Weight Of Fruiting Bodies Research Articles

Comparison of Ultrasonic And Acoustic Sound Treatments on Grey Oyster Mushroom (Pleurotus Sajor-caju) Cultivated on Sawdust And Kenaf Waste

Sound stimulation and substrate are among the main factors that enhance the formation of fruiting bodies in order to increase the productivity of grey oyster mushroom. The main purpose of this study is to investigate the effects of ultrasonic and acoustics sound treatments on grey oyster mushroom (Pleurotus Sajor-caju) cultivated on rubber tree sawdust and kenaf waste. The growth performance, yield and post-harvest quality of grey oyster mushroom were determined. Mushroom bags were treated with ultrasonic and acoustic sound every 5 days intervals during mycelia growth. Sound treated mushroom bags had faster growth rate and treatment with acoustic sound appeared to be a better treatment among all the treatments. In terms of yield, sawdust obtained a relatively higher amount of total weight of fruiting bodies, greater biological efficiency and bigger number of fruiting bodies than kenaf waste. Acoustic sound contributed to a higher in yield but has the highest substrate utilization among all treatments. Sawdust shows no significant different (p>0.05) in physical characteristics such as pileus diameter compared to kenaf waste but both of them showed significant different (p<0.05) among different treatments. Acoustic sound had firmer and showed relatively darker compared to other treatments. Substrate of sawdust had a relatively higher of moisture content of pileus and stem than kenaf waste. Among all the treatments used, mushroom bag treated with acoustic and cultivated on sawdust was the best treatment due to is relatively faster growth performance and higher yield.

Edaphic and temporal patterns of Tuber melanosporum fruitbody traits and effect of localised peat-based amendment

In Tuber melanosporum cultivation, fruitbody traits are gaining relevance due to their increasing prominence on prices. We investigated the edaphic and temporal patterns of fruitbody traits and characterised the effect of truffle nests (localised peat-based amendment supplemented with T. melanosporum spores) on traits. We monitored fruitbody traits throughout two fruiting seasons in three blocks along a soil gradient. Each trait followed specific edaphic and temporal patterns. The number of fruitbodies per dig and spore maturity followed characteristic within-season trends, whereas fruitbody weight and infestation by truffle beetles were subject to complex interactions among edaphic and temporal variables, suggesting a relevant influence of annual environmental conditions. The application of truffle nests increased fruitbody depth, improved its shape and decreased infestation by truffle beetles. Nests increased the number of fruitbodies per dig, but only in two of the soils, suggesting a relevant role of the bulk soil/substrate interface in fruiting initiation. These results outline a complex scenario, with each trait being differently affected by environmental factors. In this scenario, nests proved to effectively modify several traits, although not always in the desired direction.

Evaluation of Organic Substrates for Outdoor Cultivation of Pleurotus Tuber-Regium

Pleurotus tuber-regium is a unique mushroom with a lot of benefits. Yield of the mushroom was evaluated when cultivated outdoor on agricultural beds different substrates (treatments) in order to assess the effect of the substrates. Four substrates used were topsoil, a mixture of topsoil and sawdust (2kg), a mixture of topsoil and poultry droppings (2kg), and a mixture of topsoil, poultry droppings (2kg) and sawdust (2kg). The design was Randomized Complete Block Design (RCBD) with four replicates. Results showed that mixture of topsoil and sawdust had the highest mean dry weight 3.54±0.29 for first flush while the least was 2.57±0.45gm for topsoil. For the second flush, the highest mean dry weight of 1.56±0.37 was for mixture of topsoil and sawdust while the least, 0.58±0.31gm was for topsoil, poultry droppings and sawdust. Results for dry weight of fruiting bodies, indicated that only the mixture of Topsoil & Poultry droppings and Topsoil, Poultry droppings & Sawdust had no significant difference (P>0.05), for first flush; there was significant difference between other treatments. Results for second flush had no significant difference (P>0.05) between Topsoil and Topsoil & Sawdust treatments; there was significant difference (P

