Zaporizhzhia State Medical University Research Articles

Dynamics of prostaglandin E2 in the surgical treatment of gastroesophageal reflux disease

Gastroesophageal reflux disease (GERD) is one of the most common gastroenterological diseases. Therefore, the issues of diagnosis and the most effective treatment of GERD are extremely relevant. Achieving a stable positive result of treatment is impossible without taking into account the pathogenetic mechanisms of the development of GERD. Particularly relevant are the little-studied issues of the influence of humoral factors on the development of GERD in the course of treatment. One of the interesting biologically active substances is prostaglandin E2, the possible involvement of which in the mechanisms of the development of GERD is insufficiently reported. The aim of the study is to evaluate the effect of antireflux surgery on the level of prostaglandin E2 in blood serum and to verify that its changes after surgical treatment are associated with the decrease of gastroesophageal reflux and esophageal inflammation. Materials and methods. 35 patients were examined with GERD who underwent laparoscopic total antireflux fundoplication. There were 26 women (74.3 %) and 9 men (25.7 %). Their age is 55.3 ± 11.3. The control group consisted of 20 practically healthy people (women – 14 (70.0 %), men – 6 (30.0 %), average age – 56.7 ± 10.6). Immunoenzymatic analysis of prostaglandin E2 was performed in blood plasma, which was obtained according to a stan­dard method. Determination of prostaglandin E2 (Prostaglandin E2 ELISA, KGE004B, RnD Systems) was carried out by the immunoenzymatic method based on the use of the “sandwich“ variant of the solid-phase immunoenzymatic analysis. The procedure was carried out on the immunoenzyme complex ImmunoChem-2100 (USA) at the Department of Clinical Laboratory Diagnostics in Zaporizhzhia State Medical University. Research on the level of prostaglandin E2 in the main group was carried out before surgical treatment and 2–3 months after surgery by taking venous blood and using the above test systems. Statistical evaluation of the research results was performed using the Statistica for Windows 13 software package (StatSoft Inc., No. JPZ804I382130ARCN10-J). Results. The level of prostaglandin E2 in the blood of practically healthy people was 16.7 ± 6.1 pg/ml. In the main group, the values of prostaglandin E2 before surgical treatment were 25.8 ± 5.7 pg/ml, after surgical treatment, they decreased to 13.5 ± 5.3 pg/ml. The detailed analysis of patients in the main group showed that the level of prostaglandin E2 did not differ statistically in different erosive forms of esophagitis, or CLE and NERD. But it is statistically different from the level of prostaglandin E2 in practically healthy individuals of the control group. The conducted correlation analysis indicated that the level of prostaglandin E2 did not depend on the duration of acid exposure in the esophagus, as well as on the severity of esophagitis or the presence of CLE. Conclusions. With effective surgical treatment of gastroesophageal reflux disease, a decrease in the level of prostaglandin E2 after surgery is determined compared to preoperative data to the level obtained in a group of practically healthy patients. The obtained dynamics of the level of prostaglandin E2 indicates the possibility of this hormone influencing the tone of the lower esophageal sphincter and active participation in the pathogenesis of GERD, which confirms the possibility of its use as an additional diagnostic marker of inflammation in the esophagus and a marker of the effectiveness of surgical treatment.

Effectiveness and safety of a modified short-term regimen of antimycobacterial therapy to treat rifampicin-resistant tuberculosis in elderly patients with concomitant diabetes (a clinical case)

Aim. To analyze the effectiveness and safety of a modified short-term regimen (mSTR) of antimycobacterial therapy (AMBT) for rifampicin-resistant tuberculosis (RR-TB) in a 71-year-old patient with type 2 diabetes mellitus (T2DM) on the clinical case example of own observation. Materials and methods. A clinical case from own observation of the patient who was treated at the clinical base of the Department of Phthisiology and Pulmonology of Zaporizhzhia State Medical University – Pulmonary Tuberculosis Department No. 2 of the Communal Non-profit Organization “Zaporizhzhia Regional Phthisio-Pulmonology Clinical Treatment and Diagnostic Center” is presented. Results. The presented case demonstrates the high safety and efficacy of all oral mSTR, including Lfx-Bdq-Lzd-Cfz-Cs, in the elderly person with RR-TB and concomitant decompensated T2DM who was smear-negative after 4 months as a result of treatment with 9-month mSTR AMBT (Lfx, Bdq, Lzd, Cfz, Сs). Positive radiological dynamics were observed all the time and residual changes in the lungs after tuberculosis were diagnosed at the end of the treatment course. These results complement indications for the use of mSTR, including Lfx-Bdq-Lzd-Cfz-Cs, in RR-TB patients. Conclusions. mSTR AMBT (Lfx, Bdq, Lzd, Cfz, Сs) is effective and safe in elderly patients with RR-TB and concomitant decompensated type 2 diabetes mellitus when adequate treatment of diabetes and timely correction of antimycobacterial drug side effects are undertaken.

