Wound Healing Patch Research Articles

Novel nitric oxide producing probiotic wound healing patch: preparation and in vivo analysis in a New Zealand white rabbit model of ischaemic and infected wounds

The treatment of chronic wounds poses a significant challenge for clinicians and patients alike. Here we report design and preclinical efficacy of a novel nitric oxide gas (gNO)-producing probiotic patch for wound healing. Specifically, a wound healing patch using lactic acid bacteria in an adhesive gas permeable membrane has been designed and investigated for treating ischaemic and infected full-thickness dermal wounds in a New Zealand white rabbit model for ischaemic wound healing. Kaplan-Meier survival curves showed increased wound closure with gNO-producing patch-treated wounds over 21 days of therapy (log-rank P = 0·0225 and Wilcoxon P = 0·0113). Cox proportional hazard regression showed that gNO-producing patch-treated wounds were 2·52 times more likely to close compared with control patches (hazard P = 0·0375, score P = 0·032 and likelihood ratio P = 0·0355), and histological analysis showed improved wound healing in gNO-producing patch-treated animals. This study may provide an effective, safe and less costly alternative for treating chronic wounds.

English

In this study, the wound healing activity of total aqueous extract and the mucilage of leaves of plant Cocculus hirsutus (L) Diels was evaluated on excision wound model. Dermal films were prepared using total aqueous extract, mucilage and polyvinyl alcohol as a polymer base. The prepared polysaccharide solutions of the total extract and the isolated mucilage were evaluated for its physiochemical properties and total bacterial count. The prepared dermal patches were subjected biodegradation studies. The results indicate statistically significant wound healing response of the dermal patch containing the aqueous extract and the mucilage of leaves of plant C. hirsutus when compared with control group and groups subjected to standard marketed medicated dermal patch. The cost effective factor and biodegradation of the prepared dermal wound healing patch was also found to be significant.   Key words: Cocculus hirsutus (L.) Diels, wound healing, excision wound model, Dermal patches.