Western Siberia Region Research Articles

Parameters of Different Cloud Types over the Natural Zones of Western Siberia According to MODIS Satellite Data

The technique of studying seasonal variations in cloud parameters over regions of Western Siberia according to satellite data is presented. Five natural zones have been identified: tundra, forest tundra, swamps, taiga, and forest steppe. A combined “summer” and “winter” classification of cloudiness is introduced; the classification includes 16 and 12 cloud types, respectively. Cloudiness images are classified using an algorithm based on neural network technologies and fuzzy logic methods. Results of analysis of seasonal variations in some parameters of different types of cloudiness and their repeatability over the considered regions of western Siberia based on MODIS satellite data for 2017 are discussed. The dependences of the seasonal variability of cloudiness parameters agree well with known literature data on ground-based observations, which corroborates the effectiveness of the proposed technique.

Hydrogeochemistry of authigenic mineral formation in Upper Jurassic sediments (the Nadym-Taz interfluve area, Arctic regions of Western Siberia)

This paper presents a novel numerical modelling approach to physicochemical evolution of water – rock interactions in Upper Jurassic sediments in the Nadym–Taz interfluve (Arctic regions of Western Siberia). The results show that the authigenic mineral formation proceeds continuously and strictly successively (kaolinite – montmorillonite – illite – mica – chlorite – albite – microcline), involving definite geochemical parameters of the medium (pH, concentrations of SiO2, Al, Na, K, Ca, Mg in solution). For example, the widely spread kaolinite cement forms in the medium with near neutral pH (on average, рH = 7.2) with silica concentration in solution 15–20 mg/dm3, while the formation of authigenic illite begins at silica concentration of 25–40 mg/dm3 and pH about 7.3–7.4. Despite rather low salinity (up to 63 g/dm3) of the investigated groundwater and long-term interaction of water with the rocks (spanning ca. 165 Ma), the equilibrium with primary (endogenous) minerals such as albite, anorthite, microcline is practically unobserved. At the same time, the groundwater is in equilibrium with mica, illite, Ca–, Na– and Mg–montmorillonites, kaolinite, more rarely with Mg–chlorite. The state of groundwater equilibrium with primary aluminosilicate minerals is always challenged by a carbonate barrier, so rocks carbonatization in various degree is observed almost everywhere.

Opisthorchis felineusinfection, risks, and morbidity in rural Western Siberia, Russian Federation.

BackgroundThe liver fluke, Opisthorchis felineus, is widely distributed throughout Europe and large parts of the Russian Federation. In Western Siberia, information about opisthorchiasis is lacking although infection may lead to severe liver and bile duct diseases. We aimed to assess the current prevalence of O. felineus infection along with associated risk factors and morbidity in rural Western Siberia.MethodsWe conducted a community-based, cross-sectional study in the rural Shegarskiy district, Tomsk Oblast, Russian Federation. All household members (≥ 7 years) present on the survey day were enrolled (n = 600). Two stool samples per person were examined for helminth eggs, using PARASEP (DiaSys Ltd, UK). The number of eggs per gram (EPG) of feces was recorded. Each study participant was interviewed to determine risk factors, using a pre-tested questionnaire. An abdominal ultrasonography examination of liver and bile ducts was performed with a mobile, high resolution ultrasound device. In total, 488 persons completed assessments (two stool samples, completed questionnaires); of those, 436 individuals had an ultrasonography (US) examination.ResultsWe observed a prevalence of O. felineus infection of 60.2%. Significant risk factors for infection were the consumption of river fish (odds ratio from adjusted analysis [aOR] 2.4, 95% CI 1.52–3.95, p<0.001), particularly stock fish (OR from multivariable analysis [mOR] 3.2, 95% CI 2.63–3.80, p<0.001), smoked fish (mOR 1.5, 95% CI 1.24–1.72, p<0.001), frozen fish (mOR 1.6, 95% CI 1.29–2.02, p<0.001), and raw fish (mOR 1.4, 95% CI 1.05–1.84, p = 0.02); and fishing activities (mOR 1.2, 95% CI 1.03–1.43, p = 0.019). Women had a higher risk of infection than men. Infection was associated positively with age and negatively with socio-economic status. The respondents’ general awareness of opisthorchiasis was quite high (93.2%), but their knowledge about infection transmission and prevention was insufficient. Children aged 7–18 years old had a lower level of awareness compared to adults. The abdominal ultrasonography results demonstrated a strong association between O. felineus infection and gallbladder stones (mOR 2.8, 95% CI 1.33–6.04, p = 0.007) and periductal fibrosis of intrahepatic bile ducts (mOR 1.9, 95% CI 1.08–3.46, p = 0.026).ConclusionO. felineus infection is highly prevalent in rural regions of Western Siberia, and associated with severe hepatobiliary pathology. Identified risk factors will be used to develop a comprehensive targeted O. felineus infection control program.

