Peak Wavenumber Research Articles

Vibrational studies of polar aprotic molecule in aromatic chemical and isotropic solvents: experimental and quantum chemical investigations

Raman spectroscopic technique has been used to study the intermolecular interactions and dynamics of SO, C―H and CSC stretching modes of dimethyl sulfoxide (DMSO) in binary mixtures using methyl benzene (MBN) and deuterated methyl benzene (MBNd) aromatic solvents. The Raman band of SO stretching mode has been deconvoluted into four distinct bands for neat DMSO as well as in binary mixtures. Deconvoluted bands in neat DMSO were assigned as monomer, cyclic out‐of‐phase, cyclic in‐phase and chain dimers having peak wavenumbers 1069.10, 1056.60, 1041.50 and 1027.30 cm−1 respectively. Peak wavenumber of SO stretching mode shows red shift, while peak wavenumbers of C―H and CSC stretching modes show blue shift with the increase in solvent concentration. The vibrational relaxation phenomena for all the stretching modes have been studied as a function of solvent concentration. Quantum‐chemical calculations have been carried out to gain more insight into the self‐association of DMSO and in interacting environment with the solvents using ab initio and density functional theory method. The ab initio basis set is HF/6‐31 + G (d, p) for the interacting system. The hydrogen bond complexes of DMSO with MBN and MBNd using IEF‐PCM model have been calculated using B3LYP functional and 6‐31 + G(d,p) basis sets. Theoretical calculations have been compared with the experimental findings and we obtained good coherence of the results. Copyright © 2015 John Wiley & Sons, Ltd.

Raman study on a bulk-heterojunction film of pentacene and C60

We measured the 785-nm excited Raman spectrum of a pentacene:C60 blend film. The 1462-, 492-, and 268-cm−1 bands of C60 showed broadening in bandwidth and downward shifts in peak wavenumbers, whereas such broadening and downward shifts were not observed for pentacene. These results indicate that there are pentacene crystallites among the amorphous C60. The intensity ratio of the 1598-cm−1 (B3g) and the 1534-cm−1 (Ag) bands indicates that pentacene molecules have standing orientations. The appearance of a 514-cm−1 band (F1u) and the splitting of the Hg bands of C60 indicate symmetry lowering induced by the interaction between pentacene and C60 molecules.

Experimental and Theoretical Study of the Raman Spectra of Ammonium Thiosulfate Solution

The Raman spectra of ammonium thiosulfate solution have been experimentally measured and several typical Raman peaks of S 2 o 3 2 − in ammonium thiosulfate solution were observed. We found that there is a linear relationship between the concentration of S 2 o 3 2 − and the corresponding intensity and area of the Raman peaks. To study the possible effect of intermolecular hydrogen bonds on the vibration of S 2 o 3 2 − , its Raman spectra were calculated using the Hartree–Fock Hamiltonian with the 6-31+G(d) basis set in the gas phase, hydrated phase (simulating intermolecular hydrogen bonds by adding two water molecules), and by the self-consistent reaction field model (SCRF). The study shows that in the gas and hydrated phases, the wavenumbers of peaks in the Raman spectra are in good agreement with the experimental values in the low-frequency region, and there is a red shift in the highfrequency region. In contrast, in both the low and high-frequency regions, the wavenumbers in the SCRF model are closer to the experimental values than those in the former two phases. This shows that the SCRF model is most suitable for the calculation of the Raman spectra of S 2 o 3 2 − in ammonium thiosulfate solution.

Development of time-domain differential Raman for transient thermal probing of materials.

A novel transient thermal characterization technology is developed based on the principles of transient optical heating and Raman probing: time-domain differential Raman. It employs a square-wave modulated laser of varying duty cycle to realize controlled heating and transient thermal probing. Very well defined extension of the heating time in each measurement changes the temperature evolution profile and the probed temperature field at μs resolution. Using this new technique, the transient thermal response of a tipless Si cantilever is investigated along the length direction. A physical model is developed to reconstruct the Raman spectrum considering the temperature evolution, while taking into account the temperature dependence of the Raman emission. By fitting the variation of the normalized Raman peak intensity, wavenumber, and peak area against the heating time, the thermal diffusivity is determined as 9.17 × 10(-5), 8.14 × 10(-5), and 9.51 × 10(-5) m(2)/s. These results agree well with the reference value of 8.66 × 10(-5) m(2)/s considering the 10% fitting uncertainty. The time-domain differential Raman provides a novel way to introduce transient thermal excitation of materials, probe the thermal response, and measure the thermal diffusivity, all with high accuracy.

