Development Of Wheat Seedlings Research Articles

Analysis of short-term treatment effects of dielectric barrier discharge plasma to improve germination characteristics of wheat seeds

In this work, an effort has been made to show that a cold atmospheric pressure plasma (CAPP) can be applied to promote the growth and development of wheat seedlings. A dielectric barrier discharge (DBD) plasma-based reactor has been developed having two-roller conveyor rollers running on sprockets to generate cold plasma. Wheat seeds (Triticum aestivum) are treated with cold plasma for shorter durations to examine their germination parameters. In the cold plasma, reactive oxygen and nitrogen species (RONS) are generated which alter the surface of the seeds. This results in enhanced germination, growth, and enduring vitality of the plants enhance seed germination. After plasma treatment, the germination rate was enhanced from 84% to 98%. Also, after treatment surfaces of wheat seeds became more hydrophilic, as confirmed by a decrease in the water contact angle which results in higher seed germination. It is inferred that plasma is a viable alternative to the conventional pre-sowing seed treatment for germination due to its uniform treatment without degradation and the use of pesticides.

TaGSK3 regulates wheat development and stress adaptation through BR-dependent and BR-independent pathways.

The GSK3/SHAGGY-like kinase plays critical roles in plant development and response to stress, but its specific function remains largely unknown in wheat (Triticum aestivum L.). In this study, we investigated the function of TaGSK3, a GSK3/SHAGGY-like kinase, in wheat development and response to stress. Our findings demonstrated that TaGSK3 mutants had significant effects on wheat seedling development and brassinosteroid (BR) signalling. Quadruple and quintuple mutants showed amplified BR signalling, promoting seedling development, while a sextuple mutant displayed severe developmental defects but still responded to exogenous BR signals, indicating redundancy and non-BR-related functions of TaGSK3. A gain-of-function mutation in TaGSK3-3D disrupted BR signalling, resulting in compact and dwarf plant architecture. Notably, this mutation conferred significant drought and heat stress resistance of wheat, and enhanced heat tolerance independent of BR signalling, unlike knock-down mutants. Further research revealed that this mutation maintains a higher relative water content by regulating stomatal-mediated water loss and maintains a lower ROS level to reduces cell damage, enabling better growth under stress. Our study provides comprehensive insights into the role of TaGSK3 in wheat development, stress response, and BR signal transduction, offering potential for modifying TaGSK3 to improve agronomic traits and enhance stress resistance in wheat.

Efficacy of Plant Growth Promoting Rhizobacteria to Improve the Biochemical Properties and Development of Wheat Seedlings under Drought Stress Control

The most significant cereal crop is wheat, which ranks third among the world's most demanding staple crops after rice and maize. Temperate climates with moderate rainfall are ideal for growing it. Under the current changing climate, abiotic stress conditions, particularly drought conditions, have a significant impact on wheat crop productivity. Plants under drought stress have less water accessible to them, which slows down germination, growth, and yield. In this study, plant growth promoting rhizobacteria (PGPR) is used to boost the drought tolerance capability of the wheat plant. PGPR isolate KD-3B strain is used to investigate the plant growth characteristic such as, mineral solubilization (zinc, phosphate, and potassium), ammonia production, indole acetic acid (IAA), and the enzyme 1-carboxylic acid (ACC). Moreover, the plant showed drought tolerance at polyethylene glycol (PEG 6000) concentrations of 0%, 5%, 10%, 15%, 20%, and 25% (w/v). Wheat seedlings were exposed to both normal and drought conditions when they interacted with the KD-3B bacterial strain, which possesses many features that promote plant development. In comparison to growth in a non-PEG condition, this strain is tested for relative growth up to 41.05% in a 25% PEG concentration. When inoculum of KD-3B bacterial strain used under normal and drought conditions, the relationship between microbes and plants was examine in wheat seedlings grown under hydroponic condition. Under drought stress condition significant changes were observed in the morphological and biochemical conditions of seedlings. This research concluded that, in drought stress condition, PGPR KD-3B strain inoculated seedlings showed increased chlorophyll content, TSS content, malondialdehyde concentration and decreased proline, H2O2 content, SOD and POD activities, as compared to the negative control seedlings. The PGPR stain KD-3B improved their biochemical and physiological parameters and plant microbe interaction decreased the deleterious effect of drought on wheat seedlings.