MOBILE FORMS OF METALS IN SOILS IN THE NADYM-PUR INTERFLUVE (WESTERN SIBERIA)

The total concentrations of metals (Fe, Mn, Cu, Ni, Cr, Sr) and their mobile forms extracted by an ammonium acetate buffer (pH = 4,8) were studied in soils samples collected from Nadym-Pur interfluve (West Siberia), near the northern limit of the taiga zone. The heavy metal content was determined in 55 background soil samples and in 25 pyrogenic ones. Gross content was determined on a spectrometer "SPECTROSCAN MAX-GV" using x-ray fluorescence analysis. Mobile forms of metals were determined on a ContrAA 700 atomic absorption spectrometer by flame and electrothermal methods. The statistics are calculated using the Excel software package. The study confirmed the weak accumulation of metals in the soils of the northern regions of Western Siberia. The total content below the soil world average for Fe is 5 times, Mn - 3 times, Sr - 2 times, Cr - 1,4 times. The content of mobile forms varies widely depending on the amount of organic matter. The percentage of mobile forms of metals from the total content was: Cr - 0.84%, Fe - 1,2%, Sr - 3,8%, Mn - 8,7%, Ni - 15%. In the profile of podzols, the distribution of mobile forms of metals has an eluvial-illuvial character. Mn and Sr accumulate intensively in the surface organogenic horizons, and the Fe content increases in the BF horizon. Minimum values are marked in horizon E. In pyrogenic soils, there is an increase in the content of mobile forms of metals due to the intake of ash. Metal content usually not exceeded the maximal permissible concentrations for soils.

Water Chemistry of Arctic Lakes under Airborne Contamination of Watersheds

The data on the metal contents and acidification of small lakes caused by airborne contamination of the watershed in three industrial regions of the Arctic—European Russia (Kola region), Western (Yamal-Nenets region) and Eastern Siberia (Norilsk region)—have been presented for the first time. It has been proven that acidification and enrichment by metals of water connect with sulfur dioxide and metals emissions from copper–nickel smelters, contaminating the catchments, with associated gas burning during raw hydrocarbon production. To assess the effects of acid deposition, critical loads and their exceeds were calculated: exceeded by 56% and 12.5%, respectively, in lakes in the Kola region and in the north of Western Siberia; the catchments of the East Siberian region are resistant to acidification. Water enrichment factors (EF) by elements were calculated to show that the waters of the Norilsk and Kola regions are enriched with Ni, Cd, As, Sb and Se as a result of emissions from copper–nickel smelters. The oil and gas industry in the northern regions of Western Siberia lead to the increase in V, Pb and Mo concentrations in the waters. The high values of EF and excess of acidity critical loads for water are explained by the local and transboundary pollution impacts on the catchment of small lakes.

