Weak Barrier Research Articles

Size-dependent leak of soluble and membrane proteins through the yeast nuclear pore complex.

Nuclear pore complexes (NPCs) allow selective import and export while forming a barrier for untargeted proteins. Using fluorescence microscopy, we measured in vivo the permeability of the Saccharomyces cerevisiae NPC for multidomain proteins of different sizes and found that soluble proteins of 150 kDa and membrane proteins with an extralumenal domain of 90 kDa were still partly localized in the nucleus on a time scale of hours. The NPCs thus form only a weak barrier for the majority of yeast proteins, given their monomeric size. Using FGΔ-mutant strains, we showed that specific combinations of Nups, especially with Nup100, but not the total mass of FG-nups per pore, were important for forming the barrier. Models of the disordered phase of wild-type and mutant NPCs were generated using a one bead per amino acid molecular dynamics model. The permeability measurements correlated with the density predictions from coarse-grained molecular dynamics simulations in the center of the NPC. The combined in vivo and computational approach provides a framework for elucidating the structural and functional properties of the permeability barrier of nuclear pore complexes.

A Survey on Barrier Coverage Problem in Directional Sensor Networks

Barrier coverage guarantees to detect any intruder attempting to cross the barrier of sensor networks. In the majority of studies on barrier coverage using wireless sensor networks, sensors are assumed to have an isotropic sensing model. However, in certain applications like monitoring a region using video cameras, the sensors have directional sensing model. The nature of directional sensor networks demands novel algorithms and solutions. In directional sensor networks, this paper starts from the concept and characteristics of a directional sensing model, and then summarizes the sensing properties and behaviors of directional sensors. In particular, we classify the existing research results into different categories, such as strong barrier and weak barrier, 1-barrier and k-barrier, worst and best-case coverage and exposure path coverage, any-view coverage and full-view coverage, and overview each category in terms of problem definition, assumption, usage, solution, and performance. Finally, the open research problems to be solved and future work are also discussed.

Hierarchical titania nanostructures prepared with focused ion beam-assisted anodisation of titanium in an aqueous electrolyte

Titania nanostructures have been prepared by anodisation in aqueous solution assisted by focused ion beam (FIB) milling. The structures formed are bi-periodic, a disordered “native” nanotube array, with characteristics similar to those formed by the standard anodisation process and an ordered array of tubes with larger diameters, guided by the positioning of the FIB concave pits. Low kV EDX analysis shows implanted Ga in FIB-treated titanium which is efficiently removed by the anodisation process. Following thermal annealing, the FIB-treated regions also crystallise to the same anatase phase as the native regions. This result is in stark contrast to previous FIB-assisted anodisation studies which only produced nanostructured arrays of native dimensions. This singularity is discussed in terms of the stable FIB-induced crystalline defects which, in an aqueous electrolyte, can result in the growth of a weaker barrier layer and larger tubes. This novel process gave hexagonal and square arrays with tailored cross-sectional dimensions and therefore has potential for the synthesis of novel meta-materials.

The Role Of Organizational Mechanisms In Preventing Leakage Of Unpatented Knowledge

Firms need to protect their proprietary knowledge assets from imitation in order to appropriate rents from them. A significant volume of scholarly work is devoted to legal mechanisms for intellectual property (IP) protection such as patents. However, there is a dearth of work on how firms can protect their knowledge that is not patented, such as valuable knowledge that is not codified, is inchoate, or otherwise does not meet the criteria for patenting. In this paper we explore organizational processes and mechanisms that firms can use to protect such valuable knowledge from imitation. We explore these mechanisms using interview and survey data from captive R&D centers of multinational firms in India, where the weak IP regime places weak barriers to knowledge leakage.

Fine-scale environmental variation contributes to introgression in a three-species spruce hybrid complex

Hybridization is common for many forest trees, where weak barriers to reproduction obscure species boundaries. We characterized the genomic structure of Picea populations comprising three species s ...

Wax layers on Cosmos bipinnatus petals contribute unequally to total petal water resistance.

