Wound Healing Ointment Research Articles

Wound healing characteristics of a novel wound healing ointment in an abrasive wound model: A randomised, intra-individual clinical investigation

Moist wound healing conditions are beneficial in a professional wound care setting as well as for self-treatment of acute, superficial wounds. The purpose of this randomized, controlled, investigator-blinded investigation was to determine the local tolerability, wound healing efficacy and cosmetic outcome of a novel wound healing ointment in an intra-individual comparison of 4 treatment regimens in an abrasive wound model. Standardized abrasive wounds were induced on the inner forearms of 30 healthy subjects and 4 treatment regimens were randomly allocated to test areas (wound healing ointment covered with standard first aid dressing, wound healing ointment covered with gauze, standard first aid dressing alone, untreated area covered with gauze). Wounds were treated once daily for 11 days. Local tolerability and wound healing were assessed using visual scoring and digital photography on 5 different days. The cosmetic outcome was evaluated on a follow-up visit on Day 31. The wound healing ointment exhibited excellent local tolerability with superior assessments in comparison to treatment utilizing only dressings without ointment. Significant differences between AUC values for re-epithelization and overall wound healing efficacy were demonstrated in favor of treatment with the wound healing ointment in comparison to dry wound healing conditions. Wounds treated with the wound healing ointment showed a faster onset of healing and the cosmetic outcome was rated as being superior for the wound healing ointment both by the investigator and the subject. Superficial cutaneous wounds treated with the novel wound healing ointment displayed a significant improvement of wound healing with an earlier onset of re-epithelization, faster wound closure and a better cosmetic outcome. Clinically relevant accelerated wound healing compared to traditional dry healing could be shown demonstrating the benefits of moist wound healing conditions also in the treatment of minor, superficial wounds (Clinical trial identification number: EUDAMED_CIV 17-04-019364).

Pharmaceutical development of complex wound-healing ointment for the needs of military medicine

This article presents experimentally proved scientific-practical methods of developing modern vulnerary medical preparations in the form of an ointment with antibacterial, anti-inflammatory and anesthetic properties for the purposes of military medicine. In pursuit of the designated goals, the authors used modern methods of research: pharmacological-technological, physical-chemical, structural-mechanical and biological methods – for proving the compound of the combined ointment including ofloxacin, nimesulide and lidocaine. On the basis of the research conducted, we proved the content and rational technique of the complex antimicrobial ointment with anti-inflammatory and anesthetic effect using ofloxacin (0.05 g), nimesulide (1.0 g) and lidocaine (4.0 g). We experimentally established the optimal content of the main component of the ointment: emulsive base of mixture of Polyethylene Oxide-400 and Polyethylene Oxide-1500 (1 : 4) and a complex of emulsifiers of the first and the second generations – cetostearylic alcohol – 3%, monoglyceryl stearate – 5% at the constant concentration of paraffin oil – 20%, which involves a certain temperature regime during preparation, a sequence of introducing active pharmacological ingredients and adjuvants to the water and oil phases, frequency and duration of mixing. Physical-chemical tests of the ointment base determined the optimum concentration of glycerine as 5%, which encourages elimination of purulent discharge from damaged tissues, causing an anti-inflammatory effect and providing a prolonged, but milder dehydrating effect. The relation between physical-chemical, rheological, biopharmaceutical properties of the developed preparation and the concentration and method of introduction of the active pharmaceutical ingredients was theoretically and experimentally proven. We conducted pre-clinical studies (in vivo) for defining the therapeutic efficiency of the ofloxacin-nimesulide composition in the compound of the developed ointment for complex treatment of the wound process. We studied the specific activity (anti-alternative – model of standard tissue wounds in rats), which allowed us to establish the optimum concentration of nimesulide, which is 1%. Biopharmaceutical studies (in vitro method) determined the optimum method of introducing ofloxacin, nimesulide and lidocaine in the ointment base as a solution in dimethyl sulfoxide. To select an anesthetic substance for the ointment, the authors conducted a comparative study on the anesthetic effect of the anesthetics most widely available in Ukraine: novocaine, dicainum, lidocaine, trimecainum and pyromecainum. The research conducted on the time it took for the anesthetic effect to occur and on the duration and extent of the effect enabled us to select a 4% concentration of lidocaine anesthetic for the preparation. The choice of the temperature regime for the ointment’s preparation was proved. The in vitro method and rheological studies determined that the optimum temperature regime for the technological process of preparing the ointment on the emulsive base is 25–30 ºС. The development and introduction of the new combined ointment on a contemporary hydrophilic ointment base into practical pharmacy would lead to an enlargement in the nomenclature of the medical preparations used for local treatment of the purulent – necrotic phase of the wound process, which is relevant for pharmaceutical technology and global medical science and practice.

Influence of a new wound-healing ointment on morphogenesis of full-thickness excision wounds in healthy rats and rats with diabetic state.

