Young Turkeys Research Articles

The use of reduced intermittent light modes in the cultivation of young replacement turkeys

The use of reduced intermittent light modes in the cultivation of young replacement turkeys

Mycoplasma synoviae dihydrolipoamide dehydrogenase is an immunogenic fibronectin/plasminogen binding protein and a putative adhesin

Mycoplasma synoviae (M. synoviae) is an important avian pathogen that causes arthritis and airsacculitis in young chickens and turkeys. Infection by M. synoviae results in considerable economic losses to the poultry industry worldwide. Cytoadherence is a crucial stage during mycoplasma infection. Dihydrolipoamide dehydrogenase (PdhD) is a flavin-dependent enzyme that is critical for energy metabolism and redox balance. To date, its role in cytoadherence is poorly understood. In this study, recombinant PdhD from M. synoviae (rMSPdhD) was expressed in the supernatant component of E. coli BL21 and rabbit anti-rMSPdhD serum was prepared. rMSPdhD was shown to be an immunogenic protein by immunoblot assays, while the mycoplasmacidal assay revealed that the rabbit anti-rMSPdhD serum had a high complement-dependent mycoplasmacidal rate (88.5 %). Using a suspension immunofluorescence assay and subcellular localization analysis, MSPdhD was shown to be a surface-localized protein distributed in both the cytoplasm and cell membrane of M. synoviae. The enzymatic activity of rMSPdhD was determined by measuring its ability to reduce lipoamide to dihydrolipoamide and convert NADH to NAD+. Using an indirect immunofluorescence assay, rMSPdhD was shown to adhere to DF-1 chicken embryo fibroblast cells. Furthermore, the attachment of M. synoviae to DF-1 cells was significantly inhibited by rabbit anti-rMSPdhD serum. Western blot and ELISA binding assays confirmed that rMSPdhD also bound to fibronectin (Fn) and plasminogen (Plg) in a dose-dependent manner. In conclusion, our data show that MSPdhD is not only a biological enzyme, but also an immunogenic surface-exposed protein that can bind to Fn and Plg as well as adhere to host cells. In addition, we show that rabbit anti-rMSPdhD serum can inhibit the adhesion of M. synoviae to DF-1 cells and has a significant complement-dependent bactericidal activity. Our findings suggest that MSPdhD may be involved in the pathogenesis of M. synoviae.

Effect of Herbal Blend and L-arginine Supplementation on Growth Performance, Intestinal Morphology, and Caecal Microflora of Growing Guinea Fowls

This experiment was carried out to investigate the effect of diet containing herbal blend (HB) of turmeric (Curcuma longa), scent leaf (Ocimum gratissimum), and moringa leaf (Morinda lucida) supplemented with or without L-arginine (L-Arg) on growth performance, intestinal morphology, and caecal microflora of guinea fowls. Three hundred and sixty 28-day-old male guinea fowls were randomly allotted in a completely randomized design to six treatment groups of sixty birds; each treatment group consisted of six replicates of ten birds each. Dietary treatments were laid out in a 3 × 2 factorial arrangement of the basal diet (control), diet containing enrofloxacin (1 g/kg), HB (1 g/kg diet), and each supplemented with or without L-Arg at 1 g/kg. Notwithstanding dietary supplementation with L-Arg, guinea fowls fed the diets with HB, and their counterparts fed the diets with antibiotics had similar weight gain during the growing period. Dietary L-Arg supplementation with HB increased (p<0.05) feed intake. Feed conversion ratio (p<0.05) was improved in guinea fowls fed the diets with HB and their counterparts fed with antibiotic supplemented or not with L-Arg. L-Arg supplementation of the diet with HB resulted in the longest (p<0.05) duodenal villi height and the shortest (p<0.05) duodenal apical width in young turkeys. The caeca content of growing guinea fowls fed the diet with HB supplemented with L-Arg had the least (p<0.05) Clostridium count and the highest (p<0.05) Lactobaccillus count. In conclusion, guinea fowls fed the diet with HB supplemented or not with L-Arg had similar growth performance with those fed with an antibiotic. L-Arg supplementation of the diet with HB resulted in increased caecal Lactobacillus counts of growing birds.