Cuticular waxes coat all primary aboveground plant organs as a crucial adaptation to life on land. Accordingly, the properties of waxes have been studied in much detail, albeit with a strong focus on leaf and fruit waxes. Flowers have life histories and functions largely different from those of other organs, and it remains to be seen whether flower waxes have compositions and physiological properties differing from those on other organs. This work provides a detailed characterization of the petal waxes, using Cosmos bipinnatus as a model, and compares them with leaf and stem waxes. The abaxial petal surface is relatively flat, whereas the adaxial side consists of conical epidermis cells, rendering it approximately 3.8 times larger than the projected petal area. The petal wax was found to contain unusually high concentrations of C(22) and C(24) fatty acids and primary alcohols, much shorter than those in leaf and stem waxes. Detailed analyses revealed distinct differences between waxes on the adaxial and abaxial petal sides and between epicuticular and intracuticular waxes. Transpiration resistances equaled 3 × 10(4) and 1.5 × 10(4) s m(-1) for the adaxial and abaxial surfaces, respectively. Petal surfaces of C. bipinnatus thus impose relatively weak water transport barriers compared with typical leaf cuticles. Approximately two-thirds of the abaxial surface water barrier was found to reside in the epicuticular wax layer of the petal and only one-third in the intracuticular wax. Altogether, the flower waxes of this species had properties greatly differing from those on vegetative organs.

Weak crossability barrier but strong juvenile selection supports ecological speciation of the hybrid pine Pinus densata on the Tibetan plateau.

Determining how a new hybrid lineage can achieve reproductive isolation is a key to understanding the process and mechanisms of homoploid hybrid speciation. Here, we evaluated the degree and nature of reproductive isolation between the ecologically successful hybrid species Pinus densata and its parental species P. tabuliformis and P. yunnanensis. We performed interspecific crosses among the three species to assess their crossability. We then conducted reciprocal transplantation experiments to evaluate their fitness differentiation, and to examine how natural populations representing different directions of introgression differ in adaptation. The crossing experiments revealed weak genetic barriers among the species. The transplantation trials showed manifest evidence of local adaptation as the three species all performed best in their native habitats. Pinus densata populations from the western edge of its distribution have evolved a strong local adaptation to the specific habitat in that range; populations representing different directions of introgressants with the two parental species all showed fitness disadvantages in this P. densata habitat. These observations illustrate that premating isolation through selection against immigrants from other habitat types or postzygotic isolation through selection against backcrosses between the three species is strong. Thus, ecological selection in combination with endogenous components and geographic isolation has likely played a significant role in the speciation of P. densata.

Population structure of Serpula lacrymans in Europe with an outlook to the French population

In this study the genetic variation and population structure in a French population of the dry rot fungus S. lacrymans was investigated using 14 microsatellites markers and compared to the rest of Europe. In that comparison the French population possessed the same allelic diversity as rest of Europe. A weak geographic structuring of the genetic variation was observed across Europe, where the French isolates to some extent separated from the rest of Europe, indicating that weak barriers to gene flow exists. Eighty percent of the isolates had unique multilocus microsatellite genotypes, which corresponds to high recombination and dispersal by sexual spores. Deviations from Hardy-Weinberg expectations were observed in multiple loci. In most loci there was an excess of heterozygotes, which could be due to either non-random mating, presence of more than two nuclei in the secondary mycelia or another unrecognized process. A total of six vegetative compatibility (VC) groups were present in Europe, out of which four were sampled in France. One VC group was over-represented in France while two others were underrepresented, as compared to the rest of Europe.

Population structure of Serpula lacrymans in Europe with an outlook to the French population.

In this study the genetic variation and population structure in a French population of the dry rot fungus S. lacrymans was investigated using 14 microsatellites markers and compared to the rest of Europe. In that comparison the French population possessed the same allelic diversity as rest of Europe. A weak geographic structuring of the genetic variation was observed across Europe, where the French isolates to some extent separated from the rest of Europe, indicating that weak barriers to gene flow exists. Eighty percent of the isolates had unique multilocus microsatellite genotypes, which corresponds to high recombination and dispersal by sexual spores. Deviations from Hardy-Weinberg expectations were observed in multiple loci. In most loci there was an excess of heterozygotes, which could be due to either non-random mating, presence of more than two nuclei in the secondary mycelia or another unrecognized process. A total of six vegetative compatibility (VC) groups were present in Europe, out of which four were sampled in France. One VC group was over-represented in France while two others were underrepresented, as compared to the rest of Europe.

