Young Bamboo Research Articles

A Review of the Nutritional Composition, Storage Challenges, Processing Technology and Widespread Use of Bamboo Shoots

Bamboo shoots, as the young bamboo stems, are rich in protein, fiber, vitamins, and minerals, as well as many bioactive substances beneficial to health, and are gaining in importance worldwide as a healthy food and dietary supplement. However, fresh bamboo shoots lignify rapidly after harvesting and contain cyanogenic glycosides, limiting the safe and healthy consumption of bamboo shoots. To this end, based on the changes in nutritional composition and the physiological properties of fresh and post-harvest bamboo shoots, factors affecting the preservation of post-harvest bamboo shoots are emphasized, including a series of physical and chemical regimes and various processing methods for post-harvest preservation. Furthermore, a systematic biorefinery approach for using bamboo shoot processing residue to prepare value-added products is also discussed. Finally, the article also discusses issues related to sustainable development, safeguarding food security, and addressing potential health impacts in order to provide a scientific basis for researchers to further develop and increase the added value of bamboo shoots.

Stoichiometric Homeostasis of N and P in the Leaves of Different-Aged Phyllostachys edulis after Bamboo Forest Expansion in Subtropical China

Stoichiometric homeostasis is an important mechanism in maintaining ecosystem structure, function, and stability. Phyllostachys edulis (moso bamboo) is a typical clone plant, forming pure bamboo forests or bamboo–wood mixed forests by expanding rhizomes around. Studying the stoichiometric homeostasis characteristics of moso bamboo at different ages after expansion contributes to a deeper understanding of the stability of bamboo forest ecosystems, and is of great significance for expanding the research scope of ecological stoichiometry. Based on the stoichiometric internal stability theory, the nitrogen (N) and phosphorus (P) elements in the soil and plants of typical moso bamboo forests in Tianbaoyan National Nature Reserve of Fujian Province were determined, and the internal stability index (H) of bamboo leaves of different ages (I-du, II-du, III-du, and IV-du bamboos) was calculated. The results showed that the dependence of moso bamboo on soil nutrients and the ability of moso bamboo to regulate nutrient elements were both significantly affected by the plant’s age. Under the condition of the same soil nutrients (N, P), the content of N and P in bamboo leaves decreased significantly with the increase in bamboo age. The limiting effect of phosphorus on the growth and development of moso bamboo was greater than that of nitrogen, and the limiting effect of phosphorus on aged bamboo was greater than that of young bamboo. The stoichiometric internal stability index of N and P in bamboo leaves is HN:P > HN > HP, which means that the internal stability of moso bamboo is closely related to the limiting elements. Therefore, the regulation ability of the internal stability of moso bamboo of different ages makes it grow well in the changeable environment, has stronger adaptability and competitiveness, and the leaf internal stability of I-du bamboo was higher than that of other ages, which may be one of the reasons for its successful expansion to form a stable bamboo stand structure.

High nutrient utilization and resorption efficiency promote bamboo expansion and invasion

Bamboos are fast-growing, aggressively-spreading, and invasive woody clonal species that often encroach upon adjacent tree plantations, forming bamboo–tree mixed plantations. However, the effects of bamboo invasion on leaf carbon (C) assimilation, and nitrogen (N) and phosphorus (P) utilization characteristics remains unclear. We selected four different stands of Pleioblastus amarus invading Chinese fir (Cunninghamia lanceolata) plantations to investigate the concentrations, stoichiometry, and allometric growth relationships of mature and withered leaves of young and old bamboos, analyzing N and P utilization and resorption patterns. The stand type, bamboo age, and their interaction affected the concentrations, stoichiometry and allometric growth patterns of leaf C, N, and P in both old and young bamboos, as well as the N and P resorption efficiency. Bamboo invasion into Chinese fir plantations decreased leaf C, N, and P concentrations, C:N and C:P ratios, N and P resorption efficiency, and allometric growth exponents among leaf C, N, and P, while it only slightly altered N:P ratios. PLS-PM analysis revealed that bamboo invasion negatively impacted leaf C, N, and P concentrations, as well as N and P utilization and resorption. The results indicate that high N and P utilization and resorption efficiency, along with the mutual sharing of C, N, and P among bamboos in interface zones, promote continuous bamboo expansion and invasion. Collectively, these findings highlight the significance of N and P utilization and resorption in bamboo expansion and invasion and provide valuable guidance for the establishment of mixed stands and the ecological management of bamboo forests.

