Abstract

The cultivated species have ideal soil pH for absorption of nutrients and proper development, it is necessary to know the performance of nitrogen-fixing bacteria and promoting plant growth (PGPBs) at different levels of acidity, in order to increase the results to elevate crop yields with the use of technology inoculation of seeds of gramineous. Thereby, the present study aimed to determine the initial development of wheat seedlings subjected to inoculation with Azospirillum brasilense and Herbaspirillum seropedicae at different levels of acidity irrigating solution in autoclaved sand substrate. Adopted the randomized block design (RBD) in a 3x4 factorial design with three levels of acidity of the solution (pH 4.5, 5.5 and 6.5) and the second factor [without inoculation, seeds inoculated at the time of seeding with A. brasilense (AbV5), H. seropedicae (SmR1) and association of the two strains AbV5+ SmR1. Increased root length was detected at pH 4.5 compared to pH 5.5 and 6.5 regardless or not with A. brasilense and H. seropedicae. Inoculation with A. brasilense promotes greater diameter collect, regardless of the pH and increase in root volume to pH 6.5 alone or associated with H. seropedicae.

Highlights

  • Diazotrophic bacteria can be used to partially replace nitrogen fertilizers (Novakowiski et al, 2011; Piccinin et al, 2013), because they promote increase in root length, number of roots and radicial roots, being called plant growth promoting bacteria (PGPBs), due the production and estimulaton of hormone that potentiate the plant development (Egamberdieva, 2012)

  • The cultivated species have ideal soil pH for absorption of nutrients and proper development, it is necessary to know the performance of nitrogen-fixing bacteria and promoting plant growth (PGPBs) at different levels of acidity, in order to increase the results to elevate crop yields with the use of technology inoculation of seeds of gramineous

  • Thereby, the present study aimed to determine the initial development of wheat seedlings subjected to inoculation with Azospirillum brasilense and Herbaspirillum seropedicae at different levels of acidity irrigating solution in autoclaved sand substrate

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Summary

Introduction

Diazotrophic bacteria can be used to partially replace nitrogen fertilizers (Novakowiski et al, 2011; Piccinin et al, 2013), because they promote increase in root length, number of roots and radicial roots, being called plant growth promoting bacteria (PGPBs), due the production and estimulaton of hormone that potentiate the plant development (Egamberdieva, 2012). The activity of the PGPBs grow at an ideal pH range of 6.0 to 7.0 and few grow well at pH below 5.0 (Rodrigues et al, 2006; Ali et al, 2009) In this context, it is appropriate to know the relationship between plant and bacteria, because the cultivated species lack an ideal soil pH for adequate nutrient absorption and adequate development. The objective of this work was to verify the initial development of wheat seedlings submitted to inoculation of A. brasilense and H. seropedicae at different levels of acidity of the irrigating solution in autoclaved sand substrate. Variance analysis was performed and Tukey's test was applied at 5% error probability for the means provided for both different pH levels of the irrigating solution and seed inoculation

Results and discussion
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Journal of Biotchnology and
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