New branch of poultry breeding in the Tyumen region

In many regions of the Russian Federation, the turkey breeding industry is just beginning to develop, which determines the relevance of research at the regional level. The purpose of the research was to analyze the production activity of a new branch of turkey breeding in the Tyumen region. The technology of keeping turkeys of the cross Hybrid Converter under the conditions of LLC “Absolut-Agro” at the Yurginskaya poultry farm has been analyzed in the paper. LLC “Absolut-Agro” is a large and promising poultry enterprise that has a closed production cycle. The cross Hybrid Converter was obtained as a result of crossbreeding two breeds: Bronze broad-chested and White Dutch. The analysis of the rearing of young turkeys from the daily age to slaughter has been carried out. In order to conduct the research 3 groups of turkeys have been formed by the years of rearing – 2017, 2018 and 2019. The dynamics of the growth of young turkeys has been presented, the effectiveness of feed use, the livability of poultry has been analyzed, and the economic effectiveness of rearing has been calculated. In order to determine the effectiveness of feed use, we studied the growth dynamics of young turkeys. During the rearing periods, observations were made on the livability of poultry, the state of health, and the feed consumption. The change in live weight has been determined by weighing the young turkeys every seven days during the rearing period. The group of rearing turkeys in 2019 surpassed the groups of 2017 and 2018 in all indicators, which were due to the enterprise reaching full production capacity in 2018. The poultry raised in 2019 showed the highest production effect. Feed consumption per 1 kg of live weight gain during the rearing period amounted to 2,25 kg in 2017, 2,19 kg in 2018, the lowest feed consumption was in 2019 and amounted to 2,16 kg. Thus, the current state of the turkey breeding industry has just begun its activity in the Tyumen region. In the company LLC “Absolut Agro” the most modern equipment has been installed and created conditions for turkey rearing, which allow improving the production indicators of the enterprise every year and developing the new poultry industry in the region.

Associations between plumage colour and fear behaviour in young Nigerian indigenous turkeys (Meleagris gallopavo)

The effects of plumage colour on different behaviours have been reported in several avian species, but there are only few studies for Nigerian indigenous turkeys (NIT). Fear is a negative affective state that is indicative of suffering and associated with adverse effects on welfare and productivity of livestock species. This study compared fear behaviours in NIT with different plumage colours during early life (first 16 days of life). Seventy-five birds (25 each of white, black and lavender) were used in this study. The three colour phenotypes were derived from the same genetic line. Tonic immobility, emergence, open field, inversion and attention bias tests were done at 7, 9, 12, 14 and 16 days old, respectively. Data were analysed with Kruskal-Wallis tests. The durations of tonic immobility and freezing during the attention bias test were not significantly associated with plumage colour (χ2 =1.7592, df=2, p = 0.42 and χ2 =3.9421, df=2, p = 0.14, respectively). The latency to emerge from a dark box was significantly related to plumage colour (χ2 =8.9229, df=2, p = 0.01), with the shortest emergence latency observed in black NIT. There was a significant plumage colour effect on the duration of ambulation (χ2 =9.6013, df=2, p = 0.01) and a significant effect on the percentage of floor squares explored by the birds in the open field test (χ2 =6.1191, df=2, p = 0.05). The least ambulation time was observed in black NIT (p = 0.02). Lavender turkeys explored fewer floor squares compared to black and white NIT (p = 0.04). The frequency of escape attempts was significantly associated with the plumage colour of the birds (χ2 =11.6810, df=2, p = 0.00). The highest frequency of escape attempts during the open field test was observed in black birds, while there was no significant difference in the frequency of escape attempts of white and lavender birds. There were no plumage colour effects on the duration of preening (χ2 =1.5896, df=2, p = 0.45), wall pecking (χ2 =3.3346, df=2, p = 0.19) and floor pecking (χ2 =1.7483, df=2, p = 0.42) of NIT in the open field test. The results from this study applied to the early life stage. It can be concluded that plumage colour influenced the level of fear in young NIT, with lavender turkeys being the most fearful in three out of the four statistically significant fear indicators (longer emergence duration, lower number of escape attempts and lower percentage of floor squares explored in an open field test), followed by white, whereas black turkeys were least fearful.

Age-Associated Changes in Recombinant H5 Highly Pathogenic and Low Pathogenic Avian Influenza Hemagglutinin Tissue Binding in Domestic Poultry Species.