Effects of the Various Solvents on the In vitro Permeability of Indomethacin through Whole Abdominal Rat Skin

Purpose : The aim of the present study was to evaluate the effect of some solvents on in vitro dermal absorption of indomethacin. Methods: The effects of different solvents such as Plurololeique, Labrasol, Tween 80, Isopropyl myristate and PEG 400 on indomethacin permeation through pretreated rat skin were evaluated. A specially designed Franz diffusion cell was used and the data were compared to the results of hydrated abdominal rat skin as a common control. The measured permeability parameters included permeability coefficient (Kp), diffusion coefficient (D) and flux (Jss). Enhancement mechanisms were studied by comparing changes in peak wave number position and their intensities of asymmetric (Asy) and symmetric (Sym) C-H stretching, lipid ester carbonyl stretching in SC, C=O stretching (Amide I) and C-N stretching of keratin (Amide II) absorbance using Fourier transform infrared spectroscopy (FTIR). Using differential scanning calorimetry (DSC), their mean transition temperature (Tm) and enthalpies ( ∆H) were also compared. Results : All the solvent materials significantly increased flux (Jss) (p<0.05), with isopropyl myristate

Spectroscopic Characterization of Disodium Hydrogen Orthophosphate and Sodium Nitrate after Biofield Treatment

Disodium hydrogen orthophosphate is a water soluble white powder widely used as pH regulator and saline laxative. The sodium nitrate is a highly water soluble white solid, used in high blood pressure, dentinal hypersensitivity, and production of fertilizers. The present study was aimed to investigate the impact of biofield treatment on spectral properties of disodium hydrogen orthophosphate and sodium nitrate. The study was performed in two groups i.e., control and treatment of each compound. The treatment groups were subjected to Mr. Trivedi’s biofield treatment. The spectral properties of control and treated groups of both compounds were studied using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of biofield treated disodium hydrogen orthophosphate showed the shifting in wavenumber of vibrational peaks (with respect to control) corresponding to O-H stretching from 2975 to 3357 cm-1, PO-H symmetrical stretching from 2359 to 2350 cm-1, O=P-OH deformation from 1717-1796 cm-1 to 1701-1735 cm-1, P=O asymmetric stretching from 1356 to 1260 cm-1 and P=O symmetric stretching from 1159 to 1132 cm-1, etc. Likewise, the FT-IR spectrum of sodium nitrate exhibited the shifting of vibrational frequency of N=O stretching from 1788 to 1648 cm-1 and NO3 asymmetric and symmetric stretchings from 1369 to 1381 cm-1 and 1340 to 1267 cm-1. UV spectrum of treated disodium hydrogen orthophosphate revealed a negative absorbance; it may be due to decrease in UV absorbance as compared to control. UV spectrum of control sodium nitrate exhibited two absorbance maxima (λmax) at 239.4 nm and 341.4 nm, which were altered to one absorbance maxima (λmax) at 209.2 nm after biofield treatment. Overall, the FT-IR and UV spectroscopic data of both compounds suggest an impact of biofield treatment on spectral properties with respect to force constant, bond strength, dipole moments and transition energy between two orbitals (ground state and excited state) as compared to respective control.

Fourier Transform Infrared and Ultraviolet-Visible Spectroscopic Characterization of Ammonium Acetate and Ammonium Chloride: An Impact of Biofield Treatment

