Male Wistar Strain Rats Research Articles

Reperfusion Interval as a Prevention of Lung Injury Due to Limb Ischemia-Reperfusion After Application of Tourniquet in Murine Experimental Study.

Tourniquet use is prevalent in the orthopaedic field to achieve a bloodless operating field, but it poses risks of local and systemic complications, including lung injury. This study aims to examine the effect of tourniquet application on the hindlimb of a rat to its lung. This is an experimental study with 48 male Wistar strain rats as samples. The rats were divided into group A (n = 24), killed directly after fracturization and tourniquet application, and group B (n = 24), killed 14days post-procedure. Each group was divided into four: group A1/B1 (control group, three hours tourniquet application without reperfusion interval), A2/B2 (5-min reperfusion between 2-h and 1-htourniquet application), A3/B3 (10-min reperfusion), and A4/B4 (15-min reperfusion). The lung tissue was examined histologically within ten high-power fields (400 × magnification). The severity of lung injury was measured using the Lung Injury Score (LIS). The oxidative damage was measured by determining the malondialdehyde (MDA) level, using the TBARS (thiobarbituric acid reactive substance assay) method. There was a dose-dependent decrease of LIS and MDA in groups A and B with increasing reperfusion interval. Fifteen-minute reperfusion interval caused a 54.55% and 45.33% LIS reduction in groups A and B, respectively. All pair-wise group comparisons (p < 0.05) showed significant differences. Five-minute interval reduced the MDA level by 16.56% and 30.13% in groups A and B, respectively. All possible pair-wise comparisons in both groups A and B also showed a significant difference (p < 0.05). Reperfusion interval is a possible clinical approach to mitigate the remote organ damage induced by limb ischemia-reperfusion injury.

Effect of various inductions of sleep deprivation stress on proinflammatory cytokine levels in gingival crevicular fluids of white male Wistar strain rats (Rattus novergicus)

Background: Stress that is induced by sleep deprivation can modulate the damage of periodontal tissue by elevating the levels of proinflammatory cytokines (i.e. IL-1β and TNF-α). The effects of sleep deprivation can be resolved with sleep recovery. Gingival crevicular fluid (GCF) is fluid in sulcular gingiva which acts as an oral biomarker for evaluating periodontal abnormalities. Purpose: The aim of this study was to determine the effect of various induction methods of sleep deprivation stress on cytokine levels in GCF of white male Wistar strain rats (Rattus novergicus). Methods: The study method was true experimental with a posttest-only control group design. Thirty male Wistar rats were randomly divided into five groups: paradoxical sleep deprivation (PSD), total sleep deprivation (TSD), partial sleep deprivation with sleep recovery for five days (PSD+SR), total sleep deprivation with sleep recovery for five days (TSD+SR) and a healthy control group. Data were analysed via one-way ANOVA to determine differences between groups. Result: The results showed the highest level of IL-1β and TNF-α was found in the PSD group. One-way ANOVA analysis showed significant differences (p&lt;0,05) of IL-1β level between PSD and control groups, PSD and PSD+SR groups and PSD and TSD+SR groups; in contrast, the analysis of TNF-α levels showed significant differences (p&lt;0,05) between PSD group to control group, PSD to PSD+SR group and TSD to TSD+SR group. Conclusions: There is an effect of various induction methods of sleep deprivation stress on proinflammatory cytokines (IL-1β and TNF-α).

Effect of Tender Coconut Water (TCW) on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) Induced Diabetic Rats