Concurrent atomistic–continuum simulations of dislocation–void interactions in fcc crystals

Dislocation interactions with distributed condensed vacancy clusters in fcc metals were simulated via a concurrent atomistic–continuum method. Due to void strengthening, the dislocation lines are found to bow as a result of pinning on the original glide plane and undergo depinning through drawing out screw dipoles and forming prismatic loops on the secondary slip plane. We discovered an inertia-induced transition between Hirsch looping and void shearing mechanisms as the void spacing ranges from the scale of nm to hundreds of nm. Contrary to prior understanding, simulations suggest that large voids (∼5nm in diameter) can behave as weak barriers to dislocation motions under high strain-rate dynamic conditions.

Complexity of and algorithms for the manipulation of Borda, Nanson's and Baldwin's voting rules

We investigate manipulation of the Borda voting rule, as well as two elimination style voting rules, Nanson's and Baldwin's voting rules, which are based on Borda voting. We argue that these rules have a number of desirable computational properties. For unweighted Borda voting, we prove that it is NP-hard for a coalition of two manipulators to compute a manipulation. This resolves a long-standing open problem in the computational complexity of manipulating common voting rules. We prove that manipulation of Baldwin's and Nanson's rules is computationally more difficult than manipulation of Borda, as it is NP-hard for a single manipulator to compute a manipulation. In addition, for Baldwin's and Nanson's rules with weighted votes, we prove that it is NP-hard for a coalition of manipulators to compute a manipulation with a small number of candidates.Because of these NP-hardness results, we compute manipulations using heuristic algorithms that attempt to minimise the number of manipulators. We propose several new heuristic methods. Experiments show that these methods significantly outperform the previously best known heuristic method for the Borda rule. Our results suggest that, whilst computing a manipulation of the Borda rule is NP-hard, computational complexity may provide only a weak barrier against manipulation in practice. In contrast to the Borda rule, our experiments with Baldwin's and Nanson's rules demonstrate that both of them are often more difficult to manipulate in practice. These results suggest that elimination style voting rules deserve further study.

Current-Phase Relation in FSIFS Josephson Junction

In the framework of Tsukada-Furusaki formula, we calculate dc josephson current at a ferromagnetic superconductor–insulator–ferromagnetic superconductor (FSIFS) junction as function of phase difference between two ferromagnetic superconductors (FSs). We consider identical FSs which are in three different Cooper pairing states: one singlet S wave pairing and two triplet with parallel and anti parallel spins. We find a sinusoidal form for strong barrier strength for above-mention Cooper pairing states while we find a deviation from sinusoidal form for weak barrier strength. Different behaviors of I(ϕ) in terms of FS exchange field can help us distinguish the type of Cooper pairing state at the FSs which is still a controversial issue in low-temperature physics.

Anthropogenic hybridization between endangered migratory and commercially harvested stationary whitefish taxa (Coregonus spp.).

Natural hybridization plays a key role in the process of speciation. However, anthropogenic (human induced) hybridization of historically isolated taxa raises conservation issues. Due to weak barriers to gene flow and the presence of endangered taxa, the whitefish species complex is an excellent study system to investigate the consequences of hybridization in conservation. We focused on three naturally reproductively isolated whitefish taxa in Germany: the endangered, anadromous North Sea houting (NSH) and Baltic houting (BH), which were reintroduced after local extinction, and the commercially stocked European whitefish (EW). To evaluate the genetic integrity of each taxon, source and reintroduced populations of NSH and BH, and EW populations were characterized based on two mitochondrial and 17 microsatellite loci. Additionally, we investigated gill raker counts as an adaptive phenotypic trait. Even though clear genetic and phenotypic differentiation confirmed the houtings as separate evolutionarily significant units, admixture analyses revealed an extensive hybrid zone. Hybridizations were introgressive, positively correlated with genetic diversity, and were reflected in the gill raker counts. The BH distribution range showed higher heterogeneity and stronger admixture than the NSH range. Erroneous stocking with non-native genotypes best explained these patterns, which pose challenges for the conservation of the endangered NSH and BH.

