Winter Wheat Cultivars Research Articles

Effects of genetic components of plant development on yield-related traits in wheat (Triticum aestivum L.) under stress-free conditions.

The dynamics of plant development not only has an impact on ecological adaptation but also contributes to the realization of genetically determined yield potentials in various environments. Dissecting the genetic determinants of plant development becomes urgent due to the global climate change, which can seriously affect and even disrupt the locally adapted developmental patterns. In order to determine the role plant developmental loci played in local adaptation and yield formation, a panel of 188 winter and facultative wheat cultivars from diverse geographic locations were characterized with the 15K Illumina Single Nucleotide Polymorphism (SNP) chip and functional markers of several plant developmental genes and included into a multiseason field experiment. Genome-wide association analyses were conducted on five consecutive developmental phases spanning from the first node appearance to full heading together with various grain yield-related parameters. The panel was balanced for the PPD-D1 photoperiod response gene, which facilitated the analyses in the two subsets of photoperiod-insensitive and -sensitive genotypes in addition to the complete panel. PPD-D1 was the single highest source, explaining 12.1%-19.0% of the phenotypic variation in the successive developmental phases. In addition, 21 minor developmental loci were identified, each one explaining only small portions of the variance, but, together, their effects amounted to 16.6%-50.6% of phenotypic variance. Eight loci (2A_27, 2A_727, 4A_570, 5B_315, 5B_520, 6A_26, 7A_1-(VRN-A3), and 7B_732) were independent of PPD-D1. Seven loci were only detectable in the PPD-D1-insensitive genetic background (1A_539, 1B_487, 2D_649, 4A_9, 5A_584-(VRN-A1), 5B_571-(VRN-B1), and 7B_3-(VRN-B3)), and six loci were only detectable in the sensitive background, specifically 2A_740, 2D_25, 3A_579, 3B_414, 7A_218, 7A_689, and 7B_538. The combination of PPD-D1 insensitivity and sensitivity with the extremities of early or late alleles in the corresponding minor developmental loci resulted in significantly altered and distinct plant developmental patterns with detectable outcomes on some yield-related traits. This study examines the possible significance of the above results in ecological adaptation.

Early–maturing cultivar of winter wheat is more adaptable to elevated [CO2] and rising temperature in the eastern Loess Plateau

Understanding the impacts of rising temperature and elevated [CO2] on different crop cultivars is critical to devising agronomic strategies and cultivar selection in the context of climate change adaptation. The growth period, biomass, and yield of two wheat cultivars (early– vs. late–maturing) were simulated by the Agricultural Production Systems sIMulator (APSIM) validated with pot experiments under the scenarios of elevated [CO2] and 2 °C warming based on the baseline climate for 1961–2016 at Taigu in North China. We also investigated the effects of extreme high temperature on the yield and yield components of these wheat cultivars. We found that APSIM well simulated the changes in the growth period, leaf area index (LAI), biomass, and yield induced by 2 °C warming and elevated [CO2]. Long–term simulations showed that 2 °C warming decreased the growing period between sowing and jointing for both cultivars. 2 °C warming and elevated [CO2] affected the wheat yield mainly by altering the grain numbers. Extreme high temperature decreased the grain numbers and yield of the late–maturing cultivar but not the early–maturing one. The findings suggest that early–maturing cultivar would be better adapted to elevated [CO2] and rising temperature than the late–maturing one in the eastern Loess Plateau.

