White Cattle Research Articles

Identification of Genetic Diversity of Taro White Cattle Using Microsatellite DNA Markers

Taro white cattle have a small population found in Taro Village, Tegalalang, and Gianyar, Bali, which must be protected as one of the most valuable biological resources for the Hindu community in Bali. White cattle require conservation efforts, with morphometric characterization and genetics providing the first phases of an animal conservation program. The purpose of this study was to identify the genetic diversity of Taro white cattle based on allele frequency, heterozygosity, and fixation index (FIT) using different microsatellite DNA loci so that it can be used as a study and reference in determining policies for the conservation of Taro white cattle in Indonesia, particularly in Bali. This cross-sectional observational study utilized ETH 185, INRA 035, and INRA 037 microsatellite DNA markers. Polymerase chain reaction amplified a total of 22 Taro white cattle blood samples with an average of 4.33 alleles; the average observed heterozygosity and expected heterozygosity were 0.288 and 0.637, respectively, with an average fixation index value of 0.55. In conclusion, there is a significant deviation from the Hardy-Weinberg equilibrium with the likelihood of inbreeding, as indicated by the Hardy-Weinberg balance. The microsatellite loci used in this study can be further used to evaluate the genetic diversity of Taro white cattle.

Genetic characteristic of holstein cattle bred at Rakovskoye LLC of Primorsky Territory

The immunogenetic strucrure of Holstein cattle being imported from Germany and Hungary is determined. The presence of antigenes being met seldom – Z’ (EAA-locus), P2, T2, Q, I′, J′2, K′, P′, Y′, B′′ (EAB-locus), R1 (EAC- locus) and M (EAM-locus) is characteristic of the researched group of animals (1116 heads). Their summary frequency of being met made 17,64%. The antigenes – A2 (EAA-locus), G2, G3, Y2, E′2, E′3, G′, Q′ (EAB-locus), C1, C2, E, W, X2 (EAC-locus), F (EAF-locus), H′ (EAS-locus) are met with high frequency.They have been discovered in 40,05–98,39% of animals. Analysis of distribution of the genotypes in EAF-locus. pointed to the breach of genetic balance, that is caused by oversurplus of homozygous genotypes V/V and shortage of heterozygous F/V. Analysis of distribution of EAB-alleles exposed the high frequency of being met of the allele G2Y2Eʹ2Qʹ (q=0,2711), and also a presence in the researched group of animals the alleles typical for the black and white cattle, red steppe and other breeds of cattle. The level of homozygosity (Са) concerning EAB-locus. made 10,9%. The received materials. will be used in future for the improvement of selection and breeding work with the imported Holstein cattle.

The conjugation of productive features of dairy cattle of the Holstein breed

Relevance. Currently, in the Sverdlovsk region, more than 75% of the total number of cattle belongs to the Holstein breed, created by absorbing domestic black-and-white cattle into Holsteins. The study of the variability of the productive qualities of cows during lactation is important for identifying the possibility of increasing productive longevity and the efficiency of breeding and using Holstein cattle of the Ural selection in the conditions of the Middle Urals.Methods. The study was conducted in one of the typical livestock complexes of the Holstein black-and white cattle breed in the Sverdlovsk region. All cows that completed four lactation periods were included in the assessment. Milk productivity was assessed by control milks, milk quality indicators: MJ and MDB in milk in an average milk sample from each cow, once a month correlation coefficients between productive characteristics were calculated in the Uralplemcenter dairy laboratory. The automated program “SELEX. Dairy cattle.”Results. As a result of the conducted studies, it was found that significantly more milk was produced from age-and-sex cows during the third lactation than during the first and second, as well as the fourth lactation (p ≤ 0.01 — for the first, p ≤ 0.05 — for the second and fourth). The live weight significantly increased with age in the second lactation at p ≤ 0.01 relative to the first, and from the third onwards — at p ≤ 0.001 relative to the first lactation. There is a tendency to decrease the mass fraction of fat (MJ) in milk and a simultaneous increase in MDB with age from the first to the fourth lactation. When carrying out breeding work, it is possible to take into account the positive correlation coefficients for predicting the further milk productivity of cows according to the milk yield of the first heifers, the invariance of milk quality indicators with changes in milk yield and the positive effect of live weight of cows on their productivity. The research is exploratory and was carried out within the framework of scientific research of the Ural State Agrarian University (state registration No. AAAA19-1191014000069).