Background: Diabetes Mellitus (DM) is characterized by an increase in blood sugar levels also known as hyperglycemia. Continuous hyperglycemia can increase the production of Reactive Oxygen Species (ROS). ROS causes oxidative stress and increases the formation of TNF-α expression as a marker of inflammation. Tender coconut water is rich in sources of free amino acids, L-arginine and vitamin C which can prevent oxidative stress. Aim and Objectives: This research to investigate the effect of tender coconut water on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) induced diabetic rats. Material and Methods: Experimental research design using posttest control group design. Twenty four male wistar strain rats were used in this study were divided randomly into 4 groups, which are group K1 (control); K2 (DM type 2); K3 (DM type 2+ Glibenclamid 0,18mg/200grBW); K4 (DM type 2+ tender coconut water 8mL/200gr BW). Type 2 Diabetes Mellitus were induced using Streptozotocin (STZ) 65mg/kg body weight and Nicotinamid 230 mg/kg body weight. The administration of tender coconut water were given on day 3 after DM condition is reached, given daily for 4 weeks with dose of 8 mL/200 gr BW. Data on of TNF-α , IL-1 and IL-6 levels were analyzed by One Way Anova. Result: Average TNF-α level, IL-1 level and IL-6 level in Group 2 increased compared to Group 1 , in Group 3 it decreased compared to Group 2 as well as in Group 4 .The results of the analysis has the p values <0.05. Conclusion: Administration of tender coconut water can be decreasing of TNF-α, IL-1 and IL-6 levels in wistar strain male rats with type 2 Diabetes Mellitus.

THE SUB-CHRONIC TOXICITY TEST OF MENIRAN (Phyllanthus niruri L.) AND PEGAGAN (Centella asiatica) EXTRACT IN WISTAR STRAIN RATS ON LIVER AND KIDNEY FUNCTION

Meniran (Phyllantus niruri L.) and Pegagan (Centella asiatica L.) was proven had activity that considered as an antioxidant. However, the safety was not confirmed. This research is intended to evaluate sub-chronic toxicity of standardized combination extract of meniran and pegagan on liver and kidney function. This research used post-test-control design. Twenty female and twenty male Wistar strain rats divided into 4 groups. The first group was controlled in dosage of CMC Na 0.5%. The others were given a combination of meniran and pegagan extract in ratio with different dosage (50:50; 250:250; and 1250:1250 mg/KgBW). Sub-chronic toxicity test of meniran and pegagan combination was given orally once a day for 28 consecutive days. On the 29th day, all the rats were sacrificed and blood samples were analyzed using automatic analyzer. SGOT, SGPT, BUN, and creatinine value were statistically analyzed using one way ANOVA with post hoc LSD (p&lt;0.05). The results show that treatment of meniran and gotu kola combination had no significantly different of SGOT and creatinine value of male and female rats, and SGPT value of male rats (p&gt;0.05). The treatment of meniran and gotu kola combination had significantly different of BUN value of male and female rats, and SGPT value of female rats (p&lt;0.05) but still within normal range. Based on this study, it can be concluded that orally administered of meniran and gotu kola extract combination at doses 50:50 mg/KgBW; 250:250 mg/KgBW; and 1250:1250 mg/KgBW had no affected on SGOT, SGPT, BUN and creatinine value of Wistar strain rats on sub-chronic administration for 28 consecutive days.

Evaluation of Mutagenic and Antimutagenic Activity of Methanol Extract of Cousinia thomsonii against Cyclophosphamide

The present study was conducted to evaluate the mutagenic and antimutagenic potential of Cousinia thomsonii (CT) extract in bone marrow cells of male wistar rats using some important parameters like micronucleated polychromatic erythrocyte (MnPCE), mitotic index (MI), chromosomal aberrations (CA) and polychromatic erythrocyte to normochromatic erythrocyte ratio (PCE/NCE). 30 male rats of wistar strain were divided into 6 groups with 5 rats each group. Group 1 rats were taken as negative control having free access to distilled water and rat feed. Group 2 rats were taken as positive control treated with mutagen cyclophosphamide (CP) at dose of 60 mg/kg b wt. for 2 days. Group 3 and 4 were treated with CT extract at dose of 100 and 200 mg/kg b wt. for 20 days. Group 5 and 6 were treated with 100 and 200 mg/kg b wt of CT extract for first 18 days and for last 2 days with CP at dose concentration of 60 mg/kg. It was found that rats treated with CT extract alone did not produce any significant changes in MnPCE, PCE/NCE ratio, CA and MI when compared with control treated rats (group 1). However in group 5 and 6 rats treated with CT extract in combination with CP a protective effect was observed against the cyclophosphamide induced cellular mutagenicity. In concluding remark Cousinia thomsonii was found to show antigenotoxic potential and also produce protective antimutagenic effects against CP induced chromosomal damage.

