Yellow-legged Hornet Vespa Velutina Research Articles

Invasion genetics of the yellow-legged hornet Vespa velutina in the Westernmost Mediterranean archipelago

AbstractThe yellow-legged hornet (Vespa velutina) is a social Hymenoptera native from Asia and an invasive species in Europe, where it was first detected in France in 2004. Since then, the species has spread across the continent invading mainland Spain and Mallorca island (Balearic archipelago, Western Mediterranean) in 2010 and 2015, respectively. Yellow-legged hornets cause severe damage to ecosystem by predating over a wide variety of pollinators including honeybees. Such a threat situation requires the development of effective management and prevention plans, which can greatly benefit from knowing both the origin and the genetic structure of the invading populations. Here we conduct a genetic study to shed light on both the origin and the phylogenetic relationships of V. velutina populations from Mallorca and mainland Spain using nuclear (STRs) and mitochondrial (cytochrome oxidase c subunit 1) gene markers. Our results show that Mallorca populations originated from invasive European specimens. Moreover, FST values, DAPC and genetic structure analysis suggest two independent incursions in the island with bottleneck and founder effect signatures. Finally, we contribute additional genetic evidence of the polyandrous behavior of this invasive species based on the inference of a mean number of mattings per nest of 3.94 (range 2–6.5). This study supports the human-mediated pathways of this species and highlights the importance of implementing effective biosecurity measures to prevent the spread of invasive alien species in island habitats.

All That Glitters Is Not Gold: The Other Insects That Fall into the Asian Yellow-Legged Hornet Vespa velutina 'Specific' Traps.

Simple SummaryThe recent spreading of the invasive Asian hornet (Vespa velutina) to the Iberian Peninsula has led to the application of management measures to control and mitigate its impact on receiving environments. Among the most used control methods are capture traps, which use a sugary attractant to catch the invasive wasps. However, although the used V. velutina traps are presumably specific, they do not only attract V. velutina specimens, but also a large number of non-target species that are also captured. In the present work, the species of insects that unintentionally fall into the capture traps of V. velutina have been specifically identified, as well as their implications for ecosystem and for human activities. A total of 74 non-target taxa of insects were caught by the V. velutina trapping in northern Spain. Most of them were flies, mosquitoes, wasps and moths, being all highly important groups from the biological, ecological and economical points of view. Surprisingly, the most abundant trapped species was the invasive fly, Drosophila suzukii that represented the 36.07% of the total catches. Furthermore, we reported the first record of ectoparasitic mites of the genus Varroa on V. velutina, constituting a newly recorded symbiotic association.The introduction of invasive species is considered one of the major threats to the biodiversity conservation worldwide. In recent years, an Asian invasive species of wasp has set off alarms in Europe and elsewhere in the world, Vespa velutina. The Asian wasp was accidentally introduced in France around 2004 and shortly thereafter it was able to colonise practically all of Europe, including the Iberian Peninsula. The ecological and economic implications of V. velutina invasion and its high colonisation ability have triggered widespread trapping campaigns, usually supported by beekeepers and local governments, with the aim of diminishing its population and its negative impacts. Among the most used control methods are the capture traps, which use a sugary attractant to catch the invasive wasps. However, the species-specific selectivity and efficiency of these traps has been little studied. In this paper, we have analysed the specific identity of the unintentionally trapped insect species from northern Spain (covering one-year period), as well as we have assessed the provided ecosystem services by them. A total of 74 non-target taxa of insects were caught by the V. velutina studied traps, most of them correspond to the orders Diptera, Hymenoptera and Lepidoptera, the dipterans being the most abundant group. Surprisingly, the most abundant trapped species was the invasive fly, Drosophila suzukii that represented the 36.07% of the total catches. Furthermore, we reported the first record of ectoparasitic mites of the genus Varroa on V. velutina, constituting a newly recorded symbiotic association. Hopefully, the provided information helps to develop new protocols and management tools to control this invasive species in the Iberian Peninsula and other temperate areas of western Europe and the Mediterranean basin.

Rearing techniques for hornets with emphasis on Vespa velutina (Hymenoptera: Vespidae): A review

• Vespa velutina is an invasive alien species of Vespa hornet in Korea, Japan and European countries. • Biological, ecological, medicinal, and culinary motivations justify hornet rearing. • Hornet, bumble bee, yellowjacket, and paper wasp rearing techniques are similar. • Year-round hornet rearing has been accomplished in China but is yet undeveloped. • Current methods of vespa rearing were synthesized, and key challenges are identified and discussed. Of the 34 vespid species recorded worldwide as invasive aliens, particular attention is currently being given to the yellow-legged hornet Vespa velutina that has invaded Europe, Japan, and Korea. The hornet is a voracious predator of bees and a serious threat to bee colonies and bee pollination. Control measures are needed, but their development has been challenging as biological and ecological studies are limited by the short field season and the cryptic nesting behavior of these hornets. A Vespa rearing process can generate the large numbers of workers, gynes, and males that are essential for studying chemical ecology and life history and for experimental testing of various hypotheses. We present a synthesis of suitable methods and techniques for year-round Vespa hornet rearing. Particular reference is given to Chinese know-how and experience with Vespa rearing for medicinal and culinary motivations. We also draw on observations with reared insects that have a similar life history as Vespa hornets, namely Bombus bumblebees and Vespula yellowjackets. Key challenges to optimizing Vespa hornet rearing are identified and discussed.