Micropaleontological and Lithogeochemical Characteristics of the Turtas Formation (Upper Oligocene), Western Siberia

This work presents the results of integrated micropaleontological and lithogeochemical analysis of Upper Oligocene continental deposits of the Turtas Formation from the Zyryanskaya-1 borehole (southwestern part of the Tyumen Region, Western Siberia). The predominance of freshwater planktonic diatoms at the subordinate significance of benthic species, as well as the presence of gemmuloscleres of sponges, indicates the coastal sedimentation conditions in a freshwater basin varying in depth from 3 to 50 m. The studied palynocomplex is similar to the spore-and-pollen spectra of the Turtas Formation of the Lower Ob Region, but differs from palynocomplexes of the same formation spread over the southern regions of Western Siberia in a low amount of Taxodiaceae/Cupressaceae and Betulaceae pollen, as well as the absence of Fagaceae pollen and Pseudokomewuia freshwater dinocysts. The revealed differences can be explained both by facies features and by variations in the volume of the Turtas Formation in the currently known localities. The data on the microelemental composition of samples, as well as geochemical and petrogeochemical modules, indicate that the accumulation of the Turtas Formation occurred in the coastal areas of a low-mineralized basin under relatively warm humid climatic conditions.

Geochemistry of Water-Dissolved Gases of Oil-and-Gas Bearing Deposits in Northern and Arctic Regions of Western Siberia

The paper presents a pioneering attempt in the last 35 years, to summarize the accumulated data on geochemistry of water-dissolved gases of oil and gas-bearing deposits in northern and arctic regions of Western Siberia. Water- dissolved gases from all the studied oil/gas fields are characterized by absolute predominance (up to 75-99 vol.%) of the methane series hydrocarbons in their composition. The identified (after the L.M. Zorkin’s classification) four classes of hydrocarbon type of water-dissolved gases (vol.%) are designated as: dry (ΣHH < 1), lean (ΣHH = 1-3), semi-fat (ΣHH = 3-5) and fat (ΣHH> 5). The total gas saturation of groundwaters ranges from 0.3 to 5.7 L/L with CH4 concentrations averaging 95.5 vol.% in the Aptian-Albian-Cenomanian to 83.3 vol.% in the Lower-Middle Jurassic complexes. The levels of homologues ΣHH (C2H6, C3H8, C4H10, C5H12 and C6H14) tend to increase with depth: from 1.34 vol.% in the Aptian-Albian-Cenomanian to 11.67 vol.% in the Lower-Middle Jurassic complex. The maximum ΣHH concentrations reaching 30 vol.% were found at the Neocomian base in the peripheral waters of oil accumulations. CO2 levels show an increasing trend with depth, whereas N2 concentrations tend to decrease. Given the increased depths and more severe temperature conditions, the level of hydrocarbon generation remains high, however hydrocarbon series is characterized by a shift towards liquid components and methane homologues (the main zone of oil/fat gases generation). Therefore, the revealed in the Lower-Middle Jurassic complex highly gas-saturated groundwaters (up to 4.5-5.7 L/L) enriched with methane homologues allow to assess their hydrocarbon prospects as very high.

Economic Factors of Mortality from Infectious Diseases in Russian Regions

infectious disease mortality occupies a small share in the structure of mortality in developed countries. However, it is concentrated in relatively young ages, and it entails significant economic costs. From 1990 to 2018, Russian regions showed different dynamics of mortality from infectious diseases. Some regions managed to reverse the negative growth trend in mortality in the mid-2000s, but there are regions where growth in mortality continues to this day. Mortality from infectious diseases in the regions safest and least well on the indicator differs by a factor of 10. The worst dynamics are demonstrated by Volga, Urals, and Western Siberia regions as well as Altai Krai, Irkutsk Region, Primorsky Krai, and Chukotka. The purpose of this article is to study the economic factors determining mortality from infectious diseases in Russian regions. Estimating a fixed effect panel model based on data by the Federal State Statistics Service shows that regions with higher per capita cash incomes demonstrate lower mortality from infectious diseases. Alcohol consumption is a factor associated with higher mortality from infectious diseases. Mortality from infectious diseases is higher in regions with a larger population per doctor and with a lower capacity of outpatient facilities. important information for decision-makers responsible for reforming the healthcare system in terms of reducing the number of doctors consists in the presence of high elasticity of mortality from infectious diseases to the change in the population per doctor even in the absence of epidemics. Conclusions of the study could serve as a guideline for developing parameters of state health programs.