Evaluation of the productive qualities of Holstein dairy cattle

Sverdlovsk region is one of the leading regions in milk production. The main livestock of dairy cattle is represented by the Holstein breed, obtained as a result of long-term use of the global gene pool of Holstein servicing bulls. The assessment of the productive qualities of dairy cattle of the Holstein breed, including consideration of linear origin, is relevant and has practical significance. As a result of the conducted research, it was found that the farm is dominated by the number of cows of the Vis Back Ideal line, which account for more than 65.8% of the total number of cows. There is an unreliable insignificant difference in favor of cows of the Reflection Sovering line between groups of animals of different genealogical lines for the first and third lactation. With age, there is a decrease in milk quality indicators, such as FMF and PMF in milk from cows of all genealogical lines, which confirms the natural negative relationship between milk yield and milk quality indicators. The absorption of dairy black-and–white cattle of the domestic breed by the gene pool of the world’s most abundantly dairy breed, the Holstein, led to an increase in milk yields and the milk yield coefficient of these cows is much higher than 1000 kg. Despite the fact that the difference in milk yield is unreliable and the variation of the trait within each group along the line is quite significant, this is indicated by indirect traits such as the coefficient of milk production, milk yield for full-age lactation, etc., origin affects the productive qualities of cows.

Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin.

The present study reports the population structure, genetic admixture and phylogeography of cattle breeds of Sri Lanka viz. Batu Harak, Thawalam and White cattle. Moderately high level of genetic diversity was observed in all the three Sri Lankan zebu cattle breeds. Estimates of inbreeding for Thawalam and White cattle breeds were relatively high with 6.1% and 7.2% respectively. Genetic differentiation of Sri Lankan Zebu (Batu Harak and White cattle) was lowest with Red Sindhi among Indus Valley Zebu while it was lowest with Hallikar among the South Indian cattle. Global F statistics showed 6.5% differences among all the investigated Zebu cattle breeds and 1.9% differences among Sri Lankan Zebu breeds. The Sri Lankan Zebu cattle breeds showed strong genetic relationships with Hallikar cattle, an ancient breed considered to be ancestor for most of the Mysore type draught cattle breeds of South India. Genetic admixture analysis revealed high levels of breed purity in Lanka White cattle with >97% Zebu ancestry. However, significant taurine admixture was observed in Batu Harak and Thawalam cattle. Two major Zebu haplogroups, I1 and I2 were observed in Sri Lankan Zebu with the former predominating the later in all the three breeds. A total of 112 haplotypes were observed in the studied breeds, of which 50 haplotypes were found in Sri Lankan Zebu cattle. Mismatch analysis revealed unimodal distribution in all the three breeds indicating population expansion. The sum of squared deviations (SSD) and raggedness index were non-significant in both the lineages of all the three breeds except for I1 lineage of Thawalam cattle (P<0.01) and I2 lineage of Batu Harak cattle (P<0.05). The results of neutrality tests revealed negative Tajima's D values for both the lineages of Batu Harak (P>0.05) and White cattle (P>0.05) indicating an excess of low frequency polymorphisms and demographic expansion. Genetic dilution of native Zebu cattle germplasm observed in the study is a cause for concern. Hence, it is imperative that national breeding organizations consider establishing conservation units for the three native cattle breeds to maintain breed purity and initiate genetic improvement programs.

Genetic Variability in Lithuanian Black and White Cattle with Different Proportions of Holstein Bloodline

Background: Maintaining genetic variability in livestock is the important step towards developing appropriate management strategies. When considering crossing native Lithuanian Black and White cattle (BW) with Holsteins (HL), comprehensive knowledge of breed characteristics, including genetic diversity, is required. The aim of this study was to determine the genetic variability between Lithuanian Black and White and Holstein cattle breeds in relation to groups with different proportions of the Holstein bloodline. Methods: A total of 126 cattle samples were studied at 13 microsatellite loci to estimate genetic variability. Result: The largest number of alleles was detected in the BW cattle group with at least 50% Holstein blood. Private alleles were found in all cattle groups except the BW cattle group with a 83-90% Holstein bloodline. Principal coordinate analyses (PCoA) showed the separation of the HL group and other BW groups, as well as the individual that was pure-blooded Lithuanian Black and White. The results revealed further actions breeders should take to retain the required genotype of the native Lithuanian Black and White cattle breed.