Evaluation of the impact of photoperiod and light intensity on decreasing days to maturity in winter wheat

AbstractThe development and improvement of crop cultivars are necessary to meet the demands of the growing global population. The ability to decrease the generation time is imperative for breeders to achieve higher rates of genetic gain annually. Understanding the way in which photoperiod affects the maturation rate of these crops, including winter wheat (Triticum aestivum L.), is required for decreasing generation time in breeding programs. The effects of prolonged photoperiod and light intensity on winter wheat generation time were studied to determine if photoperiod may contribute to a shortened cycle time. Three winter wheat cultivars (Branson, CFBA1401, and Norstar) and one spring wheat cultivar (AC Carberry) were exposed to four treatments based on the daily light integral (DLI): high light (HL) or low light and photoperiod as follows: 22 h of light and 2 h of dark (22:2) or 16 h of light and 8 h of dark (16:8). Generation time, as measured by days to maturity in the HL22:2 treatment, resulted in a decrease of 8 days or 7% of the total generation time, compared to the control, HL16:8 treatment. Photoperiod itself had a minimal effect on generation time when both DLI and temperature were controlled. An improved understanding of the effects of photoperiod, thermal time, and DLI and their interactions is required prior to implementation in a winter cereal breeding program.

Nebraska winter wheat unexpected flowering in Egypt: New improvement opportunities

AbstractThe University of Nebraska has bred some of the most winter hardy wheat (Triticum aestivum L.) cultivars in the United States. With the advent of a new race of stem rust in Africa, the breeding program identified a testing site in northern Egypt, a Mediterranean environment, for seedling disease resistance screening. Quite surprisingly, the Nebraska winter wheat lines flowered when grown in northern Egypt, indicating the lines were vernalized. To confirm this result, a panel of 112 Nebraska winter wheat genotypes and three commercially grown Egyptian spring wheat check cultivars were grown in three replicates in a randomized incomplete block design at two locations for 3 years (2016/2017–2018/2019) in Egypt. Seven traits were measured on all genotypes (the number of days to flowering; total chlorophyll content; plant height; leaf, stem, and stripe rust reaction; and grain yield). The winter wheat genotypes flowered and several of them were not significantly different in grain yield from the spring cultivars. A multi‐trait stability index identified 22 genotypes (20% selection intensity, the local checks, and 19 lines from the United States) for good performance. This study provides novel evidence that winter wheat can flower in northern Egypt, can be used for adult plant resistance screening, and provide a grain yield comparable to the commercially grown spring wheat cultivars. Our findings are expected to facilitate future efforts to hybridize winter‐by‐spring lines to develop new genotypes with unique combinations of genes to improve grain yield production, adaptation, disease resistance, and climate resiliency.

Registration of ‘Pembroke 2021’ soft red winter wheat

AbstractKentucky's wheat (Triticum aestivum L.) crop is planted into a rotation in which corn (Zea mays L.) is followed by wheat, which is followed by double‐crop soybean [Glycine max (L.) Merr.]. The causal agent of Fusarium head blight (FHB), Fusarium graminearum Schwabe, overwinters in corn stubble; thus, the Kentucky wheat crop is always at risk for FHB. The preeminent goal of the University of Kentucky wheat breeding program is releasing FHB‐resistant winter wheat cultivars. ‘Pembroke 2021’ (Reg. no. CV‐1198, PI 701381) is an early‐maturing, semi‐dwarf, soft red winter wheat cultivar developed and released in 2020 by the Kentucky Agricultural Experiment Station for FHB resistance, high yield and test weight potential, and lodging resistance. Pembroke 2021, tested as KY 07C‐1145‐94‐12‐5, was developed from the cross ‘IL99‐15867’/B990081//KY97C‐0554‐04‐05. The pedigree of the KY parent is VA94‐54‐549/Roane//Kristy. Pembroke 2021 was named for the Pembroke silt loam soil series prevalent in the main wheat‐producing area of Kentucky. Pembroke 2021 was selected from F4:5 head rows using a modified bulk breeding method; selected rows carried the resistance allele at the following FHB resistance quantitative trait loci: 1B Jamestown, 1A Neuse, 4A Neuse, and 3B Massey. Pembroke 2021 was tested in Kentucky in multi‐location replicated yield trials, the Uniform Eastern Soft Red Winter Wheat Nursery, the Mason‐Dixon nursery, the Uniform Northern Winter Wheat Scab Nursery, and the Kentucky Wheat Variety Trial.