Hydrogeochemistry of the authigenic mineral-forming processes (a case study of Oxfordian deposits in the Ob-Irtysh interfluve)

Results of the pioneering numerical physicochemical modeling of interactions in the water – rock system on the example of Oxfordian deposits in southern regions of the Ob-Irtysh interfluve (southeastern regions of Western Siberia) are presented.It has been established that, despite the groundwaters having low salinity (up to 46 g/dm3) of and their persistent interactions with rocks (during tens, or probably hundreds of millions of years), they practically have no equilibrium with primary (endogenous) minerals: albite, anortite, microcline.At the same time, groundwater is in equilibrium with micaceous minerals, Ca-, Na - and Mg-montmorillonites, kaolinite, Mg-chlorite. There is always a carbonate barrier in the way of groundwater attaining an equilibrium with primary aluminosilicate minerals. As a result of the detailed petrographic studies, the genetic series of authigenic minerals of the diagenetic and catagenetic phases were established in the studied silty-sandy rocks. Relict diagenetic minerals are represented by glauconite, pyrite, siderite, calcite of the first generation of precipitation, kaolinite, anatase and leucoxene. Catagenetic minerals are considerably dominated by calcite of the second and third generations of precipitation, quartz, kaolinite, whereas siderite, chlorite, albite, dolomite are developed in minor amounts. The results of petrographic studies have corroborated the thermodynamic calculations.

Problems of the organization and activities of bodies of the Soviet justice in the national regions of the north of the Tyumen Region Tobol District in 1920-1927

This article reveals the history of the formation of the early Soviet justice authorities in the northern regions of Western Siberia and the Urals in the 1920s using archival materials. The activities of the first judges, investigators, bailiffs, and prosecutors show the state policy in introducing the principles of Soviet law in an isolated region of Russia. This paper explains the role of the Soviet justice authorities in strengthening the new government in the isolated regions of the RSFSR. The information provided expands our knowledge about the process of establishing the law enforcement system in Russia. The purpose of the article is to identify the specifics of the activities of the Soviet justice authorities in the conditions of the North of Russia in the 1920s. Native justice authorities are not the subject of research in this article. The sources of the research include the documents of the bolshevik, Soviet, and law enforcement agencies stored in the archives of Tyumen, Tobolsk, Yekaterinburg, Omsk, Khanty-Mansiysk, and Surgut. This article has used the system-structural and the comparative analysis methods. The territorial scope of the study includes two northern districts of the Tyumen Region and five northern areas of the Tobolsk District of the Ural region. The chronological framework is defined by the period in 1920-1927. The first date is the moment of the creation of the first Soviet judicial bodies in the Tyumen province. The second date is related to the beginning of the Cultural Revolution and the transfer of managerial powers to the district courts. This moment starts the rejection of the model of Soviet justice created in 1922. This study has revealed the inability of the Soviet state to establish a permanent system of repressive organs in the north of the Urals. The management structure, territorial structure, and staff changed with kaleidoscopic speed. Young communists, mobilized to serve in courts and as prosecutors, tried to pursue the Bolshevik policy. Yet the outrageous illiteracy and severe climatic conditions forced them to leave the North, and the party bodies could not stop this process.

A Historical and Legal Analysis of Certain Types of Crimes Among the Native People of the North in the Second Half of the 1920’s (on the Example of the Modern Territory of the KMAO–Yugra)

The crime among the native people of the North and the activities of national courts in the northern regions of Western Siberia are attracting increasing attention from researchers. This article is based on unpublished archival materials. The purpose of the article is to highlight the characteristic features of crime among the native population in the second half of the 1920 ’ s on the example of the modern territory of the KMAO–Yugra. The author comes to the conclusion that in the mentioned period and territory two main groups of crimes dominated: against the government and family.