Polymorphism of DNA microsatellite BMS 1248 and ILSTS 006 on taro white cattle in Bali, Indonesia

The population of Taro white cattle is only about 59 cattle. This small population which will result in the occurrence of inbreeding. This can lead to loss of genetic diversity. The purpose of this research was to characterize Taro white cattle breed using microsatellite BMS1248 and ILSTS006. A total of 20 Taro white cattle were blood drawn and extracted, and genome DNA were amplified by PCR. The result showed that the markers successfully amplified in 18 Taro white cattle. Microsatellites locus BMS1248 and ILSTS006 were shown to be highly polymorphic. In locus BMS1248 were found 5 alleles with allele’s size ranged from 139 to 165 bp. The number of alleles in locus ILSTS006 were found 5 alleles with alleles size ranged from 162 to 168 bp. The average observed and expected heterozygosity were 0.25 and 0.75 (BMS1248) and 0.5 and 0.671(ILSTS006). The PIC value was 0.679 (locus BMS1248), and 0.58 (locus ILSTS006).

ANALIZA POLIMORFIZMU W GENIE NUCB2 A CECHY PRODUKCJI MLECZNEJ BYDŁA RASY POLSKIEJ HOLSZTYŃSKO-FRYZYJSKIEJ

The aim of the study was to analyze the single nucleotide substitution polymorphism (SNP) located in exon 9 (1079 G &gt; T, rs471862956) of the gene encoding nucleobindin-2 (NUCB2) in relation to milk production traits such as milk, protein and fat yield and protein and fat content in milk. The research was carried out in a herd of Polish Holstein-Friesian black and white cattle, and genotyping of individual animals was carried out using PCR-RFLP. The most common genotype is the heterozy- gous genotype (0.53), and the more frequent allele is the T allele (0.52). The analysis of the obtained results showed that there are statistically significant relationships at the level of P ≤ 0.05 and P ≤ 0.01 between individual genotypes of the polymorphism studied and the considered milk performance char- acteristics. It was observed that individuals with the TT homozygous genotype were characterized by the highest values of such traits as: milk yield as well as fat and protein yield. However, in the case of animals of the homozygous GG genotype, it was observed that the milk obtained from them was characterized by the highest fat and protein content.

Estimation of the genetic parameters for Somatic Cell Scores in the first lactation of Romanian Black and White cattle

Abstract The aim of study was the estimation of the genetic parameters of somatic cell score of Romanian Black and White cattle population. Test-day records of somatic cell count (SCC) of 305 days lactation were from Romanian Black and White cattle population from experimental farm of our institute. The mean of somatic cell count of milk in the first lactation of Romanian Black and White cows was 291966.89. The genetic parameters were estimates with random regression test-day animal model. The heritability for somatic cell score (SCS) ranged from 0.0473 to 0.147. The breeding values for the best cows for somatic cell score were between -35.566 and -191.358. The genetic correlations between somatic cell count milk records test-day were positive. The phenotypic and genetic correlations between somatic cell count and milk yield were negative.

Relatedness of California Mastitis Test Scores and Bacteriological Plate Counts in the diagnosis of Subclinical Mastitis in white Fulani cattle from selected dairy herds

Background Subclinical mastitis remains a major challenge in the dairy industries as it affects nutritional content of milk and milk products, volume of milk production and also leads to economic loss. This study was carried out to determine the relationship between California Mastitis Test (CMT) scores and bacteria counts in the diagnosis of subclinical mastitis. A total of 339 white Fulani cattle milk samples were aseptically collected from six geographical regions of Plateau State and screened for mastitis and bacteriological analysis using the California Mastitis Test (CMT), Total Bacterial Count (TBC) and Total Coliform Count (TCC) methods respectively. Results The result of the study reveals that 31.0% of the milk samples were positive for mastitis. Based on the CMT scores, 61.9% of the samples were CMT negative, 7.1% were CMT trace while 6.5%, 11.5% and 13.0% were CMT +, CMT ++ and CMT +++ respectively. Most of the CMT negative samples (55.71%) were culture negative while almost all the CMT +++, were positive (90.91%) culture positive. A positive correlation was observed between CMT scores and plate counts (Total Bacteria count and Total Coliform Count). The Total Bacteria count ranged from 5.7x101 – 5.9x106 CFU/mL while the Total Coliform Count ranged from 0.6x101 – 3.0x105.CMT scores correlates well with bacteriological plate count of the mastitis milk samples. Conclusion There is a need for the improvement in quality and safety of dairy product because of public health risk associated with it

Relatedness of California Mastitis Test Scores and Bacteriological Plate Counts in the diagnosis of Subclinical Mastitis in white Fulani cattle from selected dairy herds

