Daucus Carota Research Articles

Nitrogen availability modulates carotene biosynthesis, chromoplast biogenesis, and cell wall composition in carrot callus.

Carrot callus grown on a medium with increased nitrogen have reduced carotenoid accumulation, changed gene expression, high amount of vesicular plastids and altered cell wall composition. Carotenoid biosynthesis is vital for plant development and quality, yet its regulation under varying nutrient conditions remains unclear. To explore the effects of nitrogen (N) availability, we used carrot (Daucus carota L.) model callus cultures in vitro as a controlled system for studying nutrient-regulated metabolic processes. Two mineral media differing in N content and NO₃⁻/NH₄⁺ ratios were used. Comprehensive analyses, HPLC, transmission electron microscopy, immunochemistry, and RNA sequencing, revealed notable cellular and molecular responses to N treatments. The results demonstrated that N supplementation reduced carotenoid content by 50%, particularly β-carotene and α-carotene. The composition of chromoplast types shifted, with vesicular chromoplasts dominating (55%), followed by a globular type (23%), while in the control callus, globular and crystalline types predominated (57% and 33%, respectively). Immunohistochemistry showed increased presence of high-esterified pectins and arabinogalactan proteins in N-treated cells. Transcriptomic analysis identified 1704 differentially expressed genes (DEGs), including only two in the carotenoid biosynthesis pathway: phytoene synthase 2 (PSY2) and zeaxanthin epoxidase (ZEP). PSY2, which encodes the carotenoid rate-limiting enzyme, showed expression levels that corresponded with reduced carotene content. Other DEGs included 15 involved in nitrogen transport, 1 in nitrogen assimilation, 40 in cell wall biosynthesis and modification, and 9 in phenylpropanoid/flavonoid pathways. N-treated callus exhibited altered expression of MADS-box, NLP, bZIP, and ethylene-responsive transcription factors. These findings reveal how nitrogen availability disrupts carotenoid biosynthesis and triggers extensive chromoplast and cell wall remodeling, providing a cellular framework for understanding nutrient-regulated metabolic shifts.

Role of Carrot (Daucus carota L.) Storage Roots in Drought Stress Adaptation: Hormonal Regulation and Metabolite Accumulation

Role of Carrot (Daucus carota L.) Storage Roots in Drought Stress Adaptation: Hormonal Regulation and Metabolite Accumulation

Plasma Lipid Lowering Potential of Carrot (Daucus carota) Extract in Male Wistar Rats

Carrots (Daucus carota) are widely consumed for their nutritional and medicinal benefits. The aim of the present study was to investigate the effects of Daucus carota on lipid profile. The study involved 28 male wistar rats separated into 4 groups of 7 rats each. Group 1 served as control and was given distilled water, whilst groups 2, 3 and 4 served as test groups and were given aqueous extract of Daucus carota at daily doses of 200mg/kg, 400mg/kg and 600mg/kg respectively. The experiment lasted for 28 days and thereafter, blood samples collected for determination of lipid profile [total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL)] using standard laboratory techniques. Results showed that oral administration of all three doses of carrot extracts significantly reduced the plasma concentrations of both TC and TG but caused no significant changes in the LDL levels. However, the higher doses; 400mg/kg and 600mg/kg significantly increased the HDL levels in wistar rats. The lipid lowering potential of Daucus carota could be based on its fibre content as well as its antioxidant content which possibly enhanced fecal excretion of lipids. Conclusively, moderate consumption of Daucus carota decreased the plasma levels of TC and TG, increased HDL but had no significant effect on the LDL.

Green synthesis of iron oxide nanoparticles from extracts of Daucus carota subsp. Sativus whole vegetable, peel, pomace, and juice and their application as an antibacterial agent and Fenton-like catalyst

Green synthesis of iron oxide nanoparticles from extracts of <i>Daucus carota subsp. Sativus</i> whole vegetable, peel, pomace, and juice and their application as an antibacterial agent and Fenton-like catalyst

Silver Nanoparticles for Anticancer and Antibacterial Therapy: A Biogenic and Easy Production Strategy

