White Cattle Research Articles

USE АDAPTOGENS TO INCREASE THE RESISTANCE AND VASSOS-RIVALINO CAPACITY OF COWS IN THE AREA OF RADIOACTIVE CONTAMINATION IN THE CONDITIONS OF KYIV POLESIE

Increased resistance to adverse factors, including the radiation – an important factor in maintaining the health and productivity of animals in remote period after the accident. Therefore it is important to develop tools and methods to enhance natural resistance and immune status of the animals kept in contaminated areas.
 At the moment not enough information on the impact of adaptogens on natural resistance and reproductive ability of farm animals in the chronic effect of small doses of radiation and especially with regard to territorial characteristics. The purpose of research was to study the possibility of using adaptogens to improve resistance and reproductive ability of cows in the area of radioactive contamination in remote period after the accident in terms of Kiev Polissya.
 Research adaptogens action on the state of natural resistance and reproductive ability of cattle in the remote period after the accident was studied in stock of dairy cows in the Agricultural production cooperative «Mriya» Ivankivsky district Kyiv region (third zone of radioactive contamination). According to the principle par-analogues was formed groups of cows Ukrainian black and white dairy cattle (62 heads). In the experiment studied radiological features welfare of cows, their overall performance and physiological state resistance.
 As used adaptogens: еleutherococcus, and trіvit tetravit.
 Efficacy was assessed by drugs hematologic indices Independence period. Monitored the course of generations, postnatal period, as the udder. To reveal hidden pathological processes conducted laboratory tests of milk sexual heat slime, postnatal excretion. Determination of radioactive contamination of water, feed conducted by generally accepted methods. Biometric data processing, obtained during the investigations carried out by M.A. Plohynskym and using the computer program Microsoft Excel 2010.
 In the Agricultural production cooperative «Mriya» Loose cows kept on deep litter of straw. Soil contamination 82,2 ± 10,14 kBq / m2. Total activity diet of 3450 Bq / day. In analyzing the morphological blood parameters of cows, found that the number of red blood cells and white blood cells in the control and experimental groups within physiological norms.
 In the leukocyte formula cows all groups, certain types of leukocytes, with the exception of young forms of neutrophils, were within physiological norms or at their lower limit. Number of young neutrophils constitute 2,0-5,3% After applying Eleutherococcus young eosinophils decreased by 50.9% tetravit - by 58.8%; tryvitu - were left unchanged. "Shift left" core neutrophils can be seen as a manifestation of lack of usefulness of the hematopoietic system. This kind of physiological miyelopoyezu irritation that is caused by a violation of neurohumoral regulations in the body.
 In the analysis of biochemical blood parameters of cows, cows significant differences in the experimental and control groups is not installed. Indicators were at the lower border of physiological norm, carotene was lower third of the index rules.
 When used Eleutherococcus Independence period was 88,3 ± 2,03, tryvitu-85,4 ± 6,20, tetravit – 55,8 ± 7,87 days. The difference between control and experimental groups was, respectively, 4.7, 24.1 and 38 days. That application tryvitu and tetravit Independence period decreased to 1-1,8 sexual cycle.
 All animals as experimental and control groups provided obstetric aid. Reproductive system disease diagnosed in all groups, the application of adaptogens their number decreased by 10-20%.
 Conclusions. The use of adaptogens positive impact on the performance of natural resistance and reproductive ability of cows.
 Based on the research we can conclude that the use of adaptogens enhances natural resistance performance, and improved reproductive capacity of animals.

INFLUENCE BODY MEASUREMENTS COWS UKRAINIAN BLACK-AND-WHITE DAIRY BREED AFTER THE FIRST CALVING ON THE FORMATION OF FURTHER MILK PRODUCTIVITI