Background Subclinical mastitis remains a major challenge in the dairy industries as it affects nutritional content of milk and milk products, volume of milk production and also leads to economic loss. This study was carried out to determine the relationship between California Mastitis Test (CMT) scores and bacteria counts in the diagnosis of subclinical mastitis. A total of 339 white Fulani cattle milk samples were aseptically collected from six geographical regions of Plateau State and screened for mastitis and bacteriological analysis using the California Mastitis Test (CMT), Total Bacterial Count (TBC) and Total Coliform Count (TCC) methods respectively. Results The result of the study reveals that 31.0% of the milk samples were positive for mastitis. Based on the CMT scores, 61.9% of the samples were CMT negative, 7.1% were CMT trace while 6.5%, 11.5% and 13.0% were CMT +, CMT ++ and CMT +++ respectively. Most of the CMT negative samples (55.71%) were culture negative while almost all the CMT +++, were positive (90.91%) culture positive. A positive correlation was observed between CMT scores and plate counts (Total Bacteria count and Total Coliform Count). The Total Bacteria count ranged from 5.7x101 – 5.9x106 CFU/mL while the Total Coliform Count ranged from 0.6x101 – 3.0x105.CMT scores correlates well with bacteriological plate count of the mastitis milk samples. Conclusion There is a need for the improvement in quality and safety of dairy product because of public health risk associated with it.

Global Landscape of m6A Methylation of Differently Expressed Genes in Muscle Tissue of Liaoyu White Cattle and Simmental Cattle.

Liaoyu white cattle (LYWC) is a local breed in Liaoning Province, China. It has the advantages of grow quickly, high slaughter ratew, high meat quality and strong anti-stress ability. N6 methyladenosine (m6A) is a methylation modification of N6 position of RNA adenine, which is an important modification mechanism affecting physiological phenomena. In this study, we used the longissimus dorsi muscle of LYWC and SIMC for m6A-seq and RNA-seq high-throughput sequencing, and identified the key genes involved in muscle growth and m6A modification development by bioinformatics analysis. There were 31532 m6A peaks in the whole genome of LYWC and 47217 m6A peaks in the whole genome of SIMC. Compared with Simmental cattle group, LYWC group had 17,351 differentially expressed genes: 10,697 genes were up-regulated, 6,654 genes were down regulated, 620 differentially expressed genes were significant, while 16,731 differentially expressed genes were not significant. Among the 620 significantly differentially expressed genes, 295 genes were up-regulated and 325 genes were down regulated. In order to explore the relationship between m6A and mRNA expression in the muscles of LYWC and SIMC, the combined analysis of MeRIP-seq and RNA-seq revealed that 316 genes were m6A modified with mRNA expression. To identify differentially methylated genes related to muscle growth, four related genes were selected for quantitative verification in LYWC and SIMC. GO enrichment and KEGG analysis showed that the differentially expressed genes modified by m6A are mainly involved in skeletal muscle contraction, steroid biosynthesis process, redox process, PPAR pathway and fatty acid metabolism, and galactose metabolism. These results provide a theoretical basis for further research on the role of m6A in muscle growth and development.

Singida White cattle

This datasheet on Singida White cattle covers Identity.

Prediction of body weight from linear body measurement in two breeds of cattle

The study compared live weight and linear body measurements of two breeds of cattle (White Fulani and Muturu Cattle) and regressed linear body measurements on live weight. A total of eighty-six cattle of forty-three per a breed were reared at the cattle production unit of the Teaching and Research Farm, Akwa Ibom State University – Obio Akpa Campus, Nigeria in a semi intensive management system. Parameters measured were; live weight (LW), face length (FCL), head circumference (HC), ear length (EL), neck length(NL), neck circumference(NC), height at wither(HW), body length(BL), body circumference(BC), hind limb(HL), fore limb(FL), and tail length(TL). The data obtained were subjected to statistical analysis for morphometric traits and prediction was done through linear model regression method using SPSS statistical software package. Breed of cattle significantly (P&gt;0.05) affected all linear body measurements and live weight exception of NL .Linear body Parameters of both breeds were compared. The mean live weight of White Fulani and Muturu were 251.28kg and 149.81kg, respectively. Means of FL, EL, NL, BL, HL, TL and BC, NC and HW of White Fulani were significantly (P&lt;0.01) higher than Muturu breed but the HC of Muturu was significantly (P&lt;0.01) higher than White Fulani breed. Muturu breed had higher R2 of 0.98 in BL and BC than White Fulani breed. In conclusion, White Fulani was considered a larger breed than Muturu cattle. Live weight can be accurately predicted from linear body measurements through BC and BL in both breeds.

