Ecological degradation has occurred in global grasslands and has impaired their ecosystem services severely, so ecological conservation of grasslands should be focused more on the effectiveness of management measures. The green-up process decides the year-round forage yield and ecological conditions of grasslands. Adopting rest-grazing during the green-up process can guarantee a successful green-up, thus realizing more economic benefits without grassland degradation. Therefore, studies should pay more attention to whether the green-up process is really covered by the rest-grazing period or not. We analyze the spatiotemporal variations and the stability of the annual green-up date in Xilin Gol Grassland from 2000 to 2018 based on MODIS time series images and compare the green-up date with the rest-grazing period to assess the effectiveness of the rest-grazing policy. The results show that the green-up date of Xilin Gol Grassland had advanced 15 days on average because of the increasing trend of both temperature and precipitation during 2000~2018. The green-up date is mostly 120~130 d in the east, about 10 days earlier than the west (130~140 d) and 20 days earlier than in the central areas (140~150 d), also because of the spatial variations of temperature and precipitation. The coefficient of variation (CV) of the green-up date showed a significant negative correlation with precipitation, so the green-up date is more unstable in the arid areas. The rest-grazing period started more than 45 days earlier than the green-up date and failed to cover it in several years, which occurred more frequently in southern counties. The average green-up date appeared after rest-grazing started in over 98% of areas, and the time gap is 15~45 days in 88% of areas, which not only could not avoid grassland degradation effectively but also increased herdsmen’s life burden. This study aims to accurately grasp the temporal and spatial variations of the green-up date in order to provide references for adjusting a more proper rest-grazing period, thus promoting ecological conservation and sustainable development of animal husbandry.