Intense Yellow Color Research Articles

Wpływ różnego udziału śruty bobikowej jako częściowego zamiennika poekstrakcyjnej śruty sojowej w mieszankach oraz czasu przechowywania w warunkach chłodniczych na cechy fizyczne mięsa kurcząt brojlerów

The study aimed at evaluating the effect of feeding broiler chickens with diets containing faba bean and cold storage time on the physical properties of m. pectoralis major. The study material comprised 24 muscles from chickens split into 3 feeding groups. Birds from the control group (I) were fed with compound feeds containing soybean meal as the only highprotein component, whereas the starter/grower diets administered to experimental chickens contained faba bean in the proportion 8/15% - group II and 16/22% - group III, as a partial substitute for soybean meal. No effect of the feeding method on acidity and water absorption capacity of the muscle was observed. Muscles of chickens fed with mixes with a higher share of faba bean were characterised by higher yellow saturation, colour intensity and hue in comparison to other groups. The storage time had no effect on their physical properties; a decrease was only recorded in pH24 of the evaluated muscles, which testifies to the correct course of glycolytic transformations. To sum up, it must be stated that a higher share of faba bean in chicken diets modifies muscle colour only, but cold storage time has no impact on the evaluated physical characteristics of muscles.

Medicinal Plants in East Sumba that Potential as Natural Dyes for Ikat Weaving

The revival of the natural dyes comes from an awareness of maintaining health and preserving the environment. Medicinal plants can be used as a source of the natural dyes. This study aimed to determine the medicinal plants in East Sumba Regency potentially exploited as a natural dye for ikat weaving. Plant data was obtained from Tana Tuku village and Mbatakapidu village in East Sumba Regency. A total 13 species of plants were extracted to dye cotton fabric and mordant by FeSO4 (ferrous sulphate), Al2(SO4)3 (alum) dan CaCO3 (lime). The intensity of color L*, a*, b* were analyzed by Kruskal Wallis test and Duncan post hoc test. The fastness of washing test analyzed by a gray color change scale and staining scale standard. The results showed that the ferrous sulphate mordant produced dark color and the alum mordant produced bright color. The intensity of red color was shown by Swietenia macropylla King and Leucaena leucocephala when it was mordanting by lime. The intensity of yellow color was shown by Woodfordia fruticosa (L.) Kurz with alum and Syzigium polyanthum with lime. The color change was not significant at 40 oC of washing shown by Tamarindus indica and Leucaena leucocephala mordanting by ferrous sulphate, Leucaena leucocephala, Bombax ceiba and Timonius timon mordanting by alum, Tamarindus indica, Leucaena leucocephala, Timonius timon mordanting with lime. All natural dyes did not stain the upholsteries.

Kinetics and mechanism of formation of the complex [Ru(CN)5INH]3− through the ligand substitution reaction between the aquapentacyanoruthenate(II) anion and isoniazid

The formation kinetics of the complex, [Ru(CN)5INH]3−, formed through the ligand substitution reaction between isoniazid (INH) and aquapentacyanoruthenate(II) ([Ru(CN)5H2O]3−), have been investigated, under pseudo first-order conditions, as a function of concentrations of [INH] and [Ru(CN)5H2O]3−, ionic strength and temperature at pH = 4.0 ± 0.02 in 0.2 M NaClO4 spectrophotometrically at 502 nm ( λmax of intense yellow colour product [Ru(CN)5INH]3−) corresponding to metal-to-ligand charge-transfer transitions, in aqueous medium. The pseudo first-order condition was maintained by taking at least 10% excess of [INH] over [Ru(CN)5H2O]3−. The stoichiometry of the reaction product was found to be 1:1 which was further supported and characterized using elemental analysis, infrared, nuclear magnetic resonance and mass spectrometric techniques. Thermodynamic and kinetic parameters have also been computed, using the Eyring equation, and the values of ΔH≠, Ea, ΔG≠ and ΔS≠ were found to be 47.3 kJ mol−1, 49.8 kJ mol−1, −8.62 kJ mol−1 and 187.6 J K−1mol−1, respectively. The reaction was found to obey first-order kinetics with respect to [INH]. It exhibited a negative salt effect on the rate upon variation of ionic strength of the medium. A tentative mechanistic scheme was proposed on the basis of experimental findings.

