Winding Faults Research Articles

Interpolation Pedicle Flap Technique in Wound Defect with Tibial Bone Exposed: A Case Report

Interpolation Pedicle Flap Technique in Wound Defect with Tibial Bone Exposed: A Case Report

Advanced repair of recurrent and low-large hysterotomy defects using a myometrial glide flap

Background The resolution of factors linked to the recurrence of cesarean section defects can be accomplished through a comprehensive technique that effectively addresses the dehiscent area, eliminates associated intraluminal fibrosis, and establishes a vascularized anterior wall by creating a sliding myometrial flap. Objective Propose a comprehensive surgical repair for recurrent and large low hysterotomy defects in women seeking pregnancy or recurrent spotting. Study design A retrospective cohort analysis included 54 patients aged 25–41 with recurrent large cesarean scar defects treated at Otamendi, CEMIC, and Valle de Lili hospitals. Comprehensive surgical repair was performed by suprapubic laparotomy, involving a wide opening of the vesicouterine space, removal of the dehiscent cesarean scar and all intrauterine abnormal fibrous tissues, using a glide myometrial flap, and intramyometrial injection of autologous platelet-rich plasma. Qualitative variables were determined, and descriptive statistics were employed to analyze the data in absolute frequencies or percentages. The data obtained were processed using the InfostatTM statistic program. Results Following the repair, all women experienced normal menstrual cycles and demonstrated an adequate lower uterine segment thickness, with no evidence of healing defects. All patients experienced early ambulation and were discharged within 24 h. Uterine hemostasis was achieved at specific points, minimizing the use of electrocautery. The standard duration of the procedure was 60 min (skin-to-skin), and the average bleeding was 80–100 ml. No perioperative complications were recorded. A control T2-weighted MRI was performed six months after surgery. All patients displayed a clean, unobstructed endometrial cavity with a thick anterior wall (Median: 14.98 mm, IQR 13–17). Twelve patients became pregnant again, all delivered by cesarean between 36.1 and 38.0 weeks, with a mean of 37.17 weeks. The thickness of the uterine segment before cesarean ranged between 3 and 7 mm, with a mean of 3.91 mm. No cases of placenta previa, dehiscence, placenta accreta spectrum (PAS), or postpartum hemorrhage were reported. Conclusions The comprehensive repair of recurrent low-large defects offers a holistic solution for addressing recurrent hysterotomy defects. Innovative repair concepts effectively address the wound defect and associated fibrosis, ensuring an appropriate myometrial thickness through a gliding myometrial flap.

Диагностика неисправностей тягового электродвигателя электропоезда ЭД9Т методом спектрального анализа тока в обмотке якоря

The results of the research in the sphere of the efficiency increase of functioning of the traction electric motor of the ED9T electric train have been presented. Smooth and sustainable railway transport operation depends greatly on the reliability of the traction motor of the ED9T electric train and its efficiency of use. The most widespread damages in the armature winding of the traction motor of ED9T electric train are violations of the terms of the armature commuting, inter-winding faults because of the damage of the insulation of coil turns, closure of winding on the case («ground») and double closures on the ground. The authors offered a logarithmical spectral method of diagnostics allowing to determine malfunctions on the results of mathematical spectrogram processing for rapid clarification of the faults of the armature windings of the traction electric motor.

Sequence Component Based Approach to Detect Faults in Induction Motor Stator Winding

Sequence Component Based Approach to Detect Faults in Induction Motor Stator Winding

The use of power information technology in fault diagnosis of electrical equipment

Abstract As the electric power industry rapidly advances, diagnosing faults in electrical equipment has emerged as a critical challenge. In this study, we leverage advancements in power information technology to develop a method for extracting feature volumes, incorporating multiple characteristics to address mixed faults. Our approach begins with the application of a Backpropagation (BP) neural network to extract fault features from electrical equipment. Subsequently, we employ a Bayesian-optimized Correlation Vector Machine (CVM) classifier for precise diagnosis of mixed faults in transformer windings. We evaluated the performance of our BP-RVM model against traditional diagnostic models through real-world electrical fault diagnosis tests. The results demonstrated high diagnostic accuracy, with a maximum of 99.19%, a minimum of 96.41%, and an average accuracy of 97.73%, indicating a significant success rate in diagnosing types of electrical equipment faults. By providing new theoretical support and technical guidance, this study can improve the accuracy and efficiency of electrical equipment fault diagnosis.