Propagation of Singularities for Weak KAM Solutions and Barrier Functions

This paper studies the structure of the singular set (points of nondifferentiability) of viscosity solutions to Hamilton-Jacobi equations associated with general mechanical systems on the n-torus. First, using the level set method, we characterize the propagation of singularities along generalized characteristics. Then, we obtain a local propagation result for singularities of weak KAM solutions in the supercritical case. Finally, we apply such a result to study the propagation of singularities for barrier functions.

Explaining the Non-Newtonian Character of Aggregating Monoclonal Antibody Solutions Using Small-Angle Neutron Scattering

A monoclonal antibody solution displays an increase in low shear rate viscosity upon aggregation after prolonged incubation at 40°C. The morphology and interactions leading to the formation of the aggregates responsible for this non-Newtonian character are resolved using small-angle neutron scattering. Our data show a weak repulsive barrier before proteins aggregate reversibly, unless a favorable contact with high binding energy occurs. Two types of aggregates were identified after incubation at 40°C: oligomers with radius of gyration ∼10 nm and fractal submicrometer particles formed by a slow reaction-limited aggregation process, consistent with monomers colliding many times before finding a favorable strong interaction site. Before incubation, these antibody solutions are Newtonian liquids with no increase in low shear rate viscosity and no upturn in scattering at low wavevector, whereas aggregated solutions under the same conditions have both of these features. These results demonstrate that fractal submicrometer particles are responsible for the increase in low shear rate viscosity and low wavevector upturn in scattered intensity of aggregated antibody solutions; both are removed from aggregated samples by filtering.

The contribution of Anatolia to European phylogeography: the centre of origin of the meadow grasshopper, Chorthippus parallelus

AbstractAimChorthippus parallelus is one of the classic model systems for studying genetic structure and phylogeography in the Western Palaearctic. Here, we investigate the regional genetic differentiation of C. parallelus and evaluate the historical and evolutionary processes responsible for such genetic structuring, test the nature of the Turkish Straits system as a barrier to dispersal, and explore the contribution of Anatolian populations to the biodiversity of the Western Palaearctic.LocationWestern Palaearctic.MethodsWe incorporated sequence data from dense sampling of the phylogeographically important Anatolian region with both previous and newly obtained data of the nuclear fragment cpnl‐1 and the mitochondrial fragment COI–tRNALeu–COII. In total, 1049 sequences of cpnl‐1 from 33 regions were analysed to investigate the genetic diversity, genetic structuring and phylogeography of C. parallelus across its distributional range. The mtDNA region was additionally used to test whether the Turkish Straits system acts as a barrier.ResultsThe analyses revealed that not all southern refugial populations of C. parallelus have contributed equally to the post‐glacial recolonization of Europe. Four genetic clusters across the species' range were recovered: cluster A (eastern part of the Anatolian Diagonal); cluster B (western part of the Anatolian Diagonal); cluster C (Spain, Italy, southern Balkans, west part of Anatolia and Russia); and cluster D (covering the entire distributional range of the species). The Turkish Straits system has been a weak barrier to dispersal by C. parallelus, allowing gene flow from Anatolia to the Balkans.Main conclusionsThe current patterns of genetic structuring of C. parallelus were best explained by multiple expansion and contraction events. Anatolia has been well connected to the Balkans, contributing genetically to the establishment of central and northern European populations prior to the Holocene. The Anatolian refugium is suggested to be the centre of origin for Western Palaearctic C. parallelus diversity rather than a Balkan refugium.

Limits on new physics from black holes

Black holes emit high energy particles which induce a finite density potential for any scalar field $\phi$ coupling to the emitted quanta. Due to energetic considerations, $\phi$ evolves locally to minimize the effective masses of the outgoing states. In theories where $\phi$ resides at a metastable minimum, this effect can drive $\phi$ over its potential barrier and classically catalyze the decay of the vacuum. Because this is not a tunneling process, the decay rate is not exponentially suppressed and a single black hole in our past light cone may be sufficient to activate the decay. Moreover, decaying black holes radiate at ever higher temperatures, so they eventually probe the full spectrum of particles coupling to $\phi$. We present a detailed analysis of vacuum decay catalyzed by a single particle, as well as by a black hole. The former is possible provided large couplings or a weak potential barrier. In contrast, the latter occurs much more easily and places new stringent limits on theories with hierarchical spectra. Finally, we comment on how these constraints apply to the standard model and its extensions, e.g. metastable supersymmetry breaking.