New series of brilliant yellow colorants in rare earth doped scheelite type oxides, (LiRE)1/2WO4- BiVO4 for cool roof applications

New series of brilliant yellow colorants with high NIR reflectance in rare earth doped scheelite type oxides, [(LiRE)0.125Bi0.75][W0·25V0.75]O4 (RE = La–Yb) and [(LiLa)x/2Bi1-x][WxV1-x]O4 (x = 0–0.3) were developed by the ceramic route. Different advanced techniques were used to examine the structural, morphological and optical properties of the synthesized colorants. The pigment series [(LiLa)x/2Bi1-x][WxV1-x]O4 undergoes a structural transformation from a monoclinic to a tetragonal scheelite phase and the second pigment series with various rare earth substitutions crystallize with a tetragonal phase. Strong absorption in the ultraviolet and blue region is observed for all the samples leading to brilliant yellow colorants with high solar reflectance. The band gap variation associated with the introduction of different energy levels between valance band and conduction band defines the various yellow shades of the pigments. Classically [(LiGd)0.125Bi0.75][W0·25V0.75]O4 pigment showed intense yellow color (b* 84.62) having NIR reflectance of 94% that has improved values than the commercially available Sicopal yellow. The probable applicability studies of [(LiLa)0·125Bi0.75][W0·25V0.75]O4 pigment as cool pigment was also exemplified on a concrete cement block and on a metal sheet. Chemical resistance tests are a sign of their permanence in the severe weathering conditions. These pigments are found to be promising ones for exterior coating energy saving applications.

Identification of genotoxic transformation products by effect-directed analysis with high-performance thin-layer chromatography and non-target screening

The w ide spread us e of the phar maceut ical met form in for diabete s therapy has led to finding its way into many surface waters up to the μg L-1 range and subsequently into different water treatment processes. In this study, metformin was treated with hypochlorite on the laboratory scale, and the resulting transformation products were investigated with the umu assay in a microtiter plate, where a genotoxic effect was detected. For the characterization of this genotoxic effect, the sample was separated using high-performance thin-layer chromatography (HPTLC), and 29 zones over the whole retardation area were extracted from the HPTLC plate with the thin-layer chromatography—mass spectrometry (TLC—MS) Interface. Then, the umu assay was performed again with each extracted zone, such that the genotoxic effect in the sample could be assigned to a certain zone. By the measurement of this effective zone with high-performance liquid chromatography with high-resolution mass spectrometry and by performing a non-target screening, the effective substance could be identified as a cyclic dehydro-1,2,4-triazole derivate with an intense yellow color. This substance formerly was found by Armbruster et al. (Water Research, 2015), which is a major transformation product of chlorine-treated metformin.

Relationship between quality characteristics and skin color of ‘Fuji’ Apples (Malus domestica Borkh.)

We investigated the relationship between apple skin color and internal quality characteristics, such as the concentration of functional compounds, physicochemical and textural properties. The skin color of ‘Fuji’ apples (N = 420) was determined and the apples were categorized into three classes (class A, N = 231; class B, N = 130; class C, N = 59). The apple skin color values correlated significantly with flesh color intensity. For sensory attributes, the yellow color intensity of apple flesh had a positive correlation with redness (a*, r = 0.781) and ΔE(r = 0.787) of apple flesh color (r = 0.787). There was strong correlation between a*, and the chemical properties of apple flesh. Apple skin color (a*) was positively correlated with total juice content (r = 0.784), soluble solid contents (r = 0.626), soluble solid contents/total acidity (r = 0.697), fructose (r = 0.444), sorbitol (r = 0.476), and sucrose concentrations (r = 0.823), respectively. However, malic acid (r = − 0.525), shikimic acid (r = − 0.443), and total organic acid concentrations (r = − 0.519) correlated negatively with a*. The a* value also showed positive correlations with phenol and anthocyanidine concentrations, but a negative correlation with DPPH, FRAP, and ABTS concentrations. A principal component analysis was performed to analyze functional compounds, sensory attributes, and physicochemical properties of apple skin and flesh; the total variance was 91.31% (F1; 58.92%, F2: 32.39%), 84.55% (F1;50.86%, F2;33.69%), 90.37% (F1;75.74%, F2; 14.63%) and 57.20% (F1;41.92%, F2;15.28%), respectively. Our results confirmed a strong correlation between the ΔE and a*, which are indicative of the skin color and the internal quality measures of the Fuji apple. Thus, the skin color of Fuji apples seems to be a reliable index of internal fruit quality.

Dissipation of Three Fungicides and Their Effects on Anthocyanins and Color of Monastrell Red Wines.

