Medicinal plants are a type of plant that partially or completely and the exudate of the plant is used as medicine, medicinal ingredients, or medicinal ingredients. One of the plants that has been used by the community is Baban dotan (Ageratum conyzoides Linn.) which is one of the superior medicinal plants that has multifunctional properties. This study aims to determine the content of compounds contained in the Babandotan plant (Ageratum conyzodes Linn.) and analysis of the alkaloid content using the Thin Layer Chromatography Method. In the phytochemical screening examination of baban dotan (Ageratum conyzoides Linn.) plants. Examination of the analysis of alkaloids using the Thin Layer Chromatography method resulted in the results of the active substances in the Baban dotan plant in the form of alkaloid content separating or being attracted from the focal point and obtained 2 spots that appeared with a brownish yellow color. The results showed that in the phytochemical screening and alkaloid analysis of the Babandotan plant (Ageratum conyzoides Linn.) using the Thin Layer Chromatography method, there were characteristics that could be used to identify the plant.