Single-step genomic evaluation of Russian dairy cattle using internal and external information.

Genomic data are widely used in predicting the breeding values of dairy cattle. The accuracy of genomic prediction depends on the size of the reference population and how related the candidate animals are to it. For populations with limited numbers of progeny‐tested bulls, the reference populations must include cows and data from external populations. The aim of this study was to implement state‐of‐the‐art single‐step genomic evaluations for milk and fat yield in Holstein and Russian Black & White cattle in the Leningrad region (LR, Russia), using only a limited number of genotyped animals. We complemented internal information with external pseudo‐phenotypic and genotypic data of bulls from the neighbouring Danish, Finnish and Swedish Holstein (DFS) population. Three data scenarios were used to perform single‐step GBLUP predictions in the LR dairy cattle population. The first scenario was based on the original LR reference population, which constituted 1,080 genotyped cows and 427 genotyped bulls. In the second scenario, the genotypes of 414 bulls related to the LR from the DFS population were added to the reference population. In the third scenario, LR data were further augmented with pseudo‐phenotypic data from the DFS population. The inclusion of foreign information increased the validation reliability of the milk yield by up to 30%. Suboptimal data recording practices hindered the improvement of fat yield. We confirmed that the single‐step model is suitable for populations with a low number of genotyped animals, especially when external information is integrated into the evaluations. Genomic prediction in populations with a low number of progeny‐tested bulls can be based on data from genotyped cows and on the inclusion of genotypes and pseudo‐phenotypes from the external population. This approach increased the validation reliability of the implemented single‐step model in the milk yield, but shortcomings in the LR data recording scheme prevented improvements in fat yield.

Minimal Erythema Dose Determination in Holstein Friesian Cattle.

Cattle on pasture are continuously exposed to solar UV radiation, which has been associated with biological effects such as sunburn, photosensitization, squamous cell carcinoma, and cutaneous vitamin D3 production. The minimal erythema dose (MED) required to produce first-degree sunburn (erythema) is poorly researched in cattle. Since cattle are naturally covered with dense hair coats, the MED is influenced by the UV protection offered by the hair. The objective of this study was to determine the MED on intact-hair-covered (MED-H) and shaved white skin (MED-S) of Holstein Friesian cattle. Twenty-one Holstein Friesian cows and heifers were MED tested using a narrowband UV-B LED light (peak irradiance at 292 nm) on eight hair-covered and eight shaved areas over white skin previously unexposed to direct sunlight. Erythema was visually assessed after 24 h. The mean MED-H and MED-S were 5,595 and 329 J/m2, respectively. Heifers had a higher MED-H compared to cows, 7,600 and 4,969 J/m2, respectively. The mean UV transmittance of white cattle hair was 6.7%. MED-H was correlated with hair length (Spearman's rho = 0.76). A linear regression model showed that each millimeter of hair coat length increased the MED-H by 316 J/m2. In conclusion, this study provides a MED testing protocol for cattle and reports standardized values of MED for cattle on intact-hair-covered and shaved areas.

Conservation of rare wild‐living cattle Bos taurus (L.): coat colour gene illuminates breed history, and associated reproductive anomalies have not reduced herd fertility

AbstractCoat colour patterns are important characteristics of many cattle breeds and in some are determined by a chromosomal translocation, which can have the pleiotropic effect of testicular hypoplasia. We test the hypothesis that this variant, known as the Cs29 allele, is prevalent in the ancient Chillingham White Cattle (Bos taurus) of north‐east England. Its distribution may provide insights into breed history, and as it is associated with reproductive anomalies, we investigated the issues of subfertility in this cattle herd which are of clear relevance to its conservation. We report that the cattle are homozygous for the translocation. As it is also known in other breeds of the England‐Scotland border and northern Irish area, namely the White Galloway and Irish Moiled, and in the Northern Finncattle and Swedish Mountain, we further hypothesize a Scandinavian connection for White Galloway, Irish Moiled and Chillingham cattle. We present unpublished data showing testicular hypoplasia to be present at Chillingham. Sperm quality is also known to be very low, and the question arises as to how the highly inbred (Fis = 0.92) Chillingham cattle have continued to survive in spite of these reproductive anomalies. Surprisingly, herd fertility has not declined over the last 160 years, and in the light of behavioural data, we propose this is probably because of multiple mating, there being no castration and only welfare culling in this herd. These findings have wide relevance, particularly for breeds of conservation importance and probably for other bovine species, because male subfertility, frequent in cattle generally, could restrict the choice of bulls for representation in gene banks with consequent risk of loss of lineages. Research on heterospermic insemination or multiple mating of cattle could, therefore, be a useful complement to the development of assisted reproduction technologies for cryoconservation.