Developmental, morphological and molecular variation of commercial Ganoderma spp. accessions from southern Vietnam

Abstract. Ho VT, Vo TNH, Le NG. 2019. Developmental, morphological and molecular variation of commercial Ganoderma spp. accessions from southern Vietnam. Biodiversitas 20: 3684-3689. In Vietnam, the market value of Ganoderma spp. increases markedly recently due to the high demand as an effective medicine. However, the breeding programs for this mushroom are still less effective due to the poor knowledge of genetic composition. This study was conducted to investigate the diversity of some commercial Ganoderma spp. accessions from southern Vietnam using both traditional techniques using developmental and morphological characterization, and molecular method using ISSR markers. A total of nine Ganoderma spp. samples were collected from production areas and research institutes in southern provinces of Vietnam and targeted for developmental and molecular characterization. Developmental and morphological variations among accessions were evaluation. In addition, 20 ISSR markers were also utilized for further understanding genetic composition of these mushrooms. The obtained measurement data show a large variation among the studied Ganoderma spp. accessions in term of mycelia growth, diameter, shape, and dried weight of fruiting bodies. Molecular data from 20 ISSR markers also supported genetic variation of the examined samples. Based on the results of this study, the samples of Ganoderma spp. accessions from southern Vietnam can be divided into two main groups which are not correlated to geographical locations of sample collection sites. The results are potential for classification and provide new insights for molecular assisted selection of Ganoderma spp.

Effects of Crude Oil on the Growth of Oyster Mushroom; &lt;i&gt;Pleurotus ostreatus&lt;/i&gt; (Jacaum ex.fr. Kummer)

: The effect of crude oil on polluted sawdust on the growth of Pleurotus ostreatus was evaluated. The experiment was laid in a completely randomized design (CRD) with five replicates. Data collected were subjected to analysis of variance (ANVOA) and the mean separation was done using Duncan Multiple Range test (DMRT) at the probability of 5%. Result showed that micro-morphological characteristics of P. ostreatus grown on sawdust polluted with crude oil at varying concentrations of 50 ml, 100ml and 150ml and allowed to be contaminated for 8, 10 and 12 day respectively before sowing the mycelia in the polluted sawdust. Marco-Morphological at 10ml level of crude oil polluted sawdust significantly reduced the marco-morphological features of the P. ostreatus mushroom harvest at different days compared to 50ml crude oil polluted sawdust, in 8 days, the cap length measurement are (4.43cm±1.10 – 6.55cm±1.10), for stipe length (3.31cm ± 0.01 - 7.41cm ± 0.02) while the fresh fruit body weight (115.2g ± 2.01 - 175.3g ± 2.01). It is observed that the growing of P. ostreatus using wastes will help to reduce the huge agricultural wastes within our environment that sometimes when not properly managed caused serious environmental pollution. The research therefore recommended for the use of sawdust in the growing of Pleurotus ostreatus mushroom species, since sawdust and mushroom are good remediators of crude oil pollution. The summary of the result indicated that increase in crude oil pollution significantly (P≤0.5) affected the macro morphological features of the oyster mushroom production.Keywords: Oyster mushroom, Pleurotus ostreatus, morphological parameters and crude oil.

Utilization of coconut water as inducing substance in the cultivation of Pleurotus ostreatus

Cultivation of edible mushrooms has been evaluated as an effective way for increasing income of people in developing countries. Pleurotus ostreatus, well known as an oyster mushroom is the most popular edible mushroom in Indonesia. They usually is cultivated using a sawdust as a main substrate. The aim of this research is to reveal the addition of coconut water, the byproduct of traditionally coconut process as an inducing substance in P. ostreatus cultivation. The results shown that the addition 25% (v/v) of coconut water in substrate of P. ostreatus induce the growth of mycelium. On the other hand, the addition of 50% (v/v) of coconut water affect in the day of fruit body formation become faster as well as increase the number of pileus (stalk) and the weight of fruit body.