Genetic Basis of Resistance to Warrior (-) Yellow Rust Race at the Seedling Stage in Current Central and Northern European Winter Wheat Germplasm.

To evaluate genetic variability and seedling plant response to a dominating Warrior (-) race of yellow rust in Northern and Central European germplasm, we used a population of 229 winter wheat cultivars and breeding lines for a genome-wide association study (GWAS). A wide variation in yellow rust disease severity (based on infection types 1-9) was observed in this panel. Four breeding lines, TS049 (from Austria), TS111, TS185, and TS229 (from Germany), and one cultivar, TS158 (KWS Talent), from Germany were found to be resistant to Warrior (-) FS 53/20 and Warrior (-) G 23/19. The GWAS identified five significant SNPs associated with yellow rust on chromosomes 1B, 2A, 5B, and 7A for Warrior (-) FS 53/20, while one SNP on chromosome 5B was associated with disease for Warrior (-) G 23/19. For Warrior (-) FS 53/20, we discovered a new QTL for yellow rust resistance associated with the marker Kukri_c5357_323 on chromosome 1B. The resistant alleles G and T at the marker loci Kukri_c5357_323 on chromosome 1B and Excalibur_c17489_804 on chromosome 5B showed the largest effects (1.21 and 0.81, respectively) on the severity of Warrior (-) FS 53/20 and Warrior (-) G 23/19. Our results provide the basis for knowledge-based resistance breeding in the face of the enormous impact of the Warrior (-) race on wheat production in Europe.

Spore-Trapping Device: An Efficient Tool to Manage Fungal Diseases in Winter Wheat Crops.

Leaf airborne diseases cause major shortfalls in agricultural crops. The introduction of technical means can significantly improve early-warning systems for plant diseases as well as provide timely and accurate forecasts. In this paper, we aim to evaluate the possibilities of detecting a phytopathogenic infection using a spore-catching device developed at the Federal Research Center of Biological Plant Protection (FRCBPP) on winter wheat varieties of different levels of susceptibility to major economically important leaf diseases, taking into account climatic conditions. The device captures spores in the surface layer of air among crop plants. We conducted research in the experimental fields of FRCBPP in 2019-2021. The objects of the study were four cultivars of winter wheat. They were selected according to the degree of resistance to various leaf diseases. We studied the progress of wheat diseases according to generally accepted international scales the onset of the first manifestations to their maximum development. We studied the aerogenic infection in wheat crops using the FRCBPP developed portable device for determining the infestation of plants. Sampling was carried out in the same period as the visual assessment. The samples were taken in the crops of each variety at five points. The sampling time was one minute. As a result of research on experimental crops of four varieties of winter wheat, we observed the development of such diseases as powdery mildew (Erysiphe graminis), yellow spot (Pyrenophora tritici-repentis), septoria leaf spot (Septoria tritici), yellow (Puccinia striiformis) and brown rust (Puccinia triticina). In a laboratory study of samples under a light microscope, all of the listed pathogens were found except for septoria leaf spot. Two-way analysis of variance confirmed the statistically significant separate and cumulative influence of the cultivar and year factor on winter wheat diseases. A generalized correlation analysis for three growing seasons (2019-2021) showed that an average statistically significant correlation coefficient (0.5-0.6) remains for the total groups for powdery mildew, yellow and brown rust. This indicator for the causative agent of yellow spot was equal to 0.4 with a high level of statistical significance. Thus, we conclude that by using a spore-catching device, it is possible to identify spores of economically significant pathogens in winter wheat crops and predict the further development of pathogens, taking into account the cultivar and annual climate factors.