AbstractMetal nanoparticles are very valuable products due to their wide range of uses. Among these silver nanoparticles are beneficial products used in many fields, especially in medicine, due to their antibacterial properties. This research aimed to produce silver nanoparticles (Ag NPs) that are both affordable and environmentally friendly. For this purpose, Ag NPs were quickly obtained from domestic waste components of the carrot plant (Daucus carota L.). The UV–vis spectrophotometric, TEM, AFM, FE‐SEM, STEM, EDX, XRD, and DLS analyses were performed to determine the properties of the obtained Ag NPs. It has been found that their surface charge is −21.8 mV, with a maximum absorbance at a wavelength of 421.37 nm, spherical appearance, and an average size distribution of 85.41 nm. The anticancer and antibacterial activities of the produced Ag NPs were investigated by MTT and microdilution. The synthesized Ag NPs showed the most significant antimicrobial effect on Pseudomonas aeruginosa ATCC 27833 with microdilution and low concentration. However, they were also determined to be effective on Bacillus subtilis ATCC 11774 and on Candida albicans ATCC 10231 pathogenic strains. In fact, the effective concentrations of Ag NPs on these strains were significantly lower than the antibiotics used. Furthermore, aside from exhibiting a superior anticancer impact on CaCO‐2 cancer cells, it was established that Ag NPs also had remarkable efficacy in inhibiting U118 and Skov‐3 cancer cells.

Effect of Daucus carota subsp. sativus on pigmentation and profitability of Cobb 500 broiler chicken

Background: The quality of chicken meat, essential for consumer satisfaction, is influenced by skin pigmentation. The lack of carotenoids in conventional diets motivates the search for cost-effective alternatives to enhance these aspects in poultry production. Objective: This study assessed the impact of partially replacing commercial balanced feed with different levels of carrot flour (Daucus carota subsp. sativus) on productive parameters and the pigmentation of broiler chickens. Methods: 64 Cobb 500 birds were randomly assigned to four experimental diets, each with eight replicates of two birds. The control group (T1) received a standard diet, while experimental groups received a diet with 10% (T2), 15% (T3), and 20% (T4) replacement with carrot flour. Variables such as feed consumption, weight gain, feed conversion, mortality, skin pigmentation, and profitability were evaluated. Results: Treatments with 15% and 20% carrot flour showed higher feed consumption. Birds in experimental groups exhibited higher weight gain and improved feed conversion, with no reported mortality. Regarding pigmentation, carrot flour influenced skin color as its concentration in the diet increased. In terms of profitability, the 15% replacement treatment stood out by generating higher income and a superior cost-benefit ratio. Conclusion: Orange cultivar carrot flour can be a viable dietary source of natural pigment for broiler chickens. It may also be beneficial in promoting weight gain and reducing mortality, translating into economic advantages.

Bio-Preparation of Iron Nano Fertilizers from Lemon Fruit Affected some of the Characters of Carrot plants (Daucus carota L.)

To study the biological preparation of iron nano fertilizers from lemon fruit extract and their effect on some quantitative traits of carrot (Daucus carota L.) plant. Experiments were conducted, a laboratory part and a field part, with two addition factors, foliar fertilization and soil fertilization, using two sources of iron. The first source was nano-iron at a concentration of 0.5, 1, 1.5, and 2 mg L-1, and the second source was chelated iron at a concentration of 1.5 g L-1 and control treatment. The results showed the superiority of foliar fertilization in most of the studied traits such as iron concentration, root length, root diameter, fresh weight, dry weight, carotene and total dissolved solids (144.30 ppm, 17.10 cm, 2.1 cm, 137.98 g, 16.03 g, 1.62 mg g fresh weight-1 and 8.79 %, respectively). Nano-iron spraying was superior than to chelated iron in the concentration of iron (Fe), root length, root diameter, fresh weight, dry weight, and total dissolved solids (1059.22 ppm, 20.82 cm, 2.29 cm, 168.97 g, 18.37 g, and 9.91 %, respectively). X-ray diffraction (XRD) was used to determine the size and purity of the nanoparticles, and scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were used to verify the composition. Iron oxide (Fe2O3) was in the form of α-Fe2O3 hematite with a size of 32.8 nm and Ỵ-Fe2O3 maghematite with a size of 44.6 nm. We conclude that the use of green synthesis method for nano-fertilizers has proven effective and efficient from an economic and environmental point of view.

Developing High-Coloring Natural Systems Using Double Emulsions with Daucus carota L. Extract to Meet High-Performance Requirements.