Effect of Some Substrates on Production of Oyster Mushroom

This research was carried out at the Mushroom Research Laboratory, EL_Kassain Horticulture Research Station, Ismailia Governorate. The research was carried out for two successive seasons, 2016 and 2017. The object of this experiment was to study the effect of some of substrates (rice straw, wheat straw, bean straw, alfalfa hay and pea straw) on physical the yield and its components, of oyster mushroom (Pleurotus florida) fruit bodies. These parameters were measured during the experiment of Cap diameter (cm), Cap weight (gm), fruit body weight (gm), dry matter, early yield (gm), total yield (gm), NPK contents, carbohydrate content and protein content. The results revealed that, there were significant differences in all characters under study, the use of rice straw has achieved the highest results in most of these characters. With regard to chemical constituents of fruit bodies, using rice straw substrate enhanced the percentages of dry matter, phosphorus, potassium and total carbohydrates. On the other hand, the bena straw, pea straw and alfalfa hay were the highest values in nitrogen and crude protein. In generaly the results indicated that, the suitable substrates used for cultivation and production of oyster mushroom was rice straw of the substrate as compared to other substrates.

Effect of Chemically Disinfected Wheat Straw on the Growth and Yield of Pleurotus Ostreatus Mushroom

We investigated the effect of chemically disinfected wheat straw on the growth and yield of oyster mushroom (Pleurotus ostreatus). Various levels of treatments combined with different time (12, 18 and 24 hours) and dose of Formalin (250, 500 and 750ppm) and Bavistin (75ppm) were used, and the results were compared with the control. Compared to the control, almost all the treatments showed increased values, and among them significantly higher mycelium running rate, the lowest time from stimulation to primordial initiation and to harvest, number of primordial per packet, number of fruiting body per packet were found in treatment consists of 750ppm of Formalin with 75ppm of Bavistin for 18 hours. Length and diameter of the stipe, diameter and thickness of pileus, biological yield, economic yield, dry yield and biological efficiency were also significantly increased in this treatment. Wheat straw treated with 500ppm of Formalin with 75ppm of Bavistin for 18 hours performed best in terms of per cent protein and nitrogen content. However, weight of individual fruiting body was significantly higher when treated with 250ppm Formalin with 75ppm of Bavistin for 24 hours.As the sterilization of wheat straw using 750ppm of formalin with 75ppm of Bavistin for 18 hours could control pathogens and gave increased yield, this treatment might be useful as an economically effective treatment for the highest yield of oyster mushroom.

Yield and Some Macro-Morphological Characters of Pleurotus pulmonarius (Fries) Quel. Fruit Bodies Cultivated on HCl-Optimized Oil Palm Bunch Substrate

This study was conducted to study the yield and some macro-morphological characters of Pleurotus pulmonarius fruit bodies cultivated on Hydrochloric acid (HCl) optimized oil palm bunch (OPB) substrate. Concentrated HCl was diluted in tap water at 0.1%, 0.2%, 0.3%, 0.4% and were used to induce changes on the initial pH (9.5) of OPB to 8.9, 8.2, 7.9, 6.2 and control (9.1) respectively; after soaking for 48 hrs. One way Analysis of variance (ANOVA) and Correlation test were adopted for data analysis. Mean separation was also done by Duncan Multiple Range Test (DMRT) at probability level of 5%. Results showed that 0.1%, 0.2%, 0.3% and 0.4% HCl treated OPB substrates produced P. pulmonarius primordia after 9, 9, 10, 11 and control (12 days) respectively. Results further revealed that 0.4% HCl treated OPB substrate induced the highest (900 g/kg) fruit body yield and Biological Efficiency (90%) while control (493 g/kg and B.E 49.3%) respectively, produced the lowest quantity of fruit bodies. Some macro-morphological characters of harvested fruit bodies revealed that mean cap size (C.Scm) and Weight (wt.g/kg) of fruit bodies were highest (3.83 cm and 3.5 g/kg) in 0.4% HCl treated OPB respectively. Mean Stipe Length (S.Lcm) was highest (2.77 cm) in 0.3% OPB substrate and was significant at p ≤ 0.05. S.L and C.S of fruit bodies as well as C.S and Wt. were significantly correlated while there was no correlation between S.L and Wt. of fruit bodies. HCl was found as a suitable acid buffer for the optimization of the pH of the highly alkaline OPB for cultivation of P. pulmonarius fruit bodies. Oil palm bunch should therefore be adopted in the commercial production of the Oyster mushroom if certified safe for human consumption.

Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium

&lt;p class="IsiAbstrakIndo"&gt;&lt;em&gt;&lt;span lang="EN-GB"&gt;Pleurotus ostreatus&lt;/span&gt;&lt;/em&gt;&lt;span lang="EN-GB"&gt; cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of &lt;/span&gt;&lt;em&gt;&lt;span lang="EN-GB"&gt;P. ostreatus&lt;/span&gt;&lt;/em&gt;&lt;span lang="EN-GB"&gt; on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating &lt;/span&gt;&lt;em&gt;&lt;span lang="EN-GB"&gt;P. ostreatus&lt;/span&gt;&lt;/em&gt;&lt;span lang="EN-GB"&gt; in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.&lt;/span&gt;&lt;/p&gt;

Bioprospecting of powdered pineapple rind as an organic supplement of composted sawdust for Pleurotus ostreatus mushroom cultivation.

Pineapple rind is a by‐product of the pineapple processing industry and contains nutrients and other compounds which must be utilized as a bioresource for socio‐economic benefits while preventing the potential problems of improper agroindustrial biomass disposal methods. Pleurotus ostreatus is an edible oyster mushroom with medicinal properties and can be cultivated on various agroindustrial biomass, including sawdust containing supplements. Pineapple rind was powdered and used as a supplement of composted sawdust at 2%, 5%, 10%, 12%, 15%, and 20% (w/w) on dry weight basis. A control treatment consisted of composted sawdust supplemented with rice bran at 12% (the most utilized composition in Ghana). P. ostreatus strain EM‐1 was cultivated on these treatments. Factors investigated included the spawn run period, yield, fruiting body weight and size, biological efficiency, and nutritional composition (proximate composition and Copper, Zinc and Lead content) of fruiting bodies harvested from selected high‐yielding treatments and the control treatment. Full colonization of all treatments occurred by the 34th day of incubation. Enhanced yield, fruiting body weight and size, and biological efficiency were generally recorded with supplementation at lower concentrations (2% and 5%) compared to treatments supplemented at higher concentrations. There was also a supplement concentration‐dependent alteration of the nutritional composition of the mushroom. Powdered pineapple rind can be utilized as an organic supplement at relatively low concentrations in composted sawdust for P. ostreatus strain EM‐1 cultivation. The use of lower concentrations of powdered pineapple rind in composted sawdust is advantageous as relatively less input will be required to produce higher P. ostreatus strain EM‐1 yields. Utilization of pineapple rind for mushroom cultivation will extend the pineapple plant value chain, intensify mushroom production in a sustainable way, and minimize agricultural losses.