Pengaruh Ekstrak Buah Mengkudu (Morinda Citrifolia) Terhadap Kadar Kolesterol Total Darah Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

Background: High cholesterol diet induced hypercholesterolemia. Noni fruit extract has flavonoids which are expected to reduce blood total cholesterol levels.&#x0D; This study aims to determine the effect of noni fruit extract (Morinda citrifolia) on total blood cholesterol levels in wistar strain male rats (Rattus norvegicus) given a high-fat diet.&#x0D; Method: Experimental study using post test only control group design. A total of 24 wistar strain male rats were divided into 3 groups: negative control group given standard feed; positive control group of experimental animals who were given a high-fat diet; the experimental group treated with a high-fat diet for 14 days and given noni fruit extract at a dose of 200 mg / Kg BB. On the 29th day total blood cholesterol levels were measured for all groups of experimental animals.&#x0D; Results: The Mann Whitney U Test showed a significant increase in total blood cholesterol levels in the positive group of experimental animals given a high-fat diet (68.13 mg/dl) compared to the negative control group of experimental animals given standard feed (51.25 mg/dl). There was a significant decrease in total blood cholesterol levels in the experimental group treated with a high-fat diet and noni fruit extract (61mg/dl) compared to the average positive control group of experimental animals given a high-fat diet ( 68.13 mg/dl).&#x0D; Conclusion: Provision of a high-fat diet in wistar strain male rats can significantly increase total blood cholesterol levels. The administration of noni fruit extract in wistar strain male rats can significantly reduce total blood cholesterol levels.

Aktivitas Antihiperurisemia Mikroenkapsulasi Ekstrak Kulit Melinjo (Gnetum gnemon L.) secara In Vivo

Hyperuricemicis as an abnormally elevated uric acid level in the blood.Gnetumgnemon, locally known as Melinjo, is one of the Indonesian indigenous plantswhichcan be an alternative source of allopurinol. The peels of Gnetumgnemon, usually be wasted,contains red anthocyanin pigments possessing antioxidant activity owing toits potential for the inactivation of free radicals and peroxyls. However, the anthocyanin will chemically result incompound instability and is easily degraded during the period ofstorage. Encapsulation remains as one of the eminent formulation techniques usedto protect the active pharmaceutical compound through coating ofmainingredient with polymer forming smallersize of particles within micro tonano.This study was aimed at determining the Gnetumgnemonpeel extract in microencapsulated form in inducing antihyperuricemic activity. The male Wistar-strain rats wereused as subjects,groupedinto fivedifferent feeding treatments, i.e. negative group (carboxymethyl cellulose(CMC) Na 0.5%), positive group (allopurinol 12.6 mg/kg BM), andthree doses groups(i.e. 100 mg/kgBM, 200 mg/kg BM, dose group 300 mg/kg BM). Uric acid levels were initially assessed for each group, then every test-animals werefedwith a dose of 300mg/kg BM of potassium oxonate intraperitoneally,and 2.5 mL of chicken liver juiceorally. An hour interval was given for the uric acid levels measurement for each group. The results showed an increase in uric acid levels after the subjectswere treated accordingly with potassium oxonate 300mg/kg BM and 2.5 mL chicken liver juice. At the hour-2, there was a decrease in uric acid levels but showingno significant difference in everygroup (p&gt; 0.05). The significant decrease among the groups was found at hour-4and indicates that dose of 200mg/ kgBM is an effective dose in reducing uric acidlevels Keyword: Gnetumgnemonpeel extract, microencapsulation, antihyperuricemic

The Effect of Irvingia gabonensis Ethanol Extracts (Ogbono) Seeds on the Growth rate and Hematological Parameters in Wistar rats.

