An inland river grassland basin is a fragile ecosystem, and precipitation is of great significance to its resilience. However, the knowledge of where precipitation-forming water vapor originated is not well understood, limiting the predictability of future precipitation provided climate change. The objective of this study was to characterize the hydrogen (δD) and oxygen (δ18O) isotopes and hydro-chemical compositions of precipitation as well as decipher atmospheric water vapor sources in a typical Eurasian steppe river basin. In this regard, this study collected 59 water samples from 44 storm events in 2019 and 2020. The samples were analyzed in our laboratory for ions, δD, and δ18O, which in turn were used to examine the stable isotopic features, hydro-chemical characteristics, and water vapor transport at different temporal scales by a backward trajectory model and correlation and redundancy analyses. The results showed negative δD and δ18O during the wet season with a trend of positive deuterium excess, indicating that precipitation effect was significant. In comparison with a linear model of global meteoric water levels, the local meteoric water lines had smaller slopes and intercepts, with a maximum slope in August and a minimum slope in July. In addition, HCO3− and Ca2+ were the primary hydro-chemical ions in precipitation. Ca2+ and Mg2+ were significantly correlated with HCO3− and Na+, indicating that they shared terrestrial and marine sources. NH4+ originated mainly from human activities and chemical reactions, whereas K+ originated primarily from continental and marine processes. Further, the atmospheric water vapor was mainly transported from the northeast, northwest, and southeast directions into the study basin, as affected by the westerly wind circulation, the East Asian summer monsoon, northeast anticyclone circulation, and local evapotranspiration. Moreover, the water vapor was from both onsite and offsite sources: over 40% from Eurasia, 10 to 25% from the Arctic Ocean, and 35 to 45% from the Pacific Ocean.
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