Genetic differentiation of populations of Pseudoregma bambucicola based on mtDNA cytb gene sequences

Abstract

Pseudoregma bambucicola is an economically significant bamboo pest which is highly reproductive found in Eastern Asia that influences the normal growth of bamboo, induces the formation of sooty molds, and even causes substantial injury or death among young bamboos. This study provides a theoretical and practical basis for the comprehensive prevention and control of Pseudoregma bambucicola. The results showed that 10 geographical populations of Pseudoregma bambucicola showed high genetic diversity (Hd = 0.879 PI + 0.075, =0.08295 + 0.03901) and the average genetic distance was 0.018. In the haplotype phylogenetic tree constructed, the branches of MP tree and BI tree were basically the same, the Sichuan Basin was clustered into one and the populations from other provinces were clustered into a large branch and had a high posterior probability (the posterior probability of MP tree was 99% and the posterior probability of BI tree was 1). The NJ tree showed the differences among the 10 geographies. It was found that the experimental results were consistent with the actual geographical conditions because different geographical Pseudoregma bambusicola experienced natural isolation due to the possible impact of the natural environment, resulting in no genetic exchange between them, thus having a great genetic variability and high genetic diversity.