Efek Ketebalan Casing dan Ketebalan Media Terhadap Hasil Jamur Merang (Volvariella volvaceae)

The mushroom in the Acehnese language is called the bowl/addy straw mushroom (Volvariella volvaceae) including the most well known fungi among several species of mushrooms cultivated in Indonesia. The purpose of this research is to study the effect of the thickness of the casing and the composition of the media on the yield of mushroom (Volvariella volvaceae) using a Factorial Randomized Design with three replications. The first factor is the composition of the media and the second factor is the thickness of the casing. Data analysis was done by using SAS V9.12 software and advanced test with Duncan test at 5% level. This experiment showed that there is a significant effect on the interaction between the treatment of the media composition and the thickness treatment of the casing (K1C1 and K0C1) on the head body weight per harvest period, and there is no interaction with other variables. While the composition of the media singularly significant effect on the length of the fruit body and the number of fruit body in the treatment of the composition of oil palm empty bunches + lime + bran + cornflour (K1). In other variables, although statistically not yet showing the difference, but on average it appears that the medium of oil palm empty bunches + lime + bran + cornflour (K1) has the frequency of harvest, fruit body diameter, fruit body length and total body weight and fruit weight is higher than the treatment others. While the average appearance of mushroom pin head of the highest value is in treatment (K0). Average treatment without casing (C0) in addition to affect the body length of the fruit (fruting body) also gives the highest value on the appearance of pin head (going to fruit body), harvest frequency, body diameter. While the appearance of the pin head, the number of fruit body and body weight of mushroom fruit perang has the highest value on the 5 cm thickness casing treatment (C1)

Characterization of Polysaccharides from the Fruiting Bodies of Two Species of Genus Ganoderma (Agaricomycetes) and Determination of Water-Soluble β-D-Glucan Using High-Performance Liquid Chromatography.

Molecular weight (Mw) distributions of polysaccharides from the fruiting bodies of different Ganoderma lucidum strains and G. sinense were investigated and compared using high-pressure size exclusion chromatography/multiangle laser light scattering/refractive index analysis. Results showed that there were big differences in the Mw distributions and characteristics of polysaccharides from 2 species of Ganoderma. All tested G. lucidum materials exhibited similar polysaccharide distributions and similar characteristics for each fraction. The fraction with highest Mw (peak 1) was identified as β-(1→3)-linked D-glucan with (1→6)-β-D-glucopyranosyl side branches. G. sinense fruiting bodies did not include the β-D-glucan when compared with G. lucidum. A high-pressure size exclusion chromatography method was developed and applied to determine the amount of high-Mw β-D-glucan in G. lucidum fruiting bodies. Results indicated that there was no obvious relationship between β-D-glucan content and the genetic similarity of G. lucidum. The strain labeled "Longzhi no. 2" was determined to possess the largest amount of β-D-glucan: 8.2 mg/mL based on the dry weight of fruiting bodies. The β-D-glucan content in the hot water extract of Longzhi no. 2 reached 17.05%. For the "Hunong no. 1" strain, the β-D-glucan content in log-cultivated fruiting bodies was much higher than that in bag-cultivated ones. This method could be used to improve quality control of polysaccharides in G. lucidum.

KAJIAN MACAM LIMBAH DAN PENAMBAHAN TEPUNG TONGKOL JAGUNG TERHADAP PERTUMBUHAN DAN HASIL JAMUR TIRAM (Pleurotus ostreatus)

&lt;span class="fontstyle0"&gt;One limiting for factor the production of oyster mushrooms was difficult to obtain the raw material of sengon wood sawdust, meanwhile, the production of oyster mushroom necessary need the nutrients in the form of bran or cornmeal. The purpose of this study was to study planting medium, the concentration of corn cob flour, and to find the effective interaction of these two factors on growth and yield of oyster mushroom. This study used a completely randomized design (CRD) with two factors, concentration of media and corn cob flour. The media used in this study are sengon sawdust, glugu sawdust, acacia wood sawdust, rice straw and bagasse, while the concentration of corn cob flour was 0% per baglog, 1% per baglog, 2% per baglog, 3% per baglog and 4% per baglog. Data analysis was using F test level 5% and continued with Duncan test. The results of this study showed that sawdvst sengon media generally give better effect to the growth and yield of oyster mushroom, while corn cob flour treatment concentration was not known exactly in enhancing the growth and yield of oyster mushroom. Treatment of media accelerate the deployment of mycelium old, when appearing pin head, increasing the number of fruiting bodies in a single clump and increasing the mushroom fruit body weight. Extra flour treatment corncob accelerate as emerging pin head, increasing the number of fruiting bodies in a clump and increase total body weight of mushrooms.&lt;/span&gt;