Aim: To evaluate the effect of Irvingia gabonensis ethanol extract on the growth-rate and haematological parameters in Wistar rats. Method: This research was a randomized-controlled study of two groups using ten Male Wistar strain rats within the age of two months, weighing 80 g-146 g. Group one as the control and Group two as the test. During the study, distilled water and rat feed was given to the group one, and an oral administration of 250 mg/kg Irvingia gabonensis was given to group two for 14 days using oral gavage. The growth rate of the Wistar rats was measured as its length using a tape rule within 3 days throughout the study period. Finally, blood samples were obtained for haematological analysis using the BC-5380 Auto Haematology Analyzer. Result: We discovered that an oral administration of 250 mg/kg Irvingia gabonensis significantly increased (p<0.05) the Mean Corpuscular Haemoglobin Concentration (34+0.27) g/dl of the test group when compared to the control (33.23+0.23) g/dl. However, the result didn't indicate a significant change in the growth rate of both groups during the 14 days of study. Conclusion: The ethanol extract of Irvingia gabonensis seeds doesn't negatively affect blood parameters. Thus, suggesting the use of Irvingia gabonensis extract for the maintenance of normal blood function.

Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT &amp; SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p &lt;0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p &lt;0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT &amp; SGPT in male wistar strain rats with acute hepatitis model.

Effectiveness of Boiled Cherry Leaf (muntingia calabura L) and Figs Leaves (Ficus Carica) Toward SGOT SGPT Serum of Male Wistar Strain Rats with Acute Hepatitis Models

Hepatitis is inflammation of liver cells. There are two contributing factors: infectious factors and non-infectious factors. Factors causing infection include hepatitis viruses and bacteria. Non-infectious factors for example of drugs usage. The purpose of this study was to determine the effectiveness of boiled cherry leaves (muntingia calabura L) and Figs leaves (Ficus Carica) to reduce SGOT SGPT levels. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were divided into 3 groups randomly: negative control group, positive control group and treatment group. The positive treatment and control group was induced paracetamol 120 mg / day orally for 7 days. 0.4 grams of Figs leaves (Ficus Carica) and 5 grams of Cherry leaves (muntingia calabura L) is boiled in 200 cc of water to 100 cc of water given as much as 3.6cc orally for 7 days in the treatment group. Data were analyzed with SPSS version 24, ANOVA test was performed to compare SGOT SGPT levels. The results showed there were significant differences in SGPT SGPT levels between the treatment and positive control groups (p &lt;0.05). In conclusion, the boiled water of Figs leaves (Ficus Carica) and cherry leaves (muntingia calabura L) have an effect in decreasing SGOT SGPT serum in male wistar strain rats with acute hepatitis model

The Effectiveness of Boiled Rosy Periwinkle (Catharanthus roseus) in Decreasing SGOT and SGPT Value of Male Wistar Strain Rats with Acute Hepatitis Model

Acute hepatitis is a disease that attacks the liver and causes inflammation in the liver cells which is characterized by swelling, damage, death, and even disruption of liver function, so that it can result in increased values of Serum Glutamic Pyruvic Oxaloactic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT). This study aims to determine the effectiveness of the Rosy Periwinkle (Catharanthus roseus) on decreasing SGOT and SGPT level. The object of this research is 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams which were adapted for 7 days. The object of this study was divided into 3 groups, namely the treatment group, the positive control group and the negative control group. The treatment group and positive control group were induced Paracetamol 120 mg / day orally for 7 days. 5.2 grams of boiled Rosy Periwinkle (Catharanthus roseus) as much as 3.6 cc orally for 7 days was given to the treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test to compare SGOT and SGPT values. The results of this study found that there were a significant difference in the SGOT value between the treatment group, the positive control group and the negative control group (p &lt;0.05) and there were significant differences in the SGPT value between the treatment group and the positive control group (p &lt;0.05) . The conclusion of this study is that the boiled Rosy Periwinkle (Catharanthus roseus) has an effect on the decrease in SGOT and SGPT values in male wistar strain Rats with acute hepatitis model.