The influence of antagonistic microorganisms on limiting the development of Fusarium fungi and the development of winter wheat seedlings

The study aimed to assess the impact of Trichoderma harzianum, Trichoderma atroviride, Sarocladium strictum, Wickerhamomyces anomalus and Bacillus spp. on the growth inhibition of Fusarium spp. colonies, and on the health and development of winter wheat va¬rietas Euforia seedlings. In the laboratory conditions, the presence of T. atroviride and T. harzianum the most strongly limited the growth of the colonies of Fusarium culmorum, Fusarium graminearum and Fusarium avenaceum. Well-developed growth inhibition zones were observed in the bicultures containing strains of Bacillus spp. and W. anomalus. The development of wheat seedlings in the presence of antagonistic and pathogenic fungi was varied, but W. anomalus provided the best protection against damping off caused by F. culmorum and F. graminearum. The influence of individual antagonistic microorganisms in variants with inoculation with pathogenic microorgan¬isms on the green mass of wheat seedlings was variable. The best results were achieved with T. atroviride, S. strictum and Bacillus spp.

Analysis of rhizosphere fungal community of agricultural crops cultivated in laboratory experiments on Chernevaya taiga soil.

Chernevaya taiga of Western Siberia, Russia, is a unique ecosystem characterized by fertile soil, exceptionally large herbaceous plant sizes, and extraordinarily rapid rates of plant residue degradation. We expected that growing crops on soil collected from Chernevaya taiga, which has never been used for agricultural purposes before, would result in a distinct rhizospheric fungal community. This community could potentially yield novel, potent biostimulators and biocontrol fungi for modern agriculture. To check this idea, we used high-throughput ITS sequencing to examine the microbial communities in the rhizosphere of spring wheat and radish grown in greenhouse experiments on Chernevaya and control soils. Additionally, representative fungal strains were isolated and assessed for their ability to promote growth in wheat seedlings. The study revealed that the most abundant phyla in the rhizospheric fungal community were Mortierellomycota, primarily consisting of Mortierella species, and Ascomycota. Mucor and Umbelopsis comprised the majority of Mucoromycota in the control soils. Fusarium and Oidiodendron, two potentially plant-pathogenic fungi, were only found in the rhizosphere of crops grown in the control soil. Conversely, Chernevaya soil contained a diverse range of potential biocontrol fungi for plants. Tested novel fungal isolates showed a stimulating effect on the development of wheat seedlings and positively affected their rate of biomass accumulation. The results of the study demonstrate that the soil of Chernevaya taiga do indeed contain fungi with prominent potential to stimulate agricultural plants growth.

Remediation of wastewater by biosynthesized manganese oxide nanoparticles and its effects on development of wheat seedlings.

Nanoparticles play a vital role in environmental remediation on aglobal scale. In recent years, there has been an increasing demand to utilizenanoparticles in wastewater treatment due to their remarkable physiochemical properties. In the current study, manganese oxide nanoparticles (MnO-NPs) were synthesized from the Bacillus flexus strain and characterized by UV/Vis spectroscopy, X-ray diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy. The objective of this study was to evaluate the potential of biosynthesized MnO-NPs to treat wastewater. Results showed the photocatalytic degradation and adsorption potential of MnO-NPs for chemical oxygen demand, sulfate, and phosphate were 79%, 64%, and 64.5%, respectively, depicting the potential of MnO-NPs to effectively reduce pollutants in wastewater. The treated wastewater was further utilized for the cultivation of wheat seedlings through a pot experiment. It was observed that the application of treated wastewater showed a significant increase in growth, physiological, and antioxidant attributes. However, the application of treated wastewater led to a significant decrease in oxidative stress by 40%. It can be concluded that the application of MnO-NPs is a promising choice to treat wastewater as it has the potential to enhance the growth, physiological, and antioxidant activities of wheat seedlings.