Effect of Different substrate Disinfection Methods on the Production of Pleurotus ostreatus

AbstractThe aim of this paper is to estimate which of disinfection method is more effective in case of higher yield and fewer cost in cultivation of Pleurotus ostreatus in rural areas. For this purpose, four different treatments; scalping in hot water (60, 80 and 100°C), and chemical disinfection (1% formaldehyde) were compared with sterilization by autoclave. It has been noticed that method of disinfection affects mycelium growing duration(day), yield(g/kg), biological efficiency (BE%), avarage weight of fruitbody(g), diameter of pileus(cm), stipe lenght(cm) and width(cm). P. ostreatus took significantly much time to colonise the substrate (19.9 days) disinfected by chemical disinfection compared with others. Chemical disinfection (271.28 g/kg and 91.96%) and scalding hot water at 80oC (263.42 g/kg and 90.83%) had highest productivity in terms of yield and BE, respectively, while scalding hot water at 60oC (136.08 g/kg and 46.92%) had least productivity. The largest diameter of fruitbody was obtained by scalding in hot water at 80oC, while the longest stipes were harvested in disinfected substrate by chemicals. In conclusion, cheaper and more easily applecable scalping in hot water 80°C and chemical disinfection may be use for the disinfection of growing substrates instead of sterilization with autoclave in rural areas.Keywords: mushroom growing substrate, disinfection methods, autoclaved, scalding, chemical disinfection, Pleurotus ostreatus

Evaluation of Substrate Components on Shiitake Mushroom Properties

ABSTRACTShiitake mushroom [Lentinus edodes (Berk.)] can have an important role in specialty agriculture where land is limited. This research was the first step to improve mushroom cultivation technology in Iran using locally available lignocellulosic materials as substrate and supplemental materials. Effects of lignocellulosic substrate and supplemental material on biological efficiency, spawn running period, average weight and number of fruiting bodies, and yield of shitake mushroom was determined. Lignocellulosic sources used were oak sawdust, maple sawdust, fir sawdust, and wheat straw, and supplemental materials added were wheat bran, rice bran, maize powder, and no supplemental material (control). The highest biological efficiency (92.35%) was obtained with oak as the lignocellulosic material. The highest fruiting body weight (33.51 g) was noted where rice bran was used as a supplement. Spawn running period was reduced 29.82% with oak sawdust compared to fir sawdust or wheat straw. The highest and lowest numbers of fruiting bodies were from oak sawdust and wheat straw, respectively. There were no differences in yield between rice bran and the control. Substrate had a more pronounced effect on shiitake mushroom culture than did supplemental materials. The best substrate was oak sawdust and the best supplemental material was wheat bran.

Characteristics of interspecific hybrids between Pleurotus ostreatus and P. eryngii

In this study, three species of oyster mushroom (Pleurotus osturatus, P. eryngii and P. cornucopia) were crossed together in order to aggregate benefit special attributes to the genotype (s). The monokaryon of each of these species was prepared. Then, the monokaryons of two species were placed at 5 mm distance from each other to produce dikaryon. The results showed that, from among 700 crosses, only the crosses of eryngii and osturatus monokaryons were grown toward each other and produced clamps (dikaryons). Four produced hybrids were noted H1, H32, H11 and H40. The spans of produced hybrids were prepared; then, they were grown into sterile media and attributions of hybrids were studied. The results indicated that H11 hybrid was an appropriate hybrid in terms of the number of days until growing, number of days until the observation of the first pin and the days from planting to harvest. However, H40 was the best hybrid based on cap diameter, dry and fresh weight of fruit body, yield and biomass. It is expected that these interspecific hybrids employ for future oyster mushroom breeding programs.