Application of advanced signal processing techniques in vibration analysis and fault diagnosis of multi-dimensional anthropomorphic wind turbines

Abstract Wind turbine operating conditions are complex. To ensure the turbine’s safe operation, it is essential to carry out condition monitoring and fault diagnosis of its vibration. In this paper, from the structure of wind turbines, fault types, and fault formation mechanisms, a wind turbine vibration condition monitoring system is established by designing different vibration condition monitoring sensors and combining them with the Internet of Things technology. The discrete Fourier transform is employed to preprocess the time-frequency data before extracting the specific features of the vibration signal by combining the Hilbert-Huang transform after obtaining the wind turbine vibration signal. The SC-TSFN model with spatio-temporal deep fusion is established to realize the fault diagnosis of wind turbines by combining the replaceable null convolution module, BiLSTM module and the self-attention mechanism. It has been found that when the tertiary meshing frequency fluctuates around 506.98 Hz at a fault characteristic frequency of 16.14 Hz, it indicates a fault in the tertiary high-speed shaft gear. The SC-TSFN model has a fault identification time of approximately 52 days before the actual fault downtime, and the model has a 92.05% accuracy rate for wind turbine fault identification. Relying on the signal processing technology to carry out the wind turbine vibration signal analysis and then input it into the fault identification model can realize the accurate identification of the fault state of the unit and provide technical support for the stable operation of wind turbines.

Condition Monitoring and Fault Diagnosis of Permanent Magnet Synchronous Motor Stator Winding Using the Continuous Wavelet Transform and Machine Learning

Abstract Applying the condition monitoring technology to industrial processes can help detect faults in time, minimise their impact and reduce the cost of unplanned downtime. Since the introduction of the Industry 4.0 paradigm, many companies have been investing in the development of such technology for drive systems. Permanent magnet synchronous motors (PMSMs) have recently been used in many industries. Therefore, the issues of condition monitoring of PMSM drives are important. This study proposes and compares diagnostic schemes based on the stator phase currents (SPCSCs) signal for condition monitoring and fault diagnosis of PMSM stator winding faults. The continuous wavelet transform (CWT) is used for the extraction of the symptoms of interturn short circuits in PMSM stator winding. Machine learning algorithms are applied to automate the detection and classification of the faults. The concept for an original and intelligent PMSM stator winding condition monitoring system is proposed.

A-157 - The practice of using Vacuum-Assisted Closure in combination with free skin grafts in the treatment of wound defects after removal of malignant skin neoplasms

A-157 - The practice of using Vacuum-Assisted Closure in combination with free skin grafts in the treatment of wound defects after removal of malignant skin neoplasms

Activation of reparative processes of chronic wounds using photobiomodulation therapy (experimental study).