PERFORMANCE OF SOME ROMANIAN WINTER WHEAT CULTIVARS UNDER ORGANIC AGRICULTURE CONDITIONS I. GRAIN YIELD

In order to identify wheat cultivars most suitable for cultivation in organic agriculture, we tested 16 Romanian winter wheat cultivars in yield tests organized in South Romania at Fundulea and Valului Traian during 2019-2022. Most of the yield variation was due to the variation of environmental conditions, but cultivars also had a significant effect. Older cultivars yielded less than modern ones, and highest average grain yields were obtained in the most recently registered cultivars, Ursita and FDL Abund. Genetic progress for yield achieved in breeding programs performed in conventional agriculture conditions was also reflected in the cultivars’ performance under organic agriculture.

PERFORMANCE OF SOME ROMANIAN WINTER WHEAT CULTIVARS UNDER ORGANIC AGRICULTURE CONDITIONS II. BREAD MAKING QUALITY INDICES

In order to explore genetic possibilities for producing wheat suitable to bread-making industry requirements, we tested 16 Romanian winter wheat cultivars in yield tests organized in South Romania at Fundulea, during 2019-2022. Grain protein concentration was generally low, only line FDL Amurg fulfilling on average, but not every year, the requirements for Grade 1 (in Romania, the requirements for organic wheat are the same as for conventional wheat, although the protein content is generally lower under organic conditions). Most tested cultivars belonged to Grade 3, with average grain protein content below 11%. A significant part of grain protein variation was associated with grain yield variation, but two genotypes (FDL Amurg and Voinic) showed positive deviations from the regression yield – grain protein content. Zeleny sedimentation and the alveograph W index were strongly correlated with grain protein percentage, suggesting that, in order to produce high quality wheat under organic agriculture, improving grain protein concentration, genetically or by crop management, is most important.

Wpływ kwasu krotonowego na skuteczność działania i fitotoksyczność diflufenikanu stosowanego w obniżonych dawkach

The subject of the research was the evaluation of the effect that crotonic acid applied at four doses has on diflufenican activity (at the recommended dose and doses reduced by 50 and 75%) against certain weed species. Three series of experiments were performed in glasshouse conditions. Phytotoxicity of the mixtures of both components was also evaluated against two cultivars of winter wheat. The addition of crotonic acid did not markedly affect the activity of diflufenican against Viola arvensis, Descurainia sophia and Thlaspi arvense. Mixtures of crotonic acid in the tested doses and diflufenican at a 50% reduced dose destroyed Apera spica-venti even more effectively than the recommended dose of the herbicide. At the highest rate (5.4 kg/ha) crotonic acid significantly improved the efficacy of diflufenican at the reduced by 50% dose in destroying Papaver rhoeas and Stellaria media. Crotonic acid, diflufenican and the mixtures of these compounds were safe for winter wheat cultivars KWS Dakotana and RGT Bilanz.

Fungicide Efficacy on Fusarium Head Blight of Hard Red Winter Wheat in Parsons, KS

In 2022, a field experiment was conducted to investigate the efficacy of fungicide programs on Fusarium head blight (FHB) and deoxynivalenol (DON) levels. Fungicide treatments were evaluated in plots of the hard red winter wheat cultivar ‘KanMark’ inoculated with Fusarium graminearum in Parsons, KS. Treatments consisted of a single application of Prosaro, Caramba, Miravis Ace, Prosaro Pro, or Sphaerex at early anthesis (Feekes 10.5.1), or dual application of Miravis Ace at early anthesis followed by (fb) Prosaro Pro, Sphaerex, or Folicur at 4 days after early anthesis. All fungicide programs numerically reduced FHB visual symptoms and resulted in increased yield compared to the untreated check, although differences were not statistically significant in all cases. The double application treatments (Miravis Ace fb Prosaro Pro, and Miravis Ace fb Spharex) resulted in the highest increase in test weight. Miravis Ace applied at early anthesis fb Prosaro Pro applied at 4 days after early anthesis resulted in the lowest DON levels (79% less than the untreated check).