Using unmanned aerial vehicles to record behavioral and physiological indicators of heat stress in cattle on feedlot and pasture

Physiological and behavioral indicators of heat stress in cattle are time- and labor-intensive to measure, and difficult to observe in extensive feedlot and pasture settings. We proposed to record respiration rate and standing behavior using unmanned aerial vehicles. Videos were recorded above steers on feedlot in the morning (0830–1130) and afternoon (1400–1700) over 10 d between 25 July and 10 August and cows on pasture over 9 d between 19 and 29 August In the feedlot, video recordings on 925 individuals (264 black coated, 413 red, and 248 white) were obtained, varying in breed which included Black Angus, Hereford, Charolais, Canadian Speckle Park, and Simmental. On pasture, video recordings on 267 individuals (116 Black Angus and 151 Hereford) were obtained. Observer software was used to analyze videos. Respiration rate in feedlot cattle was the highest in black cattle, followed by red cattle, then white cattle. Coat color did not affect respiration rate in cows on pasture; temperatures on pasture were lower than in feedlots and the effect of coat color may not manifest until a certain heat load threshold. The probability that cattle would be standing increased with heat load index in feedlot and pasture settings.

IMPLEMENTASI AJARAN TRI HITA KARANA PADA PELESTARIAN LEMBU PUTIH DI DESA TARO

Ceremony (Ritual) is the basic framework of Hinduism, which is in the form of materialistic offerings and also contains non-material elements. The ceremony is made through the concepts of Tattwa and Jnana, based on the intention of offering through the teachings of Catur Marga, namely: Karma Marga, Bakti Marga, Jnana Marga and Raja Yoga. Rituals are a procession of offerings that are organized by realizing ceremonies and events that have the aim of building morality based on morality which is implemented in ethics which will have a positive impact to get closer to God Almighty. The Hindu worship system has three dimensions, namely the application of the Tri Hita Karana Teachings. In connection with the teachings of Tri Hita Karana, the preservation of the White Ox in Taro Village is an implementation of the teachings of Tri Hita Karana itself. This study used a qualitative design. Data were collected using the observation method, interview method, library method and documentation method. Based on this analysis, the following research results were obtained: (1) The relationship between the preservation of white cattle and Parhyangan, namely: a) The relationship between preservation of white cattle and mamineh empehan in Taro Village; b) The relationship between the preservation of white cattle and the mepada ceremony in Taro Village; c) The relationship between preserving white oxen at the Tawur Panca Wali Krama Agung ceremony in Taro Village. (2) The relationship between the preservation of white cattle and Pawongan, namely: a) The relationship between the preservation of white cattle and breeders in Taro Village; b) The relationship between the preservation of white cattle and the Taro Village Community. (3) The relationship between the preservation of white cattle and Palemahan, namely: a) White cow dung is related to environmental sustainability in Taro Village; b) The relationship between the preservation of white cattle and the Tourism Park; c) The relationship between the preservation of white cattle and flora and fauna.

Protein status of holshtinized black and white cattle

We studied protein metabolism in Holsteinized black-and-white cattle. The researches were conducted in the Kuzbass at OJC «Vaganovo». Blood sample was taken from each cow, according to generally accepted of methods and analyzed in the laboratory of biochemistry from Novosibirsk State Agrarian University by using standard kits of biochemical studies on a biochemical semiautomatic analyzer Photometer 5010 V5 +. The indicators of protein metabolism were established in the blood serum of Holsteinized black-and-white cattle in average values; creatinine was characterized by the lowest phenotypic variability. The greatest positive relationship was found between albumin/globulin coefficients and albumin (r = 0.765). The reference intervals were calculated for cows of the 3rd lactation of the Holsteinized black-and-white breed with a high level of milk productivity (9-10 thousand kg): total protein 62.02-87.58 g / l; albumin 21.05-36.09 g / l; urea 2.2-4 mmol / l; creatinine 50-71 μmol / l; albumin-globulin coefficients 0.35-1.16. The data obtained can be used for assess the interior of animals.