Comparative studies of the specific activity of soft dosage forms minoxidil for trichological practice

The treatment and prevention of hair loss have been a serious aesthetic problem for the population for a long time due to the widespread occurrence of the condition and its undesirable medical and social consequences. The pharmacotherapeutic options for trichological practice are very limited, and the effectiveness of many of these drugs is small or questionable. The peripheral vasodilator minoxidil is one of the few medicinal substances that has been proven to stimulate hair growth. However, the number of minoxidil preparations allowed by the Ministry of Health for use in Ukraine is minimal and mainly represented by rare dosage forms for external use. Considering the above, the employees of the Department of Medicines Technology at Zaporizhzhia State Medical University have proposed rational formulations and technologies for two new soft dosage forms of minoxidil – a 2 % cream for external use based on an emulsion, and a shampoo in gel form – based on complex physical-chemical, pharmaco-technological, rheological, and biopharmaceutical research. These formulations are intended to stabilize hair growth and restore hair coverage in men and women suffering from androgenic alopecia. The aim of this research is to study the specific activity of the proposed topical dosage forms of minoxidil. Materials and methods. Experimental cream with 2 % minoxidil on an emulsion carrier and a gel-like shampoo containing 1 % minoxidil combined with phytoextracts of hops and burdock, as well as corresponding placebo compositions, were used as objects of preclinical studies. The compositions of the topical minoxidil pharmacotherapeutic agents are scientifically based, ensuring their optimal pharmacotechnological, biopharmaceutical, and consistent consumer properties. The study of the specific activity of soft medicinal forms of minoxidil for external use was carried out by evaluating their growth-stimulating and follicle-protective activity. “Pilfood Bosnalek” spray for external use (Bosnalijek, Bosnia and Herzegovina), containing 2 % minoxidil, was used as a reference drug when studying the growth-stimulating effect of 2 % cream for external use with minoxidil. The reference drug in the study of the similar activity of the gel-like shampoo “Trichlomine” was the shampoo against hair loss “Dove” (Unilever, Germany). Results. It has been found that a 2 % minoxidil cream for topical use, when used together with the reference drug “Pilfood Bosnalek” (Bosnalijek, Bosnia and Herzegovina), has a significant hair growth-stimulating effect, which statistically significantly increases hair growth in experimental animals from as early as 3–4 days of use. The shampoo “Trichlomine” also has a significant effect on hair growth in experimental animals, with results observed from 3–4 days of use. Furthermore, the strength of the hair growth-stimulating effect observed from the use of “Trichlomine” shampoo significantly exceeds that of the reference “Dove” shampoo (Unilever, Germany). These results demonstrate the presence of significant follicle-stimulating activity in the tested minoxidil preparations for treating experimental alopecia and their positive effects on the weight and structure of laboratory animal fur. The follicle-protective activity of experimental minoxidil dosage forms (2 % cream and gel-like shampoo) was also experimentally confirmed, due to the regulation of various metabolic pathways in the dermis. The use of 2 % minoxidil cream and comparison preparations (“Dove” shampoo and “Pilfood Bosnalek” spray) did not result in the normalization of the investigated indicators of dermal energy metabolism. However, a positive effect of “Trichlomine” shampoo was found, which is associated with the presence of a plant antioxidant complex in the pharmacotherapeutic agent and its potentiating effect with minoxidil. Conclusions. It was found that the application of soft dosage forms of minoxidil (2 % emulsion-based cream and 1 % gel-based shampoo) exhibited pronounced growth-stimulating and follicle-protective activity in the model of experimental alopecia in white rats. At the same time, it was established that the cream with minoxidil 2 % for external use and the reference spray for external use “Pilfood Bosnalek” (Bosnalijek, Bosnia and Herzegovina) have a significant statistically comparable growth-stimulating effect. The strength of the growth-stimulating effect recorded from the use of the “Trichlomine” shampoo significantly exceeded the effect of the reference shampoo “Dove” (Unilever, Germany). It was found that both experimental medicinal forms of minoxidil (2 % cream and gel-like shampoo) had a follicle-protective effect by normalizing various links of dermis metabolism. The most pronounced effect in this regard was recorded in the shampoo “Trichlomine”, which contained not only minoxidil but also plant-antioxidant complexes. Application of 2 % cream with minoxidil, as well as reference drugs (spray “Pilfood Bosnalek” and shampoo “Dove”), did not have a reliable effect on the indicators of energy exchange of the dermis studied.

Bedaquiline resistance in Mycobacterium tuberculosis during antimycobacterial therapy and its impact on clinical outcomes (clinical cases)

Aim. To analyze the nature of Bedaquiline (Bdq) resistance in Mycobacterium tuberculosis (MBT) during antimycobacterial therapy (AMBT) in patients with multidrug-resistant tuberculosis (MDR-TB), its impact on clinical outcomes and to detect risk factors for the Bdq drug resistance development on the example of own observed clinical cases. Materials and methods. 2 clinical cases of own observations of the Bdq resistance development in MBT during AMBT in patients with MDR-TB who were treated at the clinical base of the Department of Phthisiology and Pulmonology of Zaporizhzhia State Medical University – Pulmonary Tuberculosis Department No. 2 of the Communal Non-profit Organization “Zaporizhzhia Regional Phthisio-Pulmonology Clinical Treatment and Diagnostic Center” are presented. Results. The presented clinical cases confirm the literature data that the treatment effectiveness in patients with Rifampicin-resistant TB (RR-TB) and MDR-TB is associated with Bdq drug resistance. Regarding the reasons for the development of acquired Bdq drug resistance, the second clinical case, like the examples of other authors, demonstrates low adherence to treatment. There is no data in the literature about the lack of controlled treatment at the outpatient stage as a risk factor for Bdq drug resistance and negative clinical outcomes in patients with MDR-TB, which would complement the scientific experience in this problem. Conclusions. In both clinical cases, Bdq drug resistance was acquired (secondary), and had an impact on the clinical outcomes of tuberculosis treatment. The risk factors for Bdq drug resistance and negative clinical outcomes were lack of controlled treatment at the outpatient stage in the first case and low adherence to treatment in the second one.

