Extraction Yield Research Articles

Study of tamarind (Tamarindus indica L.) seed mucilage extraction process using full factorial design of experiments

The processing of tamarind pulp (Tamarindus indica L.) produces residues such as seeds, bark and fibers, which remain underused. Therefore, taking advantage of fruit residues in the development of new products is a technological alternative that can bring economic benefits to producers and positively impact the environment, leading to more diversified products. A byproduct that is less known and explored is the tamarind mucilage, a neutral polysaccharide biopolymer derived from the endosperm of tamarind seeds, in which it has functions of stabilization, emulsification, thickening, coagulation, water retention and film formation. For mucilage extraction, different temperatures (50, 60 and 70 ºC), pH (4, 7 and 10) and mass ratio (0.015; 0.020 and 0.025 g·g−1) were used for a 23 full factorial design. The resulting mucilage was dried in a circulation at 50 °C for 24 h, obtaining an average yield of 0.019 g mucilage·g−1 seed. The extraction yield is dependent on the pH extraction media and the ratio between seeds and solvent. Also, the polysaccharide chemical structure of the mucilage is confirmed by spectroscopic analysis.

Pharmacological Evaluation of Anti-depressant activity of Aegle Marmelos ethanolic leaves extract in mice

Bael (A. marmelos) is an important medicinal herb which has been and extensively used in Ayurveda, Siddha and other medicinal systems. The aim of this study is to evaluate the pharmacological activity of leaves part of the plant Aegle marmelos. Ethanolic extract of Aegle marmelos leaves prepared by successive extraction technique. This research carroed out to find out the Percentage Yield of Extract of Aegle marmelos, determination of LD50 of the EEAML as per OECD guidelines and to investigate the anti-depressant effect of Aegle marmelos leaves extract using tail suspension test (TST) and forced swim test (FST) parameters at dose levels of 200 mg/kg and 400 mg/kg respectively in albino swiss mice. Aegle marmelos leaves extract showed significant antidepressant activity probably due to GABA facilitatory action of phytoconstituents such as flavonoids, tannic acid, marmesinin, phenols, saponin etc. Results suggested that cortisol and corticosterone responded differently to severe stressors with cortisol being a quicker responder than corticosterone. Keywords: Aegle Marmelos, phytoconstituents, Anti-depressant activity

Techno-functionality of jack bean (Canavalia ensiformis) protein concentrate: a comparative study with soy and pea proteins.

With the growing human awareness of the environmental and animal stress caused by the meat industry, the consumption of plant-based products has expanded. Plant proteins have gained market prominence due to their sustainable origin, economic value and health benefits. Well-established plant proteins in the market, such as those of soy and pea, have various applications as ingredients in the food industry. However, given the wide variety of protein sources, it is necessary to conduct studies on the chemical and techno-functional characterization of other raw materials to further diversify their properties. In this context, the present study introduces jack bean protein concentrate (JBPC) as a potential alternative to proteins already established in the market. Techno-functional properties such as surface hydrophobicity, solubility, zeta potential, water- and oil-holding capacity, foam capacity and stability, emulsion stability and gel formation and rheology were analyzed. The protein content obtained from the extraction of the JBPC was 73 g (100 g)-1 on a dry weight basis, with an extraction yield of approximately 10% (w/w). Least gelation concentration for JBPC was 20%. JBPC exhibited a predominantly hydrophobic nature, with good oil retention capacity and emulsion and foam stabilization properties. The structure of JBPC was more linear, stable and rigid, which primarily influenced gel stiffness. Based on the study of techno-functional properties, JBPC proved to be an excellent alternative to soy protein isolate and pea protein concentrate in various applications, with potential for becoming an innovative ingredient in the food industry. © 2025 Society of Chemical Industry.