Daucus carota L. extract is attracting interest as a natural colorant alternative. However, the presence of anthocyanins (ACNs), which are sensitive to pH changes, limits its application. To tackle this issue, water-in-oil-in-water (W1/O/W2) double emulsions are emerging as innovative solutions. Nevertheless, the problem of reaching robust colorant systems for industrial use still needs to be overcome. One important target is to reach a high coloring power, minimizing its impact on the final product. In this context, the effect of colorant concentration and the volume of the primary emulsion, two routes to increase the colorant power, on color attributes and stability, an important feature to reach a marketable product, was studied. The optimal experimental design was conducted to two optimal solutions, whether through heightened colorant concentration or primary emulsion volume: a 41/59 (W1/O)/W2 ratio with 11 wt.% colorant, and a 48/52 (W1/O)/W2 ratio with 6 wt.% colorant, respectively. A subsequent assessment of color and physical emulsion stability over 30 days pointed out the solution with the lower colorant concentration (6 wt.%) as the one with better performance (L*: 44.11 ± 0.03, a*: 25.79 ± 0.01, D4;3: 9.62 ± 0.1 µm, and CI: 14.55 ± 0.99%), also minimizing the permeability of the colorant to the outer aqueous phase. Overall, these optimized emulsions offer versatile coloring solutions suitable for various industrial applications, such as food matrices and functional cosmetics.

Starch-based composite formulation of chickpea flour and black carrot (Daucus carota l.) pomace in extruded snacks: In vitro gastrointestinal behavior and stability of bioactive compounds.

Black carrot pomace (BCP) is a by-product of colorant production, containing various valuable components with the health promoting effects. In this study, for the first time, BCP was incorporated into a starch-based extruded snack formulation using wheat semolina and corn starch. Total phenolic content, total antioxidant capacity, phenolics, and anthocyanins after processing and in vitro gastrointestinal digestion were investigated, and physical and textural properties of the snack products were determined. Incorporating BCP significantly raised the TPC and antioxidant levels, notably achieving peak levels in snacks enriched with 20 % BCP. The phenolic acids and anthocyanins were increased significantly (48-382 %) (p < 0.05) with simulated gastric digestion whereas no anthocyanins were detected after simulated intestinal digestion. On the other hand, the extrusion process resulted in a negative impact on anthocyanin concentrations, particularly marked by a significant reduction in some cyanidin derivatives indicating the detrimental effect of extrusion on the molecular integrity of anthocyanins under high temperature and pressure, leading to their degradation. In the final product, the snacks displayed lower expansion indices, water absorption index, water solubility index, and lightness; but had higher hardness and redness values compared to the control and turned slightly darker. The study proposes to evaluate BCP as a value-added ingredient that imparts functional properties to foods along with the utilization of starch in the extrusion process. Additionally, the integration of wheat semolina and corn starch contributed to the structural integrity and texture of the extruded snacks, highlighting the importance of polysaccharides in the formulation.

Biosynthesis of elemicin and isoelemicin in Daucus carota leaves.

Volatile phenylpropenes comprise one of the largest groups of plant phenylalanine-derived volatiles that not only possess ecological roles but also exhibit numerous pharmacological activities. Despite their wide distribution in the plant kingdom, biosynthesis of only a small subset of these compounds has been discovered. Here, we elucidated yet unknown steps in the biosynthesis of isoelemicin and elemicin using carrot (Daucus carota subsp. sativus), which produces a wide spectrum of volatile phenylpropenes, as a model system. Comparative transcriptomic analysis combined with metabolic profiling of two carrot cultivars producing different spectrums and levels of phenylpropene compounds revealed that biosynthesis of isoelemicin and elemicin could proceed via the (iso)eugenol-independent pathway, which diverges from the lignin biosynthetic pathway after sinapyl alcohol. Moreover, in planta results showed that two different NADPH-dependent reductases, a newly identified 5-methoxy isoeugenol synthase (DcMIS) and previously characterized (iso)eugenol synthase (DcE(I)GS1), both of which use sinapyl acetate as a substrate, are responsible for the biosynthesis of immediate precursors of isoelemicin and elemicin, respectively. In contrast to penultimate reactions, the final steps in the formation of these phenylpropenes are catalyzed by the same newly characterized methyltransferase, S-adenosyl-l-methionine:5-methoxy(iso)eugenol O-methyltransferase, that methylates the para-hydroxyl group of their respective precursors, thus completing the (iso)eugenol-independent route for the biosynthesis of isoelemicin and elemicin.