Aim: The aim of the work was to study the ef f ect of photobiomodulation therapy on the regulation of disorders in the healing of chronic wounds at the remodeling stage using indicators of platelet aggregation activity, reactive oxygen species, platelet-derived growth factor, and interleukin-1β. Materials and Methods: The study included 3 groups of Wistar rats: intact animals and animals of the control and experimental groups, for which chronic wounds were simulated. Rats in the experimental group received photobiomodulation therapy once a day for 5 days. Wound defects of animals in the control group were fictitiously irradiated. The levels of reactive oxygen species, platelet-derived growth factor, and interleukin-1β in the blood serum of animals were studied by enzyme immunoassay. The functional activity of platelets was measured on a computerized platelet aggregation analyzer using the turbidimetric method. Histological studies were carried out. Results: Changes in the expression of the studied indicators were found in the blood serum of animals with chronic wounds when using photobiomodulation therapy: an increase in platelet-derived growth factor concentrations, the levels of reactive oxygen species and interleukin-1β did not have statistically signif i cant differences compared to the corresponding indicators of animals in the control group. There were no significant differences in the indicators of platelet aggregation activity in the control and experimental groups of animals. Conclusions: The findings suggest that photobiomodulation therapy may promote wound healing by increasing platelet-derived growth factor levels. Histological studies have shown that using photobiomodulation therapy helps reduce inflammation and better organization of collagen fibers in animals of the experimental group.

Features of apoptotic and proliferative processes in experimental infected radiation-induced skin ulcer under conditions of photodynamic therapy and the use of platelet-rich plasma.

. Aim: The purpose of the study was to identify the features of apoptotic and proliferative processes in experimental Staphylococcus aureus-infected radiation skin ulcer under conditions of photodynamic therapy and the use of platelet-rich plasma. Materials and Methods: An experimental study was conducted on 95 six-month-old male rats of the WAG population, which were divided into three groups. Group 1 included 25 animals that were simulated a radiation ulcer of the skin in the thigh area with subsequent application to its surface on the 7th day after irradiation with 0.2 ml of a suspension of the Staphylococcus aureus (ATCC 25923) reference strain (0.5 million microbial cells/cm2). Group 2 included 25 animals with Staphylococcus aureus-infected radiation skin ulcer, which were subjected to photodynamic therapy a day after infection. Group 3 included 45 animals with Staphylococcus aureus-infected radiation skin ulcers, which, 1 day after infection, received photodynamic therapy in the first half of the day, and in the second half of the day the periphery of the wound defect was injected with platelet-rich plasma. The material for the study was skin with underlying soft tissues from the area of radiation exposure. Histological, immunohistochemical, morphometric and statistical methods were used. Results: In cases of simultaneous use of photodynamic therapy and platelet-rich plasma, compared with photodynamic therapy alone, the processes ofapoptosis and proliferation were more balanced, active, with a shift in the proliferative-apoptotic ratio towards proliferation processes and met the needs of the regenerative process. From the 10th to the 22nd day of the experiment these processes increased, which indicated active healing processes, that, during survey microscopy on the 22nd day, were manifested by the complete filling of the wound cavity with granulation and connective tissues with the presence of an epithelial layer on the surface of the regenerate. From the 22nd to the 45th day of the experiment, a decrease in the rate of regeneration was recorded, as evidenced by a decrease in the intensity of apoptotic and proliferative processes. The intensity of the latter was sufficient, which led to the healing of Staphylococcus aureus-infected radiation skin ulcer on the 45th day with complete restoration of the original structure of the skin. Conclusions: Photodynamic therapy in combination with the use of platelet-rich plasma balancedly activates apoptotic and proliferative processes with a predominance of the latter in granulation and connective tissues filling the lumen of Staphylococcus aureus-infected radiation skin ulcer, which on the 45th day of the experiment leads to wound healing with complete restoration of the original structure of the skin.

AgNPs/g-C3N4/PVA antibacterial hydrogel with excellent photocatalytic antibacterial activity for wound dressing

AgNPs/g-C3N4/PVA hydrogel has been prepared by cyclic freeze-thaw method. E. coli was used as the model microbe and full-thickness wound defect models in mice were established to assess the antimicrobial activity of the hydrogel in vitro and in vivo.

Effects of Laurus nobilis Leaf Extract on Healing of Experimentally Induced Wounds in Rabbits.