SERUM CYSTATIN C AS A PREDICTOR OF THE DEVELOPMENT OF ACUTE KIDNEY INJURY IN NEWBORNS WITH HYPOXIC- ISCHEMIC ENCEPHALOPATHY SUBMITTED TO THERAPEUTIC COOLING

Assessment of renal function in newborns is extremely important and at the same time challenging due to the unique body structure, increased vulnerability and rapid growth of the latter. However, for the early detection of acute kidney injury (АКI), rational dosing of drugs and safe drug therapy, the identification of early markers of renal dysfunction is essential.The objective is to evaluate the prognostic value of serum biomarkers for the early diagnosis of АКI in newborns with hypoxic-ischemic encephalopathy against the background of therapeutic hypothermia and preventive use of methylxanthines.Materials and Methods. A single-center, prospective, randomized trial involving 44 neonates with АКI requiring therapeutic hypothermia and prophylactically receiving caffeine citrate or theophylline to prevent АКI progression was conducted in from 2019 to 2022 on the basis of the NICU of Zaporizhzhia Regional Clinical Children's Hospital.Laboratory analysis of blood serum samples was performed on day 1, day 3 and 5 from birth, creatinine (Cr) and cystatin C (CysC) levels and their associations with the development of АКI were determined according to the neonatal criteria of the 2012 KDIGO guideline.Statistical analysis was performed using Statistica 13.0 program, TIBCO Software Inc. (license number JPZ804I382130ARCN10-J) and Microsoft Excel 2013 (license number 00331-10000-00001-АА404). The probability of the difference in absolute values of mean values was determined using non-parametric methods of statistical analysis: the Mann-Whitney U-Test for unrelated groups and the Wilcoxon signed-rank t test for related groups. Statistical significance was defined as p < 0.0500.The study was performed in accordance with the moral and ethical standards established by the IGH / GCP guidelines, the World Medical Association Helsinki Declaration, adopted in 1964 and amended in 1975, 1983, 1989, 1996 and 2000, The European Convention of Human Rights and Biomedicine and the legislation of Ukraine. The protocol was approved by the Medical Ethics Commission at Zaporizhzhia State Medical University. The study was performed as part of the research project "Optimization of diagnostics and intensive care of polyetiologic lesions of the brain, gastrointestinal tract, and kidneys in newborns and older children" (State registration number O118U007142) of the Pediatric Surgery and Anesthesiology Department of the State Institution "Zaporizhzhia State Medical University of the Ministry of Health of Ukraine."Results and their discussion. In general, AKI according to KDIGO developed in 5.00 (11.36 %) neonates out of 44.00 (100.00 %), stage 0 was found in 39.00 (88.64 %). 4.00 (9.09%) newborns had stage I, and 1 (2.27%) developed stage II; the data obtained were similar: p = 0.7872; U = 230.00. None of the patients progressed to stage III.In the newborns with preserved renal function during the study there was a decrease in Cr and a predictable, by this marker, increase in GFR. A statistically significant increase in Cr level and decrease in GFR was found in the newborns with renal dysfunction on days 3 and 5 of the study. Cr level progressed from baseline 1.07 (0.87; 1.10) mg/dl to 1.13 (0.86; 1.25) mg/dl on day 3 and to 1.40 (1.15; 1.82) mg/dl on day 5, while GFR decreased from 19.76 (19.07; 22.90) ml/min/1.73m2 to 17.97 (13.84; 24.42) ml/min/1.73m2 on day 3 and was 12.38 (11.12; 17.54) ml/min/1.73m2 on day 5, with p < 0.0500.CysC progressively decreased in the neonates without AKI from 2.50 (2.20; 2.60) ng/ml to 2.25 (2.08; 2.49) ng/ml, p = 0.0095; while in the neonates with AKI the level of this marker did not change and was 2.56 (2.41; 2.70) ng/ml on day 1 and 2.42 (1.89; 2.45) ng/ml on day 5, p = 0.2963. As this marker changed, eGFR (CysC) increased progressively in the cohort of patients without kidney damage but did not change in the other group.The diuresis rates in the newborns of both groups did not differ, being ≥ 1.5 ml/kg/h, which is probably due to methylxanthine therapies, p ≥ 0.0500.Conclusions. CysC assessment did not provide additional information on the development of acute kidney injury in neonates (nAKI) in the first 5 days of life, which would have allowed a quick decision to change the intensive care program. Further studies involving newborns who did not receive prophylactic therapy are needed.

INDIVIDUAL FACTORS OF MEDICAL PHD STUDENTS' ENGLISH COMMUNICATIVE COMPETENCE.