Effect of ozone gas as an elicitor to enhance the bioactive compounds in Ganoderma lucidum

The bioactive compounds of Ganoderma lucidum are used for various human ailments resulting in a high demand for G. lucidum across the globe. The cultivation and isolation of bioactive compounds is not sufficient to balance the supply and current demand. Therefore, this study aims to increase the amount of bioactive compounds by subjecting this mushroom to one of the abiotic elicitors, ozone. The fresh fruiting bodies were exposed to ozone gas at different concentrations (1, 5 and 9μLL−1) and time intervals (1, 12, 24 and 48h) to evaluate the physical as well as the chemical changes induced to the fruiting bodies. Physical parameters of the study include colour, weight, and firmness of fruiting bodies, whereas the chemical measures include detection of total phenolic content, flavonoids, water-soluble polysaccharides, and crude ganoderic acids. The antioxidant properties of fruiting bodies were determined by DPPH, FRAP and ABTS assays.

Effect of local substrates on the growth and yield of Pleurotus ostreatus K. in the North West Region, Cameroon

Food security to the ever growing world population is a major challenge of the 21 st century. Scientists all over the world are seriously exploring ways and means to bring more food on the table. Cultivation of highly nutritional and medicinal mushrooms on local substrates is one of such effort. In an attempt to bring in our own contribution, locally available substrates for the cultivation of Pleurotus ostreatus was investigated at the Mbeng-Adio mushroom cultivation center Banjah Bamenda, Cameroon from March to September 2015. Pleurotus ostreatus was cultivated on different supplemented substrates such as Corn cobs on corn flour T1, Corn cobs on rice bran T2, Iroko on corn flour T3, Iroko on rice bran T4, Eucalyptus on corn flour T5 and Eucalyptus on rice bran T6; supplemented with 1% CaCO3. The experiment was laid in a completely randomized design. The effects of various substrates on comparative growth and yield performance of oyster mushroom were then analyzed. The highest degree of colonization after inoculation (95%) and lowest time from primordia initiation to harvest (3.25days) were obtained in T6. The highest biological yield (0.47 kg/packet), economic yield (0.43 kg/packet) and 75% contamination were obtained with T1. Highest fresh weight (0.47 kg/packet), highest dry weight(0.09 kg/packet), highest average number of primordia/packet(226.3), highest average number of fruiting body/packet(43.25), the highest average weight of individual fruiting body(0.02 kg), highest mean thickness of stipe (12.08) and the highest average number of effective fruiting body/packet(26.25) were obtained in T5. Among many aspects, T5 was found as the best substrate with biological yield (0.47 kg/packet) and economic yield (0.43 kg/packet) followed by T1, T6, T4, and T3, with T2 rejected due to 100% contamination for the production of the oyster mushroom.

Nghiên cứu môi trường thích hợp cho sản xuất quả thể nấm dược liệu Cordyceps militaris (Clavicipitaceae: Hypocreales)

Medicinal mushroom, Cordyceps militaris is an entomopathogenic fungus with many medicinal values similarly to Cordyceps sinensis and has been used for a long time in traditional medicines. Cordyceps militaris is able to grow and develop on artificial media. This study was aimed at examining the impact of nutritive ingredients on the growth of Cordyceps militaris to optimize the medium for fruiting body (sporocarp) and cordycepin production. The results showed that SDAY1 and SDAY3 media were suitable for the growth of Cordyceps militaris fungus; the medium of high protein white rice (ST20) added with 3% glucose, 1% yeast extract, 1% peptone, 0.01% kitin and vitamins B1, B12 was optimum where yielded fruiting body length from 5.82 to 7.94 cm at 60 days after culture, fresh fruiting body weight of 16.52±0.09 g and the cordycepin content in fruiting bodies of 5.56 mg/g.