Wound or injury can be defined as partial or complete separation of the skin, while the healing of the wounds is defined as the sequences of processes by which the skin heals and returns to its normal appearance. This study aims to evaluate the efficacy of Laurus nobilis leaf extracts on the healing of open wounds in rabbits. Twenty-four healthy rabbits were used, divided randomly into control and treated groups, each consisting of 12 rabbits. The rabbits were caused to experience circular wound defects (2.5 cm) in diameter. All animals in the control (C) group were left without treatment while those in the Laurus nobilis leaf extract group were treated with drops of the aqueous extract of Laurus nobilis in a dose of 200 mg/kg per day for 20 days. The results of macroscopic observation revealed that all animals in both groups showed equitable viability and good appetite, no mortality occurred, and no signs of infection. In the group with the Laurus nobilis extract, we noticed a significant improvement in wound-healing activity and a reduction in the wound area at p ≤ 0.05 compared with the C group. Histopathological results at the end of the study show that the thick epidermal layer covers a mass of granulation with congested blood vessels and the dermis transforms into a denser layer gradually due to the improvements of the cellularity in the C group and also revealed a well-formed skin appearance, widespread of collagen, and fibrosis within the dermis with an appearance near the normal dermis in the group treated with Laurus nobilis leaf extract. In conclusion, the results confirmed that using Laurus nobilis methanolic extract solution in a daily dose of 200 mg/kg promotes healing of open wounds in rabbits.

A TA/Cu2+ Nanoparticle Enhanced Carboxymethyl Chitosan-Based Hydrogel Dressing with Antioxidant Properties and Promoting Wound Healing.

As the first line of immune defense and the largest organ of body, skin is vulnerable to damage caused by surgery, burns, collisions and other factors. Wound healing in the skin is a long and complex physiological process that is influenced by a number of different factors. Proper wound care can greatly improve the speed of wound healing and reduce the generation of scars. However, traditional wound dressings (bandages, gauze, etc.) often used in clinical practice have a single function, lack of active ingredients and are limited in use. Hydrogels with three-dimensional network structure are a potential biomedical material because of their physical and chemical environment similar to extracellular matrix. In particular, hydrogel dressings with low price, good biocompatibility, degradability, antibacterial and angiogenic activity are favored by the public. Here, a carboxymethyl chitosan-based hydrogel dressing (CMCS-TA/Cu2+) reinforced by copper ion crosslinked tannic acid (TA/Cu2+) nanoparticles was developed. This study investigated the physical and chemical characteristics, cytotoxicity, and angiogenesis of TA/Cu2+ nanoparticles and CMCS-TA/Cu2+ hydrogels. Furthermore, a full-thickness skin defect wound model was employed to assess the in vivo wound healing capacity of hydrogel dressings. The introduction of TA/Cu2+ nanoparticles not only could increase the mechanical properties of the hydrogel but also continuously releases copper ions to promote cell migration (the cell migration could reach 92% at 48 h) and tubule formation, remove free radicals and promote wound healing (repair rate could reach 90% at 9 days). Experiments have proved that CMCS-TA/Cu2+ hydrogel has good cytocompatibility, antioxidant and wound healing ability, providing an advantageous solution for skin repair.

What are optimum cycles for immediate primary closure of large cutaneous defects?

In the reconstruction of large complex cutaneous wounds, a myriad of mechanical devices has been designed to facilitate primary wound closure. However, there is a dearth of studies elucidating how best to achieve optimum use and efficiency of skin stretching (SS) when using the device for immediate primary closure of defects. Skin defect wounds (7 × 7 cm) were prepared on the back of three Bama miniature pigs. A total of 15 cycles of SS (cycle loading) were subsequently performed on the skin edges of the wound by EASApprox® SS system. Then, the changes in equidistant points were recorded after each cycle. After the SS test, all wounds were sutured under low tension. Skin elongation was observed at all equidistant points on the back wounds of three Bama miniature pigs. Up to an additional 1.10 to 3.75 cm of tissue was garnered. The maximum skin elongation was typically achieved within eight cycles of stretching and relaxation. Beyond this range, additional stretching cycles did not result in further skin extension. There may be a close link between mobilization range and the times of acute cyclic stretching (cycle loading) during the process of primary wound closure. However, larger studies are required to further evaluate the accuracy and effectiveness.