The productivity cow dairy breed largely depends on the careful evaluation and selection of animals by milk production and the type of exterior. The estimation of animals by measuring of body plays an important role in plant-breeding work in the process of further improvement of the newly formed Ukrainian dairy breeds by the type. It’s because exterior is one of the main signs of breeding dairy cattle together with indicators of milk production, reproductive ability and productive longevity.
 The aim of research was to investigate the formation of milk productivity of cows black and white dairy cattle based on their body measurements after the first calving.
 The research was done on animals Ukrainian black-and-white dairy breed in the «Milk River» farm in the Sokal and Brody offices breeding reproducers «Breeder» Lviv region and plant-breeding «Yamnytsya» Ivano-Frankivsk region.
 The body measurements of first calving cows (height at withers and depth of chest, breast width, chest girth, scythe body length, the width of the pelvic bones and metacarpus circumference cow) and milk productivity (the milk yield, fat content in milk and milk fat) cows first, second, third
 and better lactation were studied by the materials zoo technical accounting. The force of influence on performance metrics was calculated by Single-factor disperse analysis method. The results of research were treated by variational statistics by G.F. Lakin.
 Established that the milk yield of cows Ukrainian black-and-white dairy cattle depends on body measurements after the first calving. The cows Ukrainian black-and-white dairy breed in Western Ukraine that after the first calving had a height at the withers 134-136,9 cm, depth of chest – 76 cm or more, chest width – 47 cm or more, chest girth by shoulder blades – 196-203,9 cm, scythe body length – 160-164,9 cm, the width of the pelvic bones – 53 cm and metacarpus circumference cow –19-20,4 cm, are the most productive.
 Animals with these measurements of the body most of the largest number of yield milk and milk fat had probable advantage over cows with other indicators of these measurements. By the fat
 content in milk by the first, second, third and better lactation there was no significant difference between animals with different body measurements after the first calving in most cases.
 Among the studied parameters milk productivity of cows and measurements of their bodies after first calving we found varying strength and direction of correlation. The correlation coefficients between body measurements firstborns and yield milk by the first, second, third and better lactation were positive and depended on measurements and lactation ranged from +0,297 to +0,478.
 Between such measurements of firstborns, as the height at the withers, the depth of chest, width of chest, chest girth in blades, the width of the pelvic bones and with fat content in milk for the first, second, third and better lactation correlation coefficients were though low, but positive, and dependently on measurements and lactation were within +0,026 – +0,109. The correlation coefficients between the oblique body length after the first calving cows and fat content in milk were negative (-0,045 – -0,005) and between metacarpus circumference and fat ranged from negative to positive values (-0,038 – +0,017).
 The correlation coefficients between measurements body in firstborn and milk fat yield in the first, second, third and better lactation were positive and dependently on measurements and lactation ranged from +0,288 to +0,473.
 The likely positive correlation coefficient between soundings article body and yield milk and milk fat yield indicates that selection of cows by exterior will ensure the effectiveness of selection by milk productivity.
 The impact of body measurements cows after first calving on the milk yield value dependently on lactation yield was 7,6-22,9 %, on the yield of milk fat – 7,1-22,9 %. The greatest influence on these indicators were height at the withers, the depth of the chest, chest girth shoulder blades and scythe body length, somewhat lower impact had breast width and the width of the pelvic bones and lowest – metacarpus circumference.
 The impact of body measurements cows after first calving on the fat content in milk was negligible and, dependently on measurements and lactation ranged from 0,1 to 1,6 %.

Genomics of a revived breed: Case study of the Belgian campine cattle.

Through centuries of both natural and artificial selection, a variety of local cattle populations arose with highly specific phenotypes. However, the intensification and expansion of scale in animal production systems led to the predominance of a few highly productive cattle breeds. The loss of local populations is often considered irreversible and with them specific qualities and rare variants could be lost as well. Over these last years, the interest in these local breeds has increased again leading to increasing efforts to conserve these breeds or even revive lost populations, e.g. through the use of crosses with similar breeds. However, the remaining populations are expected to contain crossbred individuals resulting from introgressions. They are likely to carry exogenous genes that affect the breed’s authenticity on a genomic level. Using the revived Campine breed as a case study, 289 individuals registered as purebreds were genotyped on the Illumina BovineSNP50. In addition, genomic information on the Illumina BovineHD and Illumina BovineSNP50 of ten breeds was available to assess the current population structure, genetic diversity, and introgression with phenotypically similar and/or historically related breeds. Introgression with Holstein and beef cattle genotypes was limited to only a few farms. While the current population shows a substantial amount of within-breed variation, the majority of genotypes can be separated from other breeds in the study, supporting the re-establishment of the Campine breed. The majority of the population is genetically close to the Deep Red (NL), Improved Red (NL) and Eastern Belgium Red and White (BE) cattle, breeds known for their historical ties to the Campine breed. This would support an open herdbook policy, thereby increasing the population size and consequently providing a more secure future for the breed.