Effect of Heavy Metal Stress on Phenolic Compounds Accumulation in Winter Wheat Plants.

Heavy metal stress can lead to many adverse effects that inhibit cellular processes at various levels of metabolism, causing a decrease in plant productivity. In response to environmental stressors, phenolic compounds fulfill significant molecular and biochemical functions in plants. Increasing the biosynthesis of phenolic compounds in plants subjected to heavy metal stress helps protect plants from oxidative stress. A pot experiment was carried out to determine the effect of the accumulation of copper (Cu) and lead (Pb) salts at concentrations of 200, 500, and 1000 ppm on seed germination, the activity of enzymes in the phenylalanine ammonia-lyase pathway (PAL) and tyrosine ammonia-lyase (TAL), along with the total phenol and flavonoid contents in seedlings of hybrid Triticum aestivum L. (winter wheat) cultivars. The accumulation of heavy metals, especially Cu, had a negative impact on the seed germination process. The cultivar "Hyacinth" reacted most strongly to heavy metal stress, which was confirmed by obtaining the lowest values of the germination parameters. Heavy metal stress caused an increase in the activity of PAL and TAL enzymes and an increase in the accumulation of phenolic compounds. Under the influence of Cu, the highest activity was shown in cv. "Hyvento" (especially at 200 ppm) and, due to the accumulation of Pb, in cv. "Hyacinth" (1000 ppm) and cv. "Hyking" (200 ppm). The cultivar "Hyking" had the highest content of phenolic compounds, which did not increase with the application of higher concentrations of metals. In other cultivars, the highest content of total phenols and flavonoids was usually observed at the lowest concentration (200 ppm) of the tested heavy metals, Cu and Pb.

Mapping and validation of all-stage resistance to stem rust in four South African winter wheat cultivars

Mapping and validation of all-stage resistance to stem rust in four South African winter wheat cultivars

Effect of tillage and macronutrients application on the quantitative and qualitative parameters of winter wheat (Triticum aestivum) under semi-arid conditions of Southern Russia

Tillage system and macronutrients are the many emerging farming issues that are addressed in contemporary cropping systems. In this regard, selection of a promising high-yielding variety of winter soft wheat was considered. The effects of basic tillage practices (Moldboard ploughing, Flat cutting ploughing and Disk ploughing) in combination with the different fertilization (Fertilizer-free, N90P50 and N180P100) were investigated on qualitative and quantitative parameters of winter wheat cultivar Grom under semi-arid environmental condition of southern Russia. The experimental design used in this experiment was a randomized complete block design (RCBD) with three replications. The highest winter wheat Grom yield with the value of 5.3 and 5.6 t/ha was attained for Moldboard ploughing and Flat cutting ploughing at a depth of 20-22 cm combined with application of nutrients N180P100, respectively. On the other hand, the lowest grain yield (2.78 t/ha) was achieved in fertilizer-free treatment in combination with Disk ploughing 12-14 cm depth. Moldboard ploughing plus nutrient application N180P100 had the best results on leaf area (48.2 thousand m2/ha), photosynthetic potential (million m2 × day/ha) and dry biomass (10.1 t/ha).

A Haplotype-Based GWAS Identified Trait-Improving QTL Alleles Controlling Agronomic Traits under Contrasting Nitrogen Fertilization Treatments in the MAGIC Wheat Population WM-800.