Simple SummaryWe evaluated differences in avian influenza H5 Hemagglutinin (HA) tissue binding across age groups using recombinant H5 HA (rHA) proteins. Gene sequences from low pathogenic (LPAIV) (A/mallard/MN/410/2000(H5N2) and an a high pathogenic (HPAIV) (A/Northern pintail/Washington/40964/2014(H5N2)) influenza A virus (IAV) were used to generate the rHA proteins. Respiratory and intestinal tracts from chickens, ducks (Mallard, Pekin, Muscovy), and turkeys of different age groups were used to detect rHA binding with protein histochemistry, which was quantified as the median area of binding (MAB) for statistical analysis. Turkeys had significant differences in the HPAIV rHA binding in the respiratory tract, with younger turkeys having higher levels of binding in the lung compared to the older group. In the intestinal tract, younger turkeys had higher levels of binding compared to the older birds. Using LPAIV, only turkeys had overall significant differences in the respiratory tract MAB, with the older birds having higher levels of binding compared to the younger group. We saw no age-related differences in the overall intestinal tract rHA binding. Age-related differences in rHA binding of the LPAIV and HPAIV rHA demonstrated in this study may partially, but not completely, explain differences in host susceptibility to infection observed during avian influenza outbreaks and in experimental infection studies.The 2014 outbreak of clade 2.3.4.4A highly pathogenic avian influenza (HPAI) led to the culling of millions of commercial chickens and turkeys and death of various wild bird species. In this outbreak, older chickens and turkeys were commonly infected, and succumbed to clinical disease compared to younger aged birds such chicken broilers. Some experimental studies using waterfowl species have shown age-related differences in susceptibility to clinical disease with HPAI viruses. Here, we evaluate differences in H5 Hemagglutinin (HA) tissue binding across age groups, using recombinant H5 HA (rHA) proteins generated using gene sequences from low pathogenic (A/mallard/MN/410/2000(H5N2 (LPAIV)) and a HPAIV (A/Northern pintail/Washington/40964/2014(H5N2)) influenza A virus (IAV). Respiratory and intestinal tracts from chickens, ducks (Mallard, Pekin, Muscovy) and turkeys of different age groups were used to detect rHA binding with protein histochemistry, which was quantified as the median area of binding (MAB) used for statistical analysis. There were species and tissue specific differences in the rHA binding among the age groups; however, turkeys had significant differences in the HPAIV rHA binding in the respiratory tract, with younger turkeys having higher levels of binding in the lung compared to the older group. In addition, in the intestinal tract, younger turkeys had higher levels of binding compared to the older birds. Using LPAIV, similar species and tissues, specific differences were seen among the age groups; however, only turkeys had overall significant differences in the respiratory tract MAB, with the older birds having higher levels of binding compared to the younger group. No age-related differences were seen in the overall intestinal tract rHA binding. Age-related differences in rHA binding of the LPAIV and HPAIV demonstrated in this study may partially, but not completely, explain differences in host susceptibility to infection observed during avian influenza outbreaks and in experimental infection studies.

The effect of feed processing of novel unheated, low trypsin inhibitor soybeans on the performance of young female turkeys reared from hatch to 21 days of age