The Effectiveness of Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia calabura L.) Decoction on SGOT and SGPT Serum in Male Wistar Strain Rats of Acute Hepatitis Model

Acute hepatitis is inflammation that occurs in the liver caused by infection, drugs, autoimmune, or alcohol consumption and causes an increase in SGOT and SGPT levels. This study aims to determine the effectiveness of boiled Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar Strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control, and negative control. All rats were adapted for 7 days. The liver of treatment group and the positive control group were induced Paracetamol 120 mg/day orally for 7 days. Boiled water of 2.6 grams of Rosy Periwinkle (Catharanthus roseus) and 5 grams of Cherry (Muntingia calabura L.) as much as 3.6 cc/day orally for 7 days was given to the treatment group. The Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group and negative control group (p&lt;0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p&lt;0.05). The conclusion of this study is that the boiled water of Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia calabura L.) have an effect on decreasing SGOT and SGPT serum in male Wistar Strain rats of acute hepatitis model.

Effectiveness Rosy Periwinkle (Catharanthus roseus L.) Decoction Toward Urea and Creatinine Serum Levels of Male Wistar Strain Rats with Acute Kidney Failure Model

Acute kidney failure is a disorder that occurs suddenly in the ability of the kidneys toinfiltrate blood, which is characterized by increased levels of urea and creatinine within a fewhours or days. This study aims to determine the effectiveness of the Rosy Periwinkle (Catharanthus roseus L. ) on the levels of urea and creatinine in male Wistar Strain with acutekidney failure models. The object of the study were30 male Wistar Strain Rats aged 2-3months with a weight of 180-200 grams per rat. The study was divided into three groups,namely the treatment group, positive control group, and negative control group. Each groupconsists of 10 male rats. Tread leaves taken as much as 5.2 grams are boiled in 300 cc ofwater until the remaining 100 cc of boiled water. The treatment group and positive controlgroup induced gentamicin 12 mg in 0.3 cc intraperitoneal / rat / day for 7 days. The treatmentgroup was given boiled water for as much as 3.6 cc / day orally for 7 days. Giving agentamicin injection to damage the kidneys. Data collection on urea and creatinine levels wascalculated using the SPSS program version 24 One Way ANOVA test to compare creatinineurea levels. The results showed that there were significant differences in creatinine urealevels between the treatment group and the positive control group (p &lt;0.05). The conclusionof this study is that the boiled water of Rosy Periwinkle ( Catharanthus roseus L. ) is effectivein reducing the level of urea and creatinine of male Wistar Strain Rats with acute kidneyfailure models

Effectiveness of Boiled Rosy Periwinkle (Catharantus roseus), Cherry (Muntingia calabura L.), comfrey (Symphytum officinale), and Figs (Ficus carica) Toward the reduction of SGOT and SGPT serum in male Wistar strain rats with acute hepatitis model

Hepatitis is inflammation of the liver caused by viruses, alcohol and drugs or other pathogens. The purpose of this study was to compare the effectiveness of rosy periwinkle (Catharantus roseus), cherry (Muntingia calabura L.), comfrey (Symphytum officinale), and figs (Ficus carica) toward decreasing serum levels of SGOT and SGPT. The object of this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams which were adapted for 7 days. The rats then divided into 3 groups, namely the positive control group, the negative control group, and the treatment group. After 7 days of the adaptation process, the liver of the treatment group and the positive control group was induced Paracetamol 120 mg / day orally for 7 days. Boiled water therapy of 3.5 grams of Rosy Periwinkle (Catharantus roseus), 3.5 grams of cherry (Muntingia calabura L.), half a sheet of comfrey (Symphytum officinale), and 0.3 gram of figs (Ficus carica) was given to the treatment group during the treatment group in 7 days as much as 3.6 cc / orally per day. Data were analyzed using SPSS version 24, One Way ANOVA test to compare SGOT SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p &lt;0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p &lt;0.05) . The conclusion of this study is the mixed boiled water of Rosy Periwinkle (Catharantus roseus), Cherry (Muntingia calabura L.), comfrey (Symphytum officinale), and Figs (Ficus carica) have an effect on decreasing SGOT SGPT serum in male wistar rats with acute hepatitis model.