The multi-parent-advanced-generation-intercross (MAGIC) population WM-800 was developed by intercrossing eight modern winter wheat cultivars to enhance the genetic diversity present in breeding populations. We cultivated WM-800 during two seasons in seven environments under two contrasting nitrogen fertilization treatments. WM-800 lines exhibited highly significant differences between treatments, as well as high heritabilities among the seven agronomic traits studied. The highest-yielding WM-line achieved an average yield increase of 4.40 dt/ha (5.2%) compared to the best founder cultivar Tobak. The subsequent genome-wide-association-study (GWAS), which was based on haplotypes, located QTL for seven agronomic traits including grain yield. In total, 40, 51, and 46 QTL were detected under low, high, and across nitrogen treatments, respectively. For example, the effect of QYLD_3A could be associated with the haplotype allele of cultivar Julius increasing yield by an average of 4.47 dt/ha (5.2%). A novel QTL on chromosome 2B exhibited pleiotropic effects, acting simultaneously on three-grain yield components (ears-per-square-meter, grains-per-ear, and thousand-grain-weight) and plant-height. These effects may be explained by a member of the nitrate-transporter-1 (NRT1)/peptide-family, TaNPF5.34, located 1.05 Mb apart. The WM-800 lines and favorable QTL haplotypes, associated with yield improvements, are currently implemented in wheat breeding programs to develop advanced nitrogen-use efficient wheat cultivars.

Evaluation of two artificial defoliation methods to simulate damage by the cereal leaf beetle (Oulema melanopus) larvae in winter wheat

AbstractDefoliation reduces photosynthetic area, negatively effecting overall plant vitality, which at the end, severely impacts seed quality and production. The economic importance of the loss in winter wheat (Triticum aestivum L.) due to larvae of the cereal leaf beetle (Oulema melanopus, CLB) generated studies investigating the significance of the flag leaf. Simultaneously, the role of other leaves remains rather undiscovered. We simulated herbivory caused by CLB larvae in a two-year study between 2017 and 2018. We removed different amounts of leaf material from two winter wheat cultivars, either from the flag leaves only, or from all leaves. The impact of artificial defoliation was measured in grain production per ear, and related to natural CLB larval herbivory. Removing all leaves simulated CLB larval herbivory more closely than the artificial defoliation of flag leaves only. Our results suggest that the relative importance of flag leaves in seed production may be lower than previously assumed. Further studies involving various cultivars are invited to enhance the knowledge on the significance of the damage done by CLB larvae.

Bread-Making Potential and Yielding of Hybrid Wheat Under Varied Agronomic and Environmental Factors

Abstract The purpose of this study was to determine the effect of simplified tillage systems (RT, NT) in comparison with conventional tillage (CT) on technological grain quality and baking value, as well as of hybrid wheat grown under variable weather conditions. A three-year field trial experiment was conducted using a randomised block design, in triplicate. The factors studied were as follows: I - three tillage systems - no-tillage (NT), reduced (RT), and conventional (CT); II - two winter wheat cultivars ‘Hylux’ (cv. hybrid) and for comparison ‘Bogatka’ (cv. common). The use of CT and RT tillage systems compared to NT significantly increased yield by 8.9 and 7.7%, respectively, and selected grain quality parameters along with a more favorable gluten protein profile. The flour obtained, with water absorption above 58.0%, can be classified as strong flour with good farinographic and alveograph parameters. The genetic characteristics of the wheat cultivars determined the technological quality of the grain and the baking value, as well as the grain yield. For ‘Hylux’ cv. hybrid wheat, there was a higher yield and better grain quality, which contained significantly more gliadins and glutenins, and the flour was characterised by higher parameters that determine the preferred viscoelastic properties of the dough. The common ‘Bogatka’ wheat cv. accumulated higher contents of ω gliadins and LMW and HMW glutenin subunits in the grain, while for α/β and γ gliadin contents the difference was not significant. A fairly dry period (June–July) of wheat ripening reduced the grain yield but was favourable for higher values of quality characteristics, gluten protein fractions, as well as the farinographic (WAF, DDT, DS) and alveograph (W, P, L) parameters.

Allelotoxicity and Water Stability of Sod-Podzolic Soil after Growing Wheat on It.