The objective of this study was to evaluate the effects of dietary inclusion of a novel low trypsin inhibitor soybean (LTI) fed as mash or crumbled pellet form on the productive performance and digestibility in turkey poults from hatch to 21 d. A total of 336 Hybrid Converter turkey poults were allocated in groups of 7 to 6 treatments, each with 8 replicate cages. Treatments were arranged as a 3 × 2 factorial with inclusion level of the low trypsin-inhibitor soybean (0, 20, and 40% LTI) and feed form (mash and crumbled pellet). A single batch of feed was mixed for each inclusion level and divided into 2 aliquots: one remaining as mash and the other conditioned at 82°C for approximately 30 s, pelleted and then crumbled. On d 7, 14, and 21 posthatching, BW, and feed intake (FI) were recorded and BW gain (BWG) and feed conversion ratio (FCR) calculated. Excreta samples were collected from d 19 to 21 and pooled by cage. At d 21, intestines were excised, pancreas weights recorded (PRW), and ileal contents collected. There were no interactions (linear, P > 0.05) between LTI inclusion and feed form on BW, BWG, FI, or FCR at d 7 or 21. Increasing LTI resulted in a linear reduction in BW at 7, 14, and 21 d (P < 0.006). Poults fed crumbles were significantly heavier at d 21 than those fed mash feed (P < 0.027) with no interaction of LTI level with feed form. FCR was not significantly greater with increasing LTI. However, poults fed crumbles had a better, lower FCR that those fed mash feed from d 0 to 21 (P < 0.018). There was a significant interaction between feed form and LTI level at 14 d (P < 0.031), but not 7 or 21 d. Pancreatic hypertrophy (PRW) increased linearly with increasing LTI (P < 0.001) with a significant linear interaction with feed form (P < 0.001). Poults fed crumbles had less pancreatic hypertrophy. At 21 d of age, dietary fat digestibility (ALD, %) was linearly reduced with increasing LTI (P < 0.001). However, poults fed crumbles had significantly better fat absorption than poults fed mash (91.2 vs. 85.8%) (P < 0.001), and there was a significant linear interaction between feed form and LTI level (P < 0.001). AMEn was significantly better for the poults fed crumbles compared to mash (3228 vs. 3132 kcal/kg) (P < 0.001), and there was a significant linear interaction between feed form and LTI level (P < 0.001). Based on the results this trial, it is possible to include up to 20% unheated full fat LTI soybeans into poult starter diets after pelleting. Pelleting improves nutrient utilization, allowing for greater incorporation of the LTI soybean in the crumbled diet compared to the mash diet.

Pathogenic evaluation of a turkey coronavirus isolate (TCoV NC1743) in turkey poults for establishing a TCoV disease model

Turkey coronavirus (TCoV) can cause a highly contagious enteric disease in turkeys with severe economic losses in the global turkey industry. To date, no commercial vaccines are available for control of the disease. In the present study, we isolated a field strain (NC1743) of TCoV and evaluated its pathogenicity in specific-pathogen-free (SPF) turkey poults to establish a TCoV disease model. The results showed that the TCoV NC1743 isolate was pathogenic to turkey poults with a minimal infectious dose at 106 EID50/bird. About 50 % of one-day-old SPF turkeys infected with the virus’s minimal infectious dose exhibited typical enteric disease signs and lesions from 6 days post-infection (dpi) to the end of the experiment (21 dpi). In contrast, fewer than 20 % of older turkeys (1- or 2-week-old) infected with the same amount of TCoV displayed enteric disease signs, which disappeared after 15–18 dpi. Although all infected turkeys, regardless of age, shed TCoV, the older turkeys shed less virus than the younger birds, and 50 % of the 2-week-old birds even cleared the virus at 21 dpi. Furthermore, the viral infection caused day-old turkeys more body-weight-gain reduction than older birds. The overall data demonstrated that the TCoV NC1743 isolate is a highly pathogenic strain and younger turkeys are more susceptible to TCoV infection than older birds. Thus, one-day-old turkeys infected with the minimal infectious dose of TCoV NC1743 could be used as a TCoV disease model to study the disease pathogenesis, and the TCoV NC1743 strain could be used as a challenge virus to evaluate a vaccine protective efficacy.

Growth performance, immune status and intestinal fermentative processes of young turkeys fed diet with additive of full fat meals from Tenebrio molitor and Hermetia illucens

Full-fat insect meals were used in young turkeys feeding as feed additives to exert beneficial changes in the intestinal bacterial ecosystem functioning as well as in the immune and antioxidant systems. Two full-fat insect flours obtained from Tenebrio molitor (TM) and Hermetia illucens (HI) insects were added to the base diet (C) in the amount of 3 g/kg and compared to the control and to the effects following common monensin (MON) addition (dietary treatments: C, MON, TM, and HI). Although, the addition of full-fat insect meals to the diet didn't improve performance results such as monensin, it does exerted anti-inflammatory, immunostimulatory and antioxidant changes in turkeys’ blood. The HI and TM dietary treatments caused beneficial reduction in the blood total cholesterol content as well as increase in phosphorus which was not noted in MON treatment. The results indicated that in comparison to the control diet without any additives, the MON, TM and HI additives showed non-selective bacteriostatic properties, without adverse intensification of protein fermentation and pH digesta rise in the caeca of young turkeys. The addition of TM and HI similar as MON reduced the enzymatic activity of caecal microbiota, and decreased caecal concentration of all short-chain fatty acids, except butyric one. The caecal ammonia and dry matter concentration, and pH value of digesta were not affected by dietary treatments.

