Duplex Stainless Steel (2304) steel plates of 6 mm thickness have been fabricated by one-side butt welding procedure employing Tungsten Inert Gas welding process at three different positions (i.e. Flat, Horizontal and Vertical). The effort of welding position on Macro-section and Microstructure of TIG welded specimen was observed. The welding current, filler material and speed was optimizing by carefully evaluating weld geometry and microstructure of different welding positions. Design of experiment was carried out using Taguchi technique to optimize the process parameters of welding. The input variable process parameters are welding current, speed, filler material and welding position, whereas output parameters are weld width, reinforcement, penetration and wetting angle. The experimental results illustrate that the joint of horizontal weld has more reinforcement and microstructure and macro-section includes three zones: weld zone, heat affected zone, thermal affected zone showed that unsymmetrical weld interface and thermo mechanically affected zone boundary was found in horizontal and vertical position joints. The formation of fine grains with less fusion area and less reinforcement were found in flat welding positions. Form all the samples it was found that the heat affected zone area, fusion angle and wetting angle is more for vertical position welding specimens. By analyzing macro-section and microstructure of joints it was observed the fusion area at root gap for vertical weld is three times more compared to flat welding position and root penetration is more at high welding current. It was found that the migrated grain boundaries are formed on the weld zones for all the weldments.