External characteristics adults cows of ukrainian black and white dairy breed

The results of the evaluation exterior of the animals Ukrainian black and white dairy cattle in the western region of Ukraine. Studies conducted on animals Ukrainian black and white dairy cattle in the «Milk River» farm in the Sokal and Brody offices breeding reproducers «Breeder» Lviv region and plant breeding «Yamnytsya» Ivano-Frankivsk region. Exterior animals was assessed by measurement of body articles. By value measurements calculated indices of body structure cows. Weight-size factor determined by the formula D.T. Vinnychuka and P.N. Merezhko. The results of research were treated by variational statistics G.F. Lakin. Adults cows of Ukrainian black and white dairy breed for exterior fit the type of dairy animals. Measurements body cows are: height at the withers – 135,6–136,5 depth of chest – 75,1–77,1 width chest – 46,2–48,6, chest girth for by shoulder – 197,0–201,2, oblique body length – 161,4–163,6 width in clubs – 54,5–57,2 girth metacarpus – 19,1–19,5 cm. By oblique trunk length and girth metacarpus, and in the «Milk River» farm Sokal offices and plant breeding «Yamnytsya» also for girth at breast for by blades are dominated by target parameters for the desired type of animal. By other body measurements investigated cows do not reach target parameters for the desired type of animal. Further breeding work with this array of animals should be directed to increase the linear dimensions of the body, with particular attention paid to the width of the chest. Indices body structure cows in farms of different characteristic type of dairy animals. Surround dimensions of the animals constituted 494,9–501,4 cm weight-size factor – 1,08–1,15. In cows of all farms the ratio of live weight and measurements body was typical for relatively stretched animals.

Biochemical processes in cows and the biological value of milk under influence of cobalt citrate

The aim of research was to study the effect of different amounts of cobalt citrate produced by nanotechnology on biochemical processes in cows, their productivity and biological value of milk in the first two months of lactation. To achieve this goal the subsequent problems have been solved. We investigated the effect of different amounts of cobalt citrate on biochemical parameters of blood and milk of cows and controlled their average daily yield. The experiment has been conducted in 3 groups of cows (5 cows each) of Ukrainian black and white dairy cattle, analogue by body weight (590–620 kg), performance (6,5–6,8 thousand kg of milk per past lactation) and lactation (3–4 lactation). Unlike the control cows, animals experimental (ІІ) group for two months were fed by mineral supplements in the form of cobalt citrate in an amount of 19 mg Co/kg of dry matter of diet and research (ІІІ) – cobalt citrate in an amount of 34 mg Co/kg of dry matter of diet. Duration of cobalt citrate feeding of cows of research groups was 2 months. In all groups of cows the samples of venous blood were taken in preparation for feeding additives period and during 60 day of additives feeding period in order to determine the concentration of total protein, ceruloplasmin, sialic acids, hexoses bound to proteins and activity of amino transferases. The milk production has been controlled in days of sampling with the definition of daily milk yield per cow and taking an average sample for the determination of milk fat, protein, lactose, inorganic phosphorus and calcium. It has been established that the inclusion in the diet of cows of experimental (ІІ) group of mineral supplements contributed to an increase in animal blood total protein content by 11.9% (P < 0.05), inorganic phosphorus by 15.3% (P < 0.05) and hexoses bound protein by 8.8% (P < 0.05). Under these conditions milk content of inorganic phosphorus increased by 5.6% (P < 0.05) and average daily milk production increased by 4.5%. The use of mineral additives in third experimental group of animals within two months contributed to an increase in blood ceruloplasmin content by 5.1% (P < 0.05) and hexoses bound protein by 11.5% (P < 0.01). Mineral supplements promote 5.4% raise of average daily milk production and increase it to the lactose content by 0.10% (in absolute).