Background. Impaired wound healing is a complication of diabetic state and is a serious medical and social problem. It occurs as a consequence of disequilibrium in reactive oxygen species (ROS) and antioxidant defense system. Therefore local treatment with antioxidants is promising in this type of pathology. Objective. The aim of this study was morphological evaluation of the influence of a new wound-healing ointment containing licorice extract, coenzyme Q10 and dihydroquercetin on the model of natural and delayed wounds in rats. Methods. For morphological evaluation on Day 6 and 12 we took specimens of tissues with wound and surrounding intact skin. Prepared histological slides and stained with haematoxylin, eosin and picrofuchsin by Van Gieson. Results. It was noted that process of excision wound healing in rats with diabetic state had prolonged inflammatory phase. Thus, on the Day 6 of observation during wound-healing in a physiologically healthy rats fibrinoid-necrotic layer in the surface layers of the defect was quite dense, with a limited number of leukocyte cells. Young newly formed granulation tissue in the bottom areas also purified from neutrophil leukocytes. Inflammatory reaction in the boundary to the defect areas of skin was extinguished. And in rats with diabetic state same fibrinoid-necrotic layer was markedly attenuated, full of polymorphonuclear leukocytes, granulation cells also contained many white blood cells in the boundary areas of healthy skin observed exocytosis of epithelial layer, a marked inflammation and destruction of the dermis. All this led to decrease in speed of development and maturation of granulation tissue on the 12th day of observation in these animals compared with physiologically healthy rats. Conclusion. The results showed that the studied ointment optimizes reparative tissue regeneration in the healing of skin excision wounds in rats with pre-modeled diabetogenic state. Nature of reparative processes in these rats generally followed that of the physiologically healthy rats.

Evaluation of wound healing activity of Acacia leucophloea bark in rats

Wound healing activity of the bark extracts of Acacia leucophloea Willd., Fabaceae, was investigated by excision and incision wound healing models in Wistar male rats. Ethanolic extract based ointment of A. leucophloea bark (2 and 5% (w/w)) was formulated and evaluated for its wound healing in Wistar male rats. In comparision with a standard wound healing ointment betadine. A. leucophloea ethanolic extract ointment exhibited marked wound healing activity and significantly enhanced the wound contraction and the period of epithelialization as assessed by wound contraction rate, tensile strength, increasing of DNA, collagen and protein synthesis and histopathological examination. The formulated ointment might well find use as skin repair agent without hazard to human health based on these results.

A novel wound healing ointment: A formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge

Hypericum perforatum L. (Hypericaceae), olive oil (Oleaceae), Origanum Tourn ex L. and Salvia L. species (Lamiaceae) are used against inflammatory disorders and for healing of skin wounds in traditional Turkish medicine. A new ointment formulation was developed to provide more efficient wound healing activity. The content of the formulation was as follows; olive oil extract of flowering aerial parts of Hypericum perforatum L., olive oil, an equivalent mixture of Origanum majorana L. and Origanum minutiflorum Schwrd. et Davis essential oils (Origani aetheroleum), Salvia triloba L. essential oil. The aim of the present study is to assess the wound healing potential of this new formulation by using in vivo and in vitro models as well as histopathological methods. For the evaluation of wound healing potential of this formulation (HPP crème mit Rotöl ointment) in vivo wound healing experimental models were employed on rats and mice and the efficiency was comparatively assessed against a reference ointment Madecassol(®). Tissue sections were also evaluated histopathologically. Furthermore, the wound healing activity of each component was also investigated individually to determine the improvement in the healing capacity of the formulation. The ointments of HPP crème mit Rotöl and Hypericum perforatum L. demonstrated the highest activities on both wound models when compared to reference ointment Madecassol(®), while the other ingredients did not show any remarkable wound healing effect. However, the efficacy of the formulation was remarkably higher than the Hypericum L. ointment alone which was also confirmed by histopathological evaluation. On the other hand, the formulation did not reduce elastase activity in vitro, but inhibited the collagenase activity. HPP crème mit Rotöl also exerted bactericidal and candicidal activities. The experimental studies revealed that HPP crème mit Rotöl formulation displays remarkable wound healing activity. To be acting on the different stages of wound healing process could be considered as a beneficial effect of the formulation for the treatment of wounds.

