Particle Size Of Zinc Oxide Research Articles

Elastomeric Biocomposites of Natural Rubber Containing Biosynthesized Zinc Oxide

Zinc oxide (ZnO) particles were successfully synthesized through the green method using aloe vera extract and zinc nitrate (1:1). The structure, morphology and properties of the biosynthesized ZnO (bioZnO) particles were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), time of flight secondary ion mass spectrometry (TOF-SIMS) and thermogravimetry (TG). The morphology and the size of ZnO particles were elucidated by scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS). Then, the ability of bioZnO to activate sulfur curing of natural rubber (NR) was tested and compared to commercial ZnO traditionally used as vulcanization activator. The bioZnO showed similar activity in the vulcanization process to commercial ZnO. NR composites containing bioZnO were pro-ecological in nature and exhibited better mechanical characteristics and durability against thermo-oxidative aging than NR with commonly used micrometric ZnO. Moreover, NR vulcanizates containing bioZnO showed good mechanical properties in dynamic conditions and satisfactory thermal stability. The present research is new and in addition to the analysis of biosynthesized ZnO particles, the effect of the activator in the vulcanization process of the NR elastomer and its influence on the properties of the final products were additionally discussed.

Superhydrophobic and antibacterial larch wood prepared by PEI controlled growth of conical ZnO nanopillar

The natural antibacterial performance of cicada wings inspired the morphology design and application of zinc oxide (ZnO) nanoparticles on wood surfaces to enhance combined antibacterial function. The commonly interior used larch wood was chosen as base material and firstly pre-treated with ultrasonic-hydrothermal method, the deposition of ZnO nanoparticles with different morphologies was controlled by polyethyleneimine (PEI) concentration during the crystal growth, and the analysis of surface characteristics and antibacterial efficacy was fully tested. Scanning Electron Microscopy (SEM) observations revealed that the addition of PEI altered the morphology of ZnO nanoparticles on the wood surface. ZnO structures without PEI exhibited hexagonal prismatic shapes. As the PEI concentration increased, the particle size of ZnO decreased. When 6 mmol/L of PEI was added, ZnO transformed into a conical structure. Contact angle measurements confirmed the preparation of superhydrophobic ZnO, which exhibited self-cleaning properties. The antibacterial circle method test results showed that superhydrophobic ZnO wood prepared with 6 mmol/L of PEI exhibited superior antibacterial properties, with higher activity against Staphylococcus aureus compared to Escherichia coli. Overall, the study demonstrates a promising approach to developing polyfunctional and effective antibacterial wood surfaces inspired by natural cicada wings nanostructures.

Effect of Calcination Temperature on Crystalline phase and Grain size of ZnO Particles

In today's technology, ZnO as a semiconductor, has emerged as a leading candidate in green environmental management systems, owing to its strong oxidation ability, good photocatalytic properties, and large free-exciton binding energy. ZnO is a costeffective, stable, and high photocatalytic material with good electrical properties and light transmittance, making it useful for various applications such as solar cells, photocatalysts, and electrical equipment. ZnO, being a photocatalyst, can be used for environmental clean-up efforts such as air purification, water purification, and deodorization. In this study, the zinc oxide (ZnO) was synthesised with a forced hydrolysis method and characterised by different characterization techniques. X-ray diffraction (XRD) was used to investigate the change in phase and crystal structure of ZnO particles at different calcination temperatures. Moreover, the average crystallite size of the particles was calculated at various calcination temperatures, using Scherrer equation. Also Scanning Electron Microscopy (SEM) used to image the surface morphology and size of the particles and it is found that the calcination temperature had a significant effect on the morphology of the particles. Additionally, differential Thermal Analysis (DTA) and Thermogravimetric Analysis (TGA) were used to observe the material transitions and changes in mass as the temperature increased.