Adaptation measures practiced by the farmers rearing dairy animals at farm level to mitigate the influence of climate change

Climate change has an adverse effect on climate sensitive livestock sector. A well-structured interview schedule was used to collect the practices adopted by the livestock farmers at farm-level in irrigated and rainfed areas of Namakkal district in Tamil Nadu. Practices adapted to mitigate the effects of climate change were documented from 320 livestock farmers, 160 from irrigated and 160 from rainfed areas. Providing shade during day time was the major practice adapted in housing, cooling animals by washing, alteration in the grazing time, cooling and not allowing for grazing after insemination, deworming animals two to three days prior to insemination, feeding lemon after insemination, feeding mineral mixture during summer, soaking concentrate feed in water, feeding aloevera and neem oil for 15 days before insemination and shifting from buffalo to white cattle were the practices adapted by the livestock farmers to mitigate the effect of climate change. Further, the field experts opined that feeding mineral mixture (100%), alteration in grazing time (100%), cooling animals after insemination (97.22%), deworming of animals two or three days prior to insemination (91.67%) had effect at field level. Field experts suggested that feeding lemon after insemination, feeding mineral mixture during summer and feeding aloevera and neem oil for 15 days before insemination were the practices need further scientific research for up-scaling of practices.

Удосконалення української чорно-рябої молочної породи в умовах Тернопільської області у процесі консолідації стад та формування бажаного молочного типу корів

Удосконалення української чорно-рябої молочної породи в умовах Тернопільської області у процесі консолідації стад та формування бажаного молочного типу корів

Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

Dynamic of growth of live weight and exterior–constitutional peculiarities cows ukrainian black and white dairy cattle different types of constitution

Compound part of complex information approach towards desired animals determination is elaboration, approbation and extensive utilisation of different projection methods in selection and breeding work that is based on animals' body constitutional peculiarities. The analysis of the dynamics of growth of live weight and exterior features–constitutional was performed for cows Ukrainian black and white dairy cattle breeding farm for various types of constitution «Yamnytsya» Tysmenytsya district of Ivano–Frankivsk region. Determining the type of constitution animals was carried out by mass–metric coefficient (on D. T. Vinnychukom with co–authors): dense, intermediate and loose. Found that animals of different types of constitutions differ in growth dynamics live weight of heifers and exterior features–constitutional cows firstborn. Animals dense type of constitution by live weight during their growing intermediate and superior animals loose type of constitution as at birth and at age 6, 12 and 18 months and the first insemination. The highest intensity of live weight growth during the growing animals were observed dense type of constitution. During the period from birth to 18 months of age daily gain in animals these groups was 709.3 g, which is significantly higher than the loose types in 60.3 g (P < 0,001). According to body weight and body core soundings cows firstborn different types of constitutions for most indicators have preferred dense animal–type constitution. They noted the good development of the chest, a relatively deep but not broad chest, which are typical for dairy cattle. Probable animals advantage dense type of interim constitution and loose observed both in live weight and in many soundings and indices of body structure.

Assessment breeding high–yielding cows bukovynske factory type of ukrainian red–white dairy cattle

The results of research on the assessment of breeding qualities of high performance cows Bukovina Ukrainian plant type red–white dairy breed. Established that the productive potential of the cow estimated high enough that opens significant opportunities for formation buhayvidtvoryuvalnoyi group of cows to produce the next generation of producers. Much of cows good milking combines high content of fat and protein in milk. The best indicators for milk yield (over 7000 kg) were daughters of sires Captain 6775, Artek 344, Polar 49, Mozart 475, Dzharomira 6296217, Guido 40535, Madzhesti 2071114. For fat milk probably stood daughter Nord 3126 (3.86%) and was the lowest rate in cows that received from the Senate in 1632, 3160 and Lighthouse Dzharomira 6296217 (3.75%). The content of protein in milk was the best daughter of Captain 6775, Artek 344, Polar 49, 475 Mozart (3.31 – 3.33%) and slightly lower figure was Dzharomira daughters 6296217 (3.25%). Note that the daughter of Captain bulls 6775, the Arctic 49, Nord 3126 well combine high milk production of fat and protein in milk. It is appropriate to further use of highly productive cows, bulls–sires and their descendants in the system of selection and breeding herds in the creation of high–performance factory type Bukovina Ukrainian red–white dairy breed for specific economic conditions and effective improvement genealogical structure

The Influence of Age, Sex and Breed on the Susceptibility to Trypanosomiasis in Cattle

