 and by 43.4% in KH CMU compared with the waterlogged conditions. These results indicated that the wetland and upland rice varieties responded differently to Zn fertilizer application when grown in different conditions. Applying Zn fertilizer in the appropriate rice variety and growing conditions would help farmers to improve both the desirable grain yield and Zn concentration in rice.

Ghd7 is a negative regulator of zinc concentration in brown rice

Zinc (Zn) is an essential micronutrient for human, and improving Zn concentration in rice could be a simple and effective way to solve insufficient Zn uptake from daily diet. In this study, a recombinant inbred line (RIL) population was developed from a commercial hybrid combination Wandao153, and subjected to quantitative trait loci (QTL) detection for Zn concentration in brown rice and heading date. Five QTL for Zn concentration and six for heading date were detected across 2 years, among which two QTL for Zn concentration and two for heading date were co-located on chromosome 7 and 8, respectively. The two co-located QTL on chromosome 7, qZn7 and qHd7, were likely to be Ghd7, a major gene for heading date and yield potential in rice. A combination of Zn concentration analysis and haplotype analysis of three pairs of genetic materials for Ghd7 revealed that it is a negative regulator of Zn concentration in brown rice. The other two co-located QTL on chromosome 8, qZn8 and qHd8, were likely to be Ghd8/DTH8, another major gene for heading date and yield potential in rice. These results could be of great use in genetic improvement of Zn concentration in brown rice.

Effects of zinc oxide nanoparticles on arsenic stress in rice (Oryza sativa L.): germination, early growth, and arsenic uptake.

This study describes the role of zinc oxide nanoparticles (ZnO NPs) in alleviating arsenic (As) stress in rice (Oryza sativa) germination and early seedling growth. Seeds of rice were primed with different concentrations (10, 20, 50, 100, and 200mgL-1) of ZnO NPs and As (0, and 2mgL-1) for 12days in petri dishes. Two milligrams per liter of As treatment represented a stress condition, which was evidenced by germination rate, seedling length, seedling dry weight, chlorophyll, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) content of rice shoot. ZnO NPs amendment (10-100mgL-1) increased the germination rate (2.3-8.9%), shoot weight (18.2-42.4%), root weight (5.2-23.9%), and chlorophyll content (3.5-40.1%), while elevated the SOD (2.2-22.8%) and CAT (7.2-60.7%) activities and reduced the MDA content (17.5-30.8%). As concentrations were significantly decreased by 8.4-72.3% and 10.2-56.6%, respectively, in rice roots and shoots with ZnO NPs amendment (10-200mgL-1) by the As adsorption of ZnO NPs and promoted biomass of rice. All the amendments improved the Zn concentrations in rice shoots and roots. Overall, ZnO NPs provide effective resistance to arsenic toxicity by increasing germination, biomass, and nutrients of Zn and decreasing As uptake in rice.

Zinc Concentration in Rice (Oryza sativa L.) Grains and Allocation in Plants as Affected by Different Zinc Fertilization Strategies

ABSTRACTConcern over the food chain transfer of zinc (Zn) is increasing because of its importance in human health. A field experiment was conducted on a low Zn soil to determine the effect of different Zn fertilization strategies on grain Zn concentration and Zn allocation in different plant tissues of rice. Six treatments were used: (1) no Zn fertilization; (2) soil fertilization at transplanting; (3) Zn soil fertilization at transplanting and flowering; (4) foliar application during grain filling; (5) foliar applications during tillering, flowering, and grain filling; and (6) combination of treatments 3 and 5. Zn fertilization significantly increased Zn concentration in brown rice. The largest effect on grain Zn was observed by combination of soil and foliar applications. The increase in brown rice was much smaller (20%) than the increase in the vegetative parts (100%), indicating that grain Zn concentration of rice is not strongly increased by Zn fertilization. More increased Zn by Zn fertilization was allocated into straw not into grain. From the perspective of human nutrition, it seems that there is too little scope to enhance Zn concentration in rice by fertilization alone. the major bottleneck to increase Zn concentration in rice grain seems to be internal translocation/retranslocation of Zn from shoot to panicle or from rachis to grain, rather than root uptake of Zn from the soil.