A tilapia skin-derived gelatin hydrogel combined with the adipose-derived stromal vascular fraction for full-thickness wound healing.

Biomaterials are widely used in regenerative medicine to repair full-thickness skin defect wounds. The adipose-derived stromal vascular fraction (SVF) shows pro-regenerative properties, however, the ex vivo biological activity of SVF is suppressed due to the lack of an external scaffold. Tilapia skin, as a sustained and recyclable biomaterial with low immunogenicity, was applied in the preparation of a hydrogel. The mixture of tilapia skin-derived gelatin and methacrylic anhydride as a scaffold facilitated the paracrine function of SVF and exerted a synergistic effect with SVF to promote wound healing. In this study, 30% (w/v) SVF was added to methacrylate-functionalized tilapia skin gelatin and subsequently exposed to UV irradiation to form a three-dimensional nano-scaffolding composite hydrogel (FG-SVF-3). The effects of paracrine growth factors, neovascularization, and collagen production on wound healing were extensively discussed. FG-SVF-3 displayed a pronounced wound healing ability via in vivo wound models. The FG-SVF-3 hydrogel enhanced the biocompatibility and the expression of EGF, bFGF, and VEGF. FG-SVF-3, as a promising wound dressing, exhibited superior ability to accelerate wound healing, skin regeneration, and wound closure.

RESTORE VISION: Novel advanced and repurposed therapeutics for vision restoration in a group of severe rare ocular diseases – Short introductory talk

Rare Eye Diseases (REDs) collectively represent a major cause of visual impairment and blindness for children and young adults in Europe. But importantly, REDs also affect adults and the aging population. Defective wound healing at the cornea and ocular surface, excessive inflammation, nerve degeneration, stem cell dysfunction, and aberrant vessel ingrowth are the common denominators in many REDs, representing a critical medical problem and an area of unmet medical need. The onset and progression of many REDs are characterized by common pathophysiologic mechanisms. The goal of many research groups is to disrupt these aberrant mechanisms with the aim of making therapy more effective. Current management is often prohibitively expensive, has low efficacy and leads to debilitating side effects, pointing to a critical medical problem and area of unmet medical need.Here, we will briefly introduce the RESTORE VISION project, which has been recently funded by the European Commission and brings together actors from across the full value chain: 6 leading research institutions, 3 SMEs and a European patient organization. The project will take a ground‐breaking approach to improve eye health by verifying disease pathomechanisms, using cutting‐edge models for each rare disease to test novel and repurposed compounds and determine drug mechanisms of action, formulating compounds as safe eye drop suspensions or subconjunctival drugs, and performing several first‐in‐human trials of novel therapies.

The use of intralesional epidermal growth factor in the treatment of diabetic foot ulcers

Aims: The mitogenic and cell protective effects of epidemal growth factor (EGF) in wound healing stimulate the growth, re-covering the surface of the wound area. In this research we tried to elucidate the effectiveness of intradermal EGF application on wound healing in diabetic foot ulcers regarding the fact that EGF can accelerate the formation of skin cover layer on the infected surface, even in relatively ischemic cases.
 Methods: The data of 68 patients who applied to our institution’s orthopedics and wound care outpatient clinic with the diagnosis of diabetic foot ulcer, who underwent wound care, debridement and follow-up were retrospectively analyzed. All of the patients included in this study were classified as Wagner Stage III and Stage IV diabetic foot ulcers and were followed up with standard wound care. EGF application was initiated if there was not enough bleeding on the wound borders and defect floor after debridement.
 Results: The rate of patients with 50% or more granulation in the second week of treatment in the groups was 35.7% (n=10) in the standard treatment group, it was 60% (n=24) in the EGF group (p