МОДЕЛИРОВАНИЕ УГЛЕРОДНО-АЗОТНОГО ЦИКЛА ТУНДРЫ В ГОЛОЦЕНЕ

The observed increase in near-surface temperature can cause degradation of permafrost and an increase in greenhouse gas emissions. At the same time, the productivity of the tundra increases, and more intense photosynthesis means an increase in the absorption of carbon from the atmosphere. Therefore, the development of models of the carbonnitrogen cycle to study the contribution of tundra ecosystems is a special challenge. In this work, the modeling of carbon and nitrogen dynamics in tundra ecosystems of northern regions of Western Siberia were carried out using the reservoirflow model including vegetation, organic carbon compounds, dissolved organic and inorganic nitrogen. Input data of the atmospheric forcing were set according to the results of calculations with CLIMBER-2 model for the last 10 thousand years. Model estimates show a regional increase in mean annual air temperature and a decrease in the annual range over a period of 10–5.5 thousand years ago due to an increase in winter temperatures in this region. According to the results of calculations, carbon stocks in soil increase monotonically during the period under consideration. Calculated soil C values for modern climatic conditions are 14700 gC/m2 . The reserves of nitrate and ammonium forms of nitrogen are stabilized at the beginning of the calculation period. The results of calculations show that tundra ecosystems within the last 10 thousand years, including those under conditions of near-surface warming with increasing winter temperature and minor changes in summer temperature could accumulate organic matter, absorbing carbon-containing greenhouse gases from the atmosphere.

Natural cracking and the methods of its research when searching for oil and gas deposits (for example the southern areas of the Tyumen region)

Thanks to the complex approach to the study of natural cracking (use of modern remote and geochemical research) in the southern regions of Western Siberia (south of theTyumen region), large regional tectonic blocks and depression zones have been identified by space and geological-geophysical data. Their potential for detecting hydrocarbon deposits was evaluated, taking into account the geodynamic and fluid dynamic approaches.

Religious Authority and Ordinary Ethics in Muslim Migration Contexts

The article concerns religious authority among Muslim migrants in Russia. The fieldwork was carried out in 2018 in Tomsk, a city in southwestern Siberia. The focus of this study is the religious and wider social practices of those Muslim migrants who are considered leaders of local microcommunities, enjoy respect within their religious community, and have steadfast religious authority within their circles. These practices are considered in their local religious and migrant contexts through the prism of such concepts as religious individualism, everyday lived Islam, and tactical religion. In this article I am interested in the dynamics of religious identity among Muslim migrants in Siberia who are considered to be local leaders, as well as their religious introspection. I show how Muslim norms and regulations are recognized in the framework of everyday communication and socialization on a horizontal level. I emphasize in my research the importance of the everyday in the formation of individual religiosity and consider how a local Muslim environment is being built around individual key figures outside the mosques. The article answers the following questions: what role certain Muslims, known only in a narrow circle, play in constructing the religiosity of their countrymen; how various forms of authority (financial, migrational, professional) correlate with and are supported by religious authority; and how individual Muslims are changing the religious space of Russian cities. Based on my previous fieldwork, I argue that the main ideas of the article and the observations reflected in it are also relevant for my other field sites, including another southwestern Siberian city of Irkutsk, the northern regions of Western Siberia (Yamal-Nenets and Khanty-Mansi Autonomous Okrugs), and even Moscow. Article in Russian DOI: 10.25285/2078-1938-2020-12-1-81-105

Microbiota and Paleoenvironments of the Turtas Paleobasin, Late Oligocene, Western Siberia

The Turtas Formation (the Upper Oligocene of Western Siberia), uncovered by the Zyryanka-1 Borehole, contain diverse assemblage of microfossils. The ecological composition (freshwater diatoms, spicules and gemmuloscleres of freshwater sponges, spores and pollen, as well as zygospores of Zygnematales algae) indicates the nonmarine nature of the Early Turtas Basin. In contrast to the assemblage of the Zhuravka horizon (southern regions of Western Siberia), the absence of Fagaceae and dinocysts of the genus Pseudokomewuia, as well as low numbers of Taxodiaceae–Cupressaceae and Betulaceae, are probably explained by local characteristics.