The role of submicron zinc oxide particle size in improving UV protection by textiles

The role of submicron zinc oxide particle size in improving UV protection by textiles

Chitosan-photocatalyst nanocomposite on polyethylene films as antimicrobial coating for food packaging

Chitosan (CS), an edible and non-toxic natural biopolymer, has been widely used in food preservation attributed to its intrinsic antimicrobial, biodegradable, and excellent film-forming properties. In this work, we report photocatalyst-loaded chitosan coating on commercial polyethylene (PE) film with enhanced antimicrobial properties for food packaging application. To improve the chemical stability of zinc oxide (ZnO) photocatalyst in acidic chitosan matrix, a thin layer (1–5 nm) of amorphous tin oxide (SnOx) was coated on ZnO nanoparticles. Consequently, the charge transfer efficiency of ZnO is improved and most of the surface defects are retained according to the studies of UV–Vis and fluorescence spectroscopy. The thin SnOx coating on ZnO was observed by high-resolution transmission electron microscopy (HRTEM) and its effects on crystallinity and particle size of ZnO were examined using X-ray diffraction (XRD) and particle sizer, respectively. The addition of ZnO@SnOx particles in chitosan coating increases water contact angle (WCA) and enhances thermal stability of chitosan coating. The antimicrobial activity of chitosan, ZnO-SnOx nanoparticles, and CS-ZnO@SnOx coated PE films were examined against both Gram-negative (E. coli, A. faecalis) and Gram-positive (S. aureus, B. subtilis) bacteria. Compared to the limited antimicrobial effects of chitosan, ZnO-SnOx demonstrates an improved inhibition effect on bacterial growth over 48 h period under light. For the CS-ZnO@SnOx nanocomposite coated PE films, no inhibition zone was observed due to the limitation of disc diffusion method. Meanwhile, there were no bacterial colonies found to develop on the film, rendering this CS nanocomposite coating a good candidate for food packaging applications.

Investigating the size-dependent structural, optical, dielectric, and photocatalytic properties of benign-synthesized ZnO nanoparticles

Zinc oxide (ZnO) plays a vital role in numerous technological facets of semiconductors. Due to its captivating characteristics, it has garnered considerable interest in a diverse array of applications. In this study, the egg albumen-facilitated bio-template approach was utilized to fabricate ZnO nanoparticles, aiming to investigate the impact of varying albumen concentration ratios on the crystal structure, dimensions, and shape of the resulting ZnO nanoparticles. This approach provides more precise customization of the dimensions and configuration of the nanocrystals and is compatible with most documented physical techniques. Exploring the morphological impacts of unadulterated or altered zinc oxide nanocrystals on photocatalytic performance is crucial for maximizing solar energy absorption and utilization. However, this aspect has yet to receive much attention in previous studies. The photocatalytic rate was discovered to be independent of the ZnO particle size, but the geometry factor is of paramount significance. ZnO with a nanopencil shape exhibited a minimum of 1.81 times higher efficiency than spherical particles. Furthermore, the dielectric characteristics of ZnO nanoparticles (NPs) were investigated across a frequency range of 75 kHz to 7 MHz, considering the dependence on particle size. As the dimensions of the ZnO NPs diminish, there is a gradual rise in the dielectric permittivity, increasing from 20 to 82 with the escalation in frequency. In the context of ZnO nanoparticles (NPs), the Maxwell- Wagner polarization model can elucidate the phenomenon of robust interfacial polarization, particularly as the ZnO size diminishes. As the frequency increases, the AC conductivity exhibits a gradual augmentation. Likewise, in relation to the dielectric constant and losses, the electrical conductivity characteristics of all ZnO NPs samples exhibit a reliance on the size of the crystals, where a reduction in crystal size corresponds to a decrease in electrical conductivity. The utilization of eco-friendly nanomaterials is anticipated to pave the way for novel possibilities in their exceptional electronic applications as storage materials.

Effect of zinc oxide nanoparticle size on the dielectric properties of polypropylene‐based nanocomposites

AbstractTo develop new environmentally friendly and recyclable high‐voltage cable insulation materials, the effects of the particle size of zinc oxide (ZnO) on the mechanical and electrical properties of nanocomposites were investigated based on a feasibility study of polypropylene (PP) as a high‐performance cable insulation material. The small particle size of ZnO can synergistically toughen PP with a polyolefin elastomer (POE), but the large particle size of ZnO leads to lower elongation at break. Adding ZnO nanoparticles can improve the bulk resistivity of the composites and suppress space charge injection. 0.5ZnO200 has the highest direct current and alternating current breakdown field strengths, 36.6% and 14.7% higher than PP/POE, respectively. This improvement may be due to the ability of 200 nm ZnO to introduce more deep traps. However, as the nanoparticle content rises and the particle size decreases, the agglomeration becomes more frequent, leading to the overlap of inter‐nano interfaces and reducing the modification effect of the nanoparticles. This study on the effect law of nano‐ZnO with different particle sizes on the microstructure, mechanical properties, and electrical properties of PP can provide a reference for developing new recyclable high‐voltage insulated cable materials.