Ammonium acetate and ammonium chloride are the white crystalline solid inorganic compounds having wide application in synthesis and analytical chemistry. The aim of present study was to evaluate the impact of biofield treatment on spectral properties of inorganic salt like ammonium acetate and ammonium chloride. The study was performed in two groups of each compound i.e., control and treatment. Treatment groups were received Mr. Trivedi’s biofield treatment. Subsequently, control and treated groups were evaluated using Fourier Transform Infrared (FT-IR) and Ultraviolet-Visible (UV-Vis) spectroscopy. FT-IR spectrum of treated ammonium acetate showed the shifting in wavenumber of vibrational peaks with respect to control. Like, the N-H stretching was shifted from 3024-3586 cm-1 to 3033-3606 cm-1, C-H stretching from 2826-2893 cm-1 to 2817-2881 cm-1, C=O asymmetrical stretching from 1660-1702 cm-1 to 1680-1714 cm-1, N-H bending from 1533-1563 cm-1 to 1506-1556 cm-1 etc. Treated ammonium chloride showed the shifting in IR frequency of three distinct oscillation modes in NH4 ion i.e., at ν1, 3010 cm-1 to 3029 cm-1; ν2, 1724 cm-1 to 1741 cm-1; and ν3, 3156 cm-1 to 3124 cm-1. The N-Cl stretching was also shifted to downstream region i.e., from 710 cm-1 to 665 cm-1 in treated ammonium chloride. UV spectrum of treated ammonium acetate showed the absorbance maxima (λmax) at 258.0 nm that was shifted to 221.4 nm in treated sample. UV spectrum of control ammonium chloride exhibited two absorbance maxima (λmax) i.e., at 234.6 and 292.6 nm, which were shifted to 224.1 and 302.8 nm, respectively in treated sample. Overall, FT-IR and UV data of both compounds suggest an impact of biofield treatment on atomic level i.e., at force constant, bond strength, dipole moments and electron transition energy between two orbitals of treated compounds as compared to respective control.

Temperature and Hydration Dependence of Low-Frequency Spectra of Lipid Bilayers Studied by Terahertz Time-Domain Spectroscopy.

We have studied temperature and hydration dependent low-frequency spectra of lipid bilayers of 1,2-dimyristoyl-sn-glycero-3-phosphoryl-3'-rac-glycerol (DMPG) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) by terahertz time-domain spectroscopy (THz-TDS). We measured X-ray diffraction patterns and mid-infrared spectra of these lipid bilayers and found that the lipid bilayers have two different types of phases, i.e., the gel phase and the crystalline phase, depending on the preparation methods of the samples. In both phases, a few distinct bands were observed in the THz region. For DMPG, the peak wavenumbers of the absorption bands did not change upon hydration, while the bandwidth in the crystalline phase was smaller than that in the gel phase. We performed spectral analyses for the complex dielectric spectra for DMPG and DMPC with a model function, mainly to determine the peak wavenumbers of the absorption bands. In contrast to the case of the DMPG bilayers, the peak wavenumber of the absorption band of the DMPC bilayer shifts upon hydration. In the hydrated DMPC bilayer, it was suggested fast reorienting water molecules exist with a relaxation time of sub-picoseconds. It is suggested that the THz absorption patterns reflect the lipid packing pattern in the bilayers. The temperature dependence of the absorption band was analyzed by an empirical equation, and the anharmonicity of the vibrational potential of the low-frequency mode was quantitatively evaluated.

Solvation and electrochemical analyses of neodymium complexes in TFSA-based ionic liquids dissolving the nitrates synthesized from spent Nd–Fe–B magnets

In this study, rare earth (RE) nitrates, M(NO3)3·xH2O, were synthesized from Nd–Fe–B magnets in order to apply nitric acid as a low cost acid medium. The synthesized RE nitrates were identified by ICP-AES, XRD and TG-DTA. These results revealed that the RE nitrates were mainly composed of Nd(NO3)3·6H2O.In addition, the solubility of the RE nitrates in n-hexyl-trimethyl-ammonium bis(trifluoromethyl-sulfonyl)amide, [N1116][TFSA], ionic liquids (ILs) was investigated by UV–Vis–NIR and Raman spectra. According to the hypersensitive peak (4I9/2→2G7/2) of Nd3+, the solubility of the RE nitrates in [N1116][TFSA] was estimated to be 0.05moldm−3. The solvation of the neodymium complex in [N1116][TFSA] dissolved RE nitrates was also researched by UV–Vis–NIR spectra. The molar extinction coefficients and the peak wavenumbers of the neodymium complex in ILs differed from related complexes such as [Nd(TFSA)5]2−, [Nd(H2O)9]3+ and [Nd(NO3)5]2−. This result suggested that the solvated structure was [Nd(NO3)a(TFSA)5−a]2− and the bond strength between the central Nd3+ ion and the surrounding ligands was expressed in the following order: [NO3−]<[TFSA−]<H2O. However, the significant peak at 750cm−1 did not appeared in Raman spectrum of [Nd(NO3)a(TFSA)5−a]2− in [N1116][TFSA]. This result revealed that the [TFSA−] anion bound to the central Nd3+ ion in the complex: [Nd(NO3)a(TFSA)5−a]2− was unstable in [N1116][TFSA]. Therefore, the spectrochemical series would be applicable for limited conditions of [NO3−] in TFSA-based ILs.Furthermore, the electrochemical behavior of the [Nd(NO3)a(TFSA)5−a]2− complex in [N1116][TFSA] was investigated by cyclic voltammetry. A cathodic current peak for [Nd(NO3)a(TFSA)5−a]2−/Nd(0) was observed at −3.0V and this reduction potential was shifted to the more positive side compared to [Nd(TFSA)5]2−/Nd(0), so that the interaction between the central Nd3+ ion and the ligand of [Nd(NO3)a(TFSA)5−a]2− would be weaker than that of [Nd(TFSA)5]2−. The diffusion coefficient of [Nd(NO3)a(TFSA)5−a]2− in [N1116][TFSA] was calculated to be 3.5×10−13m2s−1, which is almost the same as that for [Nd(TFSA)5]2−. The series of results enabled us to demonstrate that direct dissolution from RE nitrates and the electrodeposition in a TFSA-based ILs system could be applied for the recovery process of Nd metal.