Physicochemical properties, structure, growth-promoting and antioxidant activities of Glycyrrhiza leaf polysaccharides modified by fermentation

This study aimed to investigate the effects of fermentation on the physicochemical properties and in vitro antioxidant activities of Glycyrrhiza leaf, compare the structures of unfermented (GLP) and fermented (FGLP) polysaccharides, and assess their growth-promoting and antioxidant activities using zebrafish embryos. The results showed that the polysaccharide content, extract yield, and reduced sugar content of Glycyrrhiza leaf increased significantly after 36 h of fermentation. In parallel, in vitro antioxidant activities assays demonstrated that Glycyrrhiza leaf fermented for 36 h exhibited strong DPPH, hydroxyl radical scavenging, and reducing power. Scanning electron microscope and infrared spectroscopy analyses revealed differences in the surface morphology of unfermented and fermented Glycyrrhiza leaf, with both polysaccharides being acidic and containing pyranose rings and α-glycosidic bonds. The molecular weight of FGLP was lower than that of GLP, and the molar ratios of guluronic acid, amino glucose, glucuronic acid, glucose, xylose, and arabinose were increased. In the zebrafish model, Glycyrrhiza leaf polysaccharides promoted the growth and development of zebrafish embryos, with the most pronounced effect at a concentration of 20 µg/mL after fermentation. FGLP exhibited superior protective effects against lipid peroxidation and cell death induced by 2,2′-azobis (2-methylpropionamidine) dihydrochloride. The findings demonstrated that fermentation could enhance the utilization of Glycyrrhiza leaf polysaccharides and their antioxidant capacity. These findings opened avenues for further research into the bioavailability of FGLP in vivo and offered valuable insights into its potential as a natural and innovative antioxidant.HighlightsVariations on physicochemical parameters and antioxidant activity were tracked during fermentation.Fermentation should last for 36 h based on the dynamic changes.The molecular weight of polysaccharides decreased after fermentation.FGLP has growth-promoting and developmental effects on zebrafish.FGLP exhibited superior protective effects against lipid peroxidation and cell death induced by AAPH.Graphical

Effect of Planting Systems on the Physicochemical Properties and Bioactivities of Strawberry Polysaccharides

Suitable planting systems are critical for the physicochemical and bioactivities of strawberry (Fragaria × ananassa Duch.) polysaccharides (SPs). In this study, SPs were prepared through hot water extraction, and the differences in physicochemical characteristics and bioactivities between SPs derived from elevated matrix soilless planting strawberries (EP-SP) and those from and conventional soil planting strawberries (GP-SP) were investigated. A higher extraction yield was observed for EP-SP (5.88%) than for GP-SP (4.67%), and slightly higher values were measured for the average molecular weight (632.10 kDa vs. 611.88 kDa) and total sugar content (39.38% vs. 34.92%) in EP-SP. In contrast, a higher protein content (2.12% vs. 1.65%) and a more ordered molecular arrangement were exhibited by GP-SP. Monosaccharide composition analysis revealed that EP-SP contained higher levels of rhamnose (12.33%) and glucose (49.29%), whereas GP-SP was richer in galactose (11.06%) and galacturonic acid (19.12%). Thermal analysis indicated only minor differences in decomposition temperatures (approximately 225–226 °C) and thermal stability between the samples. However, GP-SP showed a higher enthalpy change (ΔHg = 18.74 J/g) compared to EP-SP (13.93 J/g). Biological activity assays revealed that GP-SP generally exerted stronger non-enzymatic glycation inhibition at both early and final stages (IC50: 7.47 mg/mL vs. 7.82 mg/mL and 11.18 mg/mL vs. 11.87 mg/mL, respectively), whereas EP-SP was more effective against intermediate α-dicarbonyl compounds (maximum inhibition of 75.32%). Additionally, GP-SP exerted superior α-glucosidase inhibition (IC50 = 2.4583 mg/mL), in line with kinetic and fluorescence quenching analyses showing a higher enzyme–substrate complex binding affinity (Kis = 1.6682 mg/mL; Ka = 5.1352 × 105 M−1). Rheological measurements demonstrated that EP-SP solutions exhibited a pronounced increase in apparent viscosity at higher concentrations (reaching 3477.30 mPa·s at 0.1 s−1 and 70 mg/mL) and a stronger shear-thinning behavior, while GP-SP showed a comparatively lower viscosity and lower network order. These findings suggest that different planting systems significantly affect both the molecular structures and functionalities of SPs, with GP-SP demonstrating enhanced hypoglycemic and anti-glycation properties. It is therefore recommended that suitable planting systems be selected to optimize the functionality of plant-derived polysaccharides for potential applications in the food and pharmaceutical industries.