Assessment the Ag and ZnO Biosynthesized Nanoparticles effects on Giardia lamblia trophozoites which grown in HSP-1 culture media

Chemotherapy with drugs such as Metronidazole (MTZ) derivative products is currently the preferred treatment for giardiasis. However, these agents have been linked to a variety of negative side effects, varying from nausea to probable geno toxicity. Aim of this study to conduct an in vitro study on the efficiency of silver (Ag) and zinc oxide (ZnO) nanoparticles (NPs) biosynthesized by green ecofriendly method using Pseudomonas aeruginosa and Escherichia coli against Giardia trophozoites. Methods Silver and Zinc oxide NPs formation was confirmed based on ability of selected bacteria to biosynthesized these NPs. The particle size arranges of (29.5 nm) for Ag P. aeruginosa and (32 nm) for Ag E. coli. Particle size of ZnO for P. aeruginosa was (25 nm) and (29.7nm) for E. coli. Giardia trophozoites cultivated on HSP-1 media were subjected to different concentration of biosynthesized NPs at (0.025, 0.050, 0.075 mg/ml) for (24, 48, 72 hrs.). Significant reduction (P<0.05) in trophozoite number was recorded by the two groups of Ag and ZnO nanoparticles for both bacteria and was 91% for Ag NPs for both E. coli and P. aeruginosa and 73% for ZnO for both bacteria at concentration of 0.075mg/ml after 72 hrs. Morphological difference appeared as destructive with the release of the cytoplasm outside. The cytotoxic effects of Ag and ZnO NPs on Giardia lamblia trophozoite exceeded that of metronidazole, these particles can be recommended for use, especially at higher concentrations that lead to total death rates.

Investigation of the parameters affecting the morphology of zinc oxide (ZnO) nanoparticles synthesized by precipitation method

In this work, zinc oxide (ZnO) nanoparticles were synthesized using aqueous solutions of ZnSO4 and NaOH at ambient temperature and 70 °C. The nanoparticles synthesized at these two temperatures were identified and analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), infrared spectroscopy (FTIR), dynamic light scattering (DLS), and zeta potential (ZP). SEM micrographs showed that temperature, method of mixing, and stoichiometric ratio could significantly affect the formation of particles of different sizes and morphology. Phase analysis of synthesized particles was performed by X-ray diffraction. The result showed that temperature had the tremendous potential to convert precursors into products to form a single-phase compound completely. These results were confirmed by the observed characteristic peaks in FTIR. The crystallite size was calculated using Scherer's equation and Rietveld analysis to investigate the effect of the calcination process on the crystal size of the synthesized particles. The results showed that the calcination process could significantly affect the crystal size of the synthesized particles. Both methods showed an increase in the average crystal size of zinc oxide particles after the calcination process. The absolute magnitude of the zeta potential for zinc oxide particles with a pyramidal rod morphology with an average diameter of 80–90 nm was 33.2 and for star-like morphology with an average diameter of 800–900 nm was 11.8.

Post-deposition heat treatment effect on pH sensing behavior of chemical bath deposited nanostructured zinc oxide

The post-deposition heat treatment process was studied to improve zinc oxide (ZnO) ‘s pH sensing performance as the sensing electrode (SE) of extended-gate field effect transistor (EGFET) pH sensor. The low-temperature chemical bath deposited ZnO nanostructured thin film was heated from 200 to 400 °C before being applied as the SE. The heat treatment enhanced the agglomeration of ZnO nanoparticles, and the formation of nanonails was seen at the highest temperature of 400 °C. It was proven by XRD results that the crystallinity of ZnO samples were improved when the ZnO SE was heated at 300 °C and formed different growth orientations at (100), (002) and (101) before turning to (101) growth orientation at 400 °C heat treatment. The heat treatment process also increased the ZnO particle size from 15.47 nm to 260 nm at 300 °C. Further increasing the heat treatment up to 400 °C decreased the particle size to 44.30 nm. The ZnO morphology was correlated with the pH sensor performance. The clustered structure of the untreated ZnO sample exhibited a good pH sensitivity of 52.4 mV/pH with a linearity of 0.93 and a hysteresis value of 46.71 mV. On the other hand, almost similar pH sensitivity was obtained from the sample treated at 300 °C having poly-structure morphology, which was 52.9 mV/pH, but with a significantly improved linearity of 0.99, and hysteresis values of 41.99 mV respectively. The 300 °C heat treatment also improved the stability of ZnO SE performance. The difference in the performance of these two samples can also be related to the crystalline quality of the film.