Assessment of Density and Anatomical Features of Young and Old Bambusa vulgaris (Schrad. ex J.C. Wendl.) Culm Heights as Sustainable Structural Material in Ghana

This study sought to assess the density and anatomical features of young and old Bambusa vulgaris Schrad. ex JC Wendl. culm heights and, therefore, evaluate their influence on the utilization potential of the culm as a sustainable structural material. Bambusa vulgaris culm of 2-year and 4-year-old were harvested and prepared to the required length of 6 meters for the study. Basic density and anatomical features were carried out. The results showed that the basic density of old bamboo ranges from 652.91 kg/m³ to 729.06 kg/m³, while the young bamboo ranges from 342.33 kg/m³ to 509.52 kg/m³. The vascular bundle arrangement was found to be in Type III and Type IV for the culm heights of both young and old bamboo. The average vascular bundle radial diameter ranged from 761.13 μm to 890.78 μm for the old culm and 727.16 μm to 844.83 μm for the young culm. The average vascular bundle tangential diameter ranged from 499.44 μm to 533.49 μm for the old culm and 425.56 μm to 483.56 μm for the young culm. The radial diameter of the metaxylem vessel of the old culm ranged from 216.81 μm to 54.74 μm, while 214.23 μm to 73.86 μm were for the young culm. Parenchyma tissues ranged from 35%– to 70% for the old bamboo culm and 45% to 75% for the young bamboo culm. Vessel proportion ranged from 6%–12% for the old culm while the young culm ranged from 5%–11%. Fiber proportion ranged from 22% to 54% for the old culm and 19% to 44% for the young culm. Old bamboo had better vascular bundle properties than that of young bamboo. The density is significantly correlated positively to the anatomical properties. Old bamboo is more suitable to be used as the engineered composite material due to its better anatomical features across the culm wall thickness.

Perahu dan Rumah Gadang

The environment of Lake Singkarak provides natural resources to be utilized by human around it by providing facilities that support their lives. In the water area, of course, boats are needed as a mode of transportation for fishermen. Likewise, markers of settled life in the form of settlements with traditional buildings including ‘Rumah Gadang’. This paper uses qualitative methods with inductive reasoning flows. Data was collected on primary (through observation, observation of object and environment, in-depth interviews) and secondary data. The steps in qualitative research are data collection, analysis, and data synthesis. The purpose of writing is to find out about the sustainability of wood culture on the shores of Lake Singkarak and the local wisdom of the community. The change in manufacture and utilization from dugout canoes to plank boats is an adaptation strategy to the scarcity of sources of Surian wood tree materials. The developed buildings and ornaments also depict the complexion of maritime life (in the lake environment). Nature is a source of inspiration in its motifs and coloring, such as the motif of ‘pucuak rabuang’ (young bamboo), flowers, leaves, fish, and the Dutch royal crown. This is a form of local wisdom of the community in adapting to environmental conditions.

Pemanfaatan Sumber Daya Alam Kabupaten Lebong: Sumber Daya Pangan Rebung Bambu sebagai Bahan Baku Utama Pembuatan Lemea (Makanan Tradisional Kabupaten Lebong)