Nest structures display specific hydrocarbon profiles: insights into the chemical ecology of the invasive yellow-legged hornet Vespa velutina nigrithorax

In insects, chemical communication is the most common form of communication, and cuticular hydrocarbons (CHCs) are employed in recognition processes. In social insects, CHCs also help define colony identity and thus contribute to social cohesion among nestmates. Individuals can deposit their chemical signatures on nest surfaces. This information serves as a reference for newly emerged individuals and allows them to obtain the odor specific to their colony. This study examined nest chemical profiles in an inbred invasive species: the yellow-legged hornet, Vespa velutina nigrithorax. We demonstrated that nest structures (i.e., envelopes, combs, and pillars) had specific hydrocarbon profiles, which were colony specific. There were similarities between the chemical profiles of the nests and the CHC signatures of hornets. The loss of genetic diversity previously documented in the yellow-legged hornet population in France does not appear to have constrained nest chemical diversity.

Effectiveness and Selectiveness of Traps and Baits for Catching the Invasive Hornet Vespa velutina.

Simple SummaryThe introduction of invasive species is one of the major causes of biodiversity loss and, in many cases, entails considerable economic consequences. Due to their biological traits, social Vespidae are known for their potential in establishing viable populations and become invasive in many countries of the world. A good example is represented by the invasion success of the Asian yellow-legged hornet Vespa velutina, a species that has been introduced and established in Europe and other countries of Asia. By preying on honey bees and native insects, this species is a threat for the biodiversity, the pollination ecosystem service and the economy. Traps for wasps and hornets are widely used for monitoring the presence of V. velutina or, at a rather higher density, as a complementary method for limiting its impacts. Here we compared the performance of two typologies of traps and baits widely used for trapping this invasive species and, at the same time, evaluated the consequences of this activity on native insects. Findings highlighted that the performance of the trap/bite combinations changed in relation to the season. However, the proportion of non-target insects in the traps stress the necessity of developing alternative monitoring and control techniques.Vespa velutina is an invasive hornet that is colonising several countries worldwide, with detrimental effects on multiple components but primarily affecting honey bees and native insect species. Traps for wasps and hornets are commonly used for trapping V. velutina, both for monitoring and control purposes. In this study, we compared the performances of two typologies of traps and baits widely used for trapping this invasive hornet, by evaluating their effectiveness and selectiveness in trapping V. velutina in two sites during two different periods of the year, spring and autumn. The performance of the traps changed in relation to (i) the trap’s model, (ii) the bait’s typology and (iii) the period of the year. In spring, traps with common beer as bait were more effective and more selective independently of trap’s model than the commercial bait that has been tested. On the contrary, in autumn, just one combination of trap and attractant (the commercial trap and bait) achieved higher effectiveness and selectiveness. Despite the underlined variations among traps and baits, overall catches of V. velutina were scanty compared to bycatches of non-target insects, since best performing traps either in term of effectiveness and selectiveness caught 3.65% of the target species in spring and 1.35% in autumn upon the total trapped insects. This highlights the urgent necessity of developing more selective trapping methods for monitoring and particularly for controlling purposes.

Viruses in the Invasive Hornet Vespa velutina.

The Asian yellow-legged hornet Vespa velutina nigrithorax, a major predator of honeybees, is spreading in Europe in part due to a lack of efficient control methods. In this study, as a first step to identify biological control agents, we characterized viral RNA sequences present in asymptomatic or symptomatic hornets. Among 19 detected viruses, the honey bee virus Deformed wing virus-B was predominant in all the samples, particularly in muscles from the symptomatic hornet, suggesting a putative cause of the deformed wing symptom. Interestingly, two new viruses closely related to Acyrthosiphon pisum virus and Himetobi P virus and viruses typically associated with honey bees, Acute bee paralysis virus and Black queen cell virus, were detected in the brain and muscles, and may correspond to the circulation and possible replication forms of these viruses in the hornet. Aphid lethal paralysis virus, Bee Macula-like virus, and Moku virus, which are known to infect honey bees, were also identified in the gut virus metagenome of hornets. Therefore, our study underlined the urgent need to study the host range of these newly discovered viruses in hornets to determine whether they represent a new threat for honey bees or a hope for the biocontrol of V. velutina.