Effects of Application of Different Sources of Zn and Composts on Zn Concentration and Uptake by Upland Rice

Global efforts are under way to improve the Zn concentrations in rice to increase Zn in human diets. Therefore, this pot experiment was conducted to examine the effects of Zn sources and different composts on two upland rice varieties. This study was a 3 factors experiment with 3 replications arrange in RCBD. Experimental units include: 2 selected upland rice varieties with highest and lowest Zn uptake, 12 treatments, including 3 types of composts (oil palm compost, vermicompost and poultry compost) and 2 different sources of Zn (ZnS04 and Zn-EDTA). Soil samples were analyzed before and after harvest. The plants were harvested 16-20 weeks after planting and analysed. All types of composts showed positive effects on Zn concentration and uptake in all parts of rice. Vermicompost is the most effective compost among these three composts. Both inorganic Zn sources used showed almost the same Zn concentration and uptake by rice in this experiment. The magnitude of Zn uptake response was magnified when zinc sulphate was applied along with organic compost. Application of Zn-amended organic composts increased the percentage distribution of Zn in grain more than the application of ZnSO4 or Zn-EDTA alone.

Upland Rice Root Parameters and Their Relationship on Utilizing Different Levels of Applied Zinc

Global efforts are under way to improve the Zn concentrations in rice to increase Zn in diets. Zinc uptake in relation to morphological root parameters among 7 upland rice varieties was studied by conducting a solution culture experiment using modified Yoshida solution in Agriculture Faculty of University Putra Malaysia. Five zinc levels were developed by the addition of 0, 5, 10, 20, 30 mgL -1 ZnSO4. Seedlings were harvested in week 4. Zn uptake in roots of upland rice showed significant differences among all varieties. Zn uptake significantly increased at all rates. Other root parameters (length, average diameter, surface area, volume, and number of root tips) did not show any significant differences in 0 to 20 mg ZnL -1 , but they decreased significantly in 30 mg ZnL -1 in 4th week of observation.

Contrasting grain-Zn response of biofortification rice (Oryza sativa L.) breeding lines to foliar Zn application

Enriching grain Zn concentration in rice through Zn fertilization is one approach to Zn biofortification efforts. Experiments to understand the effectiveness of foliar Zn application at different growth stages in enriching grain Zn of biofortification breeding lines were conducted during the dry season at an experiment station and in a farmer's field with moderately and severely Zn-deficient soils, respectively. Foliar Zn applications at 4kgZnha−1 as zinc sulfate were given at different growth stages: active tillering, heading, and both active tillering+heading. Foliar Zn application at active tillering had no effect on brown rice Zn concentration, but, at heading, there was a range in genotype response from 1 to 10mgkg−1 increase, with an average increase of 4mgkg−1 at the moderately Zn-deficient site and 7mgkg−1 at the severely Zn-deficient site. At the moderately Zn-deficient site, two biofortification breeding lines (IR83668, IR91152AC) reached the target of 30mgkg−1 Zn in brown rice without Zn fertilization at heading stage, while two other genotypes (IR68144, IR91143AC) reached the target with heading-stage foliar Zn application. At the severely Zn-deficient site, only one of the tested genotypes (IR68144) reached the target Zn concentration even after foliar Zn application at both active tillering and heading stage and the same genotype recorded the greatest response to foliar Zn (10mgkg−1 increase). Greater total leaf area at spraying time increased the effectiveness of foliar Zn application at the severely Zn-deficient site only. Foliar Zn application at the two tested growth stages failed to overcome agronomic Zn deficiency. Three of the biofortification breeding lines (IR68144, IR85800, and IR83668) had high grain Zn content that was independent of grain yield. In a separate experiment to test a wider range of spraying times at the severely deficient site with IR64, the spraying at the early milk stage emerged as the most effective stage for increasing brown rice Zn concentration. Our results show that agronomic Zn biofortification through foliar Zn application is likely to be much more effective at increasing grain Zn concentration of genotypes with strong Zn-remobilization capacity than those with weak remobilization capacity.

Effects of Different Cultivation Methods and Straw Incorporation on Grain Yield and Nutrition Quality of Rice

Plastic film mulching cultivation (PFMC) under non-flooded condition has been considered as a new water-saving technique in rice production, while yield decline from continuous cropping of aerobic rice is a constraint to the widespread adoption of PFMC. Rice straw incorporation has been proposed to counter this negative effect in recent decades. This study examined the effects of three cultivation methods and rice straw incorporation on rice grain yield and quality using Liangyoupeijiu (an indica hybrid). The three cultivation treatments were: conventional flooding cultivation (CFC); non-flooded plastic film mulching cultivation (PFMC); no mulching cultivation in non-flooded condition (NMC). Compared with that under CFC, average rice grain yield under PFMC from 2008 to 2010 was significantly improved by 8.0%, while total amino acids content was decreased by 3.5%, no obvious effect was observed on rice protein, Fe and Zn concentrations in rice; under NMC, the reduction in yield, protein, total amino acids and Fe concentrations were 5.1%, 4.4%, 9.3%, and 11.8%, respectively. With rice straw incorporation, grain yield was improved by 113.6, 142.6, and 522.1 kg ha 1 under CFC, NMC, and PFMC, respectively. Average rice grain yield, Fe and Zn contents in brown rice were significantly improved by 3.3%, 3.1%, and 6.4% with rice straw incorporation. The results of the three years showed the same trend in rice grain yield and nutrition quality. The results indicated that PFMC could improve not only grain yield, but also part of rice nutrition quality. Rice straw incorporation could significantly improved rice grain yield as well as Fe and Zn concentrations in brown rice, which would be a good method to overcome grain yield decline under long term PFMC.

