Wild-living Animals Research Articles

Monitoring of Group A Rotaviruses in Wild-Living Birds in Hungary

Rotavirus is a common pathogen causing gastroenteritis in humans and domesticated animals. The incidence of rotavirus in wild-living animals, particularly in avian species, has not been systematically investigated. In this study 1220 fecal samples and cloacal swabs collected from wild-living birds during 2008 in Hungary were tested for the presence of group A rotaviruses by a VP6 gene-specific reverse-transcription-polymerase-chain-reaction assay. Of the 1220 samples, 276 and 944 were processed as individual and pooled specimens, respectively. Rotavirus was identified in two pooled pheasant (Phasianus colchicus) samples and two individual reed bunting samples (Emberiza schoeniclus). These data indicated a very low prevalence of group A rotaviruses (0.3%) in our sample set. Nonetheless, the present study, together with existing literature data, implies that rotavirus infections occur in a wide spectrum of feral bird species. These findings are exciting and suggest that pursuing rotavirus monitoring is needed to uncover avian rotavirus strain diversity and understand rotavirus ecology in nature.

LC–MS analysis of androgen metabolites in serum and urine from east African chimpanzees ( Pan troglodytes schweinfurthii)

Testosterone regulates a wide variety of behavioral and physiological traits in male vertebrates. It influences reproductive and aggressive behaviors and is used as a marker of gonadal activity. While testosterone is the primary biologically active male gonadal steroid in the blood, it is metabolized into a variety of related steroids when excreted via urine and feces. To monitor endocrinological profiles studies on wild-living animals primarily rely on non-invasively collected samples such as urine or feces. Since a number of androgen metabolites that are found in high concentrations in these matrices do not stem exclusively from gonadal production, but are also produced by the adrenal cortex, the metabolism and excretion pattern of testosterone and its characteristic metabolites have to be investigated. Here, we compare the levels of 11 androgens and their metabolites in serum and urine (after hydrolytic/solvolytic cleavage of conjugates) from female, and intact and castrated male chimpanzees to investigate whether they were of testicular or adrenal origin. For serum, significant differences in concentrations were found only for native testosterone. For urine, testosterone concentrations showed the largest differences between intact and castrated males, and intact males and females, while no differences were seen between females and castrated males. Epitestosterone levels revealed the same pattern. These differences in urinary concentrations could also be seen for 5α-androstane-3α,17β-diol (androstanediol), and less clearly for 5α-dihydrotestosterone (5α-DHT), etiocholanolone, and androsterone. In urine of males, significant correlations were found between the levels of testosterone and 5α-androstane-3α,17β-diol, as well as between testosterone and epitestosterone. Therefore, the clearest urinary markers of gonadal activity in male chimpanzees seems to be testosterone itself.

Natural History of Innate Host Defense Peptides.

Host defense peptides act on the forefront of innate immunity, thus playing a central role in the survival of animals and plants. Despite vast morphological changes in species through evolutionary history, all animals examined to date share common features in their innate immune defense strategies, hereunder expression of host defense peptides (HDPs). Most studies on HDPs have focused on humans, domestic and laboratory animals. More than a thousand different sequences have been identified, yet data on HDPs in wild-living animals are sparse. The biological functions of HDPs include broad-spectrum antimicrobial activity and immunomodulation. Natural selection and coevolutionary host-pathogen arms race theory suggest that the extent and specificity of the microbial load influences the spectrum and potency of HDPs in different species. Individuals of extant species-that have lived for an extended period in evolutionary history amid populations with intact processes of natural selection-likely possess the most powerful and well-adapted "natural antibiotics". Research on the evolutionary history of the innate defense system and the host in context of the consequences of challenges as well as the efficacy of the innate immune system under natural conditions is therefore of immediate interest. This review focuses on evolutionary aspects of immunophysiology, with emphasis on innate effector molecules. Studies on host defense in wild-living animals may significantly enhance our understanding of inborn immune mechanisms, and help identify molecules that may assist us to cope better with the increasing microbial challenges that likely follow from the continuous amplification of biodiversity levels on Earth.