This research aimed to determine the use of natural resources as food in Lebong Regency which can later be used as a learning resource for students in science learning. This research uses a survey method conducted in Lebong Regency, Bengkulu Province. The data collection technique used was interviews with the people of Lebong Regency and the Central Statistics Agency (BPS) of Lebong Regency regarding natural resources and their use. Data obtained from interviews were analyzed descriptively. The results of the research show that the people of Lebong Regency utilize natural resources, namely bamboo which is used as the main raw material in making lemea (a traditional food of the Rejang Tribe in Lebong Regency). The bamboo used in the process of making Lemea is a type of ship bamboo. The part of bamboo used in the process of making Lemea is bamboo shoots (young bamboo shoots). Lemea is a typical food of the Rejang tribe in Bengkulu Province where the main ingredient of lemea is chopped fermented bamboo shoots (young bamboo). The ingredients for the lemea mixture vary from region to region, some use river fish, red chilies, bay leaves and lemongrass. The science content that students can learn in this Lemea product is fermentation in biotechnology material. Keywords: Bamboo, Lebong, Lemea, Bamboo Shoots, Natural Resources

Effect of An Additional Amount of Fish and Fermentation Time on The Sensory Properties of The Lemea

Lemea, a traditional fermented food of the Rejang people in Bengkulu, is made from young bamboo and fish. The fish commonly used in the making Lemea is Mozambique tilapia or Mujair (Oreochromis mossambicus). The fermentation process typically lasts between 3 to 7 days. This study aimed to assess the sensory characteristics, including taste, color, texture, and aroma, of Lemea, based on the preferences of panelists. The variations examined were fermentation duration, and the amount of Mujair fish added. The research followed a completely randomized design, incorporating three fermentation durations (24, 96, and 144 hours) and three fish quantities (250 grams, 500 grams, and 750 grams). Sensory evaluations were conducted on the fermented samples, encompassing taste, aroma, texture, and color. The data revealed that the most favored taste and texture of Lemea were achieved with 500 grams of fish and a fermentation duration of 144 hours (J2W3). Likewise, the preferred aroma and color were observed in the sample with 750 grams of fish and 24 hours of fermentation (J3W1). The data analysis indicated that the sample with 500 grams of fish and a fermentation duration of 144 hours (J2W3) exhibited the most favorable sensory characteristics (taste, aroma, texture, and color). Thus, the quantity of fish added and the duration of fermentation influenced the sensory attributes of Lemea.

The “Oxygen Sink” of Bamboo Shoots Regulates and Guarantees the Oxygen Supply for Aerobic Respiration

The amazingly rapid growth of bamboo shoots requires strong respiration and provides a large amount of energy and intermediate metabolites. Strong aerobic respiration requires a large amount of O2. This raises the following question: What is the source and mechanism of O2 supply to meet aerobic respiration? However, currently, this remains unknown. The underground buds (US), the 2-m-high overground buds (AS), and the 8-m-high growth arrest buds (HS) of bamboo (Phyllostachys prominens) were collected to represent their different stages of growth and development. The fifth bamboo shoot node at each stage was sealed by two membranes, and treated in a polyethylene zip-lock bag filled with air (21% O2 + 79% N2) and nitrogen (100% N2) for 1.5 h. The concentrations of free O2 and CO2 in the shoot cavities in polyethylene zip-lock bags, and the ethanol content in the shoot body before and after treatment were determined. In addition, the photosynthetic rates of the fifth bamboo internodes of 1 y/o, 2 y/o and 3 y/o bamboo in the field were measured. The results indicated that: (1) When treated with air and nitrogen, US, AS and HS mainly exhibited aerobic respiration, and there was almost no anaerobic respiration; (2) When treated with air, 59.66%, 54.47% and 45.84% of the O2 in the aerobic respiration of US, AS and HS came from the polyethylene zip-lock bag, 0.06%, 0.57% and 0.650% came from the shoot cavity, but 40.28%, 44.96% and 53.51% of the O2 was of an unknown source; (3) Treated by nitrogen, 0.19%, 4.71% and 4.79% of O2 in aerobic respiration of US, AS and HS came from shoot cavity, while the other 99.808%, 95.290% and 95.21% of O2 came from unknown sources; and (4) The photosynthesis of the fifth internodes of 1 y/o, 2 y/o and 3 y/o bamboo generated little oxygen that could not absolutely meet the huge O2 supply for aerobic respiration. It was concluded that the respiration of P. prominens shoots in its different growth and development stages was dominated by aerobic respiration. O2 supply pathways were mainly via the sheath stomata; however, there was little absorption from dissolved O2 in the soil water and little supply produced by shoot/stem photosynthesis. It was found that the large supply of oxygen in the aerobic respiration of bamboo shoots and young bamboo was of an unknown source under air treatment and nitrogen treatment, i.e., 40.28%–53.51% and 95.21%–99.81% of oxygen in the aerobic respiration of bamboo shoots and young bamboo was of unknown origin, respectively. Therefore, we proposed that bamboo shoots may exhibit the phenomenon of acting as an “oxygen sink”, which can provide a large amount of O2 from unknown sources to ensure the rapid growth of bamboo shoots and young bamboo.

