Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium

Aris Mumpuni,Endang Sri Purwati,Purnomowati Purnomowati,Nuraeni Ekowati

doi:10.15294/biosaintifika.v9i3.11660

Aris Mumpuni, Endang Sri Purwati + Show 2 more

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https://doi.org/10.15294/biosaintifika.v9i3.11660

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Abstract

Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.

Highlights

The nutrient content of fruitbody and mushroom mycelia is influenced by the constituent material of growth media, which is an important aspect to determine the quality of mushroom fruit body
As the three types of medium used contain different nutrients, it is possible that the stationary phase were not be achieved at the same time. This resulted in difference of weight of harvested mycelia, either fresh or dry (Table 2 and Figure 2 A and B)
The highest weight of mycelia was ob

Summary

Introduction

Ex.Fr) Kummer or white oyster mushroom can grow on lignin and cellulose containing medium and so is found in weathered woods. People have been widely cultivating P. ostreatus as edible mushroom. It is charactized by high nutritional contents of 10.5-30.4% protein, 1.6-2.2% fat, 57.6-81.8% carbohydrates, 7.5-8,7% fiber, and 6.1-9.8% ash (Rashad et al 2009). P. ostreatus has medicinal properties through its bioactive compounds isolated from fruitbody that showed good activity in inhibiting the proliferation of cervix cancer cells (HeLa) (Ekowati et al, 2017). The nutrient content of fruitbody and mushroom mycelia is influenced by the constituent material of growth media, which is an important aspect to determine the quality of mushroom fruit body

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