A survey was conducted in Song Local Government Area of Adamawa State on Trypanosomiasis to determine the influence of age, sex and breed on the susceptibility to the infection. A total of 240 blood samples of different breeds, Sexes and ages of cattle were collected from randomly selected herd and analyzed in laboratory using wet, thick, thin smear and Buffy coat concentration techniques. An overall infection rate in males and females were 9.17% and 17.50% respectively, infection rates in Red Bororo, Adamawa Gudali and White Fulani were found to be 13.16%, 14.00% and 12.00% respectively. The result shows that Adamawa gudali has the highiest with White Fulani cattle having the lowest rate of infectioon. Trjpanosoma vivax infection was 9.17% and Tiypanosoma congolence was 4.16%. This result shows that the trypanosomiasis in the area is of great economic importance.Key words: Trypanosomiasis, age, sex, breed.

215 PRODUCTION OF PROGENY WITH SEXED WHITE HANWOO (KOREAN NATIVE ALBINO CATTLE) SEMEN

The aim of the study was to produce sexed progeny of the genetically confined White Hanwoo (albinism). A White Hanwoo bull was selected as the semen donor. The X-bearing sperm were sorted by MoFlo XDP cell sorter from Korea Sexing Biotech (Daegu, South Korea). To compare pregnancy and birth rates as well as semen quality, a Korean proven bull (KPN) was used as control. Total number of nonsorted sperm was 20 × 106 per straw. Double insemination of 2 or 4 × 106 sexed and frozen X-bearing sperm were performed on Hanwoo heifers. The abnormality of nonsorted albino spermatozoa (5 ejaculates) was 24.9 ± 7.31% (but 18.7 ± 6.2% C in KPN 768, derived from 5 straws) with a higher number of distal reflex abnormality in midpiece (11.7% compared with that of KPN 768, 5.6%). There were no differences in pregnancy and birth rates when 2 or 4 × 106 X-bearing sperm were inseminated but nonsorted semen had higher pregnancy rates (P < 0.05). The pregnancy rates of KPN 768, 2 × 106 cells, and 4 × 106 cells X-bearing sperm of White Hanwoo cattle were 85.0% (17/20), 26.3% (5/19), and 50% (7/14), respectively. The birth rates were 80.0% (16/20), 15.8% (3/19), and 21.4% (3/14), respectively. The female offspring rates were 43.8% (7/16), 100% (3/3), and 100% (3/3) (P < 0.05). These results indicated that sex-sorted albino Hanwoo semen could be used for the production of wanted progeny with 2 × 106 cells/straw for AI to heifers.

COWS AND GOATS AS SENTINELS FOR ENVIRONMENTAL LEAD (Pb) BURDEN IN ZARIA, NIGERIA

Blood lead levels (BLLs) is considered as a good indicator of current body burden of the toxic metal. Assessment of BLLs of white Fulani cattle(Bos primigenius indicus)and red Sokoto goats (Capra aegagrus hircus) grazed freely on open pastures in Zaria was carried out by Atomic Absorption Spectrophotometry using Shimadzu Atomic Absorption Spectrophotometer (model AA-6800, Japan) after wet digestion to evaluate the potential effects of the metal on the animals, the toxicological implication of consuming this major source of animal protein by humans and most importantly as an indirect measure of the lead pollution status of Zaria ecological geochemical environment. The blood lead levels of Bos primigenius indicus ranged from a minimum of 0.62 mg/l to a maximum of 2.28 mg/l with a mean value of 1.36±0.48 mg/l. On the other hand, blood lead levels of Capra aegagrus hircus ranged between 0.26 mg/l and 1.56 mg/l with a mean value of 0.89±0.43mg/l. The BLLs of both cows and goats were found to exceed the allowable limit for the elements in blood of food animals indicating that cows and goats grazed freely on open pastures in Zaria metropolis are not safe for human consumption. Routine monitoring of the heavy metal in plant and animal products is therefore recommended to safeguard human health. Most importantly, the study indicates the presence of elevated levels of environmental lead in Zaria ecological geochemical environment.

Microsatellite and Mitochondrial DNA Study of Native Eastern European Cattle Populations: The Case of the Romanian Grey.