Keripik Rebung :Kreativitas Mahasiswa PGSD Sebagai Upaya Untuk Membentuk Jiwa Enterpreneur

Bamboo shoots are young shoots or saplings that grow from bamboo roots. Bamboo shoots are also known as young bamboo. Bamboo shoots include vegetables that are liked by many people. Bamboo shoot production in Indonesia is also very abundant. The purpose of this activity is to increase the creativity of PGSD students as an effort to form an entrepreneurial spirit by making bamboo shoot chips. This service utilizes bamboo shoots into processed food ingredients that are rich in water, fiber, potassium, protein, fat and carbohydrates in the form of bamboo shoot chips. This activity trains students to make observations, environmental research, and also market research to find out about the availability of raw materials and analyze market share. This activity also trains students to carry out the pre-production, production, packaging, online and offline marketing stages as well as evaluation of the products produced. The result of this activity is the growth of an entrepreneurial spirit in PGSD students by making works in the form of bamboo shoot chips. Student creativity is reflected in the many flavors as an innovation to make bamboo shoot chips more attractive. The flavors that were innovated by PGSD students were balado, cheese, roasted corn, extra spicy and original.

Application of magnetic resonance imaging (MRI) technology in the characterization of microstructure and moisture content of young Moso bamboo.

Bamboo nodes play a key role in the hollow structure and the rapid growth of bamboo culm. Studying on the anatomical structure of bamboo is helpful to understand its growth mechanism. Taking the noninvasive, high-resolution and accurate technical advantages of magnetic resonance imaging (MRI), we conducted cross-sectional high-resolution MRI scanning on the tip of young Moso bamboo culm (removed shoot sheath) and extracted the gray value of the MRIs by using MATLAB software to explore the differences of water distribution in nodes, proximal nodes, and internodes. The results showed that numerous vascular bundles were repeatedly twisted and rotated horizontally at the nodal diaphragms and inner wall near the nodal diaphragms of the young bamboo, forming an intricate and highly connected network. The structure protected important tissues from mechanical stress by allocating axial loads, and enabled to laterally transport water and nutrients, which was an important basis for the rapid growth of Moso bamboo in relatively short term. The signal value (also known as brightness value) of MRIs indicated that water content of vascular bundles in young bamboo culm was much higher than that of surrounding parenchyma tissues. The mean value and standard deviation of water content between pixels of internodes were significantly higher than that of nodes, and the values of that in the proximal nodes were intermediate. The development of MRI would play a significant role in the studies of bamboo anatomy, physiology, and biochemistry.