THE PARTICULARITY PLASTIC CLOSURE OF WOUND DEFECTS IN PATIENTS WITH CHRONIC DIABETIC WOUNDS

Background. Chronic diabetic foot wounds and ulcers are serious complications of the course of diabetes mellitus (DM), which account for 85% of purulent-necrotic lesions of the lower extremities. Pathogenetic mechanisms of "chronization" of wounds in the form of hyperglycemia, neuropathy, and existing infection lead to metabolic disorders in the form of tissue hypoxia and activation of matrix metalloproteinase (MMP) proteolytic enzymes.
 Aim: to study the role of autologous plasminogen on healing processes in diabetic wounds by modulating MMP activity.
 Materials and methods. 45 patients with chronic diabetic wounds, who were divided into 2 groups, participated in the study. Patients of the control group (n=25) were treated according to standard methods. Autologous plasminogen applications were used for treatment in 20 patients of the main group.
 Results. On the 18th day of treatment, a 3.5-fold decrease in the activity of MMP-2,-9 and complete healing of wounds was noted in 16 patients. In 4 patients, the wound defects were closed by the method of autodermoplasty with complete engraftment of grafts. In the patients of the control group, the level of MMP activity remained stably high throughout the observation period.
 Conclusions. The use of applications of autologous plasminogen allows to modulate the activity of MMP and create favorable conditions for the healing of chronic diabetic wounds.

Biomimetic amniotic/silicone-based bilayer membrane for corneal tissue engineering

Amniotic membrane (AM) is an effective and widely used dressing in ocular injuries to reconstruct the cornea. Due to its low mechanical strength, high biodegradation rate, and difficult handling, its usage in medical interventions remains challenging. In this study, decellularized AM was covered with an ultrathin layer of Polydimethylsiloxane (PDMS) through a spinning method, which in turn resulted in an ultrathin (less than 80 µm in thickness) bilayer corneal wound dressing membrane with improved mechanical behavior and transparency. The biomechanical, biological, and antibacterial properties of the bilayer membranes were measured both in vitro and in vivo. The optimized microsized membrane was applied on a corneal defect wound created in a rabbit model to evaluate the corneal healing. The results demonstrated a significant decrease in degradation rate, improved mechanical properties, and AM/PDMS transparency compared with AM.Thecorneal transparency improved until 21 days post-surgery inAM/PDMS group. Histological evaluations revealed that AM/PDMS had better epithelial delaminated cell morphology. The results of the RT-PCR showed a significant increase in MMP9, a significant decrease in Col1A1, TGF-β1, TNF-α and IL-6 in both AM and AM/PDMS compared with control wounds. This study suggessts AM/PDMS membrane as an excellent corneal wound dressing.

Simulation of a Trophic Purulent Wound: an Experimental Study

Background. Long-term non-healing wounds are one of the common complications of multiple diseases, injuries and surgical interventions. In order to optimize their treatment options, experimental simulation of the wound process are created and improved.The aim of study was to develop an experimentally simulated trophic purulent wound, and to evaluate the potential of its use to study the impact of various factors on the wound process.Materials and methods. The trophic purulent wound simulation was performed on 80 nonlinear albino rats. Experimental wounds were formed using a silicone disk, the inner edges of which were sutured to a round wound formed in the interscapular region of the animal. Next, the silicone disc was sutured to the skin along the outer edge and additional sutures were placed around the disc to enhance tissue ischemia. The fascia at the bottom of the wound was incised, the bottom of the wound was crushed with a clamp, and a bacterial culture was injected into the wound defect. The developed model was evaluated visually, using thermometry, luminescent analysis, planimetry, microbiological, cytological and morphological tests.Results. The features of the simulated wound allowed achieving the size and protection similar to experimental wounds, and forming trophic disorders in the tissues, In 48 hours, a picture of a complicated purulent process was observed in most animals. The data obtained during the analysis of the proposed experimental model confirmed its quality, simplicity and reproducibility.Conclusion. The proposed approach can be recommended to study protracted wound processes and various factors affecting them.