Direct Hot Solid–Liquid Extraction (DH-SLE): A High-Yield Greener Technique for Lipid Recovery from Coffee Beans

Soxhlet extraction is a method recommended by the Association of Official Analytical Chemists (AOAC) to determine the lipid content in plant samples. Generally, n-hexane (toxicity grade 5) is used as the solvent (≈300 mL; ≈30 g sample) at boiling temperatures (69 °C) for long times (≤16 h) under a chilled water reflux (≈90 L/h), proportionally aggravated by the number of repetitions and samples determined. In this sense, the technique is neither safe nor sustainable for the analyst or the environment. This article presents the development of an alternative and more sustainable procedure for determining the lipid content in raw Arabica coffee beans. A 33 full factorial design was used to perform direct hot solid–liquid extractions in 4 mL vials, varying the ground grains and solvent ratios, temperatures, and times. An optimal condition resulted in an extractive yield statistically equivalent to Soxhlet, without variation in the composition of the oil fatty acids determined by GC-MS after hole oil transesterification. This procedure was presented as a sustainable alternative to Soxhlet extraction because it does not require water for cooling and needs a smaller volume of solvent (2 mL) and sample mass (0.2 g); it also has a smaller generated residue, as well as requiring a shorter time (1.5 h) and less energy expenditure for extraction.

Cream Formulation Of Methanol Extract Of Tapak Kuda Leaves (Ipomoea Pes Caprae (L.) R. Br.) As Anti-Inflammatory in Male White Mice (Mus Musculus)

The study entitled “Cream Formulation of Methanol Extract of Tapak Kuda (Ipomoea pes-caprae (L.) R. Br.) Leaves) As an anti-inflammatory in male white mice (Mus musculus)” using laboratory experimental methods with posttest control group design, data analysis using SPSS 25.0 with One Way ANOVA test and Duncan test. The percentage of extract yield obtained was 3.12%, phytochemical screening test showed the presence of secondary metabolite compounds alkaloids, terpenoids, flavonoids, saponins, tannins and steroids. Cream preparation of methanol extract of Tapak Kuda leaves at concentrations of 2.5%, 5% and 10% showed anti-inflammatory activity and the maximum dose to reduce edema diameter was 10% concentration with a percentage inhibition of 87.97%. Data analysis with One Way ANOVA test with a significance value ≤ 0.05 which indicates the presence of anti-inflammatory activity in Ipomoea pes-caprae tread leaf methanol extract cream.

Comparative Study on the Extraction of Crude Fucoidan from Brown Seaweed using Conventional, Microwave and Ultrasound-Assisted Methods

Brown seaweeds are rich in fucoidan, a sulfated polysaccharide with antimicrobial, immunomodulatory, antioxidant, and anticancer properties. The brown algae Sargassum sp. has not been thoroughly investigated for fucoidan extraction using various techniques and evaluations of their effects on extraction yield and its structural properties. The purpose of this study was to compare the structural characteristics and extraction yield of fucoidan from Sargassum sp. using conventional, microwave, and ultrasonic-assisted extraction methods. The results showed that a slightly higher yield was obtained by using the ultrasonic-assisted extraction method (2.772%) followed by the microwave-assisted extraction method (2.494%) and conventional extraction method (2.399%). However, the IC50 values for antioxidants were found to be lower (less value is preferable) for crude fucoidan obtained by microwave-assisted extraction method (175 ?g/ml) than for conventional (195 ?g/ml) and ultrasound-assisted extraction methods (230 ?g/ml). The crude fucoidan obtained from the three different extraction methods showed moderate antioxidant strength Contribution to Sustainable Development Goals (SDGs)SDG 3: Good Health and Well-beingSDG 9: Industry, Innovation, and InfrastructureSDG 12: Responsible Consumption and ProductionDG 14: Life Below Water