Unequal nitrogen translocation pattern caused by clonal integration between connected ramets ensures necessary nitrogen supply for young Moso bamboo growth

The rapid growth of young Moso bamboo ( Phyllostachys edulis ), a giant woody clonal plant, depends on consistent nutrient supply through clonal integration. However, the effects of clonal integration on nutrient translocation and allocation in Moso bamboo remain unclear. We established an in situ study model unit consisting of a young bamboo ramet, a mature bamboo ramet, and an interconnected rhizome to study nitrogen (N) translocation and allocation using the 15 N isotope labeling method. The results showed 15 N translocation was bidirectional between interconnected Moso bamboo ramets; however, more 15 N was translocated from the mature to the young ramet than vice versa in winter. The N translocation rate within the donor ramet was faster when the young was donor ramet, and N translocation rate from the donor to the recipient ramet was faster when young bamboo was the recipient ramet. Demand-driven source-sink relationships between connected ramets determined the net flow direction and speed of N translocation. Therefore, an “unequal N translocation pattern between the young and connected mature ramets” was proposed, in which N was preferentially translocated from the mature to the young ramet to meet its growth demand in winter. Our findings provide new insights into the underlying mechanism of Moso bamboo growth and N clonal integration network in Moso bamboo forests and could serve as a guide for precision fertilization in forest management. • N translocation was bidirectional with net flow from the mature to the young ramet. • N translocation within the ramet was faster when the young was donor or recipient ramet. • Demand-driven source-sink relationships determined net N flow direction and speed. • Unequal N translocation between young and mature ramets ensures young ramet growth.

Effects of Extraction Methods on Phenolic Content in the Young Bamboo Culm Extracts of Bambusa beecheyana Munro.

Nowadays, many studies focus on the potential of bamboo as a source of bioactive compounds and natural antioxidants for nutraceutical, pharmaceutical, and food sources. This study is a pioneering effort to determine the total phenolic content, total flavonoid content and free radical scavenging activity, as well as the phenolic identification and quantification of Bambusa beecheyana. The study was conducted by using ethanol, methanol, and water for solvent extraction by applying cold maceration, Soxhlet, and ultrasonic-assisted extraction techniques. The results showed that Soxhlet and ultrasonic-assisted Bambusa beecheyana culm extracts had an increase in the extract’s dry yield (1.13–8.81%) but a constant p-coumaric acid (4) content (0.00035 mg/g) as compared to the extracts from the cold maceration. The ultrasonic-assisted extraction method required only a small amount (250 mL) of solvent to extract the bamboo culms. A significant amount of total phenolics (107.65 ± 0.01 mg GAE/g) and flavonoids (43.89 ± 0.05 mg QE/g) were found in the Soxhlet methanol culm extract. The extract also possessed the most potent antioxidant activity with an IC50 value of 40.43 µg/mL as compared to the positive control, ascorbic acid. The UHPLC–ESI–MS/MS analysis was carried out on the Soxhlet methanol extract, ultrasonic-assisted extract at 40 min, and cold methanol extract. The analysis resulted in the putative identification of a total of five phenolics containing cinnamic acid derivatives. The two cinnamic acid derivatives, p-coumaric acid (4) and 4-methoxycinnamic acid (5), were then used as markers to quantify the concentration of both compounds in all the extracts. Both compounds were not found in the water extracts. These results revealed that the extract from Soxhlet methanol of Bambusa beecheyana could be a potential botanical source of natural antioxidants. This study provides an important chemical composition database for further preclinical research on Bambusa beecheyana.

Biochar Amendment Alters the Nutrient-Use Strategy of Moso Bamboo Under N Additions.

Nutrient resorption can affect plant growth, litter decomposition, and nutrient cycling. Although the effects of nitrogen (N) and biochar fertilizers on soil nutrient concentrations and plant nutrient uptake have been studied, an understanding of how combined applications of N and biochar affect plant nutrient resorption in plantations is lacking. In this study, we applied N (0, 30, 60, and 90 kg N ha−1 yr−1 defined as N0, N30, N60, and N90, respectively) and biochar (0, 20, and 40 t biochar ha−1 defined as BC0, BC20, and BC40, respectively) to the soil of a Moso bamboo plantation. We investigated the effects of these treatments on N and phosphorus (P) resorption by young and mature bamboo plants, as well as the relationships between nutrient resorption and leaf and soil nutrient concentrations. Young bamboo showed significantly greater foliar N resorption efficiency (NRE) and P resorption efficiency (PRE) than mature bamboo. N addition alone significantly increased the N resorption proficiency (NRP) and P resorption proficiency (PRP) but significantly decreased the NRE and PRE of both young and mature bamboo. In both the N-free and N-addition treatments, biochar amendments significantly reduced the foliar NRE and PRE of young bamboo but had the opposite effect on mature bamboo. Foliar NRE and PRE were significantly negatively correlated with fresh leaf N and P concentrations and soil total P concentration but significantly positively correlated with soil pH. Our findings suggest that N addition inhibits plant nutrient resorption and alters the nutrient-use strategy of young and mature bamboo from “conservative consumption” to “resource spending.” Furthermore, biochar amendment enhanced the negative effect of N addition on nutrient resorption in young bamboo but reduced the negative effect on that of mature bamboo under N-addition treatments. This study provides new insights into the combined effects of N and biochar on the nutrient resorption of Moso bamboo and may assist in improving fertilization strategies in Moso bamboo plantations.