The Carpathian range represents a weak genetic barrier in South-East Europe.

BackgroundIn the present study we have assessed whether the Carpathian Mountains represent a genetic barrier in East Europe. Therefore, we have analyzed the mtDNA of 128 native individuals of Romania: 62 of them from the North of Romania, and 66 from South Romania.ResultsWe have analyzed their mtDNA variability in the context of other European and Near Eastern populations through multivariate analyses. The results show that regarding the mtDNA haplogroup and haplotype distributions the Romanian groups living outside the Carpathian range (South Romania) displayed some degree of genetic differentiation compared to those living within the Carpahian range (North Romania).ConclusionThe main differentiation between the mtDNA variability of the groups from North and South Romania can be attributed to the demographic movements from East to West (prehistoric or historic) that differently affected in these regions, suggesting that the Carpathian mountain range represents a weak genetic barrier in South-East Europe.

Elements Modulating the Prion Species Barrier and Its Passage Consequences

The specific characteristics of Transmissible Spongiform Encephalopathy (TSE) strains may be altered during passage across a species barrier. In this study we investigated the biochemical and biological characteristics of Bovine Spongiform Encephalopathy (BSE) after transmission in both natural host species (cattle, sheep, pigs and mice) and in transgenic mice overexpressing the corresponding cellular prion protein (PrPC) in comparison with other non-BSE related prions from the same species. After these passages, most features of the BSE agent remained unchanged. BSE-derived agents only showed slight modifications in the biochemical properties of the accumulated PrPSc, which were demonstrated to be reversible upon re-inoculation into transgenic mice expressing bovine-PrPC. Transmission experiments in transgenic mice expressing bovine, porcine or human-PrP revealed that all BSE-derived agents were transmitted with no or a weak transmission barrier. In contrast, a high species barrier was observed for the non-BSE related prions that harboured an identical PrP amino acid sequence, supporting the theory that the prion transmission barrier is modulated by strain properties (presumably conformation-dependent) rather than by PrP amino acid sequence differences between host and donor.As identical results were observed with prions propagated either in natural hosts or in transgenic mouse models, we postulate that the species barrier and its passage consequences are uniquely governed by the host PrPC sequence and not influenced by other host genetic factors. The results presented herein reinforce the idea that the BSE agent is highly promiscuous, infecting other species, maintaining its properties in the new species, and even increasing its capabilities to jump to other species including humans. These data are essential for the development of an accurate risk assessment for BSE.

Photovoltaic performance improvement of dye-sensitized solar cells through introducing In-doped TiO2 film at conducting glass and mesoporous TiO2 interface as an efficient compact layer

In-doped TiO2 thin film was introduced at the interface of fluorine-doped tin oxide (FTO) substrate and mesoporous TiO2 film by spin-coating method, and its application as a new compact layer material for dye-sensitized solar cells (DSSCs) was investigated. The scanning electron microscopy (SEM), UV-visible spectroscopy, current-voltage characteristics, Mott-Schottky analysis, electrochemical impedance spectroscopy (EIS) analysis and open-circuit voltage decay (OCVD) technique are used to characterize the morphology, optical transmittance and flat-band potentials (Vfb) of In-doped titania compact film and its effect to the photoelectron conversion process. It was found that In-doping increased the transmittance of TiO2 compact layer, the interfacial resistance between FTO substrate and porous TiO2 film and the flat-band potential of TiO2 film. The In-doped TiO2 compact layer effectively suppressed the charge recombination from FTO to the electrolyte, increased the optical absorption of dye and then increased the short-circuit photocurrent density (Jsc). Furthermore, In-doped TiO2 compact layer acted as a weak energy barrier, which increased the electron density in the mesoporous TiO2 film, thus improved open-circuit photovoltage (Voc). As a result, the overall energy conversion efficiency of the DSSC with In-doped TiO2 compact layer was enhanced by 11.9% and 6.9% compared to the DSSC without compact layer and with pure TiO2 compact layer, respectively. It indicated that In-doped TiO2 is a promising compact layer material for dye-sensitized solar cells.