Combined Triazole Disinfectants and Their Influence on the Growth and Development of Spring Wheat Seedlings

Seed treatment with fungicides is a crucial link in the plant protection system, and systemic drugs give the best effect. Therefore, it is very important to develop scientific foundations for the preparation of complex preparations with a low rate of consumption of the active substance and the inclusion of plant growth regulators that enhance growth processes and the yield of the main crop. In recent years, the directions of modification of known and practiced fungicides by various methods, including methods of mechanochemistry, have been promising. The problem of creating both new substances and their effective formulations remains urgent. Taking into account the prospects and high efficiency of triazole fungicides in the system of complex plant protection, the creation of multicomponent seed protectants of grain crops is proposed. The purpose of this study was to develop preparations based on tebuconazole (TBC) and propiconazole (PPC) in the form of solid dispersions (SD) and to study their biological activity on spring wheat plants in the conditions of the Trans-Urals. At the same time, the retardant effect of these triazoles was also studied with the help of the domestic growth regulator Floroxan. The use of new preparations on spring wheat made it possible to reduce the damage to the culture by root rot by 85–87%, productive bushiness increased by 6–11% compared to the control without treatment. A reliable increase in crop yield of 2.2 c/ha or 14% was obtained using preparation 2 (TD of the composition TBC : PPC : AG : silica = 1 : 1 : 7 : 1 + Phloroxane when the polysaccharide (AG) and the auxiliary substance of silica are included in the composition). The inclusion of the growth regulator Floroxan in the composition of the mordant mixture reduced the antioxidant effect of tebuconazole and stimulated the growth and development of wheat seedlings. It was proved that such a biological effect was achieved in conditions of drought of varying intensity during the growing season of 2021–2022.

Root Exudates of Fifteen Common Weed Species: Phytochemical Screening and Allelopathic Effects on T. aestivum L.

Through allelopathic interactions, plants may either suppress competing species or promote those that may help them better adapt to their environment. The purpose of this research was to determine how the root exudates of 15 common weeds affected the germination of wheat (Triticum aestivum L.). Every other day, 15 seeds were dispersed over Petri dishes (with filter paper) and pots (with garden soil) and treated with 1 mL and 5 mL of aqueous root exudates, respectively. Distilled water had the highest germination rate at 86.33% and the lowest at 64.00% (p = 0.001) for Commelina benghalensis in the Petri dish containing root exudates. The seed germination percentage of the pot condition was the lowest, at 68.45% (p = 0.004), for Solanum nigrum, while the control was 87.23%. Similarly, the lowest shoot length, 12.01 cm (p = 0.0025) in Mikania micrantha, and the lowest root length of 2.17 cm (p = 0.0048) in Leucas aspera, were recorded, whereas the control was 19.13 cm and 3.46 cm, respectively, in Petri dishes. In addition, the lowest shoot and root growth were 9.72 cm (p = 0.0004) in Mikania micrantha and 4.34 cm (p = 0.0019) in Spilanthes acmella, while the control was at 20.13 cm and 6.42 cm, respectively, for pot culture. Furthermore, in seedlings, biomass studies of treated T. aestivum showed elevated malonaldehyde (MDA) levels in both Petri dishes and pot cultures. However, chlorophyll a and b levels, as well as those of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), are lower than in the control group. Both enzymes were elevated in pot cultures compared to those grown in Petri plates. The dry weight of T. aestivum seedlings cultivated in pots and Petri dishes demonstrated its considerable allelopathic influence. This research found that the root exudates of all the weeds studied could have the capacity to impede wheat seed germination as well as the development of wheat seedlings. These inhibitory effects were higher in Petri dishes than in pot cultures.