The aim: The paper is aimed to find correlations between English language communicative academic and medical competence components (theoretical, practical, and individual by certain methods), to improve design of the course "Academic English for PhDs in Medicine", its methods and strategy. Materials and methods: The study sample includes postgraduate groups studying for PhD in Healthcare, aged 21-59 years, in Bukovinian State Medical University (39 respondents), Zaporizhzhia State Medical University (32 respondents), Kharkiv Medical Academy of Postgraduate Education (33 respondents), and Bogomolets National Medical University (318 respondents). The study was held in 2019-2023. We assessed theoretical and practical components using our tests, individual component - psychological methods. The values of three components were transformed into general English communicative academic and medical competence level. The data were treated with SPSS Statistica 18.0, using Spearman correlation significance. Results: We found positive correlation between English communicative competence and communicative tolerance, general communicative skills level, and high or medium communicative control level. Also, positive correlation characterizes interaction as a conflict-resolving strategy and communicative competence. Highly manifested intolerance in communication, predominance of negative mindsets, intolerance to stress negatively affect the level of English communicative academic and professional competence of PhD students. Conclusions: Analysis of the English speaking competence, and its components, showed positive correlation between interaction as a conflict-resolving strategy and English communicative competence of respondents. Regarding the results, the curriculum of the "Academic English for Medical PhDs" should be modified, including interactive methods, case studies, problem solving, and other methods for individual component training.

A study of hypoglycemic activity of acids and salts containing 1,2,4-triazole

This article presents the study results of the hypoglycemic properties among 1,2,4-triazole derivatives synthesized at the Department of Physical and Colloidal Chemistry of the Zaporizhzhia State Medical University. Today, many people have a sedentary way

Практичне застосування високоефективної рідинної хроматографії та високоефективної тонкошарової хроматографії у фармацевтичному аналізі.

The purpose of this work is to analyze the chronology and state of development of chromatographic methods at the Zaporizhzhia State Medical University (ZSMU). A historical overview of the use of chromatographic methods at ZSMU is provided. First of all, emphasis is placed on the introduction of high-performance liquid chromatography and gas-liquid chromatography. The main scientific laboratories and groups that worked and are working at the Zaporizhzhia State Medical University in the field of instrumental chromatography are shown. There were groups at the Department of Biological Chemistry, the Department of Faculty Pediatrics, the Department of Physical and Colloidal Chemistry. Currently, the Department of Physical Colloid Chemistry is actively working on a new laboratory of liquid chromato-mass spectrometry of the Department of Experimental Pharmaceutical Research of the training medical-laboratory center. The laboratory performs research for many departments of ZSMU, including the Faculty of Pharmacy, the Faculty of Postgraduate Education, the Faculty of Medicine. The laboratory develops, validates and uses methods to establish the purity and confirm the molecular weight and structure of new biologically active substances synthesized to search for new drugs, research of impurities in drug substances, determination of biologically active substances in extracts of medicinal plant raw materials, determination of residual amounts drugs in poultry meat and eggs, and other livestock and poultry products, determination of concentrations of disease markers, determination of distribution, metabolism and excretion of drugs.

Comparative study of the specific activity of soft application drugs with vinpocetine

In modern neurology, the main component of successful treatment of cerebrovascular pathology is pharmacotherapy. At the same time, the arsenal of effective medicines of evidence-based medicine in this area is quite limited. Vinpocetine (Cavinton) is one of the drugs that effectively selectively corrects cerebral perfusion. The use of alternative ways of absorption of vinpocetine (rectal and nasal) in certain soft application dosage forms will allow to significantly increase the bioavailability of the medicinal substance, reduce the level of adverse reactions from its use and expand the pharmacotherapeutic arsenal of domestic effective neuroprotective drugs. Taking into account the above, the employees of the Department of Medicines Technology of Zaporizhzhia State Medical University, on the basis of complex physical-chemical, pharmacotechnological, rheological and biopharmaceutical research, proposed rational formulations and technologies of two new soft application dosage forms of vinpocetine – rectal suppositories on a lipophilic basis and nasal cream on an emulsion basis for the therapy of arterial hypertension of II–III stages with cerebrovascular complications, reduction of the manifestation of neurological and/or mental symptoms in various forms of cerebral blood circulation insufficiency, consequences of a stroke, transient ischemic attack, etc. The aim of the work is to study the specific activity of the proposed innovative application dosage forms of vinpocetine. Materials and methods. Nasal ointment containing 0.5 % vinpocetine on an emulsion basis and rectal suppositories with vinpocetine 0.01 g on a lipophilic carrier were used as the object of preclinical studies, which ensure optimal release of the active pharmaceutical ingredient. The study of the specific activity of the developed medicinal forms of vinpocetine was carried out on a model of acute cerebrovascular accident of the type of ischemic stroke. Vinpocetin tablets (Astrapharm, Ukraine), which are used in neurological practice quite effectively and over a long period of time, were used as a comparison drug. Results. The comparative results showed that the appointment of all studied drugs led to an increase in motor activity. In addition, the administration of medicinal forms of vinpocetine reduced the development of cognitive and cognitive disorders after acute disorders of cerebral blood circulation. It was found that the highest result was shown by the group of animals that received a course of vinpocetine intranasally. In the groups of animals that received vinpocetine for 4 days, markers of oxidative stress – aldehyde phenylhydrazones and ketone phenylhydrazones – were significantly reduced. The highest activity was demonstrated by the pharmaceutical form of vinpocetin, which was used intranasally for 4 days. The introduction of dosage forms of vinpocetine exerted an energizing effect, which was expressed in an increase in the level of macroergic phosphates. Evidently, vinpocetine has a direct positive effect on the three-carbon sections of the Krebs cycle and increases the production of adenosine triphosphate (ATP). At the same time, the vinpocetine nasal cream applied intranasally for 4 days demonstrated the greatest energizing activity. Conclusions. Preclinical studies of the developed application soft medicines with vinpocetine on the model of acute cerebrovascular accident type ischemic stroke experimentally confirmed the expressed neuroprotective activity of rectal suppositories and nasal cream with vinpocetine and it was found that the effectiveness of therapy with these application medicinal forms is demonstrably higher than that in comparison with oral tablet means.