The effect of fungicides on fermentation is of paramount importance to control the quality and safety of wines. In this work, the quality (enological parameters, color, phenolic content, antioxidant activity, and fungicide residues) of wines from Monastrell grapes fortified with iprovalicarb, mepanipyrim, and tetraconazole fungicides was evaluated. Along the winemaking process, initial residues of mepanipyrim and tetraconazole were removed in more than 90% while the dissipation of iprovalicarb was around 73%. Significant statistical differences were found in the presence of iprovalicarb and mepanipyrim residues, especially at the highest concentration assayed. For both fungicides, increases in the volatile acidity (between 4 and 8.6 times), the lactic acid content (between 8.6 and 20.5 times), the percentage of polymeric anthocyanins (between 1.3 and 1.7 times), and also a slight increase of the total phenolic index and the total anthocyanin content determined by spectrophotometry were observed. On the contrary, the total monomeric anthocyanins content decreased about 16.3% and 28.6% in the presence of iprovalicarb and mepanipyrim, respectively. These results could be related to a higher development of acetic acid or lactic bacteria in the presence of these fungicides. The color of the final wines was also different in comparison with the control, with a higher yellow component, color intensity, tonality, and hue angle because of pH changes in the medium. Tetraconazole fermentations had a more similar trend to the control wine, probably due to the lower concentration of this fungicide in the grape must at the initial time. No effects on the antioxidant activity was observed for any of the target fungicides. A multivariate statistical analysis was done to view the interrelationships between different variables (color and anthocyanins profile). The obtained model allowed the wines to be separated according to the fungicide treatment applied.

Tetrahydrocurcumin-loaded vaginal nanomicrobicide for prophylaxis of HIV/AIDS: in silico study, formulation development, and in vitro evaluation.

A vaginal microbicide is a front-line women-dependent approach and an alternative to a condom for prevention of unprotected sexual intercourse-associated HIV. The microbicide research is still in its infancy with several products in the clinical studies being reported to have good efficacy, safe, but with poor adherence. One such molecule reported with an excellent efficacy when tested preclinically is curcumin, a natural polyphenol derived from Curcuma longa. Despite its potential HIV-1 inhibitory activity, it has intense yellow color staining properties, which would result in poor consumer compliance and adherence for vaginal application. To address this issue, tetrahydrocurcumin (THC), a colorless derivative of curcumin, was subjected to in silico screening (molecular docking and dynamics simulation studies) using homology model of gp120-CD4 binding. It was found that THC exhibited equivalent gp120-CD4 binding inhibitory activity as compared with curcumin due to its stable hydrophobic interactions with residues Asp368 and Trp427 deeper in the Phe43 cavity of CD4 receptor. Hence, it can be effectively used as a potential microbicide candidate. THC, a BCS Class II molecule exhibits poor solubility, spreadability, and intracellular uptake when used in the conventional form. Thus, it was decided to develop a lipid-based nanomicrobicide gel for delivery of THC. The developed THC-loaded o/w microemulsion gel was characterized for physicochemical properties (globule size, drug content, drug release, and permeation) and further used for in vitro cell line studies (cell viability, cellular uptake, and anti-HIV activity). The developed formulation was found to be stable with coitus-independent release profile and exhibited a rapid time-independent intracellular uptake. In addition, it exhibited a fourfold increase in efficacy as compared with conventional THC. Thus, the novel THC-loaded o/w microemulsion gel exhibited the potential for prevention of HIV-1 infection associated with unprotected sexual intercourse.

Early- and middle-phase arterial repair following bioresorbable- and durable-polymer drug-eluting stent implantation: An angioscopic study

BackgroundBioresorbable-polymer drug-eluting stent (BP-DES) demonstrates comparable clinical outcomes compared to durable-polymer drug-eluting stent (DP-DES). However, early- and middle-phase arterial repair following deployment of BP-DES and DP-DES has not been elucidated to date. MethodsWe extracted coronary angioscopy (CAS) findings covering early phase (4 ± 1 months) or middle phase (10 ± 2 months) between January 2010 and February 2018 from the database of Kansai Rosai Hospital. Neointimal coverage (NIC), yellow color intensity of the stented segment and incidence of thrombus adhesion were compared between BP-DES (Synergy or Ultimaster) and DP-DES (Promus or Resolute or Xience) in early (39 BP-DES of 33 lesions from 24 patients and 83 DP-DES of 74 lesions from 56 patients) and middle (198 BP-DES of 175 lesions from 135 patients and 204 DP-DES of 184 lesions from 149 patients) phases. ResultsIn early phase, while NIC was similar in both groups (P = 0.84), the incidence of thrombus adhesion was significantly higher in BP-DES than in DP-DES (67% versus 34%, P = 0.001) even though maximum yellow color was less intense in BP-DES (P = 0.004). In middle phase, while NIC was better in BP-DES (P < 0.001), thrombus adhesion (23% versus 22%, P = 0.81) and maximum yellow color (P = 0.72) were similar in both groups. ConclusionsAlthough NIC was similar in the early phase, the incidence of thrombus adhesion was significantly higher in BP-DES than in DP-DES. The incidence of thrombus adhesion reached similar values and NIC improved in BP-DES over that in DP-DES in the middle phase.