Use of fly larvae Hermetia illucens in poultry feeding: A review paper

The paper presents the review of scientific publications of world literature on the use of the larvae of black soldier fly ( Hermetia illucens) when feeding poultry. Nowadays, the issue of replacing traditional sources of protein when feeding poultry is very urgent, especially in connection with the global food crisis. Insects are the natural food of most birds; thus, the use of fly larvae for feed production has a biological basis. The research results presented in published works show that there are no negative effects on bird health and meat quality when feeding poultry, quail and other birds. In some cases, the experimental groups of birds gained weight slightly more slowly than the control group. Other reports indicate that birds grew at the same rate as normal birds. The quantity and quality of eggs did not differ significantly, but dietary changes affected the colour of yolks and eggshell. The effect of the addition of live larvae to the diet of young turkeys on the weight characteristics of was studied. Replacing 10% of the daily amount of feed with live Hermetia illucens larvae in the diet of turkeys showed that the daily feed intake and body weight gain of the experimental birds were significantly higher compared to the control groups, which led to a significantly higher body weight of chicks at the age of five weeks (2.19 kg vs. 2.015 kg, respectively) and a significantly lower feed conversion rate. Most researchers agree that replacing protein in poultry feed with insect flour should be partial, in the 15–30% range. Feeding with larvae that have undergone processing – grinding, chitin removing, heat treatment – is more preferable than using whole larvae, since the chitinous membrane makes larvae difficult to be digested in the digestive tract of birds.

DYNAMICS OF INVASION OF YOUNG TURKEY EIMERIA SPP. IN THE PROCESS OF THE TECHNOLOGICAL CYCLE OF THEIR CULTIVATION

Among the different sub-branches of poultry farming, turkey breeding is especially attractive due to the intensive production technology and biological characteristics obtained from turkey products. Based on the experience of turkey farming and analysis of the literature, it is obvious that with the development of industrial turkey breeding, a problem arises associated with the pathology of this species of birds, the study of which has not yet been given due attention. To establish the infestation with intestinal parasitic protozoa of young turkeys in the form of the disease Eimeria spp. in 2020 from a specialized turkey breeding farm of the Penza region of Central Russia, samples of feces were examined for the presence of invasive elements. For research coproscopic methods of Fülleborn and Darling were used. Young turkeys of different ages were examined for eimeriosis by the method of intravital coproscopy. On the farm of the Penza region, turkeys were examined from 7 days of age until the full completion of the technological cycle every 14 days by examining at least 20 fresh samples of feces. The study of the dynamics of the invasion of young turkeys with eimeria in the process of the technological cycle of their rearing showed that the greatest infestation of males fell on 35–49 days of age, EI – 30–45%. In young females, the invasion of eimeria was noticeably lower, the peak of infection was at 35–49–63 days of age, EI – 5–30%. The drug Monensin used by us as a coccidiostatic agent in the recommended dose and scheme provided satisfactory efficacy, preventing the clinical manifestation of coccidiosis in young turkeys during the technological cycle of their rearing. Based on the real epizootic situation at poultry farms, it is necessary to carry out comprehensive therapeutic and prophylactic measures against the noted parasitoses, taking into account the exo- and endogenous stages of parasite development.

The effect of dietary full-fat Hermetia illucens larvae meal on gut physiology and growth performance in young turkeys

The aim of this study was to determine the efficacy of full-fat Hermetia illucens larvae meal (HM) as a protein source in diets for the youngest turkey poults, based on growth performance and parameters of intestinal function, i.e. immune status and fermentation processes in the gut. The experiment was carried on 432 one-day-old male Hybrid Converter turkeys. The birds were randomly divided into four dietary treatment groups, with twelve replicates of nine birds each. During a four-week feeding period, the HM content of turkey diets was 50, 100 and 150 g/kg in groups HM50, HM100 and HM150, respectively. The highest HM content in the experimental diet HM150, limited by high crude fat content, replaced 1/3 of soybean meal (SBM) (295 vs. 429 g/kg) in the control diet HM0. The inclusion of dietary HM resulted in a linear increase in the dry matter (DM) content and viscosity of small intestinal contents. The highest inclusion rate of HM did not affect IgA levels, but it increased IgY levels in the small intestinal tissue compared with the control treatment. At the same time, dietary HM exerted anti-inflammatory effect by decreasing TNFα and increasing IL-6 levels in small intestinal tissue (linear and quadratic response, respectively). The dietary inclusion of HM increased the activity of selected microbial enzymes (α-galactosidase, β-glucosidase and β-glucuronidase) and butyric acid concentration in the cecal digesta. Increasing inclusion levels of HM did not affect the final body weight (BW) or body weight gain (BWG) of birds, but induced a linear decrease in the feed conversion ratio (FCR). The results of this study indicate that HM can be included in diets for young turkeys at up to 150 g/kg without compromising the immune status or growth performance of birds, intestinal development and function.

