Objective: To understand the influencing factors of job burnout among nurses in Haikou 3A hospital and explore its direct and indirect effects, so as to provide a scientific basis for the work efficiency of nursing staff. Methods: Between November 2, 2015 and November 2015, using multi stage random sampling, self-administered questionnaire survey was conducted among 1049 nursing staff, using the path analysis method to study the effect of direct and indirect factors effect. Results: The total score of job burnout of nurses was 38.44 ± 7.55, high occupational burnout was 0.9%, moderate occupational burnout was 66.5%, and low occupational burnout was 32.6%. The scores of job burnout were compared among the nurses with different titles, and less achievement (F = 8.342, P 5 dimensions of work pressure source (0.125) > title (−0.112) job satisfaction scores (−0.097). Conclusion: Job stress, job satisfaction and job title are the factors that affect job burnout. The 4 and the direct and indirect effects of job stressors are the strongest, and measures should be taken to solve these problems.