Controlling the Surface Morphology of ZnO Nano-Thin Film Using the Spin Coating Technique.

Zinc oxide (ZnO) thin films are significant in various electronic applications. This study introduced an efficient, simple, low cost and timesaving method to obtain an extended and uniform ZnO thin film with tunable surface morphology over the substrate using the spin coating technique. Different concentrations of zinc acetate dehydrate were used as precursor solutions mixed with polyvinyl alcohol as a binding polymer to obtain the film’s uniformity and to relieve thermal expansion that may cause a wrinkled surface. Synthesized films were characterized using X-ray diffraction (XRD), X-ray spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and ellipsometry. Findings show that the average size of ZnO particles was less than 50 nm in a uniform film over the whole substrate area regardless of the presence or absence of wrinkles. Additionally, this method was quite fast, attaining the desired viscosity in less than one hour in comparison with the time-consuming aging method, which requires approximately 24 h to achieve the required viscosity.

Investigating the Antibacterial Properties of ZnO-NPs

The size range of several hundred nanometers to several of micrometers contains a lot of microorganisms. The aim of the current report was to study the antibacterial properties of ZnO-NPs through a theoretical review of the most important findings in previous studies. The study found the most important antibacterial properties of ZnO-NPs, including: Strong antibacterial results can be achieved by enhancing variables such as UV light, zinc oxide particle size, concentration, shape, and surface modification, which affect many different toxicological processes. One of the main properties of ZnO-NPs is the production of intracellular ROS formation, as well as the mechanical damage of the cell wall due to the release of Zn2+ ion and its attachment to the cell membrane.

Application of Response Surface Methodology for Optimization of Nanosized Zinc Oxide Synthesis Conditions by Electrospinning Technique.

Zinc oxide (ZnO) is a well-known semiconductor material due to its excellent electrical, mechanical, and unique optical properties. ZnO nanoparticles are widely used for the industrial-scale manufacture of microelectronic and optoelectronic devices, including metal oxide semiconductor (MOS) gas sensors, light-emitting diodes, transistors, capacitors, and solar cells. This study proposes optimization of synthesis parameters of nanosized ZnO by the electrospinning technique. A Box–Behnken design (BB) has been applied using response surface methodology (RSM) to optimize the selected electrospinning and sintering conditions. The effects of the applied voltage, tip-to-collector distance, and annealing temperature on the size of ZnO particles were successfully investigated. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images confirm the formation of polyvinylpyrrolidone-zinc acetate (PVP-ZnAc) fibers and nanostructured ZnO after annealing. X-ray diffraction (XRD) patterns indicate a pure phase of the hexagonal structure of ZnO with high crystallinity. Minimal-sized ZnO nanoparticles were synthesized at a constant applied potential of 16 kV, with a distance between collector and nozzle of 12 cm, flow rate of 1 mL/h, and calcination temperature of 600 °C. The results suggest that nanosized ZnO with precise control of size and morphology can be fabricated by varying electrospinning conditions, precursor solution concentration, and sintering temperature.

Synthesis of ZnO and CuO nanoparticles via Sol gel method and its characterization by using various technique