The Eastern European Grey cattle are regarded as the direct descendants of the aurochs (Bos taurus primigenius). Nowadays in Romania, less than 100 Grey animals are being reared and included in the national gene reserve. We examined the genetic diversity among Romanian Grey, Brown, Spotted and Black and White cattle breeds, with a particular focus on Romanian Grey through the use of (i) 11 bovine specific microsatellite markers on 83 animals and (ii) 638 bp length of mitochondrial DNA (mtDNA) D-loop region sequence data from a total of 81 animals. Both microsatellite and mtDNA analysis revealed a high level of genetic variation in the studied breeds. In Romanian Grey a total of 100 alleles were found, the mean number of observed alleles per locus was 9.091; the average observed heterozygosity was 0.940; the Wright’s fixation index (FIS) was negative (-0.189) and indicates that there is no inbreeding and no selection pressure. MtDNA analysis revealed 52 haplotypes with 67 variable sites among the Romanian cattle breeds without any insertion or deletion. Haplotype diversity was 0.980 ± 0.007 and ranged from 0.883 ± 0.056 (Brown) to 0.990 ± 0.028 (Spotted and Black and White). The highest genetic variability of the mtDNA was recorded in the Grey breed, where 18 haplotypes were identified. The most frequent mtDNA D-loop region belonged to T3 haplogroup (80.247%), which was found across all studied breeds, while T2 haplotypes (16.049%) was only found in Grey, Spotted and Black and White genotypes. The T1 haplotypes (3.704%) were found in the Grey and Spotted. The current results contribute to the general knowledge on genetic diversity found in Eastern European cattle breeds and could prove a valuable tool for the conservation efforts of animal genetic resources (FAnGR).

Antimicrobial susceptibility of commensal Escherichia coli from faeces of apparently healthy white fulani cattle (Bos indicus)

Abstract Antimicrobial susceptibility of commensal Escherichia coli (E. coli) from faecal samples of white Fulani cattle (Bos indicus) was determined in Anyigba, Kogi state. Sixty (60) faecal samples were

Amounts of essential and non-essential amino acids and the ratio in Lithuania bred cattle meat

The aim of the research was to determine the amounts of essential and non-essential amino acids and the ratio in various breed cattle meat. The content of amino acids and the ratio at the longest dorsal muscle (musculus longissimus dorsi) was determined analyzing Angus, Hereford, Charolais, Limousine purebreds, Lithuanian Black and White x Charolais (LTBWxCHA) crossbreed, Lithuanian Black and White x Limousine (LTBWxLI) crossbreed, Lithuanian Black and White x Simmental (LTBWxSI) crossbreed and Lithuanian Red x Limousine (LTRxLI) crossbreed. Analyzing the research results it was noticed that Lithuanian Black and White cattle breed meat contained the highest total amount of amino acids and Angus breed cattle meat contained the smallest amounts. The difference amounted to 33.87 g/kg or 4.1 percent (p<0.01). While comparing total amounts of amino acids at the purebred cattle breeds and the crossbreeds, it was noticed that the meat of purebred cattle contained higher amounts of amino acids, the difference ranging between 18.54 and 19.00 g/kg or 2.23-2.28 percent (p<0.01). Purebred cattle meat contains higher amounts of essential amino acids compared to crossbreed cattle meat. The meat of Aubrac and Angus breeds was determined to have the highest meat protein sufficiency rate. The lowest biological values were determined at Lithuanian Black and White x Charolais crossbreed meat. The highest amount of the amino acid leucine was observed in Aubrac breed cattle meat (p<0.05). The difference of the leucine amounts reached up to 0.45 g/kg (p<0.01) comparing to various crossbreed cattle meat.

Research and Application of the 3D Mathematic Model of Ore-Body Based on Suppac Software - Yunnan White Cattle Factory Silver Polymetallic Mine

Three-dimensional models of deposit are the foundation of realizing digital mineral deposit , with the aid of mining software Surpac,it can establish deposit database, the three dimensional model of the mining surface,,orebody and roadway of the yunnan white cow factory silver polymetallic deposit.The 3D model can show the vivid image of the mining topography and geomorphology, ore body position, gallery situation. The ore model of orebody grade founding by kriging can show the distribution change of orebody grade clearly, and finally estimate the amount of ore body. Comparing with the traditional estimation method of exploration report, we can find the estimation of mineral resources by software is accurate.