Hametum®Wund- und Heilsalbe bei arzneimittelinduzierten Hautsymptomen

Hintergrund/Ziel: Unter Anwendung von systemisch verabreichten Medikamenten treten häufig Hautsymptome wie Juckreiz, Rauheit, Spannungsgefühl, Schuppung und feine Hautrisse als unerwünschte Nebenwirkung auf. Ziel dieser Studie war es deshalb, die Wirksamkeit und Verträglichkeit von Hametum® Wund- und Heilsalbe (Wirkstoff: Hamamelisdestillat) bei Patienten mit arzneimittelinduzierten Hautsymptomen zu untersuchen. Methoden: Im Rahmen dieser monozentrischen, offenen Phase-IV-Studie wurde das Prüfpräparat über 28 Tage zweimal täglich auf die betroffenen Hautstellen aufgetragen (Prüfareal: linker Unterschenkel). Primäre Zielgrößen waren Hautfettgehalt (Messung per Sebumetrie) und Hautfeuchtigkeit (Messung per Corneometrie). Ergebnisse: Der Hautfettgehalt erhöhte sich bis zum Behandlungsende signifikant von 0,69 ± 0,99 auf 60,91 ± 76,86 µg Sebum/cm2 Haut (p < 0,001; t-Test für verbundene Stichproben). Bezüglich der Hautfeuchtigkeit zeigte sich eine tendenzielle Zunahme von 43,53 ± 8,02 auf 45,12 ± 13,16 arbitrary units (p = 0,218; t-Test für verbundene Stichproben). Die Hautverträglichkeit wurde vom Prüfarzt in 97,2% der Fälle mit »sehr gut« oder »gut« bewertet. Schlussfolgerungen: In dieser Studie konnte gezeigt werden, dass Hametum® Wund- und Heilsalbe zur Behandlung der arzneimittelinduzierten Hauttrockenheit sehr gut geeignet ist und eine sehr gute Hautverträglichkeit aufweist.

Synthesis of 2-butyl-4,9-decadienoic and 2-butyl-4,9-dimethyl-4,9-decadienoic acids, structural analogues of cygerol

2-Butyl-4,9-decadienoic and 2-butyl-4,9-dimethyl-4,9-decadienoic acids, structural isomers of 2-cyclohexylgeranylacetic acid, which is an active component of the wound healing ointment cygerol, have been synthesized. Hydrocarbon chains C10 have been obtained by the one-pot method of formation of long-chain hydrocarbons, namely, by the telomerization reaction of 1,3-dienes with nucleophiles catalyzed by palladium complexes. A heterogeneous palladium-zeolite catalyst has been used for the first time in telomerization of isoprene with piperidine.

The biology of vernix caseosa

The biology and physical properties of the uniquely human skin cream 'vernix caseosa' are discussed. This material coats the foetal skin surface during the last trimester of gestation and provides multiple beneficial functions for the foetus and newborn infant. Vernix has a complex structure similar to stratum corneum but lacks lipid lamellae and is more plastic due to the absence of desmosomal constraints. In utero, vernix is made in part by foetal sebaceous glands, interacts with pulmonary surfactant, detaches into the amniotic fluid, and is swallowed by the foetus. At the time of birth, vernix has a remarkably constant water content approximating 80%. Postnatally, vernix is simultaneously a cleanser, a moisturizer, an anti-infective, and an anti-oxidant. Vernix facilitates acid mantle development and supports normal bacterial colonization. Its hydrated cellular structure and unusual lipid composition provide a 'best' solution for the needs of the foetus and newborn, not least of which is the attraction of caregivers. Vernix is an important natural biomaterial of potential interest to cosmetic scientists, and other disciplines involved in product development and therapies targeting the complex interface between the stratum corneum and a changing terrestrial environment.

The Clinical Evaluation of Pterocarpus santalinus Linn. Ointment on Lower Extremity Wounds—A Preliminary Report

Pterocarpus santalinus is described in the Ayurveda for its wide spectrum of medicinal properties including wound healing. Previously the authors reported animal studies that demonstrated that an ointment made from the bark of this tree was effective without any toxic effects. They used the same ointment in 6 cases of lower extremity wounds. Healing was observed in all wounds. The study was not controlled, the findings are presented here as case studies. Further studies are planned to develop a wound healing ointment from a locally available and inexpensive plant.

Synergistic effect of bismuth subgallate and borneol, the major components of Sulbogin ®, on the healing of skin wound

Most skin lesions heal delay and even heal efficiently within 1–2 weeks, the healed tissue is neither aesthetically nor functionally perfect. Therefore, facilitating skin healing rate and controlling healed skin quality are major aims of drug treatment for a ®wound event. Bismuth subgallate (BS) and Borneol (BO) are the two components of Sulbogin ®, a new Vaseline-based wound healing ointment , one for treating skin wounds. Although BO has antibiotic function, while BS is widely used clinically, neither has been used specifically for wound healing. The experiment described here aimed to study the effect of BS and BO on the healing of skin wounds. This study also compared the effects of BS and BO with Flamazine ® cream, which is currently the most popular drug for wound healing in hospitals. Full-thickness wounds (3 cm×3 cm×0.2 cm) were created on the back of adult male Sprague–Dawley rats. BS, BO, BS+BO, and Flamazine ® were then evenly applied to cheesecloth and placed over the lesion areas. The drug patches were replaced every 2–3 days until the wound areas were completely covered by epidermis in any kinds of drug treatment. The combined BS and BO treatment had the best effect on healing by decreasing lesion area, while increasing granulation tissue formation, re-epithelialization, eating behavior and reconstitution of skin appendages. This investigation showed that BS and BO have a synergistic effect on the skin wound restoration.

Bacteriological and toxicity studies on a wound-healing ointment

Bacteriological and toxicity studies on a wound-healing ointment