Features of the simultaneous course of pulmonary aspergillosis and tuberculosis on the background of type 1 diabetes mellitus (clinical case)

Aim. To analyze the features of the simultaneous course of pulmonary aspergillosis and multidrug-resistant pulmonary tuberculosis (MDR-TB) on the background of type 1 diabetes mellitus, on the example of a clinical case of our practice. Materials and methods. Clinical case of our practice of simultaneous pulmonary aspergillosis and MDR-TB on the background of type 1 diabetes mellitus in a patient, who was treated in Pulmonary tuberculosis department No. 2 of clinical base of Phthisiatry and Pulmonology Department of Zaporizhzhia State Medical University at Communal Non-Commercial Enterprise of the “Zaporizhzhia Regional Clinical and Diagnostic Center of Phthisiatry and Pulmonology” of Zaporizhzhia Regional Council. Results. In the presented clinical case, the patient had been suffering from diabetes mellitus for 8 years and had been receiving insulin therapy for all these years. One year before the TB disease, he had contact with an index patient, but he categorically refused a course of preventive chemotherapy. Also, the patient did not make a comparison X-ray TCO after 6 months, as a contact person. MDR-TB and aspergilloma of the left lung of the patient were diagnosed simultaneously on the background of severe condition with decompensated form of type 1 diabetes mellitus, severe intoxication syndrome and inflammatory process, massive hemoptysis. Aspergilloma of the left lung was diagnosed using X-ray TCO and cultural examination of bronchial aspirate for Aspergillus, which was not difficult to diagnose. Diagnosis of MDR-TB was also not difficult, because the patient had MBT, which contributed to the timely and correct appointment of antimycobacterial therapy. Surgery for aspergilloma of the left lung was contraindicated, as the type 1 diabetes was in decompensation. Persistent hyperglycemia remained, despite the fact of antifungal therapy and constant correction of insulin therapy prescribed for the patient. Endocarditis quickly developed in the patient, which was the reason of patient’s death. Conclusions. The decompensated form of type 1 diabetes mellitus caused persistent hyperglycemia, which was the reason of immune disorders and this made the patient’s body susceptible to bacterial (MDR-TB) and fungal (aspergillosis) infections, which led to the development of the complication of endocarditis and death. A big mistake in his case, was a categorical refusal by the patient to receive a course of preventive chemotherapy, as a contact person with an index patient. In view of this, in the presence of type 1 diabetes, the patient should have not neglected it. And as a result, the patient had a MDR-TB, one year after. At the same time, the patient did not make a comparison X-ray TCO, after 6 months, as a contact person, which was a possible reason for the missing of early diagnosis of pulmonary aspergilloma. That’s why, a correct treatment of type 1 diabetes mellitus and timely preventive radiological examination of the thoracic cavity organs are especially important, as the diabetes mellitus is the most common premorbid background for TB and aspergillosis.

Comparative study of specific activity of the piroctone olamine semisolid dosage form for external use

The external treatment of seborrheic dermatitis of the scalp depends on the withdrawal of clinical symptoms and the complaints the patient made to the doctor. At present Ukrainian dermatology has several dozen pharmacotherapeutic products in this direction, with dominating medicines of ketoconazole, zinc pyrition, and their combinations. They are characterized by the short-term or weak effect, associated with the pathogenic microorganisms’ resistance against the background of long-term use of biologically active substances which are their active parts. One way to solve the problem is using the drugs with new highly active pharmaceutical ingredients. Employees of the Department of Technology of Medications of the Zaporizhzhia State Medical University proposed the composition of a new applicate semisolid dosage form of pirocton olamine based on complex physical-chemical, pharmacotechnological, biopharmaceutical, and rheological investigations – topical ointment on the hydrophilic base in combination with resin-free naphthalene for external use in the complex etiotropic treatment of seborrheic dermatitis of the scalp. The aim of this work is to study the specific activity of the combined ointment with pirocton olamine and resin-free naphthalene on a hydrophilic vehicle. Materials and methods. As the object of preclinical studies used experimental ointment containing 1 % piroctone olamine and 5 % resin-free naphthalene on the base of sodium carboxymethylcellulose glycerogel, providing optimum release of active pharmaceutical ingredients from the vehicle, and appropriate placebo semisolid dosage form. The study of the specific activity of the developed ointment under the conditional name “Oktonaf” was carried out by evaluating its antimicrobial, anti-inflammatory, and antioxidant effects given the foreseeable therapeutic focus and the literature on the spectrum of biological activity of active substances. The anti-inflammatory and antioxidant action of the proposed composition was evaluated by the allergic contact dermatitis model. As a reference drug cream “Psoricap” (KMP, Ukraine), which has long been applied in dermatological practice was used. Results of comparative microbiological studies proved that composite anti-seborrheic ointment with piroctone olamine and resin-free naphthalene by the antibacterial effect in relation to the size of the growth delay zones for Staphylococcus aureus and Escherichia coli (gram-positive and gram-negative microorganisms) slightly exceeds the effective dermatological medication reference cream “Psoricap”. At the same time, the antimycotic activity of experimental ointment in relation to Candida albicans practically does not differ from the same reference drug. A study of antioxidant and anti-inflammatory activity of the developed piroctone olamine ointment on a hydrophilic basis using the model of allergic contact dermatitis revealed that its level reliably was exceed the efficacy of topical reference medication “Psoricap”. Conclusions. Obtained data of a spectrum of specific activity of a combined ointment with piroctone olamine and resin-free naphthalene can predict the high clinical value of the proposed drug for practical domestic dermatology.