Smartphone-based Ellman’s colourimetric methods for the analysis of d-penicillamine formulation and thiolated polymer

A new, smartphone-based colourimetric method for the assay of d-penicillamine formulations relying on the Ellman’s reaction was developed by performing the colourimetric reaction in a microplate. Subsequently, the plate was positioned on a white illuminating screen of an iPad placed in a dark box in order to capture a top-view image using an iPhone 5s back camera. The intensity of yellow colour was converted to Red–Green–Blue pixels using a free mobile application. Under the optimal conditions for the reaction and photography, the intensity of blue colour, which was logarithmically transformed, showed an excellent linearity over the drug concentration range of 5–40 µg/mL. The assay was validated and successfully applied to the assay of drug content and the determination of drug amount released in the dissolution test in the capsule dosage forms. Apart from that, a smartphone was employed for the colour measurement as an alternative to a spectrophotometer in the currently used method for the quantitation of free sulfhydryl groups in polymers. Using cysteine-conjugated chitosan as a sample and l-cysteine as a standard, the smartphone method gave the results in agreement with those obtained from the absorbance measurement on a microplate reader. In conclusion, smartphone-based colourimetry has been proved to be a reliable, fast, simple and affordable alternative means for the analysis of d-penicillamine and cysteine-conjugated polymer and can be potentially applied to other thiol-containing drugs and excipients.

Qualidade da carne de tambaqui abatido com diferentes classes de pesos

The experiment was conducted to evaluate the meat quality of tambaqui slaughtered at different weights. Twenty-four animals were slaughtered at body weights ranging from 1.0 to 3.5 kg. The slaughter weight did not affect meat pH (6.42), luminosity (L*), red color intensity (a*) or yellow color intensity (b*) (means of 62.0, 2.86 and 15.57, respectively), nor the sensory attributes appearance (7.41), flavor (7.25), texture (7.40), and overall acceptance (7.24). Weight loss by dripping and defrosting and the water holding capacity of tambaqui meat were affected (P<0.01) by slaughter weight. Although a slaughter weight of tambaqui between 1.0 and 1.5 kg was associated with further water loss, the other meat quality traits were not compromised.

A comparison of antioxidant and Fourier Transform Infrared Spectroscopy (FTIR) analysis on extracts of Syzygium guineenses (Myrtaceae)

Background: Syzygium guineenses, (the most common and abundant specie in Nigeria) is a medicinal plant used by traditional practitioners in northern Nigeria for a variety of healing purposes. Objective: The main objective of this project was to carry out a comparison of antioxidant activities and Fourier Transform Infrared Spectrophotometric (FTIR) analysis on both methanol and hexane leaf extracts of S. guineenses. Methods: Phytochemical screening, Semi-quantitative DPPH (1,1-diphenyl-2-picrylhydrazyl)- dot blot assay and FTIR analysis were performed on both extracts to determine antioxidant activity and identify the functional groups present. Results: Phytochemicals tested for, were observed to be more prominent in the methanol extract than hexane. The in vitro antioxidant assay also revealed a more intense yellow colour of inhibition in methanol extract than the hexane extract. The FTIR spectra revealed different characteristic peak values with various functional compounds in both extracts. The methanol extract displayed major peaks of absorption at 3341 cm-1 (-OH) for alcohol, 1736 cm-1 (C=O) carbonyl group, 1161.83 cm-1, 1036.49 cm-1 (C-O) of esters. Other absorption bands like 1452.25 cm-1 and 1612.20 cm-1 for alkenes were present in both extracts. Conclusion: This result shows that the methanol extract of S. guineenseshas a higher potential of phytochemicals, antioxidants and functional groups than the hexane extract.