Study on Dendrobium loddigesii Rolfes from different regions and harvest periods by FTIR

Infrared Spectroscopy (IR) integrated with two dimensional correlation infrared spectroscopy (2DCOS IR) was employed to rapidly discriminate Dendrobium loddigesii Rolfes (DR) from different regions and harvesting periods. The results showed that the IR peaks around 1 035, 1 051, 1 078, 1 156, 1 500, 1 511 and 1 736 cm-1had perceptible differences among DRs from different regions, indicating that different DRs containing remarkable different compositions and contents of polysaccharides, ketones and esters. 2DCOS IR spectra of DRs from Vietnam, Yunnan, Guangxi, Guizhou each had seven, eight, eight, nine auto peaks, respectively; furthermore, DRs from Guagnxi had the strongest peak in 1 220 cm-1, which was distinguish to those of other DRs (980 cm -1). In the IR spectra of DRs from different harvest seasons, the wave number of key peaks in (1 034 approximately1 023)cm- 1, the wave number of minor peaks in (1 6174)cm-1, as well as the presence of peaks in 1 078(1 076, 1 079)cm-1, showed obvious periodic changes with the seasons, which indicated the accumulation of polysaccharides and ketones from DRs displayed an evident periodic variability discipline. The application of FTIR in DRs could facilitate acquiring their growth conditions, composition and content changes, which would be significant in rational exploitations and utilizations of DR

Electroluminescence emission of crystalline germanium nanoclusters deposited with laser assistance at low temperature.

With CO2 laser assistance, crystalline Ge nanocluster-embedded Ge films were deposited at low temperature using a conventional plasma-enhanced chemical vapor deposition system. Raman spectrum showed a wavenumber peak at 290 cm(-1) which corresponded to the crystalline Ge nanoclusters in the Ge film deposited with CO2 laser assistance. Crystalline Ge nanoclusters embedded in Ge matrices were observed from transmission electron microscopy (TEM) images and electron diffraction pattern. The electroluminescent devices constructed with multilayered Ge nanoclusters-embedded Ge films were fabricated. The experimental results demonstrated that the electroluminescence emission originated from the radiative recombination of the electron-hole pairs in the Ge nanoclusters.

Polarized IR MEIRAS Study of Surface Orientations of CO Molecules Adsorbed on Pt Nanowires Catalysts

Multilayer enhanced infrared reflection spectroscopy (MEIRAS) experiments were performed on parallel Pt nanowires nanofabricated on TiO2 and SiO2 substrates, while being exposed to 1% CO at atmospheric pressure and 75 °C. We studied the effect of nanowire widths and IR polarization and incidence angle on peak wavenumbers and intensities of absorption bands of CO molecules adsorbed on Pt surfaces. The samples consist of 4 mm × 4 mm arrays of rough polycrystalline parallel Pt nanowires placed 200 nm apart, 15–90 nm wide, and approximately 8 nm high/thick. The nanowires have somewhat straight side-faces because of the directional nature of the Pt deposition process used. We also studied 5 nm thick polycrystalline Pt film samples. Spectra from unpolarized IR at small incidence angles (20° from surface normal) showed a decrease in linearly adsorbed CO peak wavenumbers with decreasing nanowire width, from about 2090 cm –1 on Pt film to less than 2055 cm –1 on thin nanowires, indicating differences in Pt–CO inte...