Productivity and Quality of Broiler Chicken Meat Using new Triazolin Compounds

In providing the population with high-quality food products, a special place is given to poultry meat production. The purpose of this study was to investigate the effect of triazoline compounds on the productivity and haematological parameters of broiler chickens. The study was conducted in 2021–2022 at the Odesa State Agrarian University in vivarium and analytical laboratories. 1,2,4-triazole derivatives synthesised at the Zaporizhzhia State Medical University were used for the study: GKPF-109 – Morpholin-4-ium-2-((4-amino-5-(3-methyl-pyrazol-5-yl) -1,2,4-triazol-3-yl)thio)acetate; ASP-34 – sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl)thio)acetate. The dynamics of poultry growth was determined by individual weighing, followed by determination of absolute and average daily live weight gains. Poultry feeding was carried out according to species and age periods per the existing norms. Haematological, immunological, and biochemical studies were performed according to the following methods.Tasting evaluation of meat and broth was carried out according to a 5-point system. Broths were evaluated according to 4 indicators – taste, smell, colour, transparency, each of them according to a 5-point system. The analysis of total weight data in the groups indicates the highest gross gain in live weight in the group of 36-day-old chickens that received GKPF-109 from 10 days of age, which is 14,966 g in 26 days of the experiment. The preservation of broiler chickens during the experimental period was 96% in the experimental groups, and 94% in the control group. Analysing the above, it can be concluded that adding research compounds of the triazoline series to drinking water increases haematopoiesis, has anti-inflammatory and hepatoprotective effects. Evaluation of the quality of meat of broiler chickens and broth at the end of the experiment does not allow claiming a decrease in their aroma and taste qualities, which indicates the absence of a negative effect of the research compounds of the triazoline series and the methods of their application on the organoleptic parameters of the meat.

Aeromonitoring of Alternaria spores in the air of Zaporizhzhia city

The aim of the study was to determine the patterns of changes in the concentration of mold spores of the genus Alternaria in the air of Zaporizhzhia city. Materials and methods. The object of the study was the dynamics of Alternaria spore concentration in the atmospheric air of Zaporizhzhia city. The study was carried out at the Department of Medical Biology, Parasitology and Genetics of Zaporizhzhia State Medical University. Samples were taken annually from March 1 to October 31 by volumetric method using a Hirst-type trap. The device is yearly certified by the state metrological service with certificates. The samples were analyzed using a light microscope at ×400 magnification using the method of vertical transects. The results of the research were processed by applying the Statistica® for Windows 13.0 licensed software package. Results. The seasonal dynamics of fungal spores of the genus Alternaria in the atmospheric air of Zaporizhzhia was analyzed over several years: from 2015 to 2020. The beginning, end and duration of sporulation, maximum values were determined. Conclusions. Analyzing the average daily (for six years) indicators of the spore number in the air of Zaporizhzhia city, it can be concluded that their distribution is normal throughout the year, which corresponds to the intensity of spore formation, depending on changes in meteorological conditions, that is, an almost equally symmetric range from the optimum, which falls on the beginning of June and is associated with an increase in precipitation in this region. Thus, an average of 17 256 spores/m3 were determined over six years. The maximum number of spores was 680 spores/m3 (6.07). The average day fell on July 25, σ = 37.1. In general, sporulation lasted 119 days. Peak concentrations were recorded in summer and autumn.

Pulse oximeters market analysis during the COVID-19 pandemic: Kyiv pharmacies’ offers and survey of pharmacy faculty students

Abstract Objectives The objective of this study was to study the market of pulse oximeters to select the optimal choice for monitoring health status during the COVID-19 pandemic. Methods Pulse oximeter data, presented on the Tabletki.ua website (2021) in Kyiv (Ukraine), were summarized and discussed. A survey about the history of the device purchase among 170 students of the Faculty of Pharmacy (Zaporizhzhia State Medical University, Ukraine) was statistically analysed by Kolmogorov–Smirnov, Shapiro–Wilk, Levene’s, Scheffe tests, one-sample t-test, Spearman’s correlation and one-way ANOVA. Key findings The largest part of the Kyiv (Ukraine) pulse oximeter market is occupied by the People’s Republic of China (70.97%). The price ranged from UAH 171.50 to UAH 1799.00. The models S6 and Linke LK88 are found to be the best choices according to the fullest provided data, the lowest minimum price and emphasis on the oxygen saturation and pulse rate accuracies. Medical students statistically significant would buy a pulse oximeter for less than UAH 563.40 (t(76) = −2.884; P = 0.005). The price did not depend on age (F = 1.104, P = 0.372) or gender (F = 2.466, P = 0.121), but there was a correlation between price and year of purchase (F = 2.712, P = 0.051). Students are primarily interested in country of origin, guarantees and pharmacist recommendations. A significantly weak Spearman’s correlation was found between price and friend advice (ρ = 0.275, Sig. = 0.015). Conclusions The same models differ in price by up to 4.2 times and in between them – up to 10. Less than half of the responders bought devices, and even a quarter of them did not use them at all, although they were from a health field. The detailed notifications about accuracy for different patient skin types are required in the device description. More people should be aware of pulse oximeter importance to maintaining health after COVID-19.