Iodine Accumulation and Distribution in Carrots (Daucus carota L.)

Iodine is a critical trace element necessary for human and animal health owing to its role in thyroid hormone synthesis. Despite its importance, iodine deficiency remains a global health concern. Traditional methods to address this issue, such as salt iodization, face challenges like iodine loss during storage and cooking. The biofortification of plants, particularly carrots, offers a promising alternative. This study investigates iodine accumulation and distribution in carrots biofortified with potassium iodide (KI) and potassium iodate (KIO3) using single extraction at elevated temperatures with tetramethylammonium hydroxide followed by iodine determination by ICP-MS. Results show that iodine biofortification significantly increases the iodine content of various parts of the carrot, especially the leaves and the root peels. Carrots treated with iodate accumulate 2.7 times more iodine than those treated with iodide. The leaves exhibit the highest iodine content, particularly in iodate-treated plants, where levels can be over 24 times higher than those of control carrots. Root peel and roots also accumulate substantial amounts of iodine, with the iodate treatment resulting in 5.42 mg·kg−1 and 3.75 mg·kg−1 dry weight, respectively. The potential application of biofortified carrots can help meet human dietary iodine requirements; additionally, iodine-rich carrot leaves as livestock feed offer a sustainable approach to increasing the iodine intake in animals.

Biosynthesis of Daucus carota subsp. Sativus-based metal-doped ferric–biochar–bimetallic nanocomposites for dye extraction

Biosynthesis of Daucus carota subsp. Sativus-based metal-doped ferric–biochar–bimetallic nanocomposites for dye extraction

Addition of Carrot Juice (Daucus carota) to the Shelf Life and Organoleptic of Turkey Meatballs

Turkey is a type of poultry with characteristics and tastes similar to chicken meat, so turkey has the potential to be a raw material for meatballs. Carrots are also a source of natural antioxidants, the use of carrots so far has only been for vegetables and juice. Utilization in a fresh state has limitations because fresh carrots are prone to deterioration in quality. This study aims to determine the effect of adding carrot juice on the shelf life and organoleptic of turkey meatballs. This research was conducted in April 2024 at the Production Laboratory, Animal Husbandry Study Program, Faculty of Agriculture, Universitas Sumatera Utara. The design used in this study was RAL (Completely Randomized Design) with 4 treatments, namely P0 = 0 ml, P1 = 50 ml, P2 = 100 ml, P3 = 150 ml and 5 replications. The parameters used were shelf life and organoleptic color, taste, texture and aroma. The results of this study indicate that the addition of carrot juice can extend the shelf life of turkey meatballs. The best shelf life is in P3 with the addition of 150 ml of carrot juice with a shelf life of 5 days and the lowest shelf life is 1 day

Transcriptome Analysis Reveals the Molecular Mechanisms of Carrot Adaptation to Alternaria Leaf Blight.

Carrot (Daucus carota L.) is an important vegetable crop that is rich in carotenoids and is widely cultivated throughout the world. Alternaria leaf blight (ALB), caused by infection with Alternaria dauci (A. dauci), is the most serious fungal disease in carrot production. Although several quantitative trait loci associated with ALB resistance have been identified, the genetic mechanisms underlying this resistance remain largely unelucidated. The aim of the present study was to clarify the infection mode of A. dauci and examine the molecular mechanisms underlying carrot cultivar adaptation to ALB by RNA sequencing. Microscopic observation revealed that A. dauci invades leaf tissues by entering through stomata, and resistant germplasms may significantly inhibit the infection and colonization of A. dauci. In addition, transcriptomic analyses were performed to detect the key pathways and genes associated with the differential responses between ALB-resistant (HB55) and ALB-susceptible (14088) carrot cultivars. These results suggest that the secondary metabolic process, phenylpropanoid biosynthesis, and tyrosine metabolism might play important roles in the resistance of carrots to A. dauci. Three candidate genes (LOC108208301, LOC108215577, and LOC108224339) that were specifically upregulated in the resistant carrot cultivar 'HB55' after A. dauci infection were identified as the key resistance response genes. These findings provide insights into the resistance mechanism of carrots to ALB, as well as key candidate genes and information on expression regulation for the molecular breeding of carrot disease resistance.