Infection with Avian Coronaviruses: A recurring problem in turkeys

Turkey coronavirus (TCoV) is a Gammacoronavirus causing acute contagious enteritis in young turkeys, leading to impaired growth, low feed conversion, and increased mortality. The TCoV infections, in association/combination with other enteropathogenic viruses, bacteria &amp; protozoa, are associated with poult enteritis-mortality syndrome (PEMS) in turkeys of 1-4 weeks age. In this review, classification &amp; genotyping of TCoV, the implications of its recombination, and challenges to develop efficient vaccines against it are discussed. Though TCoV is monophyletic with infectious bronchitis virus (IBV) with a sequence similarity of ≥86, however a classification scheme gathering all avian coronaviruses (ACoVs) is not established. Based on the N gene, ACoVs are classified into five clades. Clades 1 &amp; 2 (chickens), Clade 3 (pigeon) Clade 4 (duck), and Clade 5 (goose). The Spike (S) gene of ACoVs has shown exceptional lability of being easily switched with multiple recombination events suggesting that TCoV may be an IBV recombinant. Recombination events altered the pathogenicity, host specificity, and tissue tropism of TCoVs. Attempts to develop attenuated, inactivated, DNA, and virus-vectored vaccines are ongoing. Experimentally, the attenuated TCoV strains induced strong humoral and cellular immune responses and completely protected against the homologous challenge but not heterologous TCoV challenge. Meanwhile, genetically engineered vaccines, either DNA or virus vectored vaccines, are limited with either late induction of a protective immune response and/or inability of the elicited antibody to neutralize virus infection and protect against virus challenge. Future research should focus on improving vaccine efficiency against TCoVs by developing more immunogenic vaccines, determining the appropriate dosing regimens, and include potent adjuvants.

Антиоксидантные свойства феруловой кислоты и основные результаты их реализации в промышленной инкубации индеек

The presented article presented data confirming the antioxidant properties of ferulic acid, which is associated with inhibition of the intensity of lipid peroxidation in the body of day-old turkeys, expressed in a decrease in the concentration of its cytotoxic products formed at different stages of the peroxide cascade: isolated double bonds (IDB) by 1,2 times, diene conjugates (DC) 1,6 times (p&lt;0,001,.1,1), triene conjugates (TC) and oxodienic conjugates (ODC) 1,4 times, Schiff bases (SB) 12,02 times compared to the control. This helped to optimize the central metabolic processes, in particular carbohydrate metabolism. The paper analyzes these changes, presents and explains their relationship with the increase in quantitative and qualitative indicators of young turkeys at the early stages of ontogenesis. Thus, the superiority of the experimental group over the control of hatching turkey poults was 0,01% (p&lt;6,66), the hatchability of eggs was 8,23% with a tendency to an increase in live weight of individuals of day-old age by 0,5%, the score according to the «Pasgar» scales and «Optistart» by 0,05 (p&lt;1,0) and 0,001 points (p&lt;XNUMX), respectively.

Antioxidant Status and Liver Function of Young Turkeys Receiving a Diet with Full-Fat Insect Meal from Hermetia illucens.