Spectrophotometric methods for quantitative determination of sotalol in tablets

The aim of the work. To develop a method for spectrophotometric determination of sotalol with diazonium salts. Establish optimal conditions for the quantitative determination of sotalol in drugs. Validate the developed methodology. Materials and methods. Reagents and solvents used in the study were: diazole red 2J (obtained from NVF “Sinbias”), tablets “Sotalol Sandoz” 40 mg (Salyutas Pharma GmbH, series JZ1188), “Sotalol Sandoz” 80 mg (Salyutas Pharma GmbH, series KA0464) and “Sotalol Sandoz” 160 mg (Salyutas Pharma GmbH, series JY3504), methanol (LAB-SCAN, Ireland, batch № 5120/13), sodium carbonate (Sinbias) and purified water were also used. Analytical equipment: spectrophotometer “SPECORD-200” (Analytic Jena AG, Germany), scales laboratory electronic RADWAG XA 210.4Y, bath ultrasonic Sonorex Digitec DT100H, laboratory glassware of class A. All studies were conducted in the experimental pharmaceutical research department of the scientific medical laboratory center (SMLC) of the Zaporizhzhia State Medical University. Results and discussion. The technique of spectrophotometric determination of the quantitative content of sotalol based on its reaction with red diazole in water-methanol medium has been developed. The stoichiometric ratios of the reactive components as 1:1 were obtained by the methods of continuous changes and the saturation method. Validation of the developed on such indicators as linearity, precision, correctness and robustness is carried out. Based on these data, the developed method is correct and could be used in the quality control departments of chemical and pharmaceutical companies. Conclusions. A method of quantitative spectrophotometric determination of sotalol in the tablet dosage form “Sotalol Sandoz” 40 mg, “Sotalol Sandoz” 80 mg and “Sotalol Sandoz” 160 mg of industrial production was developed, validation characteristics were investigated: linearity, precision, correctness, range of application and robustness

Вплив тригерних факторів на клінічну розповсюдженість вогнищ себорейного кератозу

The purpose of the study was to determine the effect of ultraviolet radiation on the risk of seborrheic keratosіs, assessing the level of correlation between anamnestic and clinical data (skin phototype, frequency of sunscreens) and the extent of neoplasms on the skin. Materials and methods. Sixty patients with seborrheic keratosіs were examined on the basis of "University clinic" of Zaporizhzhia State Medical University. Preliminary verification of the diagnosis was performed using visual examination, dermatoscopic examination based on a three-point algorithm in polarized light mode (FotoFinder Bodystudio ATBM (Germany). Clinical examination also included Fitzpatrick assessment of skin phototype, taking into account skin color, eye, hair color, and tendency to form tanning or its complications (burns). Results and discussion. Majority of patients were of II skin phototype – 40 (66.7%) individuals, III – 19 (31.7%), IV – 1 (1.7%). 37 patients (61.6%) with seborrheic keratosіs received skin burns after prolonged exposure to the open sun. The most frequent localizations of keratoma were areas of the head – 23 (38.33%) patients, torso – 17 (28.33%), limbs – 16 (26.67%), neck – 4 (6.67%). Thus, taking into account the areas of maximum ultraviolet exposure – head + neck + limbs – we have the majority of cases (n = 43 (71.7%)) with a possible risk factor in the form of increased insolation on these areas of skin. Only 1 patient lived in a country with increased insolation for 0.5 years. 29 (48.4%) patients never used sunscreens, 19 (31.6%) used them occasionally, and 12 (20%) always used them. Pearson's coefficient (Kp =0.51) and Chuprov's coefficient (Kh =0.50) indicate a moderate relationship between the number of foci and Fitzpatrick skin phototype. The data obtained do not rule out an association between the level of lesion foci dissemination and skin features to the perception of tanning. The association coefficient was 0.714 and the contingency coefficient was 0.4. Consequently, this suggests that the association between the use/non-use of sunscreen and the multiplicity of keratosis foci is significant. These data have not been highlighted in previous studies, so we believe that this is an additional argument for the mandatory use of sunscreen. Regular use of sunscreeens has really a significant protective potential for the occurrence of new skin formations, as well as their clinical dissemination. Conclusion. Ultraviolet radiation may be a predictor of multiple foci of keratosis. It is important to comprehensively examine patients with seborrheic keratosіs, focusing on evaluation of skin phototype, history of outdoor exposure, and sunbathing habits. The use of sunscreen remains the unequivocal leader in the prevention of multiple seborrheic keratosis

Classification of stressful factors associated with COVID-19 pandemic and quarantine among Ukrainian military personnel

The COVID-19 pandemic and quarantine have caused psychological distress, which has led to mental and physical health problems and changes in the behavior patterns of many people. It is known that the cause of such stress in the civilian population is not a pandemic or quarantine in general, but only some of their components. However, although COVID-19 pandemic and quarantine can provoke health disorders also in military personnel, stressful factors directly triggering these disorders have not yet been identified. Aim: to identify the immediate stressors that threaten Ukrainian military personnel health during the COVID-19 pandemic and quarantine. Materials and methods. The study was conducted at the Zaporizhzhia Military Hospital and Zaporizhzhia State Medical University between March 2020 and May 2020. A total of 25 military personnel hospitalized during this period were examined. Clinical, psychopathological, psychological examinations was conducted. The Fear of COVID-19 Scale was also used. Results. The stressful factors associated with the COVID-19 pandemic and quarantine, which had provoked health disorders in military personnel, have been identified and systematized into four groups: vital fears, social fears, domestic and organizational stress, abstract experiences. All these groups have been also described here. Conclusions. Identification of psychogenic factors will help to determine targets for psychotherapy and reduce the negative effects of the COVID-19 pandemic and quarantine and prevent a decline in fighting capacity.