Growth and Yield Performance of Carrot (Daucus carota L.) as Influenced by Organic Manure under Open Field Conditions

Growth and Yield Performance of Carrot (Daucus carota L.) as Influenced by Organic Manure under Open Field Conditions

Disentangling mechanisms by which microplastic films affect plant-soil systems: physical effects of particles can override toxic effects of additives

BackgroundMicroplastics, polymer-based particles < 5 mm, affect plant–soil systems positively or negatively, suggesting there are different modes of action. Microplastics, as particles, have physical effects but the leaching of additives likely contributes chemical mechanisms, both of which may be dependent on microplastic size. To disentangle such mechanisms, we established a controlled experiment involving polypropylene and polyethylene films of small, medium and large size, and we evaluated the individual and combined effect of plastic particles and additives (leachates from plastic particles) on soil properties and plant performance of the phytometer Daucus carota and on bare soils.ResultsWe find that additives better explained variation in soil properties (e.g., 44.6% vs 1.3%). Soil respiration and aggregation were negatively affected for additives, likely due to the presence of toxic substances. Overall, such effects increased as plastic size decreased. By contrast, plastic particles better explained plant biomass responses. The positive effect of particles on aeration which may promote root penetration and nutrient uptake, and microplastics itself as a source of carbon potentially promoting soil microbial activity, help explain the positive effect of particles on plant biomass. Plants mitigated the negative effects of additives on bare soils while enhancing the positive effects of particles. This improvement was likely linked to an increase in root activity and rhizodeposition, as plastic particles improved soil aeration. The combined effect of additives and particles, which mimics the microplastic found in the soil, mitigated their individual negative effects on plant–soil systems. As the negative effect of additives could have been masked by the positive effects of particles, simply reporting net positive effects would capture only part of the response.ConclusionsAdditives and plastic particles differently affect soil properties and plant biomass. Additives primarily negatively affect soil properties due to toxic substances, while plastic particles enhance plant biomass likely by improving soil aeration. When examining microplastics effects on terrestrial systems (i.e., the combined effect of additives and particles), the negative effect of additives may be masked by the positive effects of plastic particles. Reporting only net positive effects risks overlooking these underlying negative effects. Plants can mitigate the negative impacts of additives and amplify the positive effects of plastic particles. Our study emphasizes the importance of investigating both the individual and combined effects of additives and particles to fully understand and address the impacts of microplastics on terrestrial ecosystems.

Deciphering Carotenoid and Flowering Pathway Gene Variations in Eastern and Western Carrots (Daucus carota L.).

Carrot is a major root vegetable in the Apiaceae owing to its abundant carotenoids, antioxidants, vitamins, and minerals. The modern dark orange western carrot was derived from sequential domestication events from the white-rooted wild form to the pale orange-, purple-, or yellow-rooted eastern carrot. Genetic and molecular studies between eastern and western carrots are meager despite their evolutionary relatedness. Twelve RNA seq libraries obtained from distinct eastern and western cultivars at vegetative and reproductive developmental stages were utilized to identify differentially expressed genes (DEGs) to decode the key molecular genetic changes in carotenoid and flowering pathways. In the carotenoid pathway, an upregulation of the PSY, CRTISO, and LCYE genes was observed in the western cultivar, while the eastern cultivar exhibited a higher abundance of downstream enzymes, particularly CCD and NCED1. These later enzymes are crucial in linking apocarotenoids and xanthin-mediated ABA signaling. In the flowering pathway, we noted a greater expression of DEGs associated with the photoperiod and vernalization pathways in the western cultivar. In contrast, the eastern cultivar displayed a dominance of genes from the autonomous pathway (FLD, LD, FLK, and PEBP) that function to repress FLC. The experimental validation of 12 key genes through quantitative real-time PCR further confirms their functional role in carrots. The identified key regulatory genes in these major pathways are valuable for designing breeding strategies for manipulating carotenoid content and flowering time while developing climate-specific carrots. The knowledge of carotenoid and flowering pathways is advantageous in producing nutritionally improved roots and seeds in carrots across diverse climates.