Reproductive interference by alien hornet Vespa velutina threatens the native populations of Vespa simillima in Japan.

The yellow-legged hornet Vespa velutina has become one of the major alien species in European and East Asian countries. As in its homeland, the invading V. velutina is reported as the major predator of honeybees and is becoming a threat to beekeeping in Europe. However, it remains unknown how V. velutina might affect native hornets when it invades Asia, where a large number of Vespa species are distributed. Thus, by analyzing the mitochondrial cytochrome c oxidase subunit I gene, we investigated whether interspecific mating occurs between V. velutina and Japanese native Vespa spp. Our results showed that the alien V. velutina causes reproductive interference in Japanese native hornet Vespa simillima. Forty-three percent of native V. simillima queens had the sperm of V. velutina males, and among the all V. simillima queens analyzed, 28% only had V. velutina sperm. We did not find evidence of V. velutina queens having the sperm of V. simillima males. These findings suggest that reproductive interference by V. velutina males poses a threat to the native V. simillima populations. A decline of V. simillima may also negatively affect other insects that interact with V. simillima.

Abundance of diet-derived polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in the bodies and nests of the yellow-legged hornet Vespa velutina nigrithorax and risks to human health in South Korea

Detecting polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in an organism's environment is ecologically significant. In particular, the detection of PCDD/Fs in organisms consumed as food suggests the possibility of human health risks. In South Korea, there is a unique custom in which social wasps, such as hornets, have been used as ingredients in traditional liquor. Thus, we investigated the concentrations and patterns of PCDD/Fs for the most common social wasps, the yellow-legged hornet (Vespa velutina nigrithorax), and estimated human health risks based on potential dietary exposure in traditional liquor made from the polluted wasps and their nests. The mean concentration of ∑17 PCDD/Fs in the wasps and nests of V. v. nigrithorax was 33.59 pg/g (median 25.31 pg/g), corresponding to 0.65 pg World Health Organization – Toxic equivalency factor (WHO-TEQ/g) (median 0.49 pg WHO-TEQ/g). The levels of ∑17 PCDD/Fs from the nests of V. v. nigrithorax were higher than those in the adults and larvae. As a result of this risk assessment, the daily intake of traditional liquor made with these wasps and their nests was found to be below safety levels; however, there could still be a possibility for health risks due to the detection of PCDD/Fs from organism. Therefore, continuous monitoring of environmental contaminants, including PCDD/Fs in wasps and nests, is required in order to evaluate human health risks associated with the consumption of traditional liquor made with these biological substances. In addition, the results of this study can be used to calculate dietary exposure to PCDD/Fs and the extent of risk to food in which the organism is consumed.

Female body size, weight and fat storage rather than nestmateship determine male attraction in the invasive yellow-legged hornet Vespa velutina nigrithorax

In the early stage of the invasion process, alien species may face costs linked to pioneer effect due to genetic bottleneck, drift and the consequential inbreeding depression. Thus, introduced species that show an immediate exponential growth in their invasive population should have some mechanisms to reduce such costs minimising the chance of inbreeding or allowing them to cope with them. The yellow-legged hornet Vespa velutina nigrithorax has been spotted in France in 2004; since then, the species has been invading Europe with a relentless pace. In their native range, males and reproductive females of a Chinese non-invasive sub-species of V. velutina seem to leave their nests to search for unrelated partners. However, previous studies showed a low genetic diversity and a high rate of diploid males in colonies of the invasive population, suggesting that mating could occur inside nests, where males should be able to discriminate between reproductive gynes and sterile workers. Here, we used laboratory behavioural assays to investigate the mating preferences of yellow-legged hornet males from the recent invasive population in Italy. We assessed the importance of nestmateship and female morpho-physiological traits, likely indicators of caste, in determining male attraction towards potential partners. Our results demonstrate that males are more attracted to bigger females with more abundant fat storage, good indicators of female reproductive caste in wasps, regardless of nestmateship. Our findings represent a first step in understanding the reproductive biology of V. velutina nigrithorax in its invasive range, providing a framework for future research in the field to prevent or reduce the species expansion.

Spread of the invasive yellow-legged hornet Vespa velutina (Hymenoptera: Vespidae) in Italy

The yellow-legged hornet Vespa velutina Lepeletier 1836, accidentally introduced into France in 2004, is rapidly colonizing other European countries. In Italy the species is spreading throughout the northwest part of the country. Setting up management plans for controlling invasive alien species requires an understanding of the spread modalities and distribution range of the species, information currently not available for the yellow-legged hornet in Italy. The aims of this work are to reconstruct the spread of the yellow-legged hornet from its first arrival in the country, evaluating its distribution range and spread modalities. The area occupied by the species increased from 205 km2 in 2013 to 930 km2 in 2015. In 2015 the frontline of the species was at 55 km along the coast from the French border, with a linear spread of 18.3 ± 3.3 km/year. A human-mediated dispersion could be recognized in different occasions. A cluster analysis of the range allowed the identification of 17 core areas used by the species, with a mean nest density of 2.9–3.5 nests/km2. These details are fundamental to improve control plans and to establish an early warning and rapid response system for the yellow-legged hornet in Italy and, therefore, setup an effective management plan for the species.