Simple SummaryInsects can be used as an alternative source of protein and fat in poultry feed. To date, in most research, the administration of invertebrates as a replacement for soybean meal in chicken diets has produced satisfactory results. We hypothesized that full-fat insect meal from Hermetia illucens (HI) larvae can also be an acceptable source of protein and energy in the diet of young turkeys, and at the same time can improve their antioxidant status and metabolism. Our research showed that the level of HI meal in the diet of turkeys should not exceed 5%. The use of a higher level of HI than 5% in the diet of young turkeys has a negative effect on lipid metabolism, lipid oxidation and fat deposition in the liver.We hypothesized that full-fat insect meal from Hermetia illucens (HI) larvae can be an acceptable source of protein and energy in the diet of young turkeys, in an amount adapted to the nutritional needs of these birds, and at the same time can improve their antioxidant status and metabolism. The turkeys were fed a control diet (HI0) without the insect meal, and three diets with increasing HI content of 5%, 10% and 15% (treatments HI5, HI10 and HI15, respectively). The use of 10% or 15% HI in the diet of young turkeys, while beneficially raising levels of P, Fe and Hb, has a negative effect on lipid metabolism, increasing TC levels, lipid oxidation, and fat deposition in the liver. The inclusion of 5% HI in the diet of young turkeys has no adverse effect on the lipid status and histology of the liver, but it does not improve antioxidant status. To conclude, the level of HI meal in the diet of turkeys should not exceed 5%. However, as similar studies on turkeys have not yet been published, overly general conclusions should not be drawn from the results of the present study, and further research is necessary.

The Immunological Reactivity of Turkeys of Different Genotypes on the Action of Environmental Factors

The Immunological Reactivity of Turkeys of Different Genotypes on the Action of Environmental Factors

FR01-11 KIDNEYS IN THE KITCHEN, TESTES AT THE TABLE: ANCIENT AND MODERN GENITOURINARY DELICACIES

You have accessJournal of UrologyHistory of Urology Forum I (FR01)1 Apr 2020FR01-11 KIDNEYS IN THE KITCHEN, TESTES AT THE TABLE: ANCIENT AND MODERN GENITOURINARY DELICACIES Alexandra L. Tabakin*, Arnav Srivastava, and Ephrem O. Olweny Alexandra L. Tabakin*Alexandra L. Tabakin* More articles by this author , Arnav SrivastavaArnav Srivastava More articles by this author , and Ephrem O. OlwenyEphrem O. Olweny More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000850.011AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: People have eaten genitourinary offal, or non-muscle meat, since biblical times. We present a brief history of the use of kidneys and testes as food in both ancient and contemporary culture. METHODS: Literature review of primary and secondary sources regarding consumption of kidneys and testes was performed. RESULTS: Usage of kidneys as food was first described in Deuteronomy 32:14 when the Lord fed Jacob “fat of kidneys of wheat”. Centuries later, ancient Greek physician Galen (130-200 AD) described kidneys as fattening and indigestible. He commented that most testes are “difficult to digest;” however, those of a young pig or rooster are “very pleasant and give the body excellent nourishment”. Succeeding Greek physician Oribasius, as well as Byzantine physicians Aetius of Amida and Paulus Aeginita, documented similar opinions in their manuscripts. In 8th century Greece, Olympic athletes ate raw testes to gain muscle and strength in preparation for competitions. In ancient India, Vātsyāyana, in his text Kama Sutra, recommended eating goat or ram testicle mixed with milk and sugar to boost libido. The idea that eating testes may improve strength, libido, and potency still persists in some regions. In China, testes served with penises, are purported to improve impotence if eaten regularly. In American agriculture, harvesting the testes of young lambs, calves, and turkeys has become a yearly spring tradition in many rural areas, as it is custom to not let any part of the animal go to waste. Annual “testicle festivals” are held throughout the United States where attendees eat various animal testes including renowned “Rocky Mountain Oysters”, deep fried and breaded bull testes. Kidneys, regarded for high B12 and iron content, were first incorporated into the American diet in the early 1940s after a meat shortage resulted from shipping meats overseas to troops fighting World War II. Americans incorporated offal into their diets, the consumption of which rose by 50% by 1955. Kidney meat consumption in the US has since declined but is still used commonly in Asia and Europe in stews and “steak and kidney pie”. Moreover, some integrative medicine practitioners recommend glandular orchic tablets and grass-fed beef kidney tablets, pill preparations of animal testes and kidneys, respectively, for low libido and improving kidney health. CONCLUSIONS: Many cultures revere kidney and testis meat as a delicacy. The perceived health benefits of these organ meats continue to influence people today. Source of Funding: None © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e287-e288 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Alexandra L. Tabakin* More articles by this author Arnav Srivastava More articles by this author Ephrem O. Olweny More articles by this author Expand All Advertisement PDF downloadLoading ...

Molecular Detection of Salmonella enterica subsp. arizonae by Quantitative PCR.