Study on the Effect of Sodium Nano Lignin Sulfonate on the Growth and Development of Wheat Seedlings

Study on the Effect of Sodium Nano Lignin Sulfonate on the Growth and Development of Wheat Seedlings

Effect of vermitea on morphological parameters of wheat seedlings

It was shown that low concentrations of vermitea stimulated the development of wheat seedlings under water culture conditions. Both shoot length and root length were statistically significantly longer in the vermitea nutrient solution than in the control (Hewitt’s solution). A very strong positive correlation (r=0.99) was found between the content of humic acids in hydroponic solutions and the length of roots formed by wheat seedlings.

Applying of Plants to Regulate Mycobiota of Winter Wheat Seeds

Plants, having a wide array of different substances that are natural to ecosystems, can be effective biofungicides. Most scientific studies have demonstrated the effectiveness of plant extracts against certain phytopathogenic species/genera in vitro. These results do not provide an answer to how certain substances might affect the systems of microorganisms. The aim of the work was to study the influence of plants on isolation of mycobiota representatives of winter wheat seeds, the peculiarities of its germination and seedling development. The mycocomplex of the seeds of the 2017 and 2019 harvests from the North-East of Ukraine was studied. The seeds were kept in aqueous plant solutions and spread on potato-glucose agar. The effects of Citrus sinensis L., Citrus limon L., Zingiber officinale Roscoe, Larix decidua Mill., and Pinus sylvestris L. were evaluated. All plant solutions changed the quantitative and qualitative composition of seed mycobiota. The negative effect of soaking seeds on the development of wheat seedlings was established, which will not allow them to be used to regulate the seed mycocomplex in the future. C. limon (67%) and Z. officinale (on average 52%) most effectively reduced the number of dominant Alternaria sp. Seed treatment increased the number of fungal colonies compared to the control, except for the 40% ginger solution, and when ginger was used, the spectrum of fungi was expanded, and when citrus and pine were used, it was narrowed. Plant solutions changed the dominance of Alternaria sp. in the seed mycobiota on the prevalence of Penicillium sp. and Aureobasidium pullulans (de Bary) G. Arnaud. The mycobiota of wheat seeds acquired the most significant changes under the influence of L. decidua and P. sylvestris

Morphology and Nitrogen Uptake and Distribution of Wheat Plants as Influenced by Applying Remedial Urea Prior to or Post Low-Temperature Stress at Seedling Stage

Wheat production is dramatically influenced by temperature. Low-temperature stress that frequently occurs seriously hampers the growth and development of wheat seedlings. In order to alleviate the damage of low temperature to wheat plant growth, remedial nitrogen was applied prior to or post low-temperature stress at seedling stage using controlled-temperature incubators to explore the difference in wheat morphology and nitrogen absorption and utilization efficiency. Nitrogen amendment significantly increased the leaf area, seedling height, tiller number and dry matter weight of wheat plants as compared with the treatment without nitrogen amendment under temperature stress. Remedial nitrogen applied prior to low-temperature stress (N-LT) was more conducive to increase the above parameters than that applied post low-temperature stress (LT-N). In addition, N-LT enhanced the ability of roots to absorb remedial 15N urea, promoted the growth and development of wheat plants under low-temperature stress, resulting in higher leaf SPAD readings, more new tillers, as well as increased dry weight of roots and above-ground organs. This study suggested that remedial nitrogen should be applied prior to low-temperature stress because it is more effective in restoring the growth of wheat plants under low-temperature stress.

Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10-30) on wheat seedling development

Objective: Performing a study on a wheat growth bio assay with a homeopathic dilution of gibberellic acid at different seasons of the year.
 Methods: Grains of winter wheat (Triticum aestivum, Capo variety) were observed under the influence of extremely diluted gibberellic acid (10-30, 30x). Analogously prepared water was used for control. 15 experiments were performed, 9 in autumn season (5 researchers, 4,440 grains per group), and 6 in winter / spring (4 researchers, with 3,140 grains per group).
 Results: All 9 autumn experiments showed less stalk growth in the verum group (p > 0.01 in 4 cases, p > 0.05 in 3, trend in 2 cases). Mean stalk lengths (mm) were 46.97 + 20.50 for verum and 50.66 + 19.77 for control at grain level (N = 4,440 per group) and + 3.87 and + 3.38 respectively at dish level (217 cohorts of 20 or 25 grains per treatment group). Verum stalk length (92.72%) was 7.28% smaller than control stalk length (100%). In contrast, no reliable effect was found in experiments performed in winter / spring (less stalk growth in 1 case, no difference in 1, more growth in 3 cases). Overall verum stalk length (103.64%) was 3.64% slightly greater than control stalk length (100%). Data were found to be homogeneous within the control groups as well as within the verum groups.
 Conclusion: Results suggest that especially in the experiments performed in autumn, there was an influence of gibberellic acid 30x on wheat seedling development. The effect size is small when calculation is done on the basis of grains (d = 0.18) but high when done on the basis of dishes (d = 1.02). In contrast, no reliable effect was found in experiments performed in winter / spring. Further experiments should thus be performed in the autumn season.

Impacts of cuprous oxide nanoparticles on wheat root morphology and genotoxicity.

To explore the ecotoxicity of Cu2O nanoparticles (NPs) on plant roots, the effects of Cu2O-NPs with different concentrations of 10, 50, 100 and 200 mg·L-1 on the seedling growth, root morphology, and cytogenetic toxicity of wheat 'Zhoumai 18' (Triticum aestivum Zhoumai 18) were examined in a hydroponic experiment. The results showed that Cu2O-NPs inhibited the growth of wheat seedlings. Cu2O-NPs reduced root and shoot lengths, fresh weights of shoot and root, root relative activity and ratio of root to shoot of wheat seedlings, but increased primary root number. Furthermore, with the increases of Cu2O-NPs concentrations, the root elongation zone shortened and the root became hard and brittle, while the average diameter of roots increased. Under the concentration of 100 mg·L-1 Cu2O-NPs, the mitotic index significantly decreased, and vacuolization, plasma membrane detachment, chromosomal abnormality occurred in the root tip cell. In conclusion, Cu2O-NPs are genotoxic to wheat seedlings, with consequences on the growth and development of wheat seedlings and root morphology.

Monitoring of changes in 5-n-alkylresorcinols during wheat seedling development.

For seven days of wheat growth, caryopsis remained the main source of 5-n-alkylresorcinols with C19 and/or C21 homolog as a main compound. Shoot contained small amount of these phenolic lipids; their average content was 3.23% of level obtained in the whole seedling. Moreover, 41.38% of resorcinolic lipids of seven-day-old shoot was accumulated in part of leaf covered by coleoptile. Interestingly, a removal of 1.07% of the primary pool of kernel alkylresorcinols by short-term washing (10s) of wheat seed with acetone before planting decreased their level only in seed of seven-day old seedling. Compared to the respective controls, this treatment did not affect the amount of these lipids in the green part of seedling that proved that de novo synthesis of 5-n-alkylresorcinols takes place in shoots. The very similar homolog profiles of these lipids in four- and seven-day-old shoots turned out to be markedly less diversified than those found in respective seed samples. Compared to the mature wheat caryopsis, the rise in the content of very-long-chain homologs was observed only in the oldest shoot. Their increased accumulation was probably connected with formation of cuticular layer providing the defensive barrier against various phytopathogens.