Preliminary studies of the effects of psychological stress on circulating lymphocytes analyzed by synchrotron radiation based-Fourier transform infrared microspectroscopy

Psychological stress is a condition that not only generates behavioral disorders but also disrupts homeostasis and immune activity that can exacerbate or lead to inflammatory diseases. The aim of this work was to study biochemical changes in circulating immune cells from rats under psychological stress by using vibrational spectroscopy. A stress model was used, where exposure to a stressor was repeated for 5days. Subsequently, circulating lymphocytes were examined for their biomolecular vibrational fingerprints with synchrotron radiation based-Fourier transform infrared microspectroscopy. The results showed an increased absorption at the ester lipid region (1720–1755cm−1) in lymphocytes from stressed rats, suggesting lipid peroxidation. Statistical significant changes in wavenumber peak position and absorbance in the nucleic acid region were also observed (915–950cm−1 Z-DNA, 1090–1150cm−1 symmetric stretching of POC, 1200–1260cm−1 asymmetric PO2 and 1570–1510cm−1 methylated nucleotides) which suggest a reduction of transcriptional activity in lymphocytes from stressed rat. These results unravel part of the mechanisms by which psychological stress may affect the immune system leading to systemic consequences.

Solute–Solvent Interactions of Benzonitrile in Solutions Studied by Sub-Picosecond Infrared Pump–Probe Spectroscopy

Abstract The vibrational energy relaxation of the CN stretching mode of benzonitrile (BN) in solution has been investigated by infrared (IR) pump–probe spectroscopy. The peak wavenumber, line shape, and cross section of the ground state absorption band due to the CN stretching mode depend on the solvent and concentration. From the experimental results of IR spectroscopy and calculations with density functional theory, the structures of solute–solute or solute–solvent complexes are suggested. In hexane, BN exists as a monomer or a dimer in which the two CN groups are arranged antiparallel. In ethanol (EtOH), it is suggested that BN forms the antiparallel dimer and a BN–EtOH hydrogen-bonded complex. In dimethyl sulfoxide (DMSO), BN probably forms a solute–solvent complex. From the results of IR pump–probe spectroscopy, the decay time constant of the pump–probe signal shows a probe wavenumber dependence in hexane and DMSO, which indicates inhomogeneity of the microscopic environment around BN in these solvents. In EtOH, the wavenumber dependence was not observed. This suggests that the VER processes for the BN dimer and the BN–EtOH complex are accelerated from that for the monomer, and the VER times are almost identical among the two structures.

Collective and Single-Chain Correlations in Disordered Melts of Symmetric Diblock Copolymers: Quantitative Comparison of Simulations and Theory

We present a detailed comparison of simulations of disordered melts of symmetric AB diblock copolymers to predictions of the renormalized one-loop (ROL) theory. The behaviors of the structure factor S(q) and of single-chain correlations are studied over a range of chain lengths (N = 16, ..., 128) for two models: one with harshly repulsive pair interactions and another with very soft interactions. The ROL theory is shown to provide an excellent description of the dependence of S(q) on chain length and thermodynamic conditions for both models, even for very short chains, if we allow for the existence of a nonlinear dependence of the effective interaction parameter χe upon the strength of the AB repulsion. The decrease in peak wavenumber q* with increasing χe is shown to be unrelated to changes in single-chain correlations. Results for all quantities are consistent with the hypothesis that the ROL theory gives an exact description of the dominant O(N̅−1/2) corrections to RPA and random-walk predictions in the limit of infinite chain length N.

High-pressure infrared and Raman studies of polymorphism in pharmaceutical compounds: Spironolactone, Forms I and II

The infrared and Raman spectra of the two polymorphic forms, I and II, of the synthetic steroid spironolactone (C24H32O4S) have been examined under high pressures up to about 50kbar with the aid of a diamond-anvil cell. While the differences in peak wavenumbers between the two polymorphs are small, the difference in the pressure dependence is dramatic. Both forms undergo structural transformations under pressure, but over different pressure ranges.