An Overview on Isolation & Evaluation of Flaxseed Mucilage and its use as a Pharmaceutical Excipients

Flaxseed contains significant concentration of mucilage or gum (a type of hydrocolloid). Flaxseed mucilage (FM) predominantly occurs in the outermost layer of the seed’s hull and is known to possess numerous health benefits such as delayed gastric emptying, reduced serum cholesterol, and improved glycemic control. FM is typically composed of an arabinoxylan (neutral in nature) and a pectic-like material (acidic in nature). Similar to gum arabic, FM exhibits good water-binding capacity and rheological properties (similar functionality); therefore, FM can be used as its replacement in foods. In this review, an overview of methods used for FM extraction and factors influencing the extraction yield were discussed initially. Thereafter, food applications of FM as gelling agent/gel-strengthening agent, structure-forming agent, stabilizing agent, fat replacer, anti-retrogradation agent, prebiotic, encapsulating agent, edible coatings and films/food packaging material, and emulsifier/emulsion stabilizer were included. At the end, some limitations to its wide application and potential solutions were added.

Formulation of sustainable, biodegradable chitosan films enriched with Origanum majorana extract as an eco-friendly antimicrobial food packaging for possible food preservation.

In this work, chitosan (Cs) was blended with different concentrations of Origanum majorana extract (OmE) for the formation of food packaging films. Based on the utilized volumes of OmE (2.5, 5, and 7.5 mL) that were mixed with 27.5, 25, and 22.5 mL of Cs, there different film samples (2.5 % OmE loaded Cs film, 5 % OmE loaded Cs film and 7.5 % OmE loaded Cs film) were obtained and compared with the film of pure Cs. The extraction yields of OmE were found to be 13 g and 10.4 g % when using ethyl acetate and methanol, respectively, with total phenolic content measured at 889.30 μg GAE/g for the ethyl acetate extract and 810.21 μg GAE/g for the methanol extract, indicating a substantial amount of bioactive compounds available for formulation. Antimicrobial activity was assessed against various foodborne pathogens, with the 7.5 % OmE-loaded Cs film demonstrating the highest efficacy, achieving inhibition zones of 27 mm against E. coli and 25 mm against S. aureus. This research underscores the potential of Cs-based films enriched with O. majorana extract as a viable solution for active food packaging, addressing environmental concerns and food safety.

Extraction and characterization of Eucommia ulmoides gum from Eucommia leaves by fermentation with Coprinellus disseminatus.

To determine the optimum conditions for extracting Eucommia ulmoides gum (EUG) from Eucommia ulmoides leaves during fermentation by Coprinellus disseminatus. At the same time, the EUG characteristics were characterized. The ability of C. disseminatus to secrete degrading enzymes, including enzyme activity and lignocellulose content, was observed by culturing C. disseminatus on a medium with different formulations. Based on the optimal formula, the fermentation conditions were determined by an orthogonal test and EUG was extracted. After that, the characteristic parameters of EUG were determined by FT-IR, 1H-NMR, SEM, POM, DSC, XRD and GPC. The results showed that the lignin and hemicellulose contents in Eucommia leaves decreased significantly with the increase of fermentation days, and C. disseminatus mediated fermentation had an obvious damage effect on the tissue of Eucommia leaves. At the same time, At the same time, xylan can be used as an initiator to induce the expression of degradation-related enzymes. The extraction yield of EUG from Eucommia ulmoides leaves after fermentation was 3.06%, which was significantly improved. The optimum fermentation conditions were 4% inoculum, 28°C fermentation temperature, and pH=5. The results of infrared FT-IR and 1H-NMR showed that the extract had typical characteristics of EUG, and the surface and quenching part of the EUG were smooth, as observed by SEM. In addition, DSC and XRD analysis show that there are two crystal types, α and β, in the crystallization process of molten EUG. The main crystallization type of EUG extracted from fermented leaves was β type. The ability of C. disseminatus to produce degrading enzymes can effectively improve the extraction rate of EUG under optimal fermentation process. The method can maintain the integrity of gutta-percha better. EUG has the characteristics of good crystallinity, many β crystal types and high molecular weight.

