The high quality of the environment, protection of natural resources, efficient use of natural resources, and elimination of environmental burdens are the main priorities of sustainable development. Therefore, enterprises around the world have to manage the environment wisely, while avoiding negative effects on it. The aim of the paper was to examine the use of green and reverse logistics in the conditions of sustainable development in enterprises in Slovakia. The intention of the paper was to provide a theoretical overview of green and reverse logistics at home and abroad on the basis of the elaboration of a scientific bibliographic-information apparatus. Subsequent practical implementation of the questionnaire research and statistical verification of research results provides a basis for practical recommendations and conclusions for enterprises operating in Slovakia. The research topic of the paper was green and reverse logistics in companies in Slovakia. The research problem was the implementation and use of modern logistics under the influence of the environment. Based on these facts, statistical hypotheses were determined and subsequently tested. Descriptive statistics and inference statistics were used to interpret the research results. Based on the research, we found that the dominant position in green and reverse logistics in Slovakia was achieved by large production enterprises from the automotive industry, which operate in western Slovakia. The analysed enterprises in Slovakia use voluntary tools of environmental policy and the most important environmental tool is corporate social responsibility. Elements of environmental policy are used primarily in the logistics process of warehousing and storage. The biggest barrier in the implementation of elements of environmental logistics analysed by enterprises is the lack of financial resources. The implementation of green logistics and reverse logistics enables the analysed enterprises in Slovakia to improve customer-supplier relations. We can say that despite the government’s efforts, measures to minimize the environmental impact and growing public awareness of environmental issues, environmental problems persist. Initiatives can be seen at the global and national levels of enterprises that make quick decisions and set up new processes in environmental management.