Effect of Using Fermented Carrot Leaves in Rations on Organic Matter Consumption, Crude Protein, and Crude Fiber in Thin-Tailed Sheep

Agricultural waste in the form of carrot leaves (Daucus carota L) has potential as a source of feed for thin-tailed sheep. Fermented processing of carrot leaves aims to increase feed efficiency, make it last longer and can be used within a certain time. This research aims to add information and references for readers regarding the effect of using fermented carrot leaves (Daucus carota L) in rations on the consumption of organic matter (BO), crude protein (PK), and crude fiber (SK) in thin-tailed sheep. This research was carried out for fifty-six days from December 2022 to January 2023 at Kandang Waris Mendho Farm and at the Integrated Laboratory of Tidar University. The design used was a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications, P1: 60% concentrate + 40% pakchong forage, P2: 60% concentrate + 10% fermented carrot leaves + 30% pakchong forage, P3: 60% concentrate % + fermented carrot leaves 20% + pakchong forage 20%, P4: concentrate 60% + fermented carrot leaves 30% + pakchong forage 10%, P5: concentrate 60% + fermented carrot leaves 40%. The research results showed that fermented carrot leaves had no effect on (P&gt;0.05) BO consumption and PK consumption, but had a significant effect on (P&lt;0.05) SK consumption. The highest SK consumption was in P1 with a consumption value of 102.44 ± 7.74 g. It can be concluded that fermented carrot leaves do not interfere with PK and SK consumption levels.

Rescue and Translocation of Hispaniola Hutia (Plagiodontia aedium) in Pueblo Viejo, Cotuí Mining Concession Area.

Translocation of species is a common practice used in endangered species management and conservation plans, which can have the purpose of establishing new populations which are at risk of being wiped out, increase a species chance of survival or recovery, and to preserve genetic diversity. The present study details the rescue and relocation process of Hispaniola Hutia (Plagiodontia aedium) in the municipality of Cotuí, Dominican Republic. Data are presented for eight individuals, belonging to two different families, detailing capture methods, handling, reintroduction method, and behavior, as well as data on post-release monitoring for both families. It was found that the most efficient capture method was active capture by hand (6/8). The family groups were heterogeneous, although both families had in common the presence of a single adult male. The average weight of the individuals was 1.19 kg. Blood tests were carried out on the animals before being taken to the holding pens and compared with the values of other rodents, showing that hematic values for P. aedium were like those of another hutia species, Cynomys ludovicianus. During the period animals were held in pens before reintroduction, hutias showed trophic preferences for Clusia rosea and Guarea guidonea. The only supplementary food they consumed was Daucus carota. A fight was observed between members of different families during the acclimation period in the holding pen, between a subadult male and another dominant one, evidencing territorial behavior. Post-release monitoring of the families showed that the selection of habitat for relocation, based on the characterization of active burrows in the area, was successful in one of the two families relocated. One of the families abandoned the selected area for translocation during the monitoring. It is important to note that accurate population data of the targeted population, such as abundance and density, are necessary to guarantee the survival of the translocated animals in the new environment. The reintroduction method used in this study may serve as a baseline for future translocation projects for the species.

Evaluation of Antioxidant and Breast Cancer Cytotoxicity of Beta vulgaris and Daucus carota Methanol Extracts

Breast cancer is the world's most frequent malignancy among women. Many dietary antioxidants have been shown to help prevent oxidative stress, which has been linked to a variety of diseases, including cancer. Many chronic diseases, such as cancer, can be avoided by eating fruits and vegetables on a regular and balanced basis. The current study's goal is to assess the antioxidant and cytotoxic capabilities of methanolic extracts of Beta vulgaris and Daucus carota against MCF-7 human breast cancer cell lines. Therefore, the aim of the present study was to determine the phytochemical components, antioxidant capacity, total phenolic &amp; flavonoid contents in the methanolic extracts of Beta vulgaris and Daucus carota. Further, MTT assay against breast cancer cell line (MCF-7) was performed for the evaluation of cytotoxic activity. The majority of secondary metabolites were found in both the aqueous and methanolic extracts of Beta vulgaris and Daucus carota. Beta vulgaris and Daucus carota have different total phenolic and flavonoid contents. TLC results showed many spots having different Rf values. Moreover, we observe comparable cytotoxic activity in both the extracts against MCF-7 cell lines. Our results reveal that the methanolic extracts of Beta vulgaris and Daucus carota has effective phytochemical constituents, antioxidant and anticancer activity. The total phenolic and flavonoid contents vary between Beta vulgaris and Daucus carota. Further studies are needed to evaluate the chemopreventive potentials of the Beta vulgaris and Daucus carota extract when used alone or in combination with doxorubicin to mitigate the toxic side-effects of the latter.