Detection of Cocaine and Amphetamine Regulated Transcript in the abomasum of slaughtered bulls with different daily body weight gains

Despite numerous published studies, the relationship between the amount of secreted cocaine and amphetamine regulated transcript and the daily body weight gain has still not been well explained. The aim of this study was to determine the incidence of cocaine and amphetamine regulated transcript in the outlet wall of the abomasum of bulls with different daily weight gains. The study was performed on 15 bulls, breed crosses of local black and white milk cattle and Limousin bulls. The animals were slaughtered at the mean age of 543–549 days and body weight of 441.0–491.4 kg. Fragments of the outlet wall of the abomasum were sampled for analyses during routine slaughter. Immunohistochemical assays showed that slowly growing bulls (803 g/day) had significantly (P ≤ 0.05) fewer positive structures of cocaine and amphetamine regulated transcript (by 1.65 × on average) compared to bulls with large daily weight gains (905 g/day). This tendency was also observed in the case of cocaine and amphetamine regulated transcript distribution in particular layers of the abomasum wall. The most numerous positive structures of cocaine and amphetamine regulated transcript were found in the nerve fibres of the muscularis and in the muscle plexus, whereas they were evidently less numerous in the submucous plexus. Our results suggest that the number of cocaine and amphetamine regulated transcript immunopositive structures is associated with the growth intensity of the animals, and frequent occurrence of this neuropeptide in the nerve fibres and the muscular plexus proves its role in the control of stomach emptying.

Assessment of the effects of different concentrations of ethylenediaminetetraacetic acid (EDTA) on erythrocytic indices of Nigerian White Fulani cattle and West African Dwarf goat

The aim of the study was to evaluate the effects of varying concentrations of ethylenediaminetetraacetic acid (EDTA) on blood samples from White Fulani breed of cattle and West African Dwarf goat from Nigeria. Sample sizes of 20 animals were used for both species. Different concentrations of EDTA (2, 4, 8 and 16 mg/ml) were used. The packed cell volume (PCV), red blood cell (RBC) and haemoglobin (Hb) concentration of blood samples collected from White Fulani breed of cattle and West African Dwarf goat into bottles containing 16 mg/ml of EDTA were significantly lower (P < 0.05) than those samples collected from the same animals into bottle containing 2 mg/ml (control). Similarly, the PCV, RBC and Hb values of the West African Dwarf goats in bottles containing 8 mg/ml of EDTA were significantly lower than those of the samples in the control (2 mg/ml). This study has shown that high concentration of EDTA as an anticoagulant can lead to a false erythrocytic index especially the PCV. In collecting blood samples for evaluation of haematological parameters, therefore, the blood volume/anticoagulant ratio must be strictly adhered to prevent error in the evaluated parameters in cattle and goats. Taken together, there is tendency for haemolytic anaemia to occur in blood sampled at higher concentration of anticoagulants in West African Dwarf goat than in White Fulani breed of cattle.

Association between milk protein polymorphism and milk production traits in Black and White dairy cattle in Turkey

Milk protein polymorphism such as as1-casein (as1-Cn), b-lactoglobulin (b-Lg), b-casein (b-Cn) and k-casein (k-Cn) of Black and White dairy cattle were investigated in this study. The genetic structure of herd was examined for these loci. At the sametime the relationships between milk protein types and some milk production traits were determined. Milk production traits were chosen as milk yield, average daily milk yield and lactation length. These traits were associated with milk protein types. Genetic variants of milk protein were identified by starch gel electrophoresis containing mercaptoethanol and urea. The allele gene frequencies of A, B, and C of as1-Cn loci and A, B of b-Cn were found to be 0.01; 0.97; 0.02 and 0.95; 0.05 respectively. Furthermore, the allele frequencies of A, B of k-Cn and A, B of b-Lg were found to be 0.68; 0.32 and 0.55; 0.45 respectively. For as1-Cn BB phenotype was found to have higher milk yield than those of as1-Cn AB and as1-Cn BC phenotypes, and b-Lg AA phenotype was detected to have higher milk yield than those of b-Lg AB and b-Lg BB types but were not statistically important. It was concluded that the genetic structure of the four loci were determined in Black and White cattle, but there was no significant association between the milk protein types and milk production traits in the study. Key words : Milk protein polymorphism, casein, lactoglobulin, Black and White dairy cattle.