Entry of Heavy Metals into Food Chains: a 20-year Comparison Study in Northern Moravia (Czech Republic)

The aim of the study presented here was to assess cadmium, lead and mercury contamination of wild-living animals and cattle in Northern Moravia (Czech Republic). Samples were collected in 2005–2007 at the same locations as in 1986–1989, in the same season of the year, and they were analyzed using the same methods (AAS ). In 2005–2007, a significant decrease (P < 0.01) in cadmium concentrations in plant fodders was found, while changes in lead and mercury concentrations were less marked, and they depended on the type of commodity. In the 2005–2007 period, a marked decrease (P < 0.01) in cadmium concentrations in the muscle and kidneys of the cattle, fallow deer, roe deer and pheasants (muscle 0.002–0.009 mg kg-1, kidneys 0.11–0.69 mg kg-1) was found. In the same period, mercury concentrations in the muscle, liver and kidneys were significantly lower (P < 0.01) in cattle, roe deer and hare tissues (muscle: 0.6–3.5 μg kg-1, liver 3.3–41.0 μg kg-1, kidney 16.3–43.2 μg kg-1). Lower lead concentrations in the 2005–2007 period were only found in cows (0.04 mg kg-1, 0.1 mg kg-1 and 0.6 mg kg-1 in muscle, liver and kidney samples, respectively). No significant differences in lead concentrations were found in the tissues of other animals. The study demonstrated a reduction in the amounts of heavy metals entering food chains in the study area in recent years.

Compatibility counts: MHC-associated mate choice in a wild promiscuous primate

The mechanisms and temporal aspects of mate choice according to genetic constitution are still puzzling. Recent studies indicate that fitness is positively related to diversity in immune genes (MHC). Both sexes should therefore choose mates of high genetic quality and/or compatibility. However, studies addressing the role of MHC diversity in pre- and post-copulatory mate choice decisions in wild-living animals are few. We investigated the impact of MHC constitution and of neutral microsatellite variability on pre- and post-copulatory mate choice in both sexes in a wild population of a promiscuous primate, the grey mouse lemur (Microcebus murinus). There was no support for pre-copulatory male or female mate choice, but our data indicate post-copulatory mate choice that is associated with genetic constitution. Fathers had a higher number of MHC supertypes different from those of the mother than randomly assigned males. Fathers also had a higher amino acid distance to the females' MHC as well as a higher total number of MHC supertypes and a higher degree of microsatellite heterozygosity than randomly assigned males. Female cryptic choice may be the underlying mechanism that operates towards an optimization of the genetic constitution of offspring. This is the first study that provides support for the importance of the MHC constitution in post-copulatory mate choice in non-human primates.

An ethological approach to housing requirements of golden hamsters, Mongolian gerbils and fat sand rats in the laboratory—A review

When housing hamsters and gerbils little has been done to identify the preferences of these species. The solitary nature of fat sand rats and hamsters is often discordant with housing practices in animal laboratories. This article provides a comparison of the behaviour of wild-living animals with that of laboratory housed conspecifics, focusing on possible problems related to social structure and enrichment. More studies are needed to reveal the impact of group-housing on hamsters and fat sand rats, just as preference studies on possible enriching resources needs to be undertaken in order to optimise the housing conditions of laboratory hamsters, gerbils and fat sand rats.

Grünbrücke Loterbuck A 4.2.9: Eine Erfolgskontrolle nach drei Jahren | Fauna Overbridge Loterbuck A 4.2.9: Success Control after a Period of Three Years

The expansion of the traffic network, in particular the construction of highways, has continuously diminished and divided into small sections the habitat of wild-living animals during the last decades. However, these negative effects can be minimised if suitable measures with regard to line-conduction and construction are taken against. One of these possibilities are the sown-down overbridges. It is essential that these constructions are planned and built in order to meet the requirements of their future users – the various wild-living animals. This study investigates the success of one of these sown-down overbridges. It is this the Loterbuck-overbridge on the A 4.2.9 near Henggart in the canton of Zurich, Switzerland. The focus of this investigation was to find out which species of wild-living animals use the bridge and how much it is frequented. Local people and specialists of the region were interviewed and tracks were picked up on site. Taking into consideration five criteria (species of wild-living animals, positioning and number of overbridges nearby, dimensioning and design of the individual overbridges), the interviews and tracks were assessed. The Loterbuck-overbridge is used by all larger wild-living animals of the region. Especially the browsing and rubbing tracks of deer show that the overbridge has been accepted not only as sown-down overbridge but also as habitat.

Conditioned taste aversion to saccharin induced by 2, 4, 5-trichlorophenoxyacetic acid in albino rats

Four groups of Wistar rats were exposed either to saccharin + 2,4,5-T (taste-aversion group, T-A), saccharin + oil vehicle (taste-aversion control, T-A C), water + 2,4,5-T (enhanced neophobia, E-N), or water + oil (neophobia, N). Rats in the T-A group evidenced a marked aversion to saccharin in 3 consecutive preference tests, performed every third day, starting 3 days after exposure. The aversion was less pronounced, although statistically significant in 2 additional preference tests, performed respectively after a 9-day rest period of ad lib water drinking, and a 24 hr period of forced exposure to saccharin. An enhanced neophobia effect was found in the E-N group in the first preference test. Suggestions are made concerning the possible long-term effects on food preferences among wild-living animals as a result of large-scale application of 2,4,5-T-containing herbicides.