Environmental contamination and seasonal variation of metals in soils, plants and waters in the paddy fields around a PbZn mine in Korea

The objective of this study is to investigate the extent and degree of heavy metal contamination of paddy fields influenced by metalliferous mining activity. Paddy soils, rice plants and irrigation waters were sampled along six traverse lines in the vicinity of the mine and nearby control site. Soil samples were taken 30, 80 and 150 days after rice transplanting, to study seasonal variation of their chemical properties and heavy metal concentrations. Sampling of rice plants and irrigation waters was also undertaken with seasons. The analysis of the samples were carried out using ICP-AES for 25 elements including Cd, Cu, Pb and Zn. Physical and chemical properties of soils (pH, loss-on-ignition, cation exchange capacity and texture) and waters (pH, Eh and temperature) were also measured. The properties of soils were similar to the average Korean soils, with the exception of some samples taken in the vicinity of the mine. Concentrations of Cd, Cu, Pb and Zn in paddy soils, rice plants and irrigation waters sampled in the immediate vicinity of the mine were relatively high due to the seepage of metals from mining dump sites. Although there was variation between sampling sites, soil pH values under reducing conditions were on average higher than those under oxidising conditions. Relatively low content of organic matter and low cation exchange capacity of soils were found at 80 days after rice transplanting ( P < 0.05). No seasonal variations in metal concentrations were found in paddy soils throughout the period of the rice growing, in which soils ranged from flooded reducing conditions through most of the growing season to drained oxidising conditions before and at harvest. Relatively high metal concentrations were found in the rice stalks and leaves under oxidising conditions. The sequential extraction analysis of selected soil samples confirmed that high proportions of exchangeable fractions of the metals were found under oxidising conditions. It was shown that Cd and Zn concentrations in rice leaves and stalks and rice grain increased with increasing metal concentrations in paddy soils to a greater extent than for Cu and Pb. This difference in uptake is in agreement with the greater proportions of Cd and Zn, compared with Cu and Pb, in the exchangeable soil fraction extracted with MgCl 2. Average daily intake from locally grown rice by the residents was estimated to be 121 μg Cd and 126 μg Pb. Thus, long-term metal exposure by regular consumption of the rice poses potential health problems to residents in the vicinity of the mine, although no adverse health effects have as yet been observed.

Effects of copper toxicity on growth and the uptake and translocation of metals in rice plants

Abstract Copper (Cu) contamination in the lower reaches of the Sado River in the Alcacer do Sal region of Portugal (a major rice producing area) has became a major pollution problem. In an attempt to study the changes in rice growth (Oryza sativa L.), the effect of excess Cu on the plasma membrane activity (as measured throughout proton extrusion) and membrane permeability as well as on the zinc (Zn), nitrogen (N), phosphorus (P), potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), boron (B), molybdenum (Mo), and aluminum (Al) net uptake and translocation was studied. Long‐term studies (30 days) were conducted with rice subjected to increasing Cu concentrations (ranging from 0.002 to 6.25 mg/L) showed a concentration increase of Cu in root and shoot tissues. Root proton extrusion increased 7.4‐fold between the 0.01 and the 1.25 mg/L Cu treatments, whereas its membrane permeability (as measured throughout the electrolytic conductance) revealed a marked increase after the 1.25 mg/L Cu treatment. Zinc concentrations decreased with increasing Cu levels in the nutrient solution (excepting the 6.25 mg/L Cu treatment in shoots), while N, P, K, Na, Ca, Mg, B, Mo, and Al concentrations, although showing different patterns, did not reveal any correlation with increasing Cu level. The absolute content of all these metals as well as their net uptake exhibited (excepting Al) its highest values in the 0.25 mg/L Cu treatment. It is suggested that these variations, triggered by excess Cu on root growth, might be explained based on the interaction among Cu‐uptake mechanism(s), plasma membrane‐H+ ATPase and root membrane permeability. It is also suggested that Cu affects the N, P, K, Na, Ca, Mg, B, Mo, and Zn concentrations in rice shoots mainly by changing their net uptake rate.