It is well-known that soil allelotoxicity and the water stability of soil structure are determined by the entry of plant residues into the soil, but the relationship of these soil properties has not been investigated. Soil samples from the fields of the Federal Research Center "Nemchinovka" after growing 25 cultivars of spring and winter wheat on plots with sod-podzolic soil are selected in this study. The effect of the soil's allelotoxicity of plot samples on the germination of spring wheat seed of the Liza cultivar is studied by the biotesting method. The water stability of the soil structure is determined for the same samples. The existence of a sigmoid dependence between the allelotoxicity of soil samples and their water stability with a correlation greater than 95% is established. The presence of this dependence is apparently related to the increase in the soil's water stability with an increase in the amount of fresh plant residues entering them, which is known from the literature, and the transition of the allelotoxins' effects from stimulation to suppression with a growth in their concentration in soils.

Effects of Fhb1, Fhb2 and Fhb5 on Fusarium Head Blight Resistance and the Development of Promising Lines in Winter Wheat.

The development of Fusarium head blight (FHB)-resistant winter wheat cultivars using the gene Fhb1 has been conducted in northern China. Sumai 3, a Chinese FHB-resistant spring wheat cultivar, carries three FHB resistance genes: Fhb1, Fhb2 and Fhb5. To better use these genes for increasing FHB resistance in northern China, it is necessary to elucidate the pyramiding effects of Fhb1, Fhb2 and Fhb5 in winter wheat backgrounds. Eight gene combinations involving Fhb1, Fhb2 and Fhb5 were identified in a double haploid (DH) population, and the effects on FHB resistance were evaluated in six tests. At the single gene level, Fhb1 was more efficient than the other two genes in single-floret inoculation tests, whereas Fhb5 showed better resistance than Fhb1 and Fhb2 under a natural infection test. Pyramiding Fhb1, Fhb2 and Fhb5 showed better FHB resistance than the other gene combinations. Forty-nine DH lines showing consistently better resistance than the moderately susceptible control Huaimai 20 in multiple tests were evaluated for main agronomic traits, and no difference in grain yield was found between the mean values of DH lines and the recipient parents Lunxuan 136 and Lunxuan 6, which are higher than those of recipient parent Zhoumai 16 and the donor parent Sumai 3 (p < 0.05). Based on the phenotypic and genomic composition analyses, five promising DH lines fully combined the FHB resistance of donor Sumai 3 and the elite agronomic traits from the recipient parents. This study elucidates the pyramiding effects of three FHB resistance genes and that the promising DH lines with resistance to FHB can be directly applied in wheat production or as parents in winter wheat breeding programs.

Does Belt Uniform Sowing Improve Winter Wheat Yield under High Sowing Density?

The belt uniform (BU) sowing pattern can improve the yield of winter wheat, but whether and how the BU sowing pattern can increase yield under different sowing densities is unknown. The field study was conducted in Guiyang (Guizhou province) during the growing season in 2017–2018, 2018–2019, and 2019–2020. Four winter wheat cultivars were used in field experiments to investigate the changes of the dry matter accumulation and partition, yield and yield components at maturity under five sowing densities (75, 150, 225, 300, and 375 plants per m2), and three sowing patterns: line and dense (LD) sowing with 33.3 cm row spacing (LDS); the belt uniform (BU) sowing with 15 cm (BUN), and 20 cm (BUW) row spacing. The BU sowing pattern significantly increases shoot dry matter and grain yield in all four winter wheat cultivars under all five sowing densities and in each growing season, particularly under the high sowing density of 300 and 375 plants m−2. Harvest index was unaffected by the different sowing densities and sowing patterns. While spike number increased, grain weight per spike decreased with the increase in sowing density. The 1000-grain weight and grain number per spike were unaffected by the sowing patterns. The variation in the shoot dry weight can explain 94% variation in grain yield and 66% variation in spike number. Allometric analysis showed that more dry matter was partitioned to the spike than to the stem and leaf. We conclude that the BU sowing pattern can increase grain yield under high sowing densities associated with a high shoot dry matter accumulation and its partition to the spike.