Effectiveness of Boiled Cherry Leaf (muntingia calabura L) Toward Ureum Creatinine Serum of Wistar Strain Mice with Acute RenalFailure Model

Renal failure is a clinical condition that is characterized by a decrease in kidney function with an indicator of changes in the levels of urea and creatinine. The purpose of this study is to determine the effectiveness of Boiled Cherry leaf (muntingia calabura L) on the reduction of ureum and creatinine levels. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly grouped into 3 groups: the treatment group, positive control and negative control. The positive treatment and control group was induced gentamicin 0.3 cc IP / day for 7 days. Boiled water from 10 grams of cherry leaves (muntingia calabura) in 200 cc of boiling water to 100 cc was given as much as 3.6 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, One test ANOVA was performed to compare urea and creatinine levels. The results showed that there were significant differences in urea and creatinine levels between the treatment group and the positive control group (p &lt;0.05). Urea and creatinine values between the treatment group and the negative control group were not different (p = 0.06). As Conclusion , boiled cherry leaves (muntingia calabura) has an effect on the reduction of urea and creatinine serum in male wistar strain rats with acute renal failure models.

The Effectiveness of Figs Leaf (Ficus Carica L) and Rosy Periwinkle (Catharantus roseus) Decoction on SGOT and SGPT Levels of Male Wistar Strain Rats with Hepatitis Model

Hepatitis is an inflammatory disease of liver cells that is contagious in the low category, the cause of it is bacteria, viruses, drugs and alcohol. The purpose of this study was to determine the effectiveness of boiled figs leaf (Ficus Carica L) and Rosy periwinkle (Catharantus roseus ) in reducing SGOT and SGPT levels. The subjects of this study were 30 male Wistar strain rats with age of 2-3 months, body weight 180-200 grams. Rats were divided into 3 groups; treatment groups, negative control groups and positive control groups. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. The treatment group was given 0.4 grams boiled figs leaf (Ficus Carica L) and 2.6 grams boiled rosy periwinkle (Catharantus roseus) it given as much as 3.6 cc / day orally for 7 days. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare SGOT and SGPT levels. The results showed there were significant differences in SGOT levels between the treatment group, the positive control group, and the negative control group (p &lt;0.05) and there was a significant difference in the SGPT value between the treatment group and the positive control group (p &lt;0.05), while the negative control group and the treatment group there was no significant difference (p = 0.75). The conclusion of this study was the water extraction of figs leaf (Ficus Carica L), Rosy periwinkle (Catharantus roseus) had an effect in decreasing the SGOT and SGPT values of male wistar strain rats induced by paracetamol.

Effectiveness of Figs (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia Calabura L.) Decoction on Decreasing SGOT and SGPT Serum in Male Wistar Strain Ratswith Acute Hepatitis Model

Acute hepatitis is inflammation of the liver. This disorder is usually caused by a virus or exposure to alcohol, drugs, toxic, or other pathogens. The purpose of this study was to determine the effectiveness of boiled figs leaves (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus), and cherry (Muntingia Calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 0.3 grams Figs (Ficus carica L.), 3.5 grams of rosy Periwinkle (Catharanthus roseus), and 3.5 grams of cherry (Muntingia Calabura L.) was given as much as 3.6 cc orally for 7 days to treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT and SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p &lt;0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p &lt;0.05). The conclusions of this study were mixed boiled of Figs (Ficus carica L.), rosy Periwinkle (Catharanthus roseus), and and Cherry (Muntingia Calabura L.) had an effect in decreasing serum SGOT and SGPT in male rats with acute hepatitis model.