The specialization areas of grain production focused on the export of products

The country’s export objectives, aimed at the growth of the Russian economy, are best addressed by highlighting the competitive advantages of its border areas. In wheat exports, as of today, Russia ranks first in the world, but its gross harvest rises in conditions of low-quality grain production. The paper analyzes the production parameters of spring wheat cultivation, explores the dynamics of its sown areas, gross yield, and yield by federal districts for the period 2001-2017. The article calculated the structure of crops and wheat production, indicators of its effectiveness depending on the quality of grain in the agricultural organizations of the country. The conditions for economic growth of individual border areas of the country are determined on the basis of the development of efficient production of strong and hard varieties of spring wheat in the regions of Western Siberia. A mechanism has been developed and proposed for creating a single large-scale West Siberian specialized zone for the sustainable production of high-quality spring wheat commercial grain with sales to the neighboring countries of the Asian continent.

Stability of relic methane hydrates under climatic changes in the Holocene

The results of numerical modeling of the thermal regime of high-latitude permafrost regions of Western Siberia (the Yamal Peninsula) for the last 6 thousand years are presented. Thermobaric conditions for stability and dissociation of continental methane hydrates are defined. It is shown that at the present time relict methane hydrates can exist at depths of up to 150 m in the strata of frozen soil of Yamal above the modern boundary of the stability zone, having “survived” warming in the Mid-Holocene warm period (about 6,000-5,000 years ago) at negative temperatures in the permafrost. According to the results obtained, the current warming in Yamal region exceeds the warming of the Mid-Holocene. The increase of the temperature of the permafrost layer can reduce the strength of the soil and lead to dissociation of the near-surface gas hydrates.

Physical-chemical and water-physical properties of agricultural soils on inter-mountain hollows of Mountain Altai

The purpose of the research is the study the basic physical,chemical and water-physical properties of soils of high and medium mountain basins of the Altai mountains under different types of agricultural use (arable land, hayfields, pasture). The main features of chestnut and light chestnut soils of high-mountain basins, dark chestnut soils and common chernozem of mid-mountain basins are established. These are a light granulometric composition with a high content of coarse soil, a sharp decrease of humus content and cation exchange capacity down the profile, slightly alkaline reaction of medium, and perfect structural state, except for the lower horizons of light chestnut soil. By density of the humus horizon, the soils of hayland and pasture belong to the soils rich in organic matter. Considerable density is noted also in the chernozem ordinary under an arable land where for many decades usual dump plowing is applied, minimum - in the dark-chestnut soil under an arable land where in recent years processing is carried out by a diskator which basic purpose - preparation of the soil for crops without preliminary plowing. The density of arable horizon on arable land correspond to typical values for a cultivated or a new-ploughed arable land. With the depth of the soil profile, this ratio increases, but it is lower than typical values for the subsurface horizons. The density of the solid phase varies slightly. In the upper organogenic horizons of the hayland and pasture, it is low due to the occurrence of organic matter. In the lower horizons, it is slightly higher and corresponds to the density of the solid phase for mineral soils. The porosity of the studied soils is in inverse proportion to density. The porosity of the upper horizons is perfect, and the horizons correspond to the cultural-arable layer. The porosity of the lower horizons is satisfactory. The hygroscopic moisture content of the soils under study varies in the wide range (0,7-9,9%). The maximum hygroscopicity in the sandy loam horizons is low (2-4%) and it is higher in loamy horizons (4-7%). The permanent wilting point ranges from 2,7 to 11,7%, and total water capacity - from 25,9 to 50,4%. Comparison of physical-chemical and water-physical properties of the studied soils of intermountain basins of the Altai Mountains with those of the same type of soils of the Altai territory and adjacent regions of Western Siberia indicates their proximity and even some advantage.