Intelligent Chitosan/PVA nanocomposite films containing black carrot anthocyanin and bentonite nanoclays with improved mechanical, thermal and antibacterial properties

This study investigated the potential of chitosan/PVA films containing black carrot anthocyanins as a natural pH indicator and bentonite as a nano-filler, for intelligent packaging applications. Two series of chitosan/PVA (CP) films, with different concentrations of bentonite (CPB3%, CPB5%) and with a constant concentration of anthocyanin (CPA, CPAB3%, CPAB5%) were prepared by solution casting method. The colorimetric parameter b* showed an increase from 1.0 ± 0.2 for CP films to 2.7 ± 0.2 and 8.8 ± 1.1 for CPB3% and CPB5% films, respectively which indicates that addition of bentonite increases the intensity of yellow color in the nanocomposite films. The presence of anthocyanins caused to shifting of a* values from negative to positive values which indicated the color change of films from greenness to redness. Addition of bentonite reduced the tensile strength from 100.6 for CP film to 86.5 and 67.2 for CPB3% and CPB5% films which was related to the solution intercalation method used for preparation of nanocomposite films. In contrast, addition of anthocyanins increased the tensile strength for CPA, CPAB3% and CPAB5% due to the hydrogen bonding interactions between its OH groups and PVA and chitosan chains. Water vapor permeability (WVP) of the films was decreased from 1.140 (mg.mm/m2.kPa.h) for CP film to 0.5620 and 0.3515 for CPB3% and CPB5% films, respectively. In contrast, anthocyanins caused to increase WVP of the samples compared to the anthocyanin free films. Thermo-gravimetric analysis showed that the films containing bentonite and anthocyanins have a better thermal stability. Finally, CPAB5% showed the highest bacterial growth inhibition against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, respectively 75.20%, 69.95% and 44.38%.

Orange-fleshed sweet potato flour as a precursor of aroma and color of sourdough panettones

The nutrients and the color profile of orange-fleshed sweet potato flour (SPF) can contribute positively with the long fermentation products. This study aimed to evaluate the effect of the substitution wheat flour (WF) by SPF in the sponge and dough phases on the quality (moisture and color) and flavor profile of sourdough panettone. Panettone was obtained using spontaneous sourdough and long fermentation time (23 h). Four formulations were made, as follows: AP1 (control), AP2 (10 g/100 g SPF in dough phase or last fermentation), BP3 (10 g/100 g of SPF in sponge phase or intermediary fermentation), and BP4 (10 g/100 g SPF in each phase, sponge and dough phase). The formulation BP4 had a more moist crumb, a high yellow color intensity, and a smaller specific volume (3.26 cm3/g) when compared to the control (3.76 cm3/g). Regarding the volatile compounds, three compounds were identified in the panettones made with SPF: 2-octenal-2-butyl, dimethyl-decane, and 2-chlorooctane, which were not found in control. These results can contribute to further studies about the use of SPF as a precursor of new flavor compounds in long fermentation times bakery products, thus reducing or eliminating the use of aromatic additives and food colorings.

Effects of organic and conventional farming on the physicochemical and functional properties of jujube fruit

Organic food is associated with improved nutritional properties, and this consumer expectation has led to increasing demand for organic fruits and vegetables. The objective of this study was to evaluate the changes in physical, chemical, and nutraceutical parameters of the jujube fruits 'Grande de Albatera' cultivar, grown under organic or conventional production systems. Results showed that the organic jujubes were smaller, with a slightly more intense yellow and red color, with higher contents of chlorophylls, carotenoids, sugars, organic acids, and total volatile compounds, but with lower protein and flavonoids, contents than conventional jujubes. Therefore, it can be concluded that the market quality of organic jujubes was similar to that of the conventional ones because fruits were smaller but with a more intense coloration. However, the flavor quality was better as they had more sugars, acids, and volatile compounds, making the flavor of fruits more attractive for consumers. Finally, there were no significant differences in the antioxidant attributes, as organic and conventional jujubes had similar total contents of phenols and antioxidant activity.