Salmonella enterica subspecies arizonae (subspecies IIIa) is most frequently associated with reptiles but is also a bacterial pathogen of poultry, primarily of young turkeys where it induces septicemia, neurologic signs, and increased mortality. Arizonosis clinical cases in broiler chickens have recently been documented in the United States, driving the development of a rapid, molecular-based diagnostic for this subspecies. S. enterica subsp. arizonae is a genetically distinct subgroup of S. enterica, primarily diagnosed through culture followed by serotyping or biochemical identification, which are costly in both time and laboratory resources. Real-time/quantitative PCR offers rapid and sensitive detection of Salmonella sp. in laboratory and diagnostic samples; however, no such methodology exists to differentiate S. enterica subsp. arizonae from other Salmonella sp. In this study, we designed a quantitative PCR assay for S. enterica subsp. arizonae. The assay is able to differentiate S. enterica subsp. arizonae from other S. enterica subspecies, including S. enterica subsp. diarizonae (IIIb), and other non-Salmonella bacteria. Validation, including 56 different S. enterica subsp. arizonae serovars, demonstrated 100% sensitivity and 100% specificity. This assay provides a rapid diagnostic option for suspected cases of arizonosis in poultry.

Comparative aspects of phytase and xylanase effects on performance, mineral digestibility, and ileal phytate degradation in broilers and turkeys

Two experiments were performed, using broilers or turkeys, each utilizing a 3 × 2 factorial arrangement, to compare their response to phytase and xylanase supplementation with growth performance, nutrient digestibility, and ileal phytate degradation as response criteria. For both experiments, 960 Ross 308 or 960 BUT 10 (0-day-old) birds were allocated to 6 treatments: (1) control diet, containing phytase at 500 FTU/kg; (2) the control diet with xylanase (16,000 BXU/kg); (3) the control diet supplemented on top with phytase (1,500 FTU/kg); (4) diet supplemented with 1,500 FTU/kg phytase and xylanase (16,000 BXU/kg); (5) the control diet supplemented with phytase (3,000 FTU/kg); and (6) diet supplemented with 3,000 FTU/kg phytase and xylanase (16,000 BXU/kg). Each treatment had 8 replicates of 20 birds each. Water and diets based on wheat, soybean meal, oilseed rape meal, and barley were available ad libitum. Body weight gain and feed intake were measured from 0 to 28 D, and feed conversion ratio (FCR) corrected for mortality was calculated. Ileal digestibility for dry matter and minerals on day 7 and 28 were analyzed in addition to levels of inositol phosphate esters (InsP6-3) and myo-inositol. Statistical comparisons were performed using ANOVA. Xylanase supplementation improved 28D FCR in broilers and turkeys. Increasing doses of phytase reduced FI and improved FCR only in broilers. In broilers, the age × phytase interaction for phosphorous digestibility showed that increasing phytase dose was more visible on day 7, than on day 28. Mineral digestibility was lower in 28-day-old turkey compared with 7-day-old turkey. InsP6 disappearance increased with increasing phytase levels in both species, with lower levels analyzed in turkeys. InsP6 disappearance was greater in younger turkeys (day 7 compared with day 28). In conclusion, although broilers and turkeys shared several similarities in their growth and nutrient utilization responses, the outcomes of the 2 trials also differed in many aspects. Whether this is because of difference in diets (InsP or Ca level) or differences between species needs further investigation.

Biochemical status of turkeys when fed with a complex nanoadditive

The positive effect of a complex additive based on nanozeolite and soy оkara on the biochemical status and anabolic processes in turkeys was established. Tests were carried out in the Ulyanovsk region at the facilities of a farm for young turkeys of the breed “Hybrid Grade Maker”. We formed two groups using the method of analogs: control and experimental groups of 10 birds each. The supplement was added to the diet in the amount of 100 g/head/day. The subject of the study was the blood of birds, which was taken from the axillary vein before morning feeding. Under the influence of the supplement, an increase in anabolic processes in the body of turkeys was revealed. An increase in albumin by 7.38% p&lt;0.05, creatinine by 19.32% p&lt;0.05 with a decrease in nitrogenous products was found. It is confirmed by an increase in the average daily weight gains of turkeys by 13-18.3%. There was a decrease in the activity of AST aminotransferases. Decreased of the products nitrous metabolism is revealed: urea concentration per uric acid. Improved feather cover of birds and reduced cannibalism among birds.