Effectiveness of Boiled Figs Leaf (Ficus Carica L) Towards Ureum and Creatinin Serum of Male Wistar Strain Rats with Acute Renal Failure Model

Acute renal failure is a set of clinical symptoms characterized by increased levels ofcreatinine and blood urea nitrogen and a rapids decrease in the rate of glomerular filtration.The Objectives of this study was to determine the effectiveness of brewed tin water (FicusCarica L) against urea and creatinine serum of male Wistar strain rats in acute renal Failure.The subject of the research were 30 male rats weighing 180-200 grams and 2-3 months old,which were divided into 3 groups, namely the negative control group, the positive controlgroup and the treatment group. Each group consisted of 10 rats. The negative control groupwas only given food and drink as usual. The positive control group and the treatment groupwere induced with gentamicin 12 mg / intraperitonial for 7 days to increase urea andcreatinine serum levels. The treatment group was given steeping treatment of 0.65 grams ofboiled figs leaf in 100cc of given 3.6cc/orally for 7 days. One way ANOVA test wasconducted to analyse the study using SPSS version 24. The results found there are nosignificant differences in urea serum levels between the treatment group and negative controlgroup (p = 0.252) but there were significant differences in the urea serum value between thetreatment group and positive control group (p &lt;0.05). There was a significant difference inthe value of creatinine serum between the treatment group, the positive control group and thenegative control group (p &lt;0.05). As Conclusions boiled figs leaf (Ficus Carica L) has aneffect on decreasing urea and creatinine serum levels in male wistar rats with acute renalfailure.

Effectiveness of boiled Comfrey Leaf (Symphytum Officinale L) on Ureum Creatinine Serum in Male Wistar Strain of Acute Kidney Failure model.

Acute kidney failure is a decrease in kidney that occurs only in a matter of hours even days which can be seen from decreased urine production and increased blood urea nitrogen and creatinine. This study aims to determine the effectiveness of boiled comfrey leaf (symphytum officinale L) on Ureum Creatinine Serum in Male Wistar Strain of Acute Kidney Failure model. This research used 30 male Wistar strain rats weighing 180-200 grams and aged 2-3 months that were divided into 3 groups, namely negative group (only given minerals), positive group (kidney damaged by gentamicin 0.3cc IP / day) and the treatment group (the kidneys were damaged with gentamicin 0.3cc IP / day and 2.8 g of boiled comfrey leaf in 150 cc of water and 2.7 cc were given orally for 7 days). Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare the levels of ureum and creatinine. The results showed that there was a significant difference in the ureum of the treatment group and the positive group (p &lt;0.05) but there was no significant difference in the ureum value between the treatment group and the negative group. There was a significant difference in creatinine values between the treatment group, positive group and negative group (p &lt;0.05). As a Conclusion boiled comfrey leaf (symphytum officinale L) water has an effect on decreasing serum levels of ureum creatinine in male rats wistar strain of acute kidney failure model.

Targeting Wistar rat as a model for studying benign, premalignant and malignant lesions of the prostate

AimsThe purpose of this study was to describe a suitable experimental model for studying aging-related prostate disorders including cancer. Materials and methods12-month old Wistar rats were kept in control conditions (n = 12) or treated (n = 16) for 6 months with Silastic implants filled with testosterone (T) and estradiol (E2). After the experiment period (at 18 months of age), animals were euthanized and the prostate and other organs were harvested, dissected, weighed, and processed for morphological, ultrastructural and molecular analyses. Key findingsWe demonstrated that male rats of Wistar strain nicely recapitulate the carcinogenesis process taking place in the aging prostate through the arising of benign, precancerous and malignant lesions, and above all yields a modest incidence of spontaneous PCa (~36%). Moreover, our results highlight that 100% incidence of PCa and precancerous lesions such as prostatic intraepithelial neoplasia and proliferative inflammatory atrophy were achieved in this rat strain after T + E2 treatment, without changing the broad spectrum of changes that naturally emerge in the prostate at advanced ages. Such enhancement of precancerous lesions and tumors was linked to a decreased expression of E-cadherin and β-catenin in parallel with an increase in Vimentin and N-cadherin, hallmark modifications of epithelial-mesenchymal transition. SignificanceOur findings provide solid evidence that aged Wistar rats may be an excellent model for studies regarding human prostate biology and related disorders including cancer.