COMPONENT MODEL OF THE CLOUD-ORIENTED LEARNING ENVIRONMENT OF A MEDICAL UNIVERSITY

The rapid development of innovative methods of learning, which are based on the widespread usage of information and communication technologies, forms the conditions for the transformation of the system of higher medical education. The emergence of cloud services in the IT services market has opened up new opportunities for the development of higher education institutions' (HEI) infrastructure. The quarantine crisis has made medical university faculty to begin restructuring the infrastructure of higher education institution in the direction of implementing the latest technologies of learning. During the COVID-19 quarantine period, medical universities started using Microsoft and Google cloud services extensively to provide distance learning to medical and pharmacy students. At the stage of adapting these services to the organization of the learning process, the problem was that there were no developed models of cloud-oriented learning environments for medical HEIs, which take into account the specifics of teaching subjects at medical faculties. In developing the component model, we applied methods of system analysis and approaches to describing the departments of the university from the perspective of an invariant model of the organizational system. This model allows scaling the technology of forming a hybrid cloud-oriented environment to all departments of the university. The component model of cloud-oriented educational environment was based on the model of the pedagogical system, which consists of the target, teaching, methodological, student, content and technological components. During the analysis of organization of medical education system of the university by method of stratification four main system levels were identified: І- strategic planning and organization of studies at the university; ІI- tactical planning and organization of medical education according to specialties; ІІI- planning and organization of educational process for students at 62 departments of the university; IV - modeling of partner interaction in the process of study on the basis of subject-subject relations of the teacher, technical staff of university departments and the student. The pedagogical system model was integrated with the hierarchical structural model of the medical university departments. Implementation of the developed model, in the shortest possible time, made it possible to optimally build the projection of the ground structure by means of MSO365 cloud services to the virtual cloud-oriented learning environment of the medical university and provide distance learning. The developed structure was implemented in the educational process of Zaporizhzhia State Medical University (ZSMU) during the period of COVID – 19 quarantine. The gained experimental experience in the organization of distance learning was used as the basis for the ZSMU mixed form of studying.

Nvestigation of the antimicrobial and antifungal activities of some 1,2,4-triazole derivatives

This article presents the results of the study of the antimicrobial and antifungal properties among 1,2,4-triazole derivatives synthesized at the Department of Physical and Colloidal Chemistry of the Zaporizhzhia State Medical University. Previous studies have established the antimicrobial and antifungal activity of 1,2,4-triazole derivatives. Therefore, it was reasonable to investigate highly effective substances with antimicrobial and antifungal properties among synthesized compounds. In the first stage of our research, acute toxicity prediction was performed. The antimicrobial and antifungal properties were carried out by the method of “serial dilutions” on a liquid nutrient. Forty-seven compounds of the different classes were studied for these types of activities. According to our research, derivatives of 3-amino-1,2,4-triazole showed better performance than 3-thio-1,2.4-triazoles for Staphylococcus aureus and Candida albicans. 5-(1Н-tetrazole-1-іl)methyl-4Н- -1,2,4-triazole-3-yl-1-(5-nitrofuran-2-yl)methanimin (11.6) was showed the greatest antimicrobial and antifungal activity. Deeper research for compound 11.6 was done by diffusion in agar (method of “wells”). Studies have shown that molecule 11.6 showed antimicrobial and antifungal action to the studied test strains at a concentration of 2 μg/ml. Hence, this compound can be developed as a helpful therapeutic agent after establishing its safety pharmacology and toxicity.

COVID-19 pandemic and quarantine-related health disorders in combatants of Ukraine

Here we expand the existing knowledge about disorders associated with the COVID-19-related pandemic and quarantine. We show that the COVID-19-related pandemic and quarantine can reduce the combat effectiveness of the military, provoking a deterioration in the health of combatants and requires hospitalization. Aim. Investigate the impact of the COVID-19 pandemic and quarantine on the health of Ukrainian combatants. Material and methods. A study was conducted between March 12, 2020 and May 22, 2020 at the Department of Psychiatry, Psychotherapy, General and Medical Psychology, Narcology, and Sexology in Zaporizhzhia State Medical University and Zaporizhzhia military hospital (Ukraine). All combatants who were hospitalized during this period were examined. We analyzed medical records, anamnestic data, and performed psychopathological and psychological examinations of all patients. Results. In 56 % of combatants hospitalized during high quarantine measures, the cause of health disorders is negative COVID-19 pandemic and quarantine-related thoughts that provoke stress. These stressful thoughts associated with the COVID-19 pandemic and quarantine provoke the development of hypertensive emergency (57 % vs 18 %), anxiety syndrome (29 %), dissociative [conversion] disorder (7 %), and exacerbate the negative effects of pre-pandemic diseases, namely, hypertension (43 % vs 27 %), somatoform autonomic dysfunction of the cardiovascular system (29 %), panic disorder [episodic paroxysmal anxiety] (7 %), adjustment disorders (7 %), post-traumatic stress disorder (7 %). Conclusions. Preventive measures need to be developed and applied to combatants to prevent their deteriorating health due to the effects of the COVID-19 pandemic and quarantine stress