Yeast Candida Utilis Research Articles

Effects of an adenine analog on yeast metabolism

An adenine analog, 6-methylpurine, has been found to inhibit growth and synthesis in the yeast Candida utilis. The analog inhibits the synthesis of guanylic acid from adenylic acid, depresses the incorporation of adenine in ribonucleic acid, inhibits ribosomal synthesis, and leads to an altered distribution of amino acids in the cellular metabolic pool. 6-Methylpurine appears to act in this organism in a similar manner to 5-fluorouracil.

Effects of an adenine analog on yeast metabolism

An adenine analog, 6-methylpurine, has been found to inhibit growth and synthesis in the yeast Candida utilis . The analog inhibits the synthesis of guanylic acid from adenylic acid, depresses the incorporation of adenine in ribonucleic acid, inhibits ribosomal synthesis, and leads to an altered distribution of amino acids in the cellular metabolic pool. 6-Methylpurine appears to act in this organism in a similar manner to 5-fluorouracil.

Effects of an adenine analog on yeast metabolism

An adenine analog, 6-methylpurine, has been found to inhibit growth and synthesis in the yeast Candida utilis. The analog inhibits the synthesis of guanylic acid from adenylic acid, depresses the incorporation of adenine in ribonucleic acid, inhibits ribosomal synthesis, and leads to an altered distribution of amino acids in the cellular metabolic pool. 6-Methylpurine appears to act in this organism in a similar manner to 5-fluorouracil.

Formotion and metabolism of S-adenosyl- l-homocysteine in yeast

The formation of S-adenosyl- l-homocysteine and of S-adenosyl- l-methionine has been studied in the yeasts Candida utilis and Saccharomyces cerervisiae. Evidence for the formation of S-adenosyl- l-homocysteine from adenosine and l-homocysteine was obtained. When S-adenosyl- l-homocysteine, labeled with C 14 in the adenine moiety, was added to the culture medium, labeled S-adenosyl- l-methionine could be isolated from the cells. The conversion appears to involve the attachment of a methyl group or methyl precursor directly to the sulfur atom of S-adenosyl- l-homocysteine.

Spheroplasts of the yeast Candida utilis.

Svihla, G. (Argonne National Laboratory, Argonne, Ill.), F. Schlenk, and J. L. Dainko. Spheroplasts of the yeast Candida utilis. J. Bacteriol. 82:808-814. 1961.-The formation and properties of spheroplasts of the yeast Candida utilis, produced by digestion of the cell walls with snail gut juice (Helix pomatia) in isotonic medium, were studied by phase, interference, and ultraviolet microscopy, and tracer techniques. Cells cultivated in the presence of l-methionine, S-methyl-l-methionine, and l-homocysteine (5 mumoles/ml) were more susceptible to enzyme action than ordinary cells. These cells contain increased amounts of S-adenosylmethionine in the vacuole which facilitates ultraviolet microscopy. Spheroplasts are stable in 0.6 m KCl containing 0.04 m phosphate, but are disintegrated by water, sonic vibration, or ultraviolet irradiation. Exposure in isotonic medium to 6 x 10(4) erg/mm(2) energy at 253 mmu breaks the cytoplasmic membrane and liberates the vacuole with membrane intact. The vacuolar membrane may be broken with about three times the initial dose of ultraviolet.

Adenine metabolism in [formula omitted] adenase from bakers' yeast

Di Carlo, Schultz, and McManus (1951) provided evidence for the presence of adenase in the yeasts Candida ( torula ) utilis and Saccharomyces cerevisiae . Roush (1954) prepared extracts of adenine-induced C. utilis that contained an adenase but found that extensive fractionation was required to separate the adenase from accompanying guanase and uricase ( Roush, 1958). Heppel, Hurwitz, and Horecker (1957) obtained a highly purified adenase from Azotobacter vinelandii but the adenase in the crude extracts was accompanied by adenosine deaminase and several fractionation steps were required to separate the two activities. This paper reports an induced adenase in S. cerevisiae . The crude enzyme is practically free of enzymes acting on many purine and pyrimidine compounds other than adenine and it is obtained easily from commercially available fresh or active dry bakers' yeast.

Comparison of Dried Yeasts, Candida Utilis and Saccharomyces Cerevisiae, as Supplements in a Diet for Rainbow Trout

A single grade of the yeast Saccharomyces cerevisiae and three grades of the yeast Candida utilis were tested as growth stimulants and as vitamin sources when fed in a dry pelleted diet to rainbow trout. At levels of 4 percent, 8 percent and 15 percent of dry yeast in the diet, growth was stimulated and conversion ratios improved. Maximum growth occurred on 8-percent yeast diets. With the exception of one grade of C. utilis, all yeasts at the same levels in the diet gave similar results. Vitamin analyses indicated that diets enriched with 4-percent of yeast were deficient in meeting the daily requirement levels of B-vitamins. Because no appreciable mortality occurred after feeding these diets for 18 weeks, it is suggested that reported daily requirements are high. Storage of diets at room temperature for 180 days had little destructive effect on B-vitamin content, except some loss in niacin occurred. A commercial pelleting process effected no loss in B-vitamin content. The mortalities of trout on yeast-fortified diets and the yeast-free diet were similar. Liver necrosis in trout on the diets for 33 weeks appeared to a like degree for all diets tested. At similar levels in the diet, C. utilis yeasts promoted higher storage levels of biotin. Trout on 8-percent yeast diets had less liver biotin than those on 4-percent yeast, 15-percent yeast and yeast-free diets. When trout are fed a pelleted diet at 60 percent of that recommended in Cortland Feeding Chart, they attained a coefficient of condition, empirically expressed as “R”, of 1.80 to 1.86 on the yeast supplemented diets and of 1.73 on the yeast-free diet.