Effect of extraction conditions on antioxidant activities of Xanthium strumarium L. sprouts extracts

This research aims to obtain the Xanthium strumarium sprouts extract with the most potent antioxidant activities. The single-factor experiments were used to extract. Factors of extraction, including temperatures (40, 50, 60, and 70°C), time (6, 8, 10, 20, and 30 minutes), and % ethanol in solvent (40, 50, 60, and 70 % EtOH), were applied in the ultrasonic assist extraction of 40°C oven-dried Xanthium strumarium sprouts 1 gram in 25 mL of solvent. Extraction yield (% yield) and % inhibition of active radical DPPH and ABTS were determined, and the correlation to varied factors was analyzed using a statistical program (SPSS). The results showed that, at low temperatures, it would give a low % yield but not affect antioxidant activities. The longer extraction time did not provide a different % yield than the shorter one. The extraction yield is a little better when 50% ethanol is used in water as a solvent and does not affect antioxidant activities. As they observe, we can conclude that the antioxidant activities of extracts do not depend on temperature, time, or % EtOH in solvents. The % inhibition of ABTS radicals is higher than that of DPPH radicals in all experiments, and it can be relied on that the active components in this extract are more polar and favorable to solute in water than EtOH.

Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study.

Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol's environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28% extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48-174.64kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.

Genomic prediction for yield and malting traits in barley using metabolomic and near-infrared spectra

Key messageGenetic variation for malting quality as well as metabolomic and near-infrared features was identified. However, metabolomic and near-infrared features as additional omics-information did not improve accuracy of predicted breeding values.Significant attention has recently been given to the potential benefits of metabolomics and near-infrared spectroscopy technologies for enhancing genetic evaluation in breeding programs. In this article, we used a commercial barley breeding population phenotyped for grain yield, grain protein content, and five malting quality traits: extract yield, wort viscosity, wort color, filtering speed, and β-glucan, and aimed to: (i) investigate genetic variation and heritability of metabolomic intensities and near-infrared wavelengths originating from leaf tissue and malted grain, respectively; (ii) investigate variance components and heritabilities for genomic models including metabolomics (GOBLUP-MI) or near-infrared wavelengths (GOBLUP-NIR); and (iii) evaluate the developed models for prediction of breeding values for traits of interest. In total, 639 barley lines were genotyped using an iSelect9K-Illumina barley chip and recorded with 30,468 metabolomic intensities and 141 near-infrared wavelengths. First, we found that a significant proportion of metabolomic intensities and near-infrared wavelengths had medium to high additive genetic variances and heritabilities. Second, we observed that both GOBLUP-MI and GOBLUP-NIR, increased the proportion of estimated genetic variance for grain yield, protein, malt extract, and β-glucan compared to a genomic model (GBLUP). Finally, we assessed these models to predict accurate breeding values in fivefold and leave-one-breeding-cycle-out cross-validations, and we generally observed a similar accuracy between GBLUP and GOBLUP-MI, and a worse accuracy for GOBLUP-NIR. Despite this trend, GOBLUP-MI and GOBLUP-NIR enhanced predictive ability compared to GBLUP by 4.6 and 2.4% for grain protein in leave-one-breeding-cycle-out and grain yield in fivefold cross-validations, respectively, but differences were not significant (P-value > 0.01).

Application of indole-3-butyric acid (IBA) enhances agronomic, physiological and antioxidant traits of Salvia fruticosa under saline conditions: a practical approach.