Production of Early Diploid Males by European Colonies of the Invasive Hornet Vespa velutina nigrithorax.

The invasive yellow-legged hornet Vespa velutina nigrithorax was accidentally introduced in Europe in the early 2000s. As is the case in colonies of other wasp and hornet species, V. velutina colonies are known to produce sexuals (males and new queens) at the end of the summer. We show that early-stage colonies in French populations frequently produce males well before the usual reproductive period. The vast majority of the males produced are diploid, which is consistent with the loss of genetic diversity previously reported in introduced populations in France. Since males do not participate in colony activities, the production of early diploid males at the expense of workers is expected to hamper colony growth and, ultimately, decrease the expansion of the species in its invasive range in Europe.

Corrigendum

EPPO BulletinVolume 45, Issue 2 p. 328-328 CorrigendumFree Access Corrigendum This article corrects the following: Spread of the yellow-legged hornet Vespa velutina nigrithorax du Buysson (Hymenoptera: Vespidae) across Northern Spain A. Goldarazena, I. P. Heredia, P. Romon, J. C. Iturrondobeitia, M. Gonzalez, S. Lopez, Volume 45Issue 1EPPO Bulletin pages: 133-138 First Published online: March 26, 2015 First published: 27 July 2015 https://doi.org/10.1111/epp.12219AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Bulletin OEPP/EPPO Bulletin 45 (2015), 133–138. Since the publication of the article Goldarazena et al. (2015) Spread of the yellow-legged hornet Vespa velutina nigrithorax du Buysson (Hymenoptera: Vespidae) across Northern Spain it has been brought to our attention that the affiliation provided for Mr Sergio López was incorrect. The correct affiliation for Mr Sergio López is Departamento de Zoología y Dinámica Celular, Universidad del Paıs Vasco, Sarriena s/n Leioa, Bilbao, Spain. Reference OEPP/EPPO (2015) Spread of the yellow-legged hornet Vespa velutina nigrithorax du Buysson (Hymenoptera: Vespidae) across Northern Spain. Bulletin OEPP/EPPO Bulletin 45, 133– 138. Volume45, Issue2August 2015Pages 328-328 ReferencesRelatedInformation

Climate change increases the risk of invasion by the Yellow-legged hornet

The Yellow-legged hornet Vespa velutina nigrithorax has established viable populations in France since an unintentional introduction before 2004, and the potential invasion risk of this bee-hawking hornet was estimated by modelling the species climatic suitability worldwide. By further projecting such models under 2100 future climate scenarios, we here estimated the predicted trends in invasion risk related to climate change. We performed eight different modelling techniques within an ensemble forecast framework using presence data from the native and the invaded ranges. We predicted an increase in the climatic suitability for the species in the Northern hemisphere, especially close to the already invaded range in Europe, in Spain and in Central and Eastern Europe – from Switzerland to Hungary up to Southern Sweden. Climate change should also increase the invasion risk in the United States except along the Eastern coast. Scenarios for future climates include predictions from five global circulation models and three special reports on emission scenarios. Standard deviations of the results obtained from the 13 different climate scenarios confirmed the low uncertainty of models to predict an increase in invasion risk across Central and Eastern Europe, close to the already invaded European range. These regions hold among the highest densities of bee-hives in Europe, and could suffer from the potential predation of the putative invading hornet on pollinators.

Predicting the invasion risk by the alien bee-hawking Yellow-legged hornet Vespa velutina nigrithorax across Europe and other continents with niche models

Vespa velutina nigrithorax, an Asian bee-hawking hornet, has been unintentionally introduced in south-western France before 2004 and is currently widely spreading across the country. Its arrival in northern Spain was reported in 2010. The potential invasion risk of the species is assessed using climatic suitability models. We used eight different modelling techniques within an ensemble forecast framework to show that the invasion success in south-western France could have been predicted using data from the native Asian range of the species, while we further used data from both the native and invaded ranges (including a recently established population in Korea) to better predict its potential invasion range across all continents. Results are discussed in terms of the interest of ecological niche modelling for invasion biology, realised niche of the invasive wasp, potential threats to native entomofauna and economic impacts of this new predator. A particular attention is paid to beekeeping activities that are nowadays already threatened by a wide panel of adversary factors.