Chemical contents and thermal stability of Madu bamboo (Gigantochloa albociliata) for natural-bonded fiber composites

The objective of this study was to evaluate the chemical contents and thermal stability of young (3 years old or less) and matured (more than 5 years old) Madu Bamboo (Gigantochloa albociliata). The comprehensive knowledge of these properties will help to promote the uses of this bamboo’s fiber for natural-bonded composite products. The different age levels of bamboo can be an indicator in evaluating the significant difference of the chemical contents and thermal stability of bamboo. The chemical contents were determined in accordance to the standard outline in Technical Association of Pulp and Paper Industry (TAPPI) test method, while thermal stability was conducted in accordance to Shimadzu TGA 50 analyzer. The culm exhibited different chemical contents between young and old age, in which, young bamboo showed higher hot water extractives and ash contents compared to mature bamboo. On the other hand, matured bamboo contained higher alcohol-toluene extractives, holocellulose, a-cellulose and lignin than young ones. Hence, more resin and possible wood gum in matured bamboo that is beneficially in natural bonded composite product. The thermal analysis of extracted bamboo fiber indicated the thermal degradation behavior of both young and matured bamboo with the same species. The maximum temperature degradation of both young and matured bamboos was 413°C and 404°C. Therefore, matured bamboo was degraded at lower temperature compared to young bamboo, which indicates lower thermal stabilities. The result of this study reported that young bamboo of G. albociliata species is acceptable to be harvested at young ages for reinforcement in natural bonded composite board, according to their thermal stabilities and chemical contents.

Species diversity of primary and secondary forests in Wanglang Nature Reserve

To study the availability of secondary forest for giant pandas (Ailuropoda melanoleuca), we conducted a field survey on the primary and secondary forests in Wanglang Nature Reserve. We compared these two forests based on species diversity and community structure using variance analysis and Mann-Whitney U test. Results showed that, as compared with the primary forest, the secondary forest had more woody plant species, higher tree richness, smaller tree size, smaller regeneration size, and a higher proportion of young bamboo. The secondary forest could provide enough food, species composition, and large-tree environment for giant pandas just as the primary forest does. Importantly, the secondary forest could provide more of the giant pandas’ preferred food than the primary forest, which might be the reason why giant pandas are increasingly using the secondary forest habitat. Thus, we consider the secondary forest as a viable habitat for giant pandas.

Accessing the nursing behaviour of Moso bamboo (Phyllostachys edilus) on carbohydrates dynamics and photosystems

