Erosion on agricultural soils in Czech Republic threats seriously their production and nonproduction functions. Erosion have devastating effect on fertile topsoil of agricultural soils, reduces thickness of soil profile and nutrients and humus content, deteriorate physical, chemical and biological properties of soil. At Brno University of Technology in Czech Republic was designed and realized equipment the soil erosion bridge, which allows soil surface profile measurement and quantifies volume of erosion rills, which can occur after heavy rainfall. The main objective was to develop a method for volumetric quantification of erosion rills during heavy rainfall season. Using new equipment soil surface profile was measured directly in field during 4 years (2007–2010). New equipment consists of three parts. The first equipment is a square frame with an inside dimension of 4 m2. The second equipment is removable profile, which serves for movement of soil erosion bridge. The third equipment is soil erosion bridge. The soil erosion bridge serves to volumetric quantification of rill erosion. The more effective way of data processing was developed. It was developed a software, by which it is possible automatically transfer rill surface profile to digital form. In South Moravian Region in Czech Republic was selected case study area with typical very steep sloped relief with loess soil. The measurement was carried out on research plots with a slope of more than 10%. More than 1300 cross sections of soil surface profile were measured using new type of soil erosion bridge. From these profiles it was possible to calculate volume of eroded soil from research plots. The results were always in excess of 100 t.ha–1.rok–1. This is a value many times higher than tolerated limit of soil loss in Czech Republic. Thanks to this new equipment, it is possible to quantify soil loss from plot threatened of water erosion. The advantage of this equipment is its using during vegetable season when soil surface is overgrown with vegetation and crops are higher growth.