Abstract An interlaboratory comparison was made of the variation in elemental concentration of leaf lettuce (Lactuca sativa L. cv. Grand Rapids) plants grown under base-line conditions for 28 days in 5 controlled-environment facilities. Two studies were conducted by each of 5 investigators using a sphagnum peat-vermiculite mix obtained from a common source. Plant tissue from all studies was collected and analyzed at 1 laboratory for 10 essential elements (P, K, Ca, Mg, Fe, Mn, B, Zn, Cu, Mo) and 10 non-essential elements (Al, Si, Ti, Sr, Ba, Na, Pb, V, Li, and Sn) by arc emission spectrography. The 10 essential elements occurred at concentrations adequate for normal lettuce growth. Tissue analysis data showed large differences in elemental concentration among experiments conducted by the same investigator as well as by different investigators suggesting inadvertent contamination of the leaf samples by the rooting media or contamination of the watering system in each controlled-environment facility. These differences, however, had no observable effect on vegetative growth. Based on a nested analysis on variance, 95% of the elements showed significant plant to plant differences in concentration. However, based on an analysis of variance components, the greatest source of variation was found among investigators.
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