Desenvolvimento inicial de plantas de trigo em resposta à associação com bactérias diazotróficas e níveis de irrigação por solução ácida

The cultivated species have ideal soil pH for absorption of nutrients and proper development, it is necessary to know the performance of nitrogen-fixing bacteria and promoting plant growth (PGPBs) at different levels of acidity, in order to increase the results to elevate crop yields with the use of technology inoculation of seeds of gramineous. Thereby, the present study aimed to determine the initial development of wheat seedlings subjected to inoculation with Azospirillum brasilense and Herbaspirillum seropedicae at different levels of acidity irrigating solution in autoclaved sand substrate. Adopted the randomized block design (RBD) in a 3x4 factorial design with three levels of acidity of the solution (pH 4.5, 5.5 and 6.5) and the second factor [without inoculation, seeds inoculated at the time of seeding with A. brasilense (AbV5), H. seropedicae (SmR1) and association of the two strains AbV5+ SmR1. Increased root length was detected at pH 4.5 compared to pH 5.5 and 6.5 regardless or not with A. brasilense and H. seropedicae. Inoculation with A. brasilense promotes greater diameter collect, regardless of the pH and increase in root volume to pH 6.5 alone or associated with H. seropedicae.

Effects of high CO2 concentration, drought, and their interaction on different stay-green wheat seedlings

In this study, a pot experiment was carried out to examine the effects of high CO2 concentration, drought and interaction on seedling growth traits, biomass accumulation and physiological characteristics of different stay-green wheat seedlings, with a stay-green wheat variety Yannong 19 and a non stay-green wheat variety Hanxuan 3 as test materials. There were four treatments in the Open Top Chamber with factorial of CO2 concentration (370 μmol·mol-1 vs 550 μmol·mol-1) and drought (45%-55% vs. 75%-85% of field water-holding capacity). Drought significantly inhibited the growth and development of wheat seedlings, while CO2 concentration significantly increased the number of tillers and promoted the growth and development of wheat seedlings. Under drought condition, high CO2 concentration increased the number of tillers of Hanxuan 3 and Yannong 19 by 61.0% and 42.3%, respectively. Under both water conditions, high CO2 concentration significantly increased the biomass of wheat seedlings, and decreased the content of peroxidase and proline in leaves. Under drought condition, high CO2 concentration showed stronger "fertilizer effect". Furthermore, different varieties had different responses to high CO2 concentration, with higher sensitivity of Hanxuan 3 to enhancement of CO2 concentration. Under the scenario of increasing CO2 concentration, the amount of irrigation water applied to a field can be appropriately reduced for efficient use of water resources. Meanwhile, it is necessary to pay attention to the selection of suitable wheat varieties.

Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress

Aim. To study the effect of polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl) on morphophysiological and biochemical parameters of wheat seedlings in the presence of toxic concentration of copper ions. Methods. The growth-stimulating activity of PHMG-Cl in pure state, as well as in the presence of elevated concentrations of copper ions was studied by phytotesting method using wheat seed as a test culture. Physico-chemical interaction between PHMG-Cl and CuSO4 in water solutions was studied by spectrophotometry. Results. Wheat seed treatment with water solutions of polymeric biocide PHMG-Cl in the concentration range of 0.01–0.1 % causes an increase in the length of roots and shoots by over 50 % after 7 days of incubation. The concentration of PHMG-Cl of 1 % was found to have a depressive effect on growth characteristics of the tested plants. This fact indicates that the phytotoxicity of PHMG-Cl is at least two orders of magnitude lower compared with a common pesticide copper sulfate. Thus, wheat seed soaking in 200 μM (0.003 %) water solution of copper sulfate causes reduced length of shoots and roots (by 65 and 34 %, respectively), as well as reduced weight of shoots (by 20 %) and roots (by 74 %) The analysis of green mass showed signifi cantly reduced content of carotenoids (by 26 %) and water soluble fl avonoids (by 37 %), as well as chlorophyll a/b ratio (by 18 %) that indicates decreased plant metabolic activity. However, the toxic effect of copper sulfate ions signifi cantly diminished in the presence of PHMG-Cl (0.01 %). Thus, the length of shoots and roots was found to increase by 24 and 64 %, respectively, compared to the samples treated with CuSO4. Moreover, the content of pigments and fl avonoids in the green mass of seedlings reached the level of control samples. The results of spectrophotometric studies indicate the absence of physico-chemical interaction between PHMG-Cl and copper sulfate in water solutions. Conclusions. The polymeric biocide PHMG-Cl effectively stimulates growth and development of wheat seedlings in the concentration range of its water solutions of 0.01–0.1 %. In the presence of toxic copper sulfate concentration, PHMG-Cl does not possess the growth stimulating activity. However, the polymeric biocide signifi cantly improves copper stress resistance of plants, and normalizes their morpho-physiological indicators, photosynthetic characteristics and secondary exchange.

Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress

BackgroundHexaploid wheat is an important cereal crop that has been targeted to enhance grain micronutrient content including zinc (Zn) and iron (Fe). In this direction, modulating the expression of plant transporters involved in Fe and Zn homeostasis has proven to be one of the promising approaches. The present work was undertaken to identify wheat zinc-induced facilitator-like (ZIFL) family of transporters. The wheat ZIFL genes were characterized for their transcriptional expression response during micronutrient fluctuations and exposure to multiple heavy metals.ResultsThe genome-wide analyses resulted in identification of fifteen putative TaZIFL-like genes, which were distributed only on Chromosome 3, 4 and 5. Wheat ZIFL proteins subjected to the phylogenetic analysis showed the uniform distribution along with rice, Arabidopsis and maize. In-silico analysis of the promoters of the wheat ZIFL genes demonstrated the presence of multiple metal binding sites including those which are involved in Fe and heavy metal homeostasis. Quantitative real-time PCR analysis of wheat ZIFL genes suggested the differential regulation of the transcripts in both roots and shoots under Zn surplus and also during Fe deficiency. Specifically, in roots, TaZIFL2.3, TaZIFL4.1, TaZIFL4.2, TaZIFL5, TaZIFL6.1 and TaZIFL6.2 were significantly up-regulated by both Zn and Fe. This suggested that ZIFL could possibly be regulated by both the nutrient stress in a tissue specific manner. When exposed to heavy metals, TaZIFL4.2 and TaZIFL7.1 show significant up-regulation, whereas TaZIFL5 and TaZIFL6.2 remained almost unaffected.ConclusionThis is the first report for detailed analysis of wheat ZIFL genes. ZIFL genes also encode for transporter of mugineic acid (TOM) proteins, that are involved in the release of phytosiderophores to enhance Fe/Zn uptake. The detailed expression analysis suggests the varying expression patterns during development of wheat seedlings and also against abiotic/biotic stresses. Overall, this study will lay foundation to prioritize functional assessment of the candidate ZIFL as a putative TOM protein in wheat.

Влияние бактеризации Aeromonas media GS4 и Pseudomonas extremorientalis PhS1 на проростки пшеницы в различных абиотических условиях

We studied the effect of soft wheat seed treatment (Triticum aestivum L.) with two bacterial strains (Aeromonas media GS4 and Pseudomonas extremorientalis PhS1) isolated from earthworm coprolites on the growth and development of wheat seedlings in a 12-day laboratory experiment, as well as on root rot disease and the activity of guaiacoldependant peroxidase under optimal conditions and abiotic stress (elevated and low temperatures and moisture content). We established that growing nonbacterized wheat plants under stress abiotic conditions reduced the height of plants compared to growing under optimal abiotic conditions, and seed bacterization with P. extremorientalis PhS1 strain increased wheat plant height (by 9-15%) under stress abiotic conditions compared to the nonbacterized plants. Bacterization with both strains decreased infestation of wheat seedlings (2.5-4 times) by root rots under unfavorable abiotic conditions compared to nonbacterized plants. In addition, under optimal and arid conditions, bacterization with P. extremorientalis PhS1 strain was the most effective, and under humid conditions it was bacterization with A. media GS4 strain. We showed that the activity of guaiacoldependant peroxidase correlates with the development of plant resistance to abiotic stress. In our experiments, plant bacterization resulted in a 2-fold increase in peroxidase activity both in leaves and roots of wheat plants compared to the nonbacterized plants. As the result, the ability of bacteria to activate peroxidase can serve as an information indicator of strengthening protective mechanisms of plants during bacterization. The paper contains 4 Figures and 34 References.