ПРОГНОЗ НЕФТЕГАЗОНОСНОСТИ ЮРСКИХ И ПАЛЕОЗОЙСКИХ ОТЛОЖЕНИЙ ЮЖНЫХ РАЙОНОВ ЗАПАДНО-СИБИРСКОГО БАССЕЙНА

Статья посвящена перспективам нефтегазоносности палеозойских и мезозойских комплексов в южных районах Западной Сибири. Для региона характерны сложнопостроенные неантиклинальные ловушки, связанные с полифациальным генезисом вмещающих толщ, особенностями тектонического и литологического строения. В связи с этим выявление перспективных земель для поисков залежей углеводородов невозможно без применения комплексного подхода, включающего геологические, литологические, тектонические и гидрогеологические исследования. Использование гидрогеологических критериев нефтегазоносности основано на ведущей роли подземных вод в процессах генерации, миграции и аккумуляции углеводородов. В процессе исследования установлены гидрогеохимические особенности подземных вод, выделены гидрогеохимические аномалии, тесно связанные с залежами углеводородов (I, B, Br, NH4 и др.). Охарактеризованы генетические типы подземных вод и степень их катагенетической преобразованности. Приведены основные результаты палеогидрогеохимических и палеогидродинамических реконструкций, выделены зоны нефтегазообразования и нефтегазонакопления, показаны возможные пути латеральной и вертикальной миграции флюидов. Изучены особенности геологического строения, выделены зоны развития улучшенных коллекторов. Совместное использование гидрогеологических и геологических показателей продуктивности недр позволило обосновать набор критериев нефтегазоносности, несколько различных для палеозойского, среднеюрского и верхнеюрского комплексов. С помощью предложенного комплекса критериев был осуществлен прогноз нефтегазоносности и оконтурены участки различных категорий перспективности (высокоперспективные, перспективные и земли с неясными перспективами, требующие дальнейшего изучения). Применение данного подхода будет способствовать локализации геологоразведочных работ и повысит эффективность геофизических методов при поиске новых залежей углеводородов в южных районах Западно-Сибирского бассейна.

Detection of bovine herpesvirus 4 DNA in cattle by realtime PCR.

BoHV-4 is poorly understood. Data on the circulation of the virus among animals and its role in infectious diseases insufficient. Aimes and goals. Development of real-time PCR for detecting the BoHV-4 and studying the frequency of its presence in samples from sick animals. The nucleotide sequences of the glycoprotein L gene served as a target for amplification. The sequences of reference strains published in GenBank were used to analyze and design the primers. Studies were conducted in 3 regions of Western Siberia on 5 large dairy farms. 27.7% of samples contained the virus. The virus was present as a monoagent in nasal cavity of calves (80.0%), lungs (46.2%) and bronchial lymph nodes (38.5%) in pneumonia. In the cases of diarrhea the virus was detected in 20%, and in cows with gynecological pathology in 10.0%. In respiratory diseases of calves the virus was detected in association with BoHV-1 (21.6%) and BoCV (20.3%), and in gynecological pathology of cows with BVDV1 (6%). According to the phylogenetic analysis of 5 identified virus isolates, four belonged to the American branch and one to the European branch. The circulation of American strains occurred in the territory of the Republic of Kazakhstan (1), Tyumen (1) and Novosibirsk (2) regions, and the European - in the Novosibirsk region. The search for viruses involved to the infectious pathology, as well as studying the genetic diversity of viruses circulating on a particular farm including imported from other countries, is relevant.