Nanotechnology is a completely unique branch of technology that offers with substances in a very small size between (1–100 nm) with various crystal shapes. Metals have ability to produce large number of oxides. These metal oxides play a major role in many areas of chemistry, physics, material science and food science. In this research, Zinc Oxide (ZnO) and Copper (II) oxide nanoparticles were synthesized via sol–gel process using zinc nitrate and copper (II) nitrate as precursor respectively. The characterization of CuO and ZnO nanoparticles was done by using various techniques. X-ray Diffraction (XRD) indicates the crystallinity and crystal size of CuO and ZnO nanoparticle. Fourier transform infrared spectroscopy (FT-IR) was used to get the infrared spectrum of the sample indicating composition of the sample which contains various functional groups. XRD result shows the particle size of CuO at highest peak 29.40140 was 61.25 nm and the particle size of ZnO at highest peak 36.2476° was 21.82 nm. FT-IR spectra peak at 594.56 cm-1 indicated characteristic absorption bands of ZnO nanoparticles and the broad band peak at 3506.9 cm−1 can be attributed to the characteristic absorption of O–H group. The analysis of FT-IR spectrum of CuO shows peaks at 602.09, 678.39, and 730.19 cm−1 which refer to the formation of CuO. SEMimages indicate the morphology of CuO and ZnO nanoparticles. Result of EDX characterization indicates that the both synthesized nanoparticles have good purity with very less amount of impurities. EDX data indicates that Cu content was 54.56%, oxygen content was 33.75% in CuO nanoparticles and Zn determined by EDX was 40.77 and O was 45.82 in ZnO.Graphical

Effects of zinc dosage and particle size on gut morphology, tight junctions and TNF-α expression in broiler breeder hens.

This study was performed to evaluate the effects of different amounts and particle size of zinc oxide (ZnO) on villus height (VH), villus width (VW), crypt depth (CD) and VH to CD ratio (VH: CD), and expression of zonula occludens-1 (ZO-1), occludin (OC) and tumour necrosis factor-α (TNF-α) in broiler breeders. A total of 350 (Ross 308) broiler breeder hens of 54weeks randomly assigned to seven treatments, included control basal diet (C) without added Zn, C+ 100, and 130mg Zn per kg of diet from Large (L) (100-1000nm) and Small (S) (<100nm) particle size ZnO (LZnO100 and 130; SZnO100 and 130), C and SZnO100 challenged with lipopolysaccharide (C+LPS and SZnO100+LPS). Each diet was fed to five replicates consisting of ten birds each. The middle part of the duodenum, jejunum and ileum was used for morphological assessments. To assess the gene expression of ZO-1, OC and TNF-α in the jejunum samples were excised. Results showed that the supplementing 130ppm SZnO increased VH:CD in the duodenum (p<0.05). VW in the duodenum and all the evaluated morphometric indices in jejunum and ileum were not affected by the dietary treatment (p>0.05). ZO-1mRNA abundance in C+LPS group compared to SZnO100+LPS group was significantly decreased and increased by LPS and SZnO100 respectively. The SZnO-100 increased OC gene expression in compare to C+LPS group. The expression of TNF-α in C+LPS treatment was higher than other groups (p<0.05). The lowest and the highest litter moisture and foot-pad dermatitis (FPD) were observed in LZnO-130 and C treatments respectively (p<0.05). Improving the physical properties of ZnO affect on VH:CD. Broiler breeder diet with ZnO enhance ZO-1, OC and mitigate TNF-α gene expression in jejunum maintenance of gut health in broiler breeders.

The Effects of Nano and Micro Particle Size of Zinc Oxide on Performance, Fertility, Hatchability, and Egg Quality Characteristics in Laying Japanese Quail.

The current trial was conducted by using the 288 Japanese quails to evaluate the effects of a zinc-deficient diet supplemented with nano and micro zinc oxide on performance, fertility, hatchability, and egg quality characteristics. In this experiment, birds were randomly allocated to 9 dietary treatments includes diets supplemented with nano or micro particles of zinc oxide (amount of ZnO supplement for each treatment) to supply 49, 74, 99, and 124mg zinc per kilogram of diet in a factorial arrangement (2 × 4) and a control non supplemented diet (24mg/kg) with four replicates of eight birds (six females and two males) in each pen. Birds were fed the experimental diets from 47 to 75days of age and had free access to water and feed during the experimental period. Results showed that Zn supplementation, regardless of particle size, improved the eggshell thickness (P < 0.01). A significant (P < 0.05) interaction was observed between zinc level and ZnO particle size for Shell breaking strength. Quails fed diets supplement with ZnO showed significantly higher egg weight and eggshell surface (P < 0.05) as compared with birds fed a non-supplemented control diet. Results obtained here showed that supplementation of nano ZnO enhanced fertility considerably. Application of non-linear quadratic models showed that the maximum egg production percentage was achieved when 67 or 72mg/kg of dietary zinc was supplied from nano and micro ZnO, respectively. This result indicated that nano ZnO could reduce the zinc requirement in laying Japanese quail.