Salinity stress is a significant challenge in agriculture, particularly in regions where soil salinity is increasing due to factors such as irrigation practices and climate change. This stress adversely affects plant growth, development, and yield, posing a threat to the cultivation of economically important plants like Salvia fruticosa. This study aims to evaluate the effectiveness by proactively applying indole-3-butyric acid (IBA) to Salvia fruticosa cuttings as a practical and efficient method for mitigating the adverse effects of salinity stress. The factors were arranged as three different IBA doses (0, 1, and 2 g/L) and four different salinity concentrations (0, 6, 12, and 18 dS/m) in controlled greenhouse conditions. Plant height (PH), flower spike length (FSL), fresh shoot length (FRL), root length (RL), fresh root weight (FRW), fresh shoot weight (FSW), dried root weight (DRW), dried shoot weight (DSW), root/shoot index, drog (g/plant), relative water content (RWC), relative membrane permeability (RMP), chlorophyll content (SPAD), extraction yield (%), DPPH (2,2-Diphenyl-1-picrylhydrazyl), phenol content, flavonoid content, and ABTS (2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) values were measured. The results show that as salinity doses increased, all parameters showed a decline. However, with a one-time IBA application to the plant cuttings before the rooting stage, particularly at a concentration of 2 g/L, was effective for mitigating the negative effects of salinity stress. Across all measured parameters, IBA significantly reduced the adverse impacts of salinity on Salvia fruticosa.

Enhancement of health beneficial bioactivities of bitter melon (Momordica charantia L.) by puffing.

Effects of puffing and extraction method on physical and biological efficacy of bitter melon was investigated. Puffing increased the Maillard reaction products, extraction yield, total phenolic and total flavonoid contents. Antioxidant activity was the highest at 980kPa, but there was no significant difference between two extraction methods. However, methanol extraction showed higher anti-inflammatory, anti-obesity and anti-diabetic activities. Charantin was detected only in methanol extraction and increased with increasing puffing pressure. In addition to the remarkable increase in antioxidant activity, puffing could increase the charantin contents of BM. Methanol extract showed better biological efficacy excluding antioxidant activity than those of hot water extraction. Overall, puffing can be used as a method for increasing the functionality of BM, and it may be effective to consume puffed BM by itself such as the chip or powder forms without extraction for liberation and absorption of bioactive substances for the human body.

Switchable Deep Eutectic Solvents for the Green Extraction of Tea Saponins From Camellia Oleifera Seed Meal.

Tea saponin has garnered tremendous interest for its potential use in surfactant and drug synthesis. This research was designed to develop a technique based on pH-responsive switchable deep eutectic solvents (SDESs) for extracting tea saponins from Camellia oleifera seed meal. SDES synthesized from hexanoic acid and triethanolamine (1:1 molar ratio) offered the optimum extractive performance and the optimal conditions were obtained through single-factor experiments: 30 wt% water content in SDES, solid-liquid ratio of 1:30 g/mL, 60°C extraction temperature, 30min extraction time, and acid volume of 1500µL. The extraction yield was 162.14 mg/g after optimization, which increased by 15.65% and 9.47% compared to water and ethanol extraction methods, respectively. After pH adjustment, 98.95% of the tea saponins were recovered in the bottom phase. After precipitation and freeze-drying procedures, the purity of the tea saponin solid was 62.9%.

Innovative CO2-NBs-assisted ultrasonication for the phytochemical extraction of peanut (Arachis hypoga) shells: Synthesis and characterization of CO2-nanobubbles.