Wet agglomeration by high shear of binary mixtures of curcumin-loaded lyophilized liposomes and cornstarch: Powder characterization and incorporation in cakes

Wet agglomeration was used to enrich cornstarch with curcumin-loaded lyophilized liposomes, and the enriched cornstarch was incorporated in cake batters. Lyophilized liposomes were obtained using proliposome hydration and encapsulated curcumin remained stable after 70 days of storage. The enriched cornstarch was produced using a high shear method using maltodextrin as a binder, and the powders were characterized in terms of water activity, moisture content, water sorption, bulk properties, flowability, wetting time, instrumental colorimetry, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. The data obtained showed that the presence of liposomes and maltodextrin did not significantly change the cornstarch bulk structure. The enriched powders were homogeneous in both color and size distribution. Better bulk properties were observed in agglomerated powders than in non-agglomerated cornstarch, as higher flowability and lower cohesiveness. With respect to the application of the agglomerated starches in cakes, they were produced and analyzed in terms of instrumental color, texture and proximate composition. The results showed a decrease in both hardness and chewiness as well as an intense and homogeneous yellow color in cakes produced with the agglomerated cornstarch. This study indicated that it is feasible to produce a cake batter with curcumin encapsulated in liposomes as a functional ingredient.

Effect of Xanthan Gum/Soybean Fiber Ratio in the Batter on Oil Absorption and Quality Attributes of Fried Breaded Fish Nuggets.

This study clearly showed that the combined addition of XG and SF at different ratios in the batter significantly affected fat content and quality attributes of fried BBFNs. The inhibition of oil absorption and improvement of color and textural characteristics in fried BBFNs depended on the XG/SF ratio added to the batter, and a 1:2 w/w ratio was found to produce the maximum enhancement.

Serving Duplicates in a Single Session Can Selectively Improve Sensitivity of Duplicated Intensity Ranking Tests.

This study evaluated two alternative and not previously studied protocols for conducting a duplicated ranking test. One protocol required that each panelist received duplicates in the same single serving session (1SS), against the alternative of serving duplicates separately in two sessions to allow a break period (2SS). Two attributes: yellow color intensity and sweetness intensity of orange juice samples were studied. This study showed that serving duplicates in the same single session can improve duplicated ranking's power, depending on the task difficulty and attribute, and should be considered before splitting replications into two sessions.

Biogenic gold nanoparticles for reduction of 4-nitrophenol to 4-aminophenol: an eco-friendly bioremediation.

The present study investigated the synthesis of gold nanoparticles (AuNPs) using mangrove plant extract from Avicennia marina as bioreductant for eco-friendly bioremediation of 4-nitrophenol (4-NP). The AuNPs synthesised were confirmed by UV spectrum, transmission electron microscopy (TEM), X-ray diffraction, Fourier transmission infrared spectroscopy (FTIR), dynamic light scattering (DLS), and zeta potential. The AuNPs were found to be spherical in shape with size ranging from 4 to 13 nm, as evident by TEM and DLS. Further, the AuNPs were encapsulated with sodium alginate in the form of gold nano beads and used as heterogeneous catalyst and degrading agent to reduce 4-NP. This reduction in 4-NP into 4-aminophenol was confirmed by UV and FTIR. The aqueous solution of 4-NP peaked its absorbance at 320 nm, and shifted to 400 nm, with an intense yellow colour, appeared due to formation of 4-nitrophenolate ion. After the addition of AuNps, the 4-NP solution became colourless and peaked at 400 nm and reduced to 290 nm corresponding to the formation of 4-aminophenol. Hence, the present work suggested the AuNPs as the potent, eco-friendly bionanocomposite catalyst for bioremediation of 4-NP.

Morphological characterization of fruit, seed and seedling, and seed germination test of Campomanesia guazumifolia

Abstract: Campomanesia guazumifolia (Cambess.) O. Berg. is a native fruit species, with ecological importance and potential to be exploited by food and medical industries. There is a lack of studies enabling its on-site conservation and propagation for commercial purposes. The objective of this work was to morphologically characterize the fruit, seed and seedling development stage of this species, as well as to determine an efficient protocol for the germination test, aiming at the commercial production of seedlings. Different temperatures (20, 25, 30 and 20-30 °C), substrates (blotting paper, sand and vermiculite) and light regimes (presence and absence) were tested in order to evaluate the germination power of seeds. Fruits are bacoid, rounded, with a fleshy pericarp and hairy surface, and with an intense yellow coloration. Seeds have a reniform shape and a lateral recess, with a membranous, brown and verrucose-glanulose tegument. It has a crass embryo, with a well developed hypocotyl-radicle axis and vestigial cotyledons. Seedlings are epigeal and phanerocotylar. It is recommended to conduct the germination test for the species with temperatures of 25 or 30 ºC, on paper substrates, with light presence or absence, and with the first test count performed 23 days and the final count 50 days after sowing.