The aim of the study was to verify the current reports on the relationship between shift work and situational factors with positive and negative well-being emotional components of P Warr’s theory. The IWP Multi-Affect Indicator scale by Warr (1990) in the Polish adaptation of E. Mielniczuk and M. Łaguna, as well as questions of situational factors related to the work: workload of the current day, occurrence of negative critical situations on a given day, difficulty of the tasks performed on that day, evaluation of current working conditions. The sample consisted of 50 shift workers representing different professions, with women’s advantage. Most of them are young people with short work experience, coming from a large city. The measurement was carried out five times per shift: in the morning and in the afternoon. The research was conducted in an individual form. The results of the research indicate that work in the afternoon shift is associated with poorer well-being (H1), work in the morning shift is associated with better employee well-being (H2), situational factors such as workload, difficulty of employee tasks, negative critical events and bad working conditions generate negative well-being components, regardless of the shift the employee works on (H3), and that the shift will have an impact on perceived well-being regardless of the impact of the above mentioned situational factors (H4). The results of the research can be applied in the practice of organizational management and may constitute further research inspirations. Key words: well-being at work, emotions at work, work shift, Warr’s theory, situational factors