In Situ Observation at the Surface of Zinc in Alkaline Solution under Pulsed Current by Holographic Interferometry

Horizontal upward-facing zinc anode surfaces combined with Ni(OH)2/NiOOH cathodes in 1.95 M KOH aqueous solution were electrochemically dissolved and deposited with pulsed current. The morphological variations of zinc oxide were examined as a function of pulse width at three different locations on the anode surface using ex situ SEM observation. The particle size of zinc oxide varied with pulsed current for different pulse periods. Then, the transient variations in electrolyte for refractive index profiles along the axis perpendicular to the zinc anode were in situ measured under pulsed current using holographic interferometry. The oscillating layers of the refractive index profile were observed and related to a duplex diffusion layer model proposed by Ibl. Much more fine set up of SOC can be anticipated by combining the present results with HEV control (ECU) system.

The impact of film thickness on the properties of ZnO/PVA nanocomposite film

Polymer inorganic nanocomposites are attracting a considerable amount of interest due to their enhanced electrical and optical properties. The inclusion of inorganic nanoparticles into the polymer matrix results in a significant change in the nanocomposite’s properties. With this in mind, we have developed a nanocomposite film based on zinc oxide (ZnO) and polyvinyl alcohol (PVA) using a solution casting method with varying concentrations of ZnO nano powder in the PVA matrix. The ZnO / PVA film surface morphology was observed by the scanning electron microscope (SEM). The micrographs indicate that ZnO nanoparticles in the PVA matrix are homogeneously distributed. XRD results indicated that the crystallinity of the film was influenced by the interaction of ZnO nanoparticles and the PVA main chain. Crystallinity is also affected by the doping of ZnO nanoparticles in the PVA matrix and it increases when the concentration of ZnO is low and then decreases when the excess concentration of ZnO is present in the PVA matrix. The FTIR transmission spectra confirmed that significant interaction took place between the ZnO nanoparticles and the PVA main chain over the wave number range of 400–4000 cm−1. The UV–vis spectra reveal that the increase in concentration of ZnO nanoparticles in the polymer matrix results in the movement of the absorption edge in the direction of higher wavelength or lower energy associated with the blue/green portion of the visible spectrum. A decrease in the optical energy bandgap is observed with the increase in nano ZnO concentration in the matrix. Thickness has a signifcant affect on the properties of the ZnO/PVA nanocomposite and the morphology, particle size, degree of crystallinity and bandgap of the ZnO/PVA nanocomposite samples were influenced by the thickness of the sample. The optimal thickess of 0.03 mm with a weight percentage of 16.6% (ZnO) and 83.3% (PVA matrix) was selected due to its higher bandgap of 4.22 eV, reduced agglomeration/aggregation and smaller ZnO particle size of 14.23 nm in the matrix. The optimal film can be used in photovoltaic research.

Characterization of ZnO-TiO2 Hybrid Nano Eased SAE 15W-40 Oil

The main aim of the present investigation was to characterize the lubricant dispersed with ZnO-TiO2 hybrid nanoparticles. The work includes a dispersion of nanoparticles with varying proportions of 0.02 to 0.08 with an incremental step of 0.02 wt. % of base oil. Further, the samples were used to study the thermo-physical properties. The characterization result includes crystallinity and particle size of Zinc-oxide and titania nanoparticles, whereas the nano-based lubricant is characterized for its chemical compatibility, thermal conductivity, viscosity, and thermal stability. The result favors enhancing the thermo-physical properties of nano-based lubricant. Index Terms: nanoparticles, lubricant, thermal conductivity.

Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity.

Chitosan is promising material for making food packaging film with antimicrobial activity. However, chitosan film usually has limited mechanical and antimicrobial properties and higher water solubility. To improve the performance of chitosan film, in this work, chitosan composite films were prepared by incorporating different sizes of zinc oxide particles of 5μm, 100nm, and 50nm. The films were characterized by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and mechanical analysis. Antimicrobial assay of the chitosan and CTS/nano-ZnO composite films against Escherichia coli and Staphylococcus aureus show that the composite chitosan films have better antibacterial activity. The film containing 0.3% of 50nm zinc oxide particles showed the best inhibition rate, suggesting that smaller sizes of nano-ZnO particles have better bacteriostatic activity and potent application as an antibacterial additive ingredient.