Nursing behaviour, also known as breastfeeding behaviour, is the feeding of juvenile individuals with nutrients or proteins from matures especially in mammals. As a hypothetical phenomenon in bamboo forests, mature bamboos have transferred photoassimilates to young bamboos for recovering and rebuilding their photosystems especially in winter. This process is accompanied by changes in the ability of photosystems and the mass fraction of non-structural carbohydrates (NSCs), structural carbohydrates (SCs), and lignin. We analysed carbohydrates and chlorophyll fluorescence to compare the physiological traits in mature (age 2, 3, 4) and immature (age 1) Moso bamboos (Phyllostachys edilus) during a year using the Portable Chlorophyll Fluorometer (PCF) and the Liquid Chromatographic (LC) method. The results showed that the mass fraction of total soluble carbohydrates (TSCs) and starch in the bottom of bamboo at age 1 was higher than other parts and ages in spring, whereas the mass fraction of TSCs, starch, and sucrose at age 3 was higher than other parts and ages in winter. The Fv/Fm, an indicator to reveal photosystems were functional or not, at age 1 dramatically dropped when the cold current attacked first time in October, and then quickly recovered in November. Our findings indicate that mature bamboos very possibly provide carbohydrates to immature bamboos and help them rebuild their photosystems when a bamboo forest resists cold stress.

POTENSI DAN PEMANFAATAN TANAMAN BAMBU PADA LAHAN MASYARAKAT DI DESA KADING KABUPATEN BARRU

Non-wood forest products (NTFPs) are the result of the forestry sector are still very much needed by the world and can be an opportunity to add value to the economy, especially in the forestry sector. This study aims to determine the potential and utilization of bamboo on community land in Kading Village, Tanete Riaja District, Barru Regency. There are two data collected in this study; they are potential data and bamboo plant utilization data. Potential data collection in this study was carried out in three stages, namely mapping the distribution point of a bamboo clump, collecting data on an area of farmer's garden that has bamboo, and counting the number of bamboo groves on the respondent's bamboo land. Candid interviews with 30 bamboo landowners obtained the data on the use of bamboo plants. The total area of community land covered with bamboo in Kading Village is 7.29 ha with 250 clumps consisting of 2,203 bamboo shoots, 5,345 young bamboo, and 4,532 old bamboos.

Study on growth characteristics of young bamboo and shooting of Chimonobambusa utilis at different altitudes

Study on growth characteristics of young bamboo and shooting of Chimonobambusa utilis at different altitudes

Genetic differentiation of populations of Pseudoregma bambucicola based on mtDNA cytb gene sequences

Pseudoregma bambucicola is an economically significant bamboo pest which is highly reproductive found in Eastern Asia that influences the normal growth of bamboo, induces the formation of sooty molds, and even causes substantial injury or death among young bamboos. This study provides a theoretical and practical basis for the comprehensive prevention and control of Pseudoregma bambucicola. The results showed that 10 geographical populations of Pseudoregma bambucicola showed high genetic diversity (Hd = 0.879 PI + 0.075, =0.08295 + 0.03901) and the average genetic distance was 0.018. In the haplotype phylogenetic tree constructed, the branches of MP tree and BI tree were basically the same, the Sichuan Basin was clustered into one and the populations from other provinces were clustered into a large branch and had a high posterior probability (the posterior probability of MP tree was 99% and the posterior probability of BI tree was 1). The NJ tree showed the differences among the 10 geographies. It was found that the experimental results were consistent with the actual geographical conditions because different geographical Pseudoregma bambusicola experienced natural isolation due to the possible impact of the natural environment, resulting in no genetic exchange between them, thus having a great genetic variability and high genetic diversity.

Feeding habitat characteristics of giant pandas at different scales: A case study in the Wanglang Nature Reserve

To describe the characteristics of feeding sites of giant pandas, we carried out a field survey in the Wanglang Nature Reserve of China. We studied the feeding habitat characteristics of giant pandas at three scales: plot, clump and stem. We found that plots used by giant pandas as feeding sites were characterized by a greater number of bamboo clumps and taller bamboo. The eaten clumps were characterized by a greater abundance of bamboo, more young bamboo stems, and taller average height. The number of bamboo stems eaten had a significantly positive correlation with the total number of bamboo stems in the eaten bamboo clumps. Based on the feeding habitat characteristics at different scales, we considered that giant panda is selective in the use of feeding habitat. They are inclined to use habitat with higher quality bamboo, and feed on more stems in clumps with a greater total number of stems. This study could provide basic data and a reference for understanding the habitat requirements of this species.