Reactive Extraction of Saturated Aliphatic Dicarboxylic Acids with Trioctylamine in 1-Octanol: Equilibria, Model, and Correlation of Apparent Reactive Equilibrium Constants

Extraction equilibria for saturated aliphatic dicarboxylic acids, namely oxalic, malonic, succinic, and adipic acids, with trioctylamine (TOA) in 1-octanol were determined at various TOA concentrations. Using quantitative FT-IR spectra, we determined that the formation of 1:2 acid–amine complexes depends on the pKa2 value, and wavenumbers of specific peaks for the COO– of the acid–amine ion-pair complexes depend on the pKa1 value. An equilibrium model is presented that employs the mass action law and is used to determine model parameters and apparent extraction equilibrium constants (K11, K12, and K21). The extraction abilities for dicarboxylic acids depend on the pKa1 value. The typical overloading curves of TOA/1-octanol for dicarboxylic acids are given. The loadings of TOA calculated using the equilibrium model parameters and apparent extraction equilibrium constants agree with the experimental data. The apparent extraction equilibrium constants depend on the acidity of the dicarboxylic acid and the sp...

Viscous effects on the non-classical Rayleigh–Taylor instability of spherical material interfaces

The viscous and conductivity effects on the instability of a rapidly expanding material interface produced by a spherical shock tube are investigated through the employment of a high-order WENO scheme. The instability is influenced by various mechanisms, which include (a) classical Rayleigh–Taylor (RT) effects, (b) Bell–Plesset or geometry/curvature effects, (c) the effects of impulsively accelerating the interface, (d) compressibility effects, (e) finite thickness effects, and (f) viscous effects. Henceforth, the present instability studied is more appropriately referred to as non-classical RT instability to distinguish it from classical RT instability. The linear regime is examined and the development of the viscous three-dimensional perturbations is obtained by solving a one-dimensional system of partial differential equations. Numerical simulations are performed to illustrate the viscous effects on the growth of the disturbances for various conditions. The inviscid analysis does not show the existence of a maximum amplification rate. The present viscous analysis, however, shows that the growth rate increases with increasing the wave number, but there exists a peak wavenumber beyond which the growth rate decreases with increasing the wave number due to viscous effects.

The state of P Onb non-bridging oxygen and proton incorporation in binary MO·P2O5 (M = Ca, Mg) phosphate glasses

Abstract Binary x MO·(100 − x )P 2 O 5 (mol%; M = Ca, Mg) glasses were prepared using a conventional melting method, and the proton conduction mechanism in the glasses was investigated. The proton conductivities of the glasses were measured under various hydrogen (H 2 ) gas concentrations from 0 to 100 vol.% using Pt electrodes. The CaO·P 2 O 5 glasses show higher proton conductivities than MgO·P 2 O 5 ones. Fourier-transform infrared (FTIR) measurements showed that protons dissociated from H 2 gas at the Pt electrodes are incorporated into the glasses, and the proton conductivity increases as a result of an increase in the carrier (proton) concentration. There is a good linear relationship between the peak wavenumber of P O nb (O nb indicates non-bridging oxygen) and the proton conductivity measured in a 100 vol.% H 2 atmosphere. Furthermore synchrotron radiation X-ray diffraction (SRXRD) and FTIR results suggest that the π-electron of P O double bond is localized significantly in MgO·P 2 O 5 compared with CaO·P 2 O 5 glasses, which is the origin of higher proton conductivity in CaO·P 2 O 5 glasses.

Differentiation Between Anti-Epidermal Growth Factor Receptors Antibody Conjugated and Unconjugated Gold Nanoparticles Using Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy

Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) along with UV-Vis was used to distinguish between structural differences between a normal human breast cell line (MCF-12 F) and a cancerous breast cell line (MCF-7) after both being treated with conjugated gold nanoparticles and unconjugated gold nanoparticles. The MCF-7 cell line possessed a small amount of epidermal growth factor receptors (EGFR) on its cell surface whereas the MCF-12 F cell line did not. The anti-EGFR antibodies blocked the binding of epidermal growth factors to this receptor which plays an important role in the regulation of cell growth, proliferation, and differentiation. The infrared (IR) spectra for both cell lines yield several differences between normal biological samples and cancerous samples. As the malignancy of the sample increases, the peak intensity and wavenumber positions decrease. The same conclusion was drawn from thin-layers of conjugated nanoparticles and nonconjugated nanoparticles. The presence of conjugated nanoparticles increased the peak intensity of the MCF-7 cell line whereas it decreased the peak intensity for the MCF-12 F cell line. UV-Vis was used to show the presence of the anti-EGFR antibodies on the surface of the gold nanoparticles which was 5 nm red shifted compared to the gold nanoparticles solution.