This study was designed to obtain the maximum extraction yield of peanut shell (PS) polyphenols using a novel carbon dioxide nanobubbles (CO2-NBs) assisted ultrasonic extraction method. CO2-NBs were generated in distilled water with a self-developed high-pressure nano-jet homogenization method and characterized by size, zeta potential and transmission electron microscopy (TEM). The obtained nanobubble's mean size and zeta potential were 229.96±17.44nm and -15.9±1.27mV, respectively. Later, these CO2-NBs, combined with ultrasonic method, were used for the extraction of polyphenols, achieving the highest polyphenol content (3619.21±113.07 µg GAE/mL) as compared to ultrasonic extraction (2914.69±145.45µg GAE/mL) and conventional extraction (2340.11±80.02µg GAE/mL). Response surface methodology (RSM) provided optimization parameters, including ultrasonic power of 358.76W, surfactant concentration of 4.54%, and extraction time of 41.41min. HPLC analysis identified distinct peaks corresponding to polyphenolic compounds such as gallo-catechin, catechin gallate, resveratrol, and luteolin, confirming their presence and concentrations in the peanut shell extract (PSE). Scanning electron microscopy (SEM) revealed significant structural disruption and increased porosity in peanut shell powder, supporting the enhanced extraction of polyphenols through CO2-NBs-assisted ultrasonic extraction process. This research establishes theoretical and practical foundation for generation of CO2-NBs and CO2-NBs ultrasonic extraction technology to efficiently extract polyphenols from waste PS, thereby enhancing the extraction efficiency of valuable compounds for use in functional food products and promoting sustainable practices in food industry.

Extraction Mechanism of Hesperidin from Citrus aurantium L. Using a Novel Deep Eutectic Solvent: Experimental and Theoretical Investigation

In order to extract hesperidin from Citrus aurantium L. through green and efficient ways, a novel method based on deep eutectic solvent (DES) assisted extraction has been developed. Compared to known alkali extraction and acid precipitation or organic solvent (ethanol, methanol) extraction, this new DES system of choline chloride/diethanolamine (DES-14) method exhibited excellent extraction efficiency for hesperidin. Under optimal conditions, the extraction yield of hesperidin reached 6.26 ± 0.05%. Further studies with infrared spectroscopy, 1 H nuclear magnetic resonance, and density functional theory showed the DES-14 was formed mainly through the interaction of H-bond and van der Waals forces. In addition, scanning electron microscopy and density functional theory revealed the strong dissolving capacity, and the H-bond formation between DES-14 and hesperidin, as well as van der Waals forces all contributed to efficient extraction of hesperidin. The results provide a new strategy for efficient extraction and utilization of hesperidin in Citrus.

Study on Antibacterial Activities of Croton macrostachyus and Pycnostachys abyssinica Leaf Extracts Against Some Human Pathogens

The present study was aimed to verify the medicinal value of Croton macrostachyus and Pycnostachys abyssinica traditionally used to treat human and animal ailments in Ethiopia. Fresh leaves of these species were collected, dried under shade, and ground into fine powder. The extraction was carried out by the maceration method using methanol as a solvent. The compounds in the crude extract were further profiled by thin layer chromatography (TLC). The antibacterial activity of the compounds was evaluated using the agar well diffusion method. C. macrostachyus had a higher percentage extract yield (22.5%) than P. abyssinica (13.7%). The phytochemical screening showed more abundant phenolic compounds and tannins in the extract of C. macrostachyus and alkaloid, flavonoid, phenolic compounds, and tannins in the extract of P. abyssinica. Overall, C. macrostachyus produced twofold higher growth inhibition zone (24.0 ± 0.5–43.0 ± 1.0 mm) against the tested pathogens than P. abyssinica (7.0 ± 0.5–24.0 ± 0.3 mm). Among the bacteria, Klebsiella pneumoniae was found to be the most sensitive to compounds of C. macrostachyus. The lowest minimum inhibitory concentration (MIC) values (25 mg/mL) were obtained from compounds 1 and 3 of C. macrostachyus leaf extract against Escherichia coli and K. pneumoniae and compounds 2 and 3 of the same against S. aureus. Similarly, compound 1 of P. abyssinica leaf extract produced the lowest MIC (25 mg/mL) against K. pneumoniae and Staphylococcus epidermidis and compound 3 of the same species against E. coli and K. pneumoniae. All the profiled compounds of the two species had both bactericidal and bacteriostatic properties against the tested pathogens. The results of the present study revealed that the compounds of C. macrostachyus had strong antibacterial activity against all the tested pathogens, indicating the high potential of the compounds. However, further